Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 14.5% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA14.5% 472.8 / 13.8 204intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (73/89);  new base A (26/11);  total (104/100)
Fisher's exact test for biased strand distribution p-value = 6.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383
                                                                                                                                                    |                                                                                                                                                      
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                       <  1:4243563/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTTTTCAGGATCTGCTTTGTCGTTACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCaa                                                                                                                                                      <  2:2648229/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                      <  2:1344598/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                      <  2:1014835/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                   >  2:671442/1‑150 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                  <  2:129487/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAatat                                                                                                                                                 >  2:1278349/1‑150 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                             <  1:961962/150‑1 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATg                                                                                                                                             >  1:19371/1‑150 (MQ=255)
           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                            >  2:3768411/1‑150 (MQ=255)
            tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGaa                                                                                                                                           >  2:2114008/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                         >  2:209968/1‑150 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                       <  1:2143361/150‑1 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCTGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTAGTCGATACCACGTACATATTCATATCCTGTCGTTTTTTTTCGAATTCAAAAAAAAATGAAaataa                                                                                                                                      >  2:2572213/1‑149 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCATTATATTTTGAAatcga                                                                                                                                      <  1:2261046/150‑4 (MQ=255)
                  acgacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                     <  1:2456108/147‑1 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                    <  1:2178221/150‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAAATAAAATAtttt                                                                                                                                   >  1:3642624/1‑149 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                  <  2:2309435/150‑1 (MQ=255)
                        tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                               <  2:3766067/150‑1 (MQ=255)
                        tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                               <  2:3337670/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                        <  2:1469489/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAatta                                                                                                                        >  2:2628916/1‑148 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                     <  2:3127075/150‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                    <  1:903094/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             >  2:652465/1‑150 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  1:2797695/150‑1 (MQ=255)
                                          tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                            <  2:47968/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           >  2:1412283/1‑150 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  2:283134/150‑1 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  1:4182187/150‑1 (MQ=255)
                                                ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  1:942145/150‑1 (MQ=255)
                                                  gactGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTGAGCATCTGCTTTGTCGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                    <  2:3305143/147‑1 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:2517432/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATGGAGATAAAGATAACAAAAACg                                                                                                 >  1:1423609/1‑150 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGAc                                                                                               <  2:2593731/150‑1 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGCTCACAAAAACGAc                                                                                               >  2:2342406/1‑150 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTAAATATAGAGAAAAATATAAAAAAAAAGACa                                                                                              >  2:3721231/1‑150 (MQ=255)
                                                          gAACGGCATAACCGCCGCGCTGTGCGTTGTTCAGCTTCTGCTTTGTCGATACCACGTACATTTTCTTATCCTGTCGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTATCTTTCGAGATAACGATCACAAAAACGACaag                                                                                            <  2:3567343/150‑2 (MQ=255)
                                                             ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGGGATAACGATCACAAAAACGACAaaatt                                                                                         >  1:2826149/1‑149 (MQ=255)
                                                              cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATAAAATTTAATTATTTTTTTTTAAATATAGATATAACGGTAACAAAAACGAcccaatga                                                                                        >  1:3368876/1‑143 (MQ=255)
                                                              cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACaaaaata                                                                                        >  1:2659168/1‑145 (MQ=255)
                                                              cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAATGAAAATATTTTTTTTTAAATATACATGTAAAGATAAAAAAAAAGACAAAATGa                                                                                        >  1:1619251/1‑150 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATgga                                                                                       >  2:195675/1‑148 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                       >  2:365036/1‑150 (MQ=255)
                                                               gCATAACCGCCGCGCAGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAAAGAAAAAAATTTTTTTTAAAAATACAGATAAAGAACAAAAAAAACACAataatga                                                                                       >  1:1059954/1‑146 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAGTTAAAAATATAAAGAAATTATTTTTTTTTAAATATAGAGATAACGATACCAAAAACGCCACAAAGAAAat                                                                                    >  1:3503371/1‑150 (MQ=255)
                                                                         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                             <  2:4329930/150‑1 (MQ=255)
                                                                           cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAATATAGATATAACGATAACAAAAACGAAAAGATTAAAaatattatagg                                                                           >  1:4058756/1‑145 (MQ=255)
                                                                           cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGTTTTGAAAAAATAAAGAAATTATTTTTTTTTAAATATAGAGTTAAACGAAACAAAAAACACAAAAATAAAAATATTAgagg                                                                           >  1:1266328/1‑150 (MQ=255)
                                                                                tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAAAGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAAAATTAAAATTATTCGAGGGGTGa                                                                      >  2:1152169/1‑150 (MQ=255)
                                                                                tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAAAATATGAAAATTATTCGAGGAGTGa                                                                      >  2:361330/1‑150 (MQ=255)
                                                                                tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGa                                                                      >  2:4293508/1‑150 (MQ=255)
                                                                                 gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:650049/150‑1 (MQ=255)
                                                                                 gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:2540868/150‑1 (MQ=255)
                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTGAAAAAATAATGAAATTATTTTTTTTTTAAAATAGAGATAAAGATAAACAAAACGAAAAAATGAAAAATATTCGAGGGGTGTAAg                                                                   >  1:361024/1‑150 (MQ=255)
                                                                                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGTTTTAAAAAAATAAAGAAAATAATTTTTTTTTAATAAATATATAAAGATAAAAAAAAAGAACAAATTAAAAATAATAGATGAGTGGAAGGGaa                                                               >  1:4306682/1‑150 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              >  2:1072931/1‑150 (MQ=255)
                                                                                        acAGCATCTCCTTTGTCGATACCACGAACATTTTCATATCCTCTCTATTGTATACGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:871319/149‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCTATACCACTTAATTTTTCATATCGTGTCTTTTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATATCGATATACCTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:1328329/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:2014808/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:3018543/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:4264125/150‑1 (MQ=255)
                                                                                                tGCTTTGTCTATACCACGTACATTTTCATATCCTGTCTTTTTTTTTCGATTTCAAAATATAATTAAATTTTTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:2360714/150‑1 (MQ=255)
                                                                                                tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGc                                                      >  2:1337461/1‑150 (MQ=255)
                                                                                                    ttGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                  <  2:3074637/150‑1 (MQ=255)
                                                                                                    ttGTCGATACCACGTAATTTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                  <  2:4148852/150‑1 (MQ=255)
                                                                                                         gATACCCCGTAATTTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:19371/150‑1 (MQ=255)
                                                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:2770269/150‑1 (MQ=255)
                                                                                                          aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                            >  2:3995508/1‑150 (MQ=255)
                                                                                                          aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGGTCACAAAAACGACAATATGAAAAATATTAGGGGAGTGGAAGGCCAAAAATCGGCCTCCCgcgag                                            >  2:468864/1‑146 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  2:2408342/150‑1 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  2:4182615/150‑1 (MQ=255)
                                                                                                            cccACGACCATTTCCATCGCCGGGCGTTTTTTTCCTATTTCAAAATATACTGAATTTTTTTTTTTTAAATTATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  1:4086310/149‑1 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAACGCCTCCCGATAGGGa                                         >  2:524697/1‑150 (MQ=255)
                                                                                                               aCGTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATATTGATTTTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  2:1215432/150‑1 (MQ=255)
                                                                                                                  tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAAAATCGAGATAACGATCACAAAAACGAAAAAATGAAAATTATTCGAGGAGTGTAAGGCAAAAATAAGGCCTCCCGAGTGGGAAGCCg                                    >  2:3875218/1‑150 (MQ=255)
                                                                                                                    aaTTTTCATATCCTTTCTTTTTTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:3877473/149‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAAATATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAg                                 >  2:1439750/1‑150 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTGTGTTTTCGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACCATAACAAAAAACACAAAATGTAAATTATTAGAGGAGTGTAAGGCCAAAAAACGGCCTACCGATATGGAAGACGGAg                                 >  2:2952655/1‑150 (MQ=255)
                                                                                                                       tcacATATCCGGCCTTTTGTTTTCTATTTCAAAATATAATTAAATTTTTTGTTTTAAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGttttt                               <  1:3404841/147‑5 (MQ=255)
                                                                                                                         tCATATCCTGTCGTTGTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:3192677/150‑1 (MQ=255)
                                                                                                                          cATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTTTTAGAGGAGTGAAAGGCCAAAAAACGGCCAACCGATAGGGAAGCCGTAGCCAAg                            >  2:2029379/1‑150 (MQ=255)
                                                                                                                               ccTGTCTTTTTTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:1278349/150‑1 (MQ=255)
                                                                                                                               ccTGTCTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATACCTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:2628916/150‑1 (MQ=255)
                                                                                                                                 tGTCTTTTGTTTTCGATTTCAAAATATAATCAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  1:1358872/150‑1 (MQ=255)
                                                                                                                                 tGTAGTTTGTTTTCGATTTCAAAATATAAAGAAATAATTATTTTTTAAATATAGAGATAACTATAAAAAAAACGACAATATGAAAATTATTCTGGGAGTGAAAGGCAAAAAAAAGGCCTCACGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:214918/1‑150 (MQ=255)
                                                                                                                                     gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGCGTCGCGATAGGGAAGCCGTAGCAAAGTGCTCGTGttt                 >  2:204645/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                >  1:290443/1‑150 (MQ=255)
                                                                                                                                       ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               >  1:1529078/1‑150 (MQ=255)
                                                                                                                                           tttCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:700525/150‑1 (MQ=255)
                                                                                                                                                 tttAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGACCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     <  1:6594/150‑1 (MQ=255)
                                                                                                                                                  ttCAAAATATACTGAAATTATTTGTTTTGAAATATCGAGATAACGATCACAAAAACGACAATATGGAAATTCGTCGAGGCGGGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCCAAGTGCGCGGGGTTGTATGCCGGCTGc    >  2:1778533/1‑150 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  1:1179920/1‑150 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  2:299501/150‑1 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  1:2532603/150‑1 (MQ=255)
                                                                                                                                                    cAAAATATACTGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:2263255/150‑1 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGATTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:1059954/150‑1 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:1609110/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:114276/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: