Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,503 T→A 5.2% intergenic (‑129/‑144) glpT ← / → glpA sn‑glycerol‑3‑phosphate transporter/anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,5030TA 5.2% 677.2 / 6.7 201intergenic (‑129/‑144)glpT/glpAsn‑glycerol‑3‑phosphate transporter/anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (97/87);  new base A (14/2);  total (112/89)
Fisher's exact test for biased strand distribution p-value = 7.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAA  >  NC_000913/2352354‑2352652
                                                                                                                                                     |                                                                                                                                                     
tGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACat                                                                                                                                                       >  2:3554911/1‑150 (MQ=255)
 ggTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACatt                                                                                                                                                      <  1:3564449/150‑1 (MQ=255)
 ggTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACatt                                                                                                                                                      <  1:1467881/150‑1 (MQ=255)
  gTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACatta                                                                                                                                                     >  1:3423178/1‑150 (MQ=255)
     tAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACAttatta                                                                                                                                                  <  2:496134/150‑1 (MQ=255)
     tAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACAttatta                                                                                                                                                  <  2:4154278/150‑1 (MQ=255)
      aaaaaTACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTaa                                                                                                                                                 <  2:3341460/150‑1 (MQ=255)
       aaaaTACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGGCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTaaa                                                                                                                                                <  2:3252915/150‑1 (MQ=255)
        aaaTACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAAt                                                                                                                                               >  2:2743989/1‑150 (MQ=255)
             cTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACCTTATTAAATAAGAt                                                                                                                                          >  1:3007099/1‑150 (MQ=255)
             cTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGAt                                                                                                                                          >  1:3988077/1‑150 (MQ=255)
                 accttGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTAc                                                                                                                                      <  2:1848692/147‑1 (MQ=255)
                 aCATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTAc                                                                                                                                      <  2:3875622/150‑1 (MQ=255)
                        tAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGt                                                                                                                               >  2:3463811/1‑150 (MQ=255)
                        tAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTGTCGTGAGTTGCCGCGATGTTAAGAAAACAGTCCTACATTAAAGGTGAAGTGCCGCACCCATTATTAAATAAGATTTACACACTGt                                                                                                                               >  2:3739065/1‑150 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCa                                                                                                                            >  1:2038174/1‑150 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCa                                                                                                                            >  2:2784784/1‑150 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCa                                                                                                                            >  2:2774006/1‑150 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCa                                                                                                                            >  1:2788003/1‑150 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCa                                                                                                                            <  1:857285/150‑1 (MQ=255)
                           ccTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCATTAGTTGCCGCGATGTTAAGAAAAAATTAATAAATTAAATGTGAATTTCCGCACACATTATTAAATAAGATTTACAAAATTTTCa                                                                                                                            >  1:4186207/1‑150 (MQ=255)
                                 tGGCCCGTTGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTAATAAATTTAATGTGAATTGCCGCACACAATATTAAATAAGATTTACAAAATGTTCAAAATGa                                                                                                                      <  2:4193666/150‑1 (MQ=255)
                                 tGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACAATATTAAATAAGATTTACAAAATGTTCAAAATGa                                                                                                                      <  2:2051665/150‑1 (MQ=255)
                                  ggCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGAc                                                                                                                     <  1:388556/150‑1 (MQ=255)
                                      cGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCAt                                                                                                                 >  2:1102427/1‑150 (MQ=255)
                                      cGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCAt                                                                                                                 <  2:195289/150‑1 (MQ=255)
                                      cGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCAt                                                                                                                 <  2:247044/150‑1 (MQ=255)
                                        tGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTTCCGCGATGTTAAGAAAACAATAATAAATTAAATGTGAATTGACGCACACAATATTAAATAAGATTTAAAAAATGTTCAAAATTAAGCATGa                                                                                                               >  1:1113083/1‑150 (MQ=255)
                                         ggTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGaa                                                                                                              >  2:262802/1‑150 (MQ=255)
                                           tCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTTCCGCGATGTTAAGAAAACAATCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTAAAAAATGTTCAAAATGACGCATGaaaa                                                                                                            >  1:1684597/1‑149 (MQ=255)
                                           tCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAAt                                                                                                            >  2:3444177/1‑150 (MQ=255)
                                           tCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAAAATTCATAAATTAAATGTGAATTTCCGCACACAATATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAAt                                                                                                            >  1:2390465/1‑150 (MQ=255)
                                            cTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATc                                                                                                           >  2:4034289/1‑150 (MQ=255)
                                            cTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATc                                                                                                           <  2:390689/150‑1 (MQ=255)
                                             ttatttatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCa                                                                                                          <  2:383786/150‑1 (MQ=255)
                                             ttatttatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCa                                                                                                          <  1:226207/150‑1 (MQ=255)
                                             ttatttatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCa                                                                                                          <  1:452621/150‑1 (MQ=255)
                                                tttatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTGTCTTGAGTTTCCGCGATGTTAAGAAAACATTAATAAATTAAATTTGTATTGCCGCACACATTATTAAATAAGATTTACAAAAAGGTCAAAATGACGCATGAAATCACGt                                                                                                       >  1:1145855/1‑150 (MQ=255)
                                                tttatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTGTCTTGAGTTGCCGCGATGTTAAGAAAACATTCAAAAATTAAATGTGAATTTCCGCACACATTATTAAATAATATTTACAAAAATTTCAAAATGAAGCATGAAATCACGt                                                                                                       >  1:2137733/1‑150 (MQ=255)
                                                  tatGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGGTAAGAAAACATTAATAAATTAAATGTGAATTTCCGCACACAATATTAAATAAGATTTACAAAATGTTAAAAATTACGCATGAAATCAccgtt                                                                                                     >  1:2075524/1‑146 (MQ=255)
                                                    tGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTTCCGCACACCATATTAAATAAGATTTTCAAAATGTTCAAAATGACGCATGAAATCACGTTTCa                                                                                                   >  1:771337/1‑150 (MQ=255)
                                                     gATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCAc                                                                                                  <  1:2821701/150‑1 (MQ=255)
                                                        tAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACttt                                                                                               <  2:1432471/150‑1 (MQ=255)
                                                         aaCAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTc                                                                                              <  2:199042/150‑1 (MQ=255)
                                                          aCAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTTCCCCGATGTTAAGAAAACAATCATAAATTAAAATAGAAATGCACCACACATAATTAAATAAGATTTACAAAAAGATCAAAAAGACGCAATAAAACAAGTTTCAAtttag                                                                                             >  1:2837963/1‑148 (MQ=255)
                                                            aGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGaa                                                                                           <  2:1181388/150‑1 (MQ=255)
                                                              ccTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTTCCGCGATGTTAAGAAAAAATTAATAAATTAAATGTGAATTGCCGCACACAATATTAAATAAGATTTAAAAAATGTTAAAAATTACGCATTAAATCACGTTTCACTTTCGAAtt                                                                                         >  1:2726889/1‑150 (MQ=255)
                                                              ccTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAAtt                                                                                         <  2:2817979/150‑1 (MQ=255)
                                                                    tCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGc                                                                                   <  2:2950104/150‑1 (MQ=255)
                                                                    tCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGc                                                                                   <  2:2224471/150‑1 (MQ=255)
                                                                        tGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATTTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAat                                                                               >  2:1503496/1‑150 (MQ=255)
                                                                           gagaACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTTCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATTACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATg                                                                            >  2:1844910/1‑150 (MQ=255)
                                                                           gagaACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATg                                                                            >  2:878043/1‑150 (MQ=255)
                                                                                   gCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAAt                                                                    >  2:2901119/1‑150 (MQ=255)
                                                                                    ccGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATc                                                                   >  2:2982041/1‑150 (MQ=255)
                                                                                       tttCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCaaa                                                                <  1:2659521/150‑1 (MQ=255)
                                                                                       tttCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCaaa                                                                <  1:1728472/150‑1 (MQ=255)
                                                                                                ttGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATg                                                       >  2:4101490/1‑150 (MQ=255)
                                                                                                    cgcgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGtttt                                                   >  1:619059/1‑150 (MQ=255)
                                                                                                    cgcgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGtttt                                                   >  1:619096/1‑150 (MQ=255)
                                                                                                     gcgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGttttt                                                  >  2:1688286/1‑150 (MQ=255)
                                                                                                     gcgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGttttt                                                  <  1:4218159/150‑1 (MQ=255)
                                                                                                      cgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTa                                                 >  2:1490121/1‑150 (MQ=255)
                                                                                                      cgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTa                                                 <  1:54719/150‑1 (MQ=255)
                                                                                                        aTGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACt                                               <  1:895676/150‑1 (MQ=255)
                                                                                                         cgctAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCGAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTa                                              <  2:1301958/147‑1 (MQ=255)
                                                                                                           ttAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATg                                            >  1:1002071/1‑150 (MQ=255)
                                                                                                           ttAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATg                                            >  1:2772978/1‑150 (MQ=255)
                                                                                                           ttAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATg                                            >  1:3451534/1‑150 (MQ=255)
                                                                                                            tAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATgg                                           <  1:773505/150‑1 (MQ=255)
                                                                                                             aaGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAACTGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGc                                          <  2:552107/150‑1 (MQ=255)
                                                                                                             aaGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGc                                          <  1:4064262/150‑1 (MQ=255)
                                                                                                             aaGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGc                                          <  1:3058531/150‑1 (MQ=255)
                                                                                                             aaGAAAACATTAATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGc                                          <  2:3043987/150‑1 (MQ=255)
                                                                                                                   aCATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATg                                    <  2:2299921/150‑1 (MQ=255)
                                                                                                                      ttCATAACTTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTa                                 <  1:1208282/150‑1 (MQ=255)
                                                                                                                         aTAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTaaaa                              >  1:2559076/1‑150 (MQ=255)
                                                                                                                            aattaaatGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAAc                           >  1:3747678/1‑150 (MQ=255)
                                                                                                                             attaaatGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACg                          >  1:3489896/1‑150 (MQ=255)
                                                                                                                             attaaatGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACg                          <  2:793197/150‑1 (MQ=255)
                                                                                                                                  atGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACtt                     >  1:2713626/1‑150 (MQ=255)
                                                                                                                                     tgAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCag                  >  1:2217840/1‑150 (MQ=255)
                                                                                                                                      gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                 <  1:4145435/150‑1 (MQ=255)
                                                                                                                                      gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                 <  2:2446617/150‑1 (MQ=255)
                                                                                                                                      gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                 <  1:2532404/150‑1 (MQ=255)
                                                                                                                                      gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                 <  1:3119144/150‑1 (MQ=255)
                                                                                                                                      gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCAAGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                 >  1:2027197/1‑150 (MQ=255)
                                                                                                                                       aaTTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCagag                >  2:317169/1‑150 (MQ=255)
                                                                                                                                            ccGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATa           >  1:402451/1‑150 (MQ=255)
                                                                                                                                            ccGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATa           >  2:2016049/1‑150 (MQ=255)
                                                                                                                                             cGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATaa          <  1:3417416/150‑1 (MQ=255)
                                                                                                                                             cGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATaa          <  1:2447610/150‑1 (MQ=255)
                                                                                                                                               cacacaTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACa        >  2:4134291/1‑150 (MQ=255)
                                                                                                                                               cacacaTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACa        >  2:3676490/1‑150 (MQ=255)
                                                                                                                                               cacacaTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATCGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACa        >  2:739860/1‑150 (MQ=255)
                                                                                                                                                   caTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGa    >  1:1935948/1‑150 (MQ=255)
                                                                                                                                                   caTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGa    >  2:1575487/1‑150 (MQ=255)
                                                                                                                                                    attattAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa   <  1:1503496/150‑1 (MQ=255)
                                                                                                                                                    attattAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa   >  2:139290/1‑150 (MQ=255)
                                                                                                                                                    attattAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa   >  2:954041/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAA  >  NC_000913/2352354‑2352652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: