Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,535 G→T 5.9% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5350GT 5.9% 553.8 / 2.6 184H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (92/73);  new base T (4/14);  total (96/88)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238388‑238684
                                                                                                                                                   |                                                                                                                                                     
cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                     <  2:458986/150‑1 (MQ=255)
cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCCGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                     >  1:3220101/1‑150 (MQ=255)
  gtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAata                                                                                                                                                   <  1:2143556/150‑1 (MQ=255)
     aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                >  1:1121347/1‑150 (MQ=255)
     aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                >  1:1837568/1‑150 (MQ=255)
         aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATATTAAGTAAAATTAAATGAATATTTaaaa                                                                                                                                            >  2:774053/1‑150 (MQ=255)
          aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:1471935/150‑1 (MQ=255)
          aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:3412603/150‑1 (MQ=255)
          aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:2043006/150‑1 (MQ=255)
          aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTATTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:2522924/150‑1 (MQ=255)
           ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                          <  2:4321978/150‑1 (MQ=255)
           ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                          >  1:2475812/1‑150 (MQ=255)
           ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                          >  1:870736/1‑150 (MQ=255)
               cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                      <  1:852840/150‑1 (MQ=255)
                tATTAAAGGTGCTATTACGATAGCTATTATTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     <  1:1225204/150‑1 (MQ=255)
                tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     >  1:2032929/1‑150 (MQ=255)
                      aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAt                                                                                                                               <  2:3265690/150‑1 (MQ=255)
                           cTATTACTATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                                          <  2:2165471/150‑1 (MQ=255)
                                aCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAg                                                                                                                     >  1:314400/1‑150 (MQ=255)
                                    tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                                 >  2:2183350/1‑150 (MQ=255)
                                       cTATTTGTAAAAATATAAGAGTTTGCTGTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                              <  2:3583491/150‑1 (MQ=255)
                                        tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                             <  2:2713382/150‑1 (MQ=255)
                                        tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                             >  2:1545355/1‑150 (MQ=255)
                                         tttataaaaatTATAATTTTTAGTTGTTTTTTTTTGTGTGTGGCTATTTTCAATACTTTGTTTTTTTTTTTTAATTTTTATTTTTTTATCTTATCTAAATTTAAATCAATTTTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATc                                                                                                            <  1:1486952/144‑1 (MQ=255)
                                         aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                            <  2:3835679/150‑1 (MQ=255)
                                             gTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACa                                                                                                        >  2:3450367/1‑150 (MQ=255)
                                               aaaaaTATAAGAGTTAGATGTATTGTTATTTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                      <  2:2621814/150‑1 (MQ=255)
                                                aaaaTATAAGATTTAGATTTATTGTTTTGTCTGTGGCGAAATTTACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTAACTTAATTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                     <  1:471145/150‑1 (MQ=255)
                                                aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAGGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                     >  2:1246916/1‑150 (MQ=255)
                                                 aaaTTTAAGTGTTTGCTGTTTTTTTTTGTCTGGGGCTAATTTTACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATCGTATTTAAATTTACTTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                    <  1:4263570/150‑1 (MQ=255)
                                                 aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                    <  2:1463743/150‑1 (MQ=255)
                                                 aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACAAGAATATTTAAAAAGGAAAACCACAAGAAACCCAAGCACAGAAacaacaataa                                                                                                    >  1:432985/1‑146 (MQ=255)
                                                 aaaGATAAGAGTTAGCTGTATTGTTATGTCTCTGGCGAAATTCACTACCTTCGTTTTTTTGATTAACAATGATTTTATTATCCTAAGTAAAATTACAGGACAATTTAAAAAGGAACAAGACATGAAACCGCAGCACAGAATCAACaagct                                                                                                    >  2:2574560/1‑146 (MQ=255)
                                                  aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATCTTTAAAAAGGAAAACGACAGGAAACCGACGCGCAGACTCAACATtctc                                                                                                   >  1:3599489/1‑150 (MQ=255)
                                                      taAGATTTAGCTTTATTTTTTTGTCTTTGGCCAATTTGACAACCTTCTTTTTTTTTATAAATAATTATTTTATTATCTTAACTAAAATTAAATTAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                               <  1:4108042/150‑1 (MQ=255)
                                                      taAGAGTTAGCTGTATTGTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                               <  2:2752459/150‑1 (MQ=255)
                                                       aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTATcccaac                                                                                              >  1:1334538/1‑146 (MQ=255)
                                                       aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAATTGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTGCAATc                                                                                              >  2:659281/1‑150 (MQ=255)
                                                          agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                                                           >  2:2543979/1‑150 (MQ=255)
                                                          agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACAGAAGCACAGAATCAACATTCTCCAATCATa                                                                                           >  2:2972847/1‑150 (MQ=255)
                                                          agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACAAGAAAACGAAGCACAGAATAAACATTATCCAataata                                                                                           >  1:2658811/1‑150 (MQ=255)
                                                                cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                     >  2:531478/1‑150 (MQ=255)
                                                                cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                     >  2:903970/1‑150 (MQ=255)
                                                                cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATaaaaaaa                                                                                     >  2:4164697/1‑147 (MQ=255)
                                                                cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                                     >  2:245452/1‑150 (MQ=255)
                                                                 tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                                    >  2:1824926/1‑150 (MQ=255)
                                                                 tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAAAAAATAtt                                                                                    >  2:3461544/1‑150 (MQ=255)
                                                                  ttttttttATGTCTGTGGCTAAATTGAATACTTTCTTTTTTTTTATTAATATTTTTTTTATTATGTTAATTAAAATTACATGAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                   <  1:1630600/144‑1 (MQ=255)
                                                                  gTATTGTTATGTCTGTGGCTAAATTTAATACATTCGTTTTTTTTATTAATAATGATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                                   <  2:3889685/150‑1 (MQ=255)
                                                                      tGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                               <  1:671590/150‑1 (MQ=255)
                                                                       tttttGTCTGTGGCTAATTTTAATACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                              <  1:2962991/146‑1 (MQ=255)
                                                                        ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTgg                                                                             >  2:281724/1‑150 (MQ=255)
                                                                         tttttCTGTGGATAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                            <  1:3007639/146‑1 (MQ=255)
                                                                          aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                           >  2:3196577/1‑150 (MQ=255)
                                                                             tCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTAGAATTATTTTTTTATAGTAAATAAAATTAAATTAAAATTTAAAAAGGAAAAACAAAAGAAAACCAAGCACAGAAACAAAAATATACAAACAAAAAAAAATTACGTGGTGcct                                                                        >  1:1377157/1‑148 (MQ=255)
                                                                              cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTAATATAGTAAGTAAAAATAAATGAATATTTAAAAAGGAAAACGACAATAAACCGAACCACAGAAAAAAAATTCTCCAATAATAAAATATTTACGGGGAGCAtt                                                                       >  1:3826262/1‑150 (MQ=255)
                                                                               tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                      >  2:3535317/1‑150 (MQ=255)
                                                                               tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                      >  2:3030036/1‑150 (MQ=255)
                                                                                 tgGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTATATTAAAATTACTTGAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                    <  1:2845017/150‑1 (MQ=255)
                                                                                   gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCCATCATAAAATATTTCCGTGGAGCATTttatt                                                                  >  2:158890/1‑150 (MQ=255)
                                                                                   gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                  >  2:2747184/1‑150 (MQ=255)
                                                                                     gAAATTGACTACCTTCGTTTTTTTGTTTTAGAATTATTTTATTATAGTAAGTAAAATTACAATAATATTTAAAAAGGAAAACGACAATAAAACGAAGCACAGAATAAACAATATACAAAAATAAAATATTTTAGTGGGGCAATttatttt                                                                >  1:2258574/1‑148 (MQ=255)
                                                                                         ttGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                            <  2:2858356/150‑1 (MQ=255)
                                                                                         ttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                            >  2:4286772/1‑150 (MQ=255)
                                                                                             cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACAAAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                                        >  2:1987270/1‑150 (MQ=255)
                                                                                              tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                                       <  1:3653535/150‑1 (MQ=255)
                                                                                                      tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                               >  2:2281323/1‑150 (MQ=255)
                                                                                                         ttttTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                            <  2:58166/150‑1 (MQ=255)
                                                                                                          tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           >  1:3728705/1‑150 (MQ=255)
                                                                                                           ttGATTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          <  1:4018630/150‑1 (MQ=255)
                                                                                                           ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                          <  1:3165548/150‑1 (MQ=255)
                                                                                                               ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGtca                                      >  1:1037412/1‑148 (MQ=255)
                                                                                                                   gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                                  >  1:3924816/1‑150 (MQ=255)
                                                                                                                     aTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                                <  1:1028142/150‑1 (MQ=255)
                                                                                                                      tGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                               <  2:3541946/150‑1 (MQ=255)
                                                                                                                       taTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  1:2172741/149‑1 (MQ=255)
                                                                                                                       gATTTTTTTATCGTATGTAAAATTACATGAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  1:4164697/150‑1 (MQ=255)
                                                                                                                       gATTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:4093225/150‑1 (MQ=255)
                                                                                                                       gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAACATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:2352880/150‑1 (MQ=255)
                                                                                                                       gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              >  2:551312/1‑150 (MQ=255)
                                                                                                                       gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              >  2:1627605/1‑150 (MQ=255)
                                                                                                                       gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                              <  2:2013253/150‑1 (MQ=255)
                                                                                                                        aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                             >  1:3478068/1‑150 (MQ=255)
                                                                                                                         ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            >  1:2229473/1‑150 (MQ=255)
                                                                                                                         ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            >  1:2113801/1‑150 (MQ=255)
                                                                                                                         ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            >  2:1727059/1‑150 (MQ=255)
                                                                                                                          tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa                           >  2:4007679/1‑150 (MQ=255)
                                                                                                                           ttattaTCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag                          <  2:3690145/150‑1 (MQ=255)
                                                                                                                              ttatCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt                       <  2:784625/150‑1 (MQ=255)
                                                                                                                                    taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg                 >  2:1018080/1‑150 (MQ=255)
                                                                                                                                       ttaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  1:2714916/149‑1 (MQ=255)
                                                                                                                                       ttaaAATTAAATTAATATTTAAAAATTAACACGACATGAAACCGATTCACATAATCAACATTCTCCAATCATAAAATATTTCCGTGTAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  1:774053/149‑1 (MQ=255)
                                                                                                                                       gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  1:3889177/150‑1 (MQ=255)
                                                                                                                                        taaAATTACATGGATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg             >  2:1221210/1‑150 (MQ=255)
                                                                                                                                        taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg             >  2:1805437/1‑150 (MQ=255)
                                                                                                                                                 aTGAATATTTAAAAAGGAAAACGACATGACACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    <  1:2635309/150‑1 (MQ=255)
                                                                                                                                                 aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    <  2:4060529/150‑1 (MQ=255)
                                                                                                                                                 aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    >  2:3020510/1‑150 (MQ=255)
                                                                                                                                                 aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    >  1:3416587/1‑150 (MQ=255)
                                                                                                                                                   gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAataata  <  1:159016/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238388‑238684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: