| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,581 | A→C | 20.5% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,581 | 0 | A | C | 20.5% | 185.9 / 19.0 | 112 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (34/53); new base C (4/21); total (38/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 | ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 1:2593872/1‑150 (MQ=255) gTGAGGTGCAAAAAGAGATGAAAGGGCTTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc < 2:71963/150‑1 (MQ=255) gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc < 2:2283210/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 2:1325102/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGGGGGCCGGGGTGAGGGCATCAgcgcg > 2:3003315/1‑150 (MQ=255) gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCgcgcgc > 1:745790/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa > 2:506899/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTAGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcgcc > 2:1931315/1‑149 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:3400426/1‑150 (MQ=255) aTGAAAGGGCGTTATCCCAAACCTGTCTGGCCGGACGACCCGGCAAATCCGGCACCGCCGCGACGGACGACAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCCGCCGCGCGCACgggggccc > 2:3664517/1‑143 (MQ=37) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcgccc > 1:699288/1‑150 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgggggccc > 1:2352665/1‑143 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccc > 2:3974175/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccccc > 1:34187/1‑148 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggacccac > 1:669919/1‑148 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgccccccc > 1:4344904/1‑148 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccac > 1:4344553/1‑148 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCCAAc > 2:3729248/1‑150 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCCAAc > 2:1265371/1‑150 (MQ=38) cccAAACCTGTCTGCGGGTACGACCCGGAAAATACTGCACGGCCGGGACGCACTAAAATGTATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGCGTGGGCCGGGTTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct < 2:2745467/150‑1 (MQ=255) ccAAACTTGTCTGGCCGGACGACCCGGAAAATACTGCCCCGACGCGACGGCCGAAAAATTTTTGGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:1402197/150‑1 (MQ=37) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:3604364/150‑1 (MQ=255) ccAAACATGACCGCCCGGACGACCCGCCAAAAACTCCACCGACGCGACGCACCAAAAAGTATTCGAAAGGTTGGTTTTCACCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:3503004/150‑1 (MQ=37) cAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:684667/150‑1 (MQ=255) gtGTCGGGCGGGACGACCGGGCTAAGACGGCCCCGCGGGGACGGAGGAAAAAGTTTTCGTACGGTTGGTTTGCTCCCTGTCCCTGGGGGAGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCaa < 2:2376622/149‑1 (MQ=255) ctGTCGGGCCGGACGCCCCGCCAAATCCTCCACCCCCGCGCCGGCCCAAAAAGTTTTCGTACGGTGGGTTTCCCCCCCCACCCTGTGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:2376637/149‑1 (MQ=255) ggccgggCCGGACGCCCCGCCAACCCCGCCACCGCCGCGCCGTCCGAAAAAGTATTCGTACGGTTGGTTTACTCCCGCGCCGTGTGGCCGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:825186/145‑1 (MQ=255) cgggCCGGACGACCCGGAAAATACTGCACCGCCGCGACGTACTAAAAAGTATTCGTATGGTTGTTTTACTCCCTCTCCCGGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 1:1772575/148‑1 (MQ=255) gCCGCACGACCCGCCAAATCCTGCACCGCCGCGACGGACGAAAATGTATTGGTTCGGTTGGTTTGCTCCCTCCCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:3784362/150‑1 (MQ=37) ccGGCCGACCCGGCACCCCCTGCCCCGCCGCGCCGGCCGCAAACGTAGTCGTCCGGTTGGTTTTCGCCCTCTCCCGGGGGGAGCGGGCCGGGGGGCGGGCATCAGCGCGCCCGGTCACCCTCACCCTAACCCTCCCCCTCAAGGGAGAgg < 2:2181704/150‑1 (MQ=255) ccGGACGCCCCGGAACATACTCAACCGACGCGCGGGACTAAAATTTATTCGTATGTTTGTTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:1743424/150‑1 (MQ=255) acgacCCGGCAAATCGTGCCCCGACGCGACGGACGAAAAAGTATTGGTAGGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGAc < 2:1387975/150‑1 (MQ=37) tacCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCAACCGAAACCGAAAACACTAACACAAGGGAGAgggggcgg > 1:1043967/2‑146 (MQ=21) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGAACCCCACCCAAAAACACACCCACAAGCGGGTGGGGGCCg > 2:1382402/1‑150 (MQ=25) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGGGCACGGTGAGCCGCGCCCTCACCCGCTACCACAAGGGGGGGGGGGCCgcgg > 1:3019887/1‑147 (MQ=25) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCACACCCGAAACCACTACCAACAGGGGGAGGGGGCCGagc > 1:670004/1‑148 (MQ=25) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCCCCCCCAAACCCGCTCCCCCCAGGGGGGGGGGCCCGagc > 2:3901284/1‑148 (MQ=25) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCGCACCCCAACCCACACCCCCCACGGCGCGGGGGCCGAGCg > 2:4106148/1‑150 (MQ=25) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAGGTGCACCCACCCCCAAACCCACACCCTCAGGGGCGCGGGGCCCgcgcg > 2:3555320/1‑146 (MQ=25) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCTCAACCAAACCCACCCCCGCCAGGGGGGGGGGGCCCATCg > 1:285333/1‑150 (MQ=25) ggaaaaTCCGGCACCGACGCGACGGACGAAAATGTTTTGGTACGGTTGGTTTTCGCCCACACCCGGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:454427/147‑1 (MQ=255) ggCAAATACGGCCCCGCCGCGACGGACGAAAACGTATTGGTATGGTTGGTTTTCCCCCTCCCCCTGGGGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:1698865/150‑1 (MQ=255) gCAAATACGGCACCGACGCGACGGACGACAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:3259764/150‑1 (MQ=255) cAAATACGGCACCGCCGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGTCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 2:2488079/150‑1 (MQ=255) aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa > 2:1898816/1‑150 (MQ=255) aCTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCCCaaa > 2:402767/1‑150 (MQ=255) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGCCACCGCACCCGAACCCACTCCCGCAAGGGGGGGGGGACCGCTAGAGCCCaaaat > 2:2891126/1‑148 (MQ=25) agCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCACCCCAACCCACACCCACAAAGGCGACGGGCCCGCTCCAGcaccacat > 2:2335189/2‑145 (MQ=25) ccccGACGCGACGGACGAAAAATTATTCGTACGGTTGGTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:3586140/148‑1 (MQ=255) cACCGCCGCGACGGACGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCCCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAATtttt < 2:2310358/150‑1 (MQ=255) cACCGACGCGAGGGAGTAAAATTTTTTGTTATGGTTGTTTTTCTCCCTGTCCGGGTGGTTGTGGGCGGGGTTTTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:1238883/150‑1 (MQ=255) cACCGACGCGAGGGACTAAAATGTTTTCTTATGGTTGGTTTTCTCCCTCCCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:1361873/150‑1 (MQ=255) ccGACGCGCCGGACTAAAATTTTTTCTTATGTTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:2734164/150‑1 (MQ=37) gcgacgGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat < 2:299897/150‑1 (MQ=255) gcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACAAACAAAACCACAACCGCAAGGGAGAGGGGGCCCCAAGAGCACAAAATATGAGTGat > 2:493242/1‑150 (MQ=16) gacgAAAATGTATTCGTACGGTTGGTTTCCCCCCCCGCCCTGGGGGTGCGGGGCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 1:3342574/150‑1 (MQ=255) tttttttCGTTGGGTGGGTTTCCCCCCTGCCGGTGTGGGGGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:144562/145‑1 (MQ=255) aaGTATCCTTATGGTTGGTTTTCTCCCCCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:672957/150‑1 (MQ=38) gttttacGTAGGGTTGGTTGTCCCCAACTCCCTGGGGGTGTGGGCGGGGTTGAGTGCATCAGCGCGCACGTTCACCCTCACCCTTACCCTCTCCCTCAAGTGAGATGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt < 1:4002091/144‑1 (MQ=255) agtagtCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt < 2:700314/150‑1 (MQ=255) aGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt < 2:1082003/150‑1 (MQ=255) ttATTCGTATGGTTGTTTTTCTCCCGCTCCGGGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:1645209/149‑1 (MQ=255) gTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:1384084/150‑1 (MQ=255) gTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:1384526/150‑1 (MQ=255) gTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:2194751/150‑1 (MQ=255) gTATACGAACGGTGGGTTTCCCCCCACGCCCGGTGGTCGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:849101/150‑1 (MQ=255) tcgtctggttggttTTCTCCCTCTCCGTGTGGGTGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:339820/144‑1 (MQ=255) cGTATGGTTGTTTTACTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAAc < 1:3739507/150‑1 (MQ=39) tatgtttgtttTACTCCCCCCCCGGGTGGGAGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag < 2:1624059/145‑1 (MQ=255) aGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:3598086/150‑1 (MQ=255) ttggttTTCGCCCACTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 2:3460705/150‑1 (MQ=255) ttggttTTCCCCCTCTCCGTGGGGGAGTGGGCGGGGGTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 2:4026770/150‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 2:2421969/150‑1 (MQ=255) ggttTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:4283046/150‑1 (MQ=255) ggttTTCTCCCTCACCCGGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:4283030/150‑1 (MQ=255) gttTTCTCCCTCGCCGGGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg < 1:1719084/150‑1 (MQ=255) gttTTCACCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg < 1:841908/150‑1 (MQ=255) tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCACCCGAACCCACGCCCCCAAGGGCGCGGGGCCCGCGCGCGCCCCCATGTTGTGAGATATCTTCGTCTGTCGTGTAACAGACGGACAGaaaaac > 2:3900462/1‑148 (MQ=16) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCCCCCGCACCCAAACCCGCGCCCGCCAGGGCGGGGGGCCCGATCGGGCACAAAATTTGAGAGCTATATTAATCGGTCGTGGAAACGAAGAACACAAAAACgg > 2:2882496/1‑150 (MQ=16) ctctcccgGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTTACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:304268/150‑1 (MQ=255) tgGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:2593872/150‑1 (MQ=255) ggggAGCGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:1325102/149‑1 (MQ=255) ggAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:479139/150‑1 (MQ=255) ggAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:3479750/150‑1 (MQ=255) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCAAAACCACACCCACAAGGGCGAGGGGCCCCAGCGAGCACAAATTTTTGGAGATAAAATATTATGTCTTGTAAAAGAAGAAAAGAAAAAACGGCCTTTTCGccagc > 2:665373/1‑147 (MQ=255) gcgggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt < 2:1717567/147‑1 (MQ=255) gCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGACAATCGGGCTTTTGCGCCTGAATAtt < 2:2763974/150‑1 (MQ=255) ggggTGCGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGTTCGAGCACTAATATTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGTAAAGAAAATCGTGCTTTTGGGCCTTAATATTGCg < 1:2587058/150‑1 (MQ=38) ggggTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:847500/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:544449/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAACTTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3221886/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCNGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:2780016/150‑1 (MQ=255) ggTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:497657/150‑1 (MQ=255) ggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:497656/150‑1 (MQ=255) ggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:2061802/150‑1 (MQ=255) gcgggCCTCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTGGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 1:3098685/148‑1 (MQ=255) gAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 2:3790083/150‑1 (MQ=255) gAGGGAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 2:3014715/150‑1 (MQ=255) gggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:346152/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:597062/150‑1 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |