Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 17.4% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC17.4% 257.9 / 5.9 148intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (28/75);  new base C (16/9);  total (52/96)
Fisher's exact test for biased strand distribution p-value = 8.70e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

ACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229029‑2229326
                                                                                                                                                      |                                                                                                                                                        
     tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGggc                                                                                                                                                    >  1:1030792/1‑150 (MQ=255)
      ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                                   <  1:2606975/150‑1 (MQ=255)
       gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                                  <  2:147120/150‑1 (MQ=255)
        tgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGgggggc                                                                                                                                                 >  2:1571500/1‑150 (MQ=255)
         gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGcc                                                                                                                                                <  2:3100198/150‑1 (MQ=255)
         gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGcc                                                                                                                                                <  2:879044/150‑1 (MQ=255)
          aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCa                                                                                                                                               <  1:1503176/150‑1 (MQ=255)
            tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGCGCCCGGGGCGGcccac                                                                                                                                             >  1:3532158/1‑147 (MQ=255)
            tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGgggggggggggcgag                                                                                                                                             >  1:3308449/1‑145 (MQ=255)
            tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGcccaa                                                                                                                                             >  2:2995193/1‑147 (MQ=255)
            tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAt                                                                                                                                             >  1:941069/1‑150 (MQ=255)
                gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                         <  1:1653182/150‑1 (MQ=255)
                   tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGCGCGGGGGGCGGCCCCAcgccgcc                                                                                                                                      >  1:3540306/1‑150 (MQ=255)
                   tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGGGGGCCAACcgcgcc                                                                                                                                       >  2:4172775/1‑147 (MQ=255)
                     tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccgc                                                                                                                                    <  1:4103283/150‑1 (MQ=255)
                     tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccgc                                                                                                                                    <  1:3941637/150‑1 (MQ=255)
                       aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCAAAAGCcccccccc                                                                                                                                  >  2:2416831/1‑150 (MQ=255)
                       aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATAGCCCCCGccc                                                                                                                                  >  2:2417561/1‑150 (MQ=255)
                       aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGccc                                                                                                                                  <  2:1296906/150‑1 (MQ=255)
                          tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCAAAGCccccccccccc                                                                                                                               >  2:4076834/1‑149 (MQ=255)
                          tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGACGGGGGGGGCAATAGCCGccccccccc                                                                                                                               >  1:3851388/1‑149 (MQ=255)
                              atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAAGCCGcccccccccccac                                                                                                                           >  2:568811/1‑145 (MQ=38)
                               ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGGCCAAACccccccccccccccccc                                                                                                                           >  2:865035/1‑145 (MQ=255)
                                 ttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCAGGGGCGGCCCATAGCCGCCCCCCCCCGcccccc                                                                                                                        >  2:2580036/1‑150 (MQ=38)
                                     aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                    <  2:1945919/150‑1 (MQ=255)
                                     aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTAAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGATTCATATAATCACTGGAGCAAGGAGCGCGCCTGGAGAGGGCAATGGCCGCCCACCCCCGCACACCCcgg                                                                                                                    >  1:2571271/1‑150 (MQ=255)
                                           ttaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGCGCCAAACGCCCCCCCCCCCAGCAACCCCCGGCACTgg                                                                                                              >  1:2447014/1‑150 (MQ=255)
                                              atGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGa                                                                                                          <  2:1029045/150‑1 (MQ=255)
                                                   atcaGGACCTAAAATAACGATAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGATTAATATAATCACTGGCGAATGGAGCGCGCCGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaa                                                                                                    <  1:2389808/150‑1 (MQ=25)
                                                    tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCAAAACCCCCCGCCCCCACCACCCCCCCGCGCTGGCGGACaaaa                                                                                                   >  1:2168875/1‑150 (MQ=25)
                                                    tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCAAAGCCCCCCCCCCCCGCCCCCCCCCGCGCGGGCGCACaaaa                                                                                                   >  2:4178616/1‑150 (MQ=25)
                                                    tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACaaaa                                                                                                   >  2:2840801/1‑150 (MQ=255)
                                                      aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGGCCCACCCCCCCCCCCCCCCCCCCCCCCGCCCCGGGCGAaaaaaaaa                                                                                                 >  2:564815/1‑148 (MQ=255)
                                                      aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATc                                                                                                 >  2:2877097/1‑150 (MQ=255)
                                                      aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCCGGCCCCCCCCGGCGCGGGCGCACAAAATc                                                                                                 >  2:2479207/1‑150 (MQ=25)
                                                        gTCCGAAAATACCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                               <  2:1238936/150‑1 (MQ=255)
                                                         tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                              <  2:3922399/150‑1 (MQ=255)
                                                          ccGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATcgccg                                                                                             >  2:2915174/1‑150 (MQ=255)
                                                          ccGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAACCCCCCCCCCCCAGCCCCCCCCCGCGCAGGCGCACacaaaacccc                                                                                             >  2:4214856/1‑144 (MQ=25)
                                                                aTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc                                                                                       <  2:2253379/150‑1 (MQ=255)
                                                                aTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc                                                                                       <  2:2253353/150‑1 (MQ=255)
                                                                   aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCCCACAGCGCCGGCCCCCGCCCCCCCCCCGGCGGGGGGCCAAAAATCGCCGCTGCGCgg                                                                                     >  2:1858132/1‑150 (MQ=255)
                                                                      agaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                                 <  2:2299499/150‑1 (MQ=255)
                                                                                  tcgctctTCCTGTTGCGCTCCTGTCATGGGAATTGCTACATATAACCCCTGGCGCACGGCGCGCGCGGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                     <  1:1710032/147‑1 (MQ=14)
                                                                                    tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATCCCCCCCCCCCCCACCACCCCCCGGCTCAGGCCAACAAAAAACCCCCAGCGCGGTGCCCACCGCTTATacca                                                                   >  2:3588962/1‑149 (MQ=18)
                                                                                      tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATACCCGCCCCCCCCACCAACCCCCGGCTCATGCGAAAAAAAAACCCCCAGCCCGGTGCCCACCGCAGATCCCATa                                                                 >  2:2133803/1‑150 (MQ=12)
                                                                                      tcACCCTGTGGCGCTCCTGACATGTGCTTTGCTTCATATAATCACTGGCGCACGGCGCGCGCCGGGGGCGCCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                 <  1:1428158/150‑1 (MQ=17)
                                                                                      ccATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                 <  1:2555703/149‑1 (MQ=255)
                                                                                        aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACg                                                               <  2:1344555/150‑1 (MQ=255)
                                                                                            tgtgGCGCTCGTGTCTTGTGCTTTGCTTCATATAATCAGTGGCGCATGGTGCGCGCCGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTa                                                          <  1:934745/150‑1 (MQ=17)
                                                                                            tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTa                                                          <  1:3147106/150‑1 (MQ=255)
                                                                                            tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTa                                                          <  2:2601029/150‑1 (MQ=255)
                                                                                            tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACGGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTa                                                          <  2:2954871/150‑1 (MQ=255)
                                                                                                 ggcTCCTGTCTTGTGCATTGCTTCATATAATCCCTGGCGCACGGTGCGGGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCggg                                                     <  1:776710/149‑1 (MQ=17)
                                                                                                 cgcTCCTGTCTTGTGCTTTGCTTCATATAATCACTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCggg                                                     <  1:776623/150‑1 (MQ=255)
                                                                                                   cTCCTGTCTGGTGCTTTGCTTCATATAATCAGTGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  1:1853015/150‑1 (MQ=17)
                                                                                                   cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  1:3469719/150‑1 (MQ=255)
                                                                                                    tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGCCAAAAGCCGCCCCCCCCCGCACCCCCCGGCTCGGGCCGAAAAAAAACCCCCAGCGCGGTGCCCACCGCGTATACCATACGGCTAACCgggcg                                                   >  2:1752358/1‑148 (MQ=12)
                                                                                                    tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAAACGCCCCGGCGCGGTGCCCTCCGCGTATCCCATACGGCTACCCGGTCg                                                  >  2:2912175/1‑150 (MQ=17)
                                                                                                    tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAAAAAAATAGCCGCAGCGCGGTGCCCACGGCTTATCCCATAAGGCTAACCGGTCg                                                  >  2:735810/1‑150 (MQ=17)
                                                                                                     ccTGTCTTGTGCTTTGCTTAATATAATCACTGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  1:1906497/150‑1 (MQ=14)
                                                                                                     ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCTCCGCTGCGCGGTGCCCTCGGATTATCCCTTACGGCTACCGGGTCgg                                                 <  2:2365088/150‑1 (MQ=255)
                                                                                                     ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  2:431194/150‑1 (MQ=255)
                                                                                                      cTGTCTTGTGCATTGCTTAATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  1:3033713/150‑1 (MQ=255)
                                                                                                      cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  2:3258075/150‑1 (MQ=255)
                                                                                                      cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  2:3532158/150‑1 (MQ=255)
                                                                                                      cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  2:941069/150‑1 (MQ=255)
                                                                                                      cTGTCATGTGCATTGCTTCATATAATCACGGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                <  2:3428879/150‑1 (MQ=255)
                                                                                                         tCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATCGCCCCCGCCCCCAGCACCCCCCGGCGCTGGCCAACAAAATCCCCGCAGCCCGGGGCCCACGGCGTATACCTTACCGCTACCCGGTCGGgcgc                                             >  2:1041871/1‑150 (MQ=17)
                                                                                                         tCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAAGCCCCCCCCCCCAGCACCCCCCGGCGCTGGCGGACAAAAACGCCCCGGCGCGGTGGCCACCGCTTATCCCGTAAGGCGAACCGGTCGGgcgc                                             >  2:357048/1‑150 (MQ=12)
                                                                                                          cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                            <  1:345378/150‑1 (MQ=255)
                                                                                                           aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  2:3055392/150‑1 (MQ=255)
                                                                                                            tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  1:2417561/150‑1 (MQ=255)
                                                                                                            tgtgCATTGCTTCATATAATCACGGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  1:4178616/150‑1 (MQ=255)
                                                                                                                  ttGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCATTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                    <  2:4434/150‑1 (MQ=255)
                                                                                                                    gCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                  <  2:2830478/150‑1 (MQ=255)
                                                                                                                      ttCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                <  2:3443525/150‑1 (MQ=40)
                                                                                                                       tCATATACTCCCTGGCGCACGGAGCGGGCCGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  1:4201573/150‑1 (MQ=11)
                                                                                                                         atatAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                             <  2:3366036/150‑1 (MQ=38)
                                                                                                                         atatAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                             <  2:3366098/150‑1 (MQ=38)
                                                                                                                             aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  1:3974748/150‑1 (MQ=34)
                                                                                                                                cACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                      <  2:4207488/150‑1 (MQ=32)
                                                                                                                                 aCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  2:3130511/150‑1 (MQ=32)
                                                                                                                                  cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  2:3167296/150‑1 (MQ=32)
                                                                                                                                  cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  2:2571913/150‑1 (MQ=32)
                                                                                                                                  cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:3994032/150‑1 (MQ=32)
                                                                                                                                       gcAAGGAGCGCGCAGGGGGCGGCCAATCCCCCCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATAGCCGCGGCGCGGTGCCCTCGGCGTATCCCATACCGCTAACCGGTCGGGCGCGAGGTAACAACCCAGTAAAACCCGCCCGCa               >  2:3960275/1‑150 (MQ=11)
                                                                                                                                        cAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg              <  1:2627440/150‑1 (MQ=32)
                                                                                                                                        cAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg              <  2:3499643/150‑1 (MQ=32)
                                                                                                                                          aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  2:1455886/150‑1 (MQ=32)
                                                                                                                                          aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  1:4214856/150‑1 (MQ=32)
                                                                                                                                            gAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca          <  1:1201089/150‑1 (MQ=32)
                                                                                                                                               cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcacac       <  1:480486/150‑2 (MQ=17)
                                                                                                                                                gcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc      >  2:663986/1‑150 (MQ=32)
                                                                                                                                                gcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc      >  2:1977749/1‑150 (MQ=32)
                                                                                                                                                gcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc      >  2:2719489/1‑150 (MQ=32)
                                                                                                                                                 cgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc     <  1:3537400/150‑1 (MQ=32)
                                                                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  <  2:3989722/150‑1 (MQ=32)
                                                                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  <  1:3497083/150‑1 (MQ=32)
                                                                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  <  2:3989600/150‑1 (MQ=32)
                                                                                                                                                      |                                                                                                                                                        
ACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229029‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: