Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,086,948 C→A 6.1% intergenic (+99/‑54) fdhD → / → yiiG formate dehydrogenase formation protein/DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,086,9480CA 6.1% 1510.6 / 5.0 495intergenic (+99/‑54)fdhD/yiiGformate dehydrogenase formation protein/DUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (138/294);  new base A (49/2);  total (199/296)
Fisher's exact test for biased strand distribution p-value = 4.77e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 506 total aligned reads displayed.

AAACCGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTC  >  NC_000913/4086799‑4087097
                                                                                                                                                     |                                                                                                                                                     
aaaCCGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTc                                                                                                                                                       >  1:2182679/1‑150 (MQ=255)
aaaCCGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTc                                                                                                                                                       >  2:2972801/1‑150 (MQ=255)
aaaCCGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTc                                                                                                                                                       >  2:5060360/1‑150 (MQ=255)
   ccGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTGGTGGCGTTTTCttt                                                                                                                                                    <  2:1560511/150‑1 (MQ=255)
   ccGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCttt                                                                                                                                                    <  1:1861775/150‑1 (MQ=255)
      ggTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCtttttt                                                                                                                                                 <  1:6193790/150‑1 (MQ=255)
      ggTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCtttttt                                                                                                                                                 <  2:3989389/150‑1 (MQ=255)
      ggTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCtttttt                                                                                                                                                 <  1:4217584/150‑1 (MQ=255)
        tCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCa                                                                                                                                               >  2:7302951/1‑150 (MQ=255)
        tCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCa                                                                                                                                               >  2:4535464/1‑150 (MQ=255)
         cGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAt                                                                                                                                              <  2:4329441/150‑1 (MQ=255)
          gggCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAtt                                                                                                                                             <  1:1355240/150‑1 (MQ=255)
           ggCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAttt                                                                                                                                            >  2:1845392/1‑150 (MQ=255)
           ggCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAAttt                                                                                                                                            >  1:3901407/1‑150 (MQ=255)
            gCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAtttt                                                                                                                                           >  1:4955372/1‑149 (MQ=255)
            gCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATtta                                                                                                                                           <  2:6613404/150‑1 (MQ=255)
            gCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATtta                                                                                                                                           <  2:2601107/150‑1 (MQ=255)
            gCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATtta                                                                                                                                           <  1:6824245/150‑1 (MQ=255)
                 ggTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATg                                                                                                                                      <  2:8966540/150‑1 (MQ=255)
                   tttATACCCATCCGCAGCGTTTGAGCACTTAATTAAAATAAGTCCTGCGAAATATATTCCATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGtt                                                                                                                                    <  2:8378266/150‑1 (MQ=255)
                   tttATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGtt                                                                                                                                    <  2:8248730/150‑1 (MQ=255)
                    ttATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTTTAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTc                                                                                                                                   <  1:1016069/150‑1 (MQ=255)
                       taCCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCAct                                                                                                                                <  1:6571347/150‑1 (MQ=255)
                        aCCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTAActc                                                                                                                               >  1:435136/1‑150 (MQ=255)
                          ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTTTGTTAActcta                                                                                                                             >  1:5901758/1‑149 (MQ=255)
                                 cAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCta                                                                                                                      <  1:6380892/150‑1 (MQ=255)
                                  aGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtat                                                                                                                     <  2:8451122/150‑1 (MQ=255)
                                   gCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtata                                                                                                                    <  2:871006/150‑1 (MQ=255)
                                     gTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCATTTTTCATTTATTATTTTCACTCTCATATCtatata                                                                                                                  >  2:9180352/1‑150 (MQ=255)
                                             aattaattAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTTTTTTGTTGAATCTCTTTTATATATATTTATTAg                                                                                                          >  1:5713118/1‑150 (MQ=255)
                                             aattaaatAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAg                                                                                                          <  1:3844821/150‑1 (MQ=255)
                                              attaattaAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAgg                                                                                                         <  1:6671646/150‑1 (MQ=255)
                                               ttaattaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGc                                                                                                        <  1:462185/150‑1 (MQ=255)
                                                taattaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCa                                                                                                       <  2:3374380/150‑1 (MQ=255)
                                                taattaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCa                                                                                                       <  1:5312964/150‑1 (MQ=255)
                                                  attaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAt                                                                                                     >  2:5265651/1‑150 (MQ=255)
                                                  attaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAt                                                                                                     <  2:4144010/150‑1 (MQ=255)
                                                  attaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATGATGTTCACTCTATTATCTGTATATTTATAATGCAAt                                                                                                     >  1:7773390/1‑150 (MQ=255)
                                                    taaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTa                                                                                                   >  1:4520038/1‑150 (MQ=255)
                                                      aaaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTaaa                                                                                                 >  2:4623438/1‑150 (MQ=255)
                                                       aaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                <  1:9045049/150‑1 (MQ=255)
                                                          aaGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaa                                                                                             <  1:7175370/150‑1 (MQ=255)
                                                           aGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaa                                                                                            <  2:1267205/150‑1 (MQ=255)
                                                            gTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                           <  1:7992442/150‑1 (MQ=255)
                                                            gTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                           <  1:8142440/150‑1 (MQ=255)
                                                            gTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                           <  1:2688781/150‑1 (MQ=255)
                                                             tCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGTTTTTTTTTTTTGACTCTCTTTTCTATATATTTTTATGGCAATTAAATTTAAAg                                                                                          >  2:6402355/1‑150 (MQ=255)
                                                             tCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaaa                                                                                          <  2:7879179/150‑2 (MQ=255)
                                                             tCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCTTTTTTCATTTATTATGTTCACTCTCGGATCTATATATTTATAAGGCAAGTAAATGAAAAg                                                                                          >  2:8132975/1‑150 (MQ=255)
                                                                tGCGAAATATATTAAATTGCAGTACATAATCAATTCGTGAATTTTATTATATAATTTATAAACAAAATGCGTGTAGTGGCGTGTTCATATTTGGTTTTATATGATCAAACTCATATAAATATATTTATAATTCCAAAAAATTAAAAGGaa                                                                                       >  1:3230611/1‑150 (MQ=255)
                                                                   gAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGGTCACTCTCTTATCTATATATTTAGAAGGCAAATAAAGGAAAAGGAAatt                                                                                    >  2:113583/1‑150 (MQ=255)
                                                                    aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATGTTGAATCTCGTATATATATAATTATAAGGCAAATAAATGAAAAGGAATtta                                                                                   >  1:7591559/1‑150 (MQ=255)
                                                                     aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTGTTTTTTGATTTTTTTTGTTAAAACTATTATCTATATATTTATTATGGAATTAAATGAAAATGAATttat                                                                                  >  1:5509357/1‑150 (MQ=255)
                                                                      atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttatt                                                                                 <  2:7635696/150‑1 (MQ=255)
                                                                         tatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTGATTTATTATTTTCTCACACTTATATATATAATTTTTATGCAAATAAATGAAAAAGAAATttttata                                                                              >  1:1594288/1‑149 (MQ=255)
                                                                           ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCtt                                                                            >  2:5794549/1‑150 (MQ=255)
                                                                           ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCtt                                                                            >  2:626362/1‑150 (MQ=255)
                                                                           ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTTATTTTTTATTTTGTCTCTATTTTGTATATATTTATAATGGAATTAAATTAAAAGGGATTTATTATAtt                                                                            >  1:2560741/1‑150 (MQ=255)
                                                                             aaaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTcc                                                                          <  1:8879665/150‑1 (MQ=255)
                                                                              aaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCg                                                                         <  1:1303279/150‑1 (MQ=255)
                                                                               aTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGc                                                                        <  2:3655352/150‑1 (MQ=255)
                                                                               aTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGc                                                                        <  2:711911/150‑1 (MQ=255)
                                                                                  gCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtat                                                                     <  2:6808926/150‑1 (MQ=255)
                                                                                         ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTAATTTATTATTTTAACTCACTTTTCTAAATATTTATTATGCAAATAAATGAAAATTTAATTATTATATTACGCTATtatatta                                                              >  1:2281246/1‑147 (MQ=255)
                                                                                         ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                              <  2:6658355/150‑1 (MQ=255)
                                                                                         ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTGATTTTTTATGTTCACTCTGTTATGTATATATTTATTATGCAATTAAATTAAAAGGGATTTATTTTGTTAAGGtattattttg                                                              >  2:7508509/1‑146 (MQ=255)
                                                                                           aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTCACTCTCTTATCTATATATTTATAATGCAATTAAATTAAAATGAATTTATTATCTTACGCTATTATAGTAGc                                                            >  2:6956592/1‑150 (MQ=255)
                                                                                                tttCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcatca                                                       <  2:5995186/150‑1 (MQ=255)
                                                                                                    gTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTc                                                   <  1:5197027/150‑1 (MQ=255)
                                                                                                               tattttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGa                                        <  2:5519135/150‑1 (MQ=255)
                                                                                                                attttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGAc                                       <  1:7284875/150‑1 (MQ=255)
                                                                                                                 ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGTAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACg                                      >  2:3142072/1‑150 (MQ=255)
                                                                                                                 ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAGTTATTATGTTCACTCTCGTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCGCGGTCTGACg                                      >  2:3861647/1‑150 (MQ=255)
                                                                                                                     atttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGtt                                  <  1:8455042/150‑1 (MQ=255)
                                                                                                                     atttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGtt                                  <  2:5745889/150‑1 (MQ=255)
                                                                                                                       ttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgt                                <  1:5675634/150‑1 (MQ=255)
                                                                                                                        tataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgtg                               >  2:592276/1‑150 (MQ=255)
                                                                                                                        tataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATAAATTTATAAGGCAAAAAAATGAAAAGGAATTTATTAACTTACGCTATTATACTCGACATCATGTCCCGCGGTCTGACGGGTtgtg                               >  2:9257146/1‑150 (MQ=255)
                                                                                                                         ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTATTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                              <  2:2279360/150‑1 (MQ=255)
                                                                                                                           aaaCAAAATGCGTGTAGTGGCGTTTTCTTTTTTGATTTATTATGTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTACGCTATTATAGTCGCCATCATGTCCCTCCGTCTGTCGGGTTGtgatt                            >  2:2379217/1‑149 (MQ=255)
                                                                                                                           aaaCAAAATGCGTGTAGTGGCGTGTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTCCGCTATTATAGTCGCCATCATGTACCTCGGTCTGACGGGTTGtgatg                            >  2:496925/1‑150 (MQ=255)
                                                                                                                            aaCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAGTTATTATGTTCACACTCTTATCTATATAATTATAAGGCAAATAAATGAAAAGGAAATTATTATCTTCCCCGATTATAGTCGCCAACATGTCCCTCGGGCGGACGGGGTGtgatga                           >  2:2841101/1‑150 (MQ=255)
                                                                                                                              cAATATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAATTGAAAAGGAATTTATTATCTTCCGCTATTATATTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATa                         <  1:8753046/150‑1 (MQ=255)
                                                                                                                               aaaaTGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaa                        >  2:1388487/1‑150 (MQ=255)
                                                                                                                                aaaTGCGTGTATTGTCTTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaa                       <  1:484821/150‑1 (MQ=255)
                                                                                                                                   tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                    <  1:4655997/150‑1 (MQ=255)
                                                                                                                                     cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                  <  2:5586742/150‑1 (MQ=255)
                                                                                                                                     cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                  <  1:3633460/150‑1 (MQ=255)
                                                                                                                                       tgtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCg                <  2:8621233/150‑1 (MQ=255)
                                                                                                                                        gtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGa               <  1:8299698/150‑1 (MQ=255)
                                                                                                                                              gCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGa         >  2:4933374/1‑150 (MQ=255)
                                                                                                                                              gCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGa         <  1:4317936/150‑1 (MQ=255)
                                                                                                                                              gCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGa         <  2:3199813/150‑1 (MQ=255)
                                                                                                                                              gCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGa         >  2:4832211/1‑150 (MQ=255)
                                                                                                                                               cGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaa        <  1:5055514/150‑1 (MQ=255)
                                                                                                                                               cGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaa        <  1:989489/150‑1 (MQ=255)
                                                                                                                                                 ttttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAAc      <  2:2213136/150‑1 (MQ=255)
                                                                                                                                                   ttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGc    <  1:7033280/150‑1 (MQ=255)
                                                                                                                                                    tCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCt   <  2:6158227/150‑1 (MQ=255)
                                                                                                                                                     cTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTc  >  1:6924453/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCGGGTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTC  >  NC_000913/4086799‑4087097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: