Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 6.7% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 6.7% 498.2 / 2.6 169L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (85/65);  new base T (2/14);  total (88/81)
Fisher's exact test for biased strand distribution p-value = 9.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238376‑238672
                                                                                                                                                   |                                                                                                                                                     
tATTGACATATCCTGTGACAAATGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATCgtaagta                                                                                                                                                     >  2:3547115/1‑150 (MQ=255)
tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                                     >  2:813141/1‑150 (MQ=255)
  ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                                   >  1:3172225/1‑150 (MQ=255)
  ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                                   <  2:1079722/150‑1 (MQ=255)
   tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                                  <  2:512466/150‑1 (MQ=255)
   tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                                  <  1:4302591/150‑1 (MQ=255)
    gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                                 <  2:3057994/150‑1 (MQ=255)
     aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                >  1:2192834/1‑150 (MQ=255)
      cATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTAGAATTATTTTATTATAGTAAGTAAAATTa                                                                                                                                               >  1:1617557/1‑150 (MQ=255)
            cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         >  1:3220169/1‑150 (MQ=255)
            cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         <  1:2156473/150‑1 (MQ=255)
             tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        <  1:2034324/150‑1 (MQ=255)
                 aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                    >  1:1121347/1‑150 (MQ=255)
                     aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTaaaa                                                                                                                                >  2:2714916/1‑150 (MQ=255)
                      aGGAGCTATTAATGGTGCTATTACTATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:2522947/150‑1 (MQ=255)
                      aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:2043006/150‑1 (MQ=255)
                      aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:1696506/150‑1 (MQ=255)
                      aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:3412639/150‑1 (MQ=255)
                      aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  2:4085841/150‑1 (MQ=255)
                      aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTATTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:2522924/150‑1 (MQ=255)
                       ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  1:2475812/1‑150 (MQ=255)
                       ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              <  2:4321834/150‑1 (MQ=255)
                       ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  1:870736/1‑150 (MQ=255)
                           cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                          <  2:508722/150‑1 (MQ=255)
                            tATTAAAGGTGCTATTACGATAGCTATTATTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                         <  1:1225204/150‑1 (MQ=255)
                                        tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                             >  1:2198225/1‑150 (MQ=255)
                                            aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAAAGACATGAAACCGAAg                                                                                                         >  1:1981086/1‑150 (MQ=255)
                                            aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                         >  1:314400/1‑150 (MQ=255)
                                               aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                      <  2:3996115/150‑1 (MQ=255)
                                               aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                      <  2:1805239/150‑1 (MQ=255)
                                               aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                      <  2:1230235/150‑1 (MQ=255)
                                                tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                     >  2:1677764/1‑150 (MQ=255)
                                                tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                     >  2:2183350/1‑150 (MQ=255)
                                                   cTATTTGTAAAAATATAAGAGTTTGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                  <  2:3583491/150‑1 (MQ=255)
                                                   cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcacacac                                                                                                  >  1:4100820/1‑147 (MQ=255)
                                                    tATTAGTAAAAATATAAGATTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 <  2:3231415/150‑1 (MQ=255)
                                                    tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 <  2:2713382/150‑1 (MQ=255)
                                                    tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 >  2:1545355/1‑150 (MQ=255)
                                                     tttataaaaatTATAATTTTTAGTTGTTTTTTTTTGTGTGTGGCTATTTTCAATACTTTGTTTTTTTTTTTTAATTTTTATTTTTTTATCTTATCTAAATTTAAATCAATTTTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATc                                                                                                <  1:1486952/144‑1 (MQ=255)
                                                     aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                <  2:3835679/150‑1 (MQ=255)
                                                      tttttAAAAATATAATAGTTTGCTTTTTTGTTATGTCTGTTGCGTTTTTGACTACCTTCGTTTTTTTTATTAAGTATGATTTTTTTATGGTATTTATAATTACTTGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCa                                                                                               <  2:784418/146‑1 (MQ=255)
                                                        tgTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTATGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                             <  2:1993592/149‑1 (MQ=255)
                                                         gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                            >  2:3450367/1‑150 (MQ=255)
                                                           aaaaaTATAAGAGTTAGATGTATTGTTATTTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                          <  2:2621814/150‑1 (MQ=255)
                                                            aaaaTATATGATTTAGCTTTTTTTTTTTTTCTGTGGCTATATTGAATAACTTCTTTTTTTTTATTAATATTTTTTTTATTTTCTTAATAAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         <  1:471083/150‑1 (MQ=255)
                                                            aaaaTATAAGATTTAGATTTATTGTTTTGTCTGTGGCGAAATTTACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTAACTTAATTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         <  1:471145/150‑1 (MQ=255)
                                                            aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAGGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         >  2:2826126/1‑150 (MQ=255)
                                                             aaaTTTAAGTGTTTGCTGTTTTTTTTTGTCTGGGGCTAATTTTACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATCGTATTTAAATTTACTTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                        <  1:4263570/150‑1 (MQ=255)
                                                             aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                        <  2:1463641/150‑1 (MQ=255)
                                                              aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATCTTTAAAAAGGAAAACGACAGGAAACCGACGCGCAGACTCAACATtctc                                                                                       >  1:3599489/1‑150 (MQ=255)
                                                              aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAATCAACATtctc                                                                                       >  1:3130463/1‑150 (MQ=255)
                                                              aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                       >  1:948398/1‑150 (MQ=255)
                                                                  taAGATTTAGCTTTATTTTTTTGTCTTTGGCCAATTTGACAACCTTCTTTTTTTTTATAAATAATTATTTTATTATCTTAACTAAAATTAAATTAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   <  1:4108042/150‑1 (MQ=255)
                                                                   aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTATcccaac                                                                                  >  1:1334538/1‑146 (MQ=255)
                                                                   aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAATTGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTGCAATc                                                                                  >  2:659281/1‑150 (MQ=255)
                                                                   aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAGAATAAACAATCTACAAta                                                                                  >  1:1605483/1‑149 (MQ=255)
                                                                     gagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATAGTAAGTAAAAATAAATGAATAATTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAAACAACATTATACaataat                                                                                >  1:1349112/1‑150 (MQ=255)
                                                                     gagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAt                                                                                >  2:2144829/1‑150 (MQ=255)
                                                                      agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                                               >  2:2544087/1‑150 (MQ=255)
                                                                            cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                         >  2:903970/1‑150 (MQ=255)
                                                                            cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATaaaaaaa                                                                         >  2:4164697/1‑147 (MQ=255)
                                                                            cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                         >  2:245452/1‑150 (MQ=255)
                                                                             tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                        >  2:2343570/1‑150 (MQ=255)
                                                                             tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                        >  2:1824926/1‑150 (MQ=255)
                                                                             tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                        >  2:2774/1‑150 (MQ=255)
                                                                             tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAAAAAATAtt                                                                        >  2:3461544/1‑150 (MQ=255)
                                                                                  ttttATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                   <  1:1384232/148‑1 (MQ=255)
                                                                                  tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAATATTACATGAATATTTAAAATTGAAATCGTCATGAAACCGAAGCACAGTATCAACATTCTCCAATCATAATATATTTACGt                                                                   >  2:1474601/1‑150 (MQ=255)
                                                                                    ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                 >  2:676303/1‑150 (MQ=255)
                                                                                          cTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGTAAAAGACATGAAACCGAAGCACAGAAACAAAAATAAACAATAAAAAAAAATTTACGTGGAGCAtt                                                           >  1:3826430/1‑150 (MQ=255)
                                                                                          cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTAATATAGTAAGTAAAAATAAATGAATATTTAAAAAGGAAAACGACAATAAACCGAACCACAGAAAAAAAATTCTCCAATAATAAAATATTTACGGGGAGCAtt                                                           >  1:3826262/1‑150 (MQ=255)
                                                                                           tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                          >  2:3030036/1‑150 (MQ=255)
                                                                                           tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                          >  2:3535267/1‑150 (MQ=255)
                                                                                             tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        <  1:288940/150‑1 (MQ=255)
                                                                                               gagAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAGGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTATCCAATAATAAAATATTTCCGTGGAGCATTttatt                                                      >  2:2747691/3‑150 (MQ=255)
                                                                                               gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCCATCATAAAATATTTCCGTGGAGCATTttatt                                                      >  2:158890/1‑150 (MQ=255)
                                                                                                 gAAATTGACTACCTTCGTTTTTTTGTTTTAGAATTATTTTATTATAGTAAGTAAAATTACAATAATATTTAAAAAGGAAAACGACAATAAAACGAAGCACAGAATAAACAATATACAAAAATAAAATATTTTAGTGGGGCAATttatttt                                                    >  1:2258574/1‑148 (MQ=255)
                                                                                                     ttGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:2858356/150‑1 (MQ=255)
                                                                                                         cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACATTATCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                            >  2:3380831/1‑150 (MQ=255)
                                                                                                          tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                           <  1:3653535/150‑1 (MQ=255)
                                                                                                                  ttttttttATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                   <  1:2797171/150‑1 (MQ=255)
                                                                                                                  tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                   >  2:4153737/1‑150 (MQ=255)
                                                                                                                   ttttttGATTAAGAATTATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:442280/150‑1 (MQ=255)
                                                                                                                   ttttttGATTAAGAATGATTTTATTATATTAAGTAAAATTACTTGAATATTTAAAATTGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTatc                                  <  2:2206898/150‑3 (MQ=255)
                                                                                                                    tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACt                                 >  1:3459330/1‑150 (MQ=255)
                                                                                                                     ttttTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                <  2:58166/150‑1 (MQ=255)
                                                                                                                      tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               >  1:2978837/1‑150 (MQ=255)
                                                                                                                       ttGATTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                              <  1:4018630/150‑1 (MQ=255)
                                                                                                                           ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGtca                          >  1:1037412/1‑148 (MQ=255)
                                                                                                                                 aTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                    <  1:1028142/150‑1 (MQ=255)
                                                                                                                                  tGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                   <  2:3541946/150‑1 (MQ=255)
                                                                                                                                  tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                   <  2:2430968/150‑1 (MQ=255)
                                                                                                                                  tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                   >  2:3875932/1‑150 (MQ=255)
                                                                                                                                   gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  >  2:551312/1‑150 (MQ=255)
                                                                                                                                   gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:2493235/150‑1 (MQ=255)
                                                                                                                                     ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  1:2113801/1‑150 (MQ=255)
                                                                                                                                       ttattaTCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag              <  2:3690145/150‑1 (MQ=255)
                                                                                                                                             tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa        >  1:3895438/1‑150 (MQ=255)
                                                                                                                                              cttaagtaaAATTAAATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  1:848957/148‑1 (MQ=255)
                                                                                                                                                taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     >  1:603503/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238376‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: