Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 775,627 G→A 5.1% V159I (GTA→ATA)  tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,6270GA 5.1% 1103.7 / 29.8 312V159I (GTA→ATA) tolQmembrane spanning protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base G (158/137);  new base A (2/14);  total (160/152)
Fisher's exact test for biased strand distribution p-value = 1.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

CGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACAAC  >  NC_000913/775478‑775776
                                                                                                                                                     |                                                                                                                                                     
cgcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCgg                                                                                                                                                       <  2:2318378/150‑1 (MQ=255)
cgcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCgg                                                                                                                                                       <  2:1939567/150‑1 (MQ=255)
cgcgTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCgg                                                                                                                                                       <  2:2649990/150‑1 (MQ=255)
   gTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaa                                                                                                                                                    <  1:3631403/150‑1 (MQ=255)
    tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaa                                                                                                                                                   <  1:2223620/150‑1 (MQ=255)
    tGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTGGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCCGAGGGCGATaaa                                                                                                                                                   <  2:2132335/150‑1 (MQ=255)
     gcgaTGCGTATCTCCAGGCACCGTGAACTTGAAAATCTGGACACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaag                                                                                                                                                  <  2:4079267/147‑2 (MQ=255)
       tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACa                                                                                                                                                >  1:2330934/1‑150 (MQ=255)
        aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACaa                                                                                                                                               <  1:652828/150‑1 (MQ=255)
          gCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGc                                                                                                                                             <  2:2746096/150‑1 (MQ=255)
                 tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTg                                                                                                                                      >  1:4120607/1‑150 (MQ=255)
                 tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTg                                                                                                                                      >  2:595564/1‑150 (MQ=255)
                   cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCa                                                                                                                                    >  1:3898102/1‑150 (MQ=255)
                    aTGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCaa                                                                                                                                   >  1:3852750/1‑150 (MQ=255)
                          cGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGtt                                                                                                                             >  1:1891696/1‑150 (MQ=255)
                          cGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGtt                                                                                                                             >  1:1862416/1‑150 (MQ=255)
                          cGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGtt                                                                                                                             >  1:3917778/1‑150 (MQ=255)
                             taaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACTGTCTGGGGTATCATGCACGCCTTTATCGCCCTCGTGGCGTTATTACAAGCATCACTGCTAATTGTTgcg                                                                                                                          <  2:1366741/149‑1 (MQ=255)
                                 ttGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCg                                                                                                                      >  2:527795/1‑150 (MQ=255)
                                     aaaTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTAt                                                                                                                  >  2:1779385/1‑150 (MQ=255)
                                        tCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGc                                                                                                               <  2:2463142/150‑1 (MQ=255)
                                         cTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCa                                                                                                              <  2:2850875/150‑1 (MQ=255)
                                          tGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAg                                                                                                             >  1:3104428/1‑150 (MQ=255)
                                            gAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGaa                                                                                                           <  2:3493592/150‑1 (MQ=255)
                                             aaaCGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAg                                                                                                          >  2:3960630/1‑150 (MQ=255)
                                                cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGt                                                                                                       <  2:4254440/150‑1 (MQ=255)
                                                   acaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGa                                                                                                    <  1:1727993/150‑1 (MQ=255)
                                                    caTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGAt                                                                                                   <  1:298926/150‑1 (MQ=255)
                                                      ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTg                                                                                                 >  1:1982345/1‑150 (MQ=255)
                                                      ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTg                                                                                                 >  2:77995/1‑150 (MQ=255)
                                                      ttCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTg                                                                                                 >  1:2712461/1‑150 (MQ=255)
                                                        ccGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCg                                                                                               <  2:3818661/150‑1 (MQ=255)
                                                         cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGa                                                                                              <  1:4249283/150‑1 (MQ=255)
                                                           ttCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACt                                                                                            <  1:238436/150‑1 (MQ=255)
                                                               tCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTGTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTGGAGTGCGACGGCaa                                                                                        >  2:2793497/1‑150 (MQ=255)
                                                                cGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAAt                                                                                       <  1:3591833/150‑1 (MQ=255)
                                                                cGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAAt                                                                                       <  1:2539099/150‑1 (MQ=255)
                                                                      ggTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCt                                                                                 <  2:3909711/150‑1 (MQ=255)
                                                                      ggTTGGCTCCATCAGCCCGTAGATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCt                                                                                 >  2:1041870/1‑150 (MQ=255)
                                                                       gTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCGCTCGGGGCGGTAAAACAAGCATCACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTg                                                                                <  2:2542250/150‑1 (MQ=255)
                                                                       gTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTg                                                                                >  1:3768426/1‑150 (MQ=255)
                                                                       gTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTg                                                                                <  1:2026436/150‑1 (MQ=255)
                                                                           gCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTg                                                                            >  1:3177746/1‑150 (MQ=255)
                                                                            cTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGc                                                                           >  2:1156561/1‑150 (MQ=255)
                                                                             tCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGcc                                                                          >  1:3262848/1‑150 (MQ=255)
                                                                              ccATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCg                                                                         <  2:1809342/150‑1 (MQ=255)
                                                                              ccATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCg                                                                         <  2:1609155/150‑1 (MQ=255)
                                                                              ccATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCg                                                                         <  2:4111734/150‑1 (MQ=255)
                                                                                   aGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATc                                                                    >  2:870641/1‑150 (MQ=255)
                                                                                    gCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATcc                                                                   <  1:787865/150‑1 (MQ=255)
                                                                                     cccGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATccc                                                                  <  1:1414056/150‑1 (MQ=255)
                                                                                              tGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTAt                                                         >  1:2834600/1‑150 (MQ=255)
                                                                                               ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATg                                                        <  2:3731412/150‑1 (MQ=255)
                                                                                               ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATg                                                        <  2:3080665/150‑1 (MQ=255)
                                                                                               ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATg                                                        <  2:1102591/150‑1 (MQ=255)
                                                                                                gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                       >  1:297705/1‑150 (MQ=255)
                                                                                                gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                       <  1:2214177/150‑1 (MQ=255)
                                                                                                gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                       <  2:2490315/150‑1 (MQ=255)
                                                                                                     tttGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTAc                                                  >  1:1654581/1‑150 (MQ=255)
                                                                                                       tGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACaa                                                <  1:2807865/150‑1 (MQ=255)
                                                                                                       tGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACaa                                                <  2:1509647/150‑1 (MQ=255)
                                                                                                        ggTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAc                                               >  2:2171880/1‑150 (MQ=255)
                                                                                                         gTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAcc                                              <  1:886381/150‑1 (MQ=255)
                                                                                                         gTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAcc                                              <  1:727113/150‑1 (MQ=255)
                                                                                                         gTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAcc                                              <  2:1544883/150‑1 (MQ=255)
                                                                                                         gTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAcc                                              >  1:1302427/1‑150 (MQ=255)
                                                                                                          tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                                             >  2:3642816/1‑150 (MQ=255)
                                                                                                          tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                                             >  1:1284138/1‑150 (MQ=255)
                                                                                                           aCGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGc                                            <  1:4339379/150‑1 (MQ=255)
                                                                                                                 tGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAc                                      <  1:1099943/150‑1 (MQ=255)
                                                                                                                 tGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAc                                      >  2:3189696/1‑150 (MQ=255)
                                                                                                                  gggggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAcc                                     <  2:4323019/150‑1 (MQ=255)
                                                                                                                   ggggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCa                                    >  1:1929268/1‑150 (MQ=255)
                                                                                                                     ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgc                                  >  2:2999059/1‑150 (MQ=255)
                                                                                                                       aTCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgcgc                                >  2:1290961/1‑150 (MQ=255)
                                                                                                                        tCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGcgcg                               >  1:2795209/1‑150 (MQ=255)
                                                                                                                        tCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGcgcg                               <  1:4028731/150‑1 (MQ=255)
                                                                                                                          aTGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTa                             <  1:947258/150‑1 (MQ=255)
                                                                                                                             cACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaac                          >  1:3377004/1‑150 (MQ=255)
                                                                                                                              aCGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaaca                         >  1:1043824/1‑150 (MQ=255)
                                                                                                                                gCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaa                       >  1:1521580/1‑150 (MQ=255)
                                                                                                                                 ccTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaac                      <  1:715838/150‑1 (MQ=255)
                                                                                                                                   tttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTCAACAAACTg                    >  1:1859992/1‑150 (MQ=255)
                                                                                                                                   tttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTg                    >  2:2955359/1‑150 (MQ=255)
                                                                                                                                   tttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTg                    >  2:917032/1‑150 (MQ=255)
                                                                                                                                      aTCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCCATTGGTCTGTTTGCCGCTCTCCCGGCAGTTATGGCCTACAACCGCCTCAACCCGCGCGTAAACAAACTGGac                 >  2:2216980/1‑149 (MQ=255)
                                                                                                                                      aTCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTGATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGaa                 >  2:3277523/1‑150 (MQ=255)
                                                                                                                                       tCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCCGCGCGTAAACAAACTGGAAc                >  2:2044905/1‑150 (MQ=255)
                                                                                                                                       tCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAAc                >  2:2103000/1‑150 (MQ=255)
                                                                                                                                        cGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACt               <  1:535604/150‑1 (MQ=255)
                                                                                                                                          cccTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGa             >  1:3980286/1‑150 (MQ=255)
                                                                                                                                              cGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTa         <  1:3445398/150‑1 (MQ=255)
                                                                                                                                              cGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTa         <  2:1390373/150‑1 (MQ=255)
                                                                                                                                              cGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTa         <  2:1862416/150‑1 (MQ=255)
                                                                                                                                              cGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTa         <  2:1891696/150‑1 (MQ=255)
                                                                                                                                                gggCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACg       >  1:3703026/1‑150 (MQ=255)
                                                                                                                                                gggCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACg       >  2:3426040/1‑150 (MQ=255)
                                                                                                                                                  gCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGAc     <  1:3723990/150‑1 (MQ=255)
                                                                                                                                                  gCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGAc     <  1:484474/150‑1 (MQ=255)
                                                                                                                                                     gTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACAAc  >  1:2696551/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACAAC  >  NC_000913/775478‑775776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: