Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,596 G→C 12.0% intergenic (‑127/+54) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,5960GC12.0% 503.7 / 11.3 168intergenic (‑127/+54)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base G (95/43);  new base C (2/19);  total (97/71)
Fisher's exact test for biased strand distribution p-value = 2.50e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

ACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAG  >  NC_000913/2460447‑2460744
                                                                                                                                                      |                                                                                                                                                    
aCCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAagag                                                                                                                                                      >  1:1306448/1‑150 (MQ=255)
 ccAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAgaga                                                                                                                                                     >  1:1976503/1‑150 (MQ=255)
    gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                                                                  <  2:247708/150‑1 (MQ=255)
    gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                                                                  >  2:2525731/1‑150 (MQ=255)
    gCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaa                                                                                                                                                  <  2:41577/150‑1 (MQ=255)
      ggTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaaga                                                                                                                                                >  1:633892/1‑150 (MQ=255)
         aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                                                             <  2:426939/150‑1 (MQ=255)
         aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                                                             <  2:2518012/150‑1 (MQ=255)
         aaaaCCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgaaaagaaaa                                                                                                                                             <  2:2473661/150‑1 (MQ=255)
              cTGACCCATAACACTCCCCTTTAAAAAAATAAATAAAGTGGTCTGACCTGATCATTGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGccc                                                                                                                                        <  2:2187955/150‑1 (MQ=255)
              cTGACCAATAACACTCCCCTGTAAAAAAATATATAATGTGGTCTGACCTGATCATAGTCTTAACAATTTTTTT‑ACTTTTAGCCAAGTGTAGAAAAGGGAAAGTGGGAGATTTGACACAGAGAGAAAATAAGAAGAGAAAAGAAAACGccc                                                                                                                                        <  2:225612/150‑1 (MQ=255)
               tGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGcccc                                                                                                                                       >  1:848138/1‑150 (MQ=255)
                 aCCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTg                                                                                                                                     >  1:2155050/1‑150 (MQ=255)
                 aCCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTg                                                                                                                                     >  2:701406/1‑150 (MQ=255)
                    cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                                                                  >  2:1643045/1‑150 (MQ=255)
                         cacTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGTAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAAc                                                                                                                             >  1:3158588/1‑150 (MQ=255)
                            tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                          >  1:431537/1‑150 (MQ=255)
                            tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                          >  1:728496/1‑150 (MQ=255)
                            tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                          >  2:2837218/1‑150 (MQ=255)
                            tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                          >  2:3119453/1‑150 (MQ=255)
                            tCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                          >  2:678613/1‑150 (MQ=255)
                               ccTTTAAAAAAATAAATATTGTGGTCTGAACTGATCTTATTCTAAAACATTTTTTT‑ACATTTAGCCAAGTGGATATAAGTTAAAGTGGGTGATATGACACAGAGAGAAAAGAATAACAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                                                                       <  2:3419775/150‑1 (MQ=255)
                                 tttAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGTAGAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAggg                                                                                                                     <  2:2062947/148‑1 (MQ=255)
                                 tGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAggg                                                                                                                     >  2:1782310/1‑150 (MQ=255)
                                  gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTCT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                    >  2:211322/1‑150 (MQ=255)
                                   tAAAAAAATAAATAACGTGGTCTGACCTGATAATTGTCTTAACATTTTTTTT‑ACATTTAGCCAATTGGAGAAATGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                   <  2:912794/150‑1 (MQ=255)
                                     aaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgt                                                                                                                 >  1:3193686/1‑150 (MQ=255)
                                            aattaaaGTGGTCTGACGTGATCATTGTCTTAACCTTTTTTTT‑ACTTTTAGCCAAGTGTTGAAAAGGGAAAGTGGTAGCTATGACACAGAGAGAAAAGAAGAATAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                          <  2:586513/147‑1 (MQ=255)
                                             aatctcgTGGTCTGACCTGATCTTAGTCTTAACCATTTTTTT‑ACATTTAGCCACGTGGAGAAAAGGGAAAGTGGGAGCTATGAAACCGAGAGAAAAGAAGAAGAGAAACCAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAccc                                                                                                         <  2:1707947/144‑1 (MQ=255)
                                                 caGTGGTCTGACCGTATCATTGTCTTAACCTTTTTTTT‑ACATTTAGCCACGTGGAGAAAAGGTAAAGTGGGAGCTAGGACACAGAGAGAAAAGAAGAATATAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                                                                     <  2:3166877/149‑1 (MQ=255)
                                                    tGGTCTGCCCTGATCATCGTATTAACCATGTTTTT‑ACTTTTAGCCAAGTGGAGAACAGGGAAAGTGGGCGCTATGACACAGAGAGAAAACAAGAAGAGAAACGAAAGCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGa                                                                                                  <  2:1225792/150‑1 (MQ=255)
                                                       tCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGACtt                                                                                               >  2:2504611/1‑150 (MQ=255)
                                                       tCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                               >  1:2766976/1‑150 (MQ=255)
                                                       tCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                               >  2:2646930/1‑150 (MQ=255)
                                                             cttaTCATAGTATTAACAATTTTTTT‑AAATTTAGCCCAGTGGATAAATGGGAATGTGGGATCTTTGACACAGATATAAAATAAGACGACAAACCAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                         <  1:3545485/147‑1 (MQ=255)
                                                             cTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                         >  2:1539665/1‑150 (MQ=255)
                                                               gATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGATAAAAGGGAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                                                       <  2:3953433/150‑1 (MQ=255)
                                                               gATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                                                       >  1:2278125/1‑150 (MQ=255)
                                                                      attctAAACATTTTTTTAAAATTTCGCCATGTGTATAAATGGTATTGTGGTAGATATCACACACACACAAAATAACAACAAAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGc                                                                                 <  1:418928/146‑1 (MQ=255)
                                                                       tCTTAACCATTTTTTT‑ACATTTAGCCCAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGACCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                               >  2:2102804/1‑150 (MQ=255)
                                                                       tCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGACTTAACGCAGACCCAGCTg                                                                               >  2:1969292/1‑150 (MQ=255)
                                                                       tCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                               >  2:640433/1‑150 (MQ=255)
                                                                       tCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                               >  2:2325403/1‑150 (MQ=255)
                                                                       ccTTAACTATTTTTTT‑ACTTTTCGCCATGTGTACAAATTGTATGGTGGTTGCTATTAAACAGATATAAAAGAATAACATAAACTAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTg                                                                               <  1:3737350/149‑1 (MQ=255)
                                                                         ttAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAACCGGGAAAGTGGGTGCTATGACACAGCGAGAAATGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGa                                                                             <  2:2119004/150‑1 (MQ=255)
                                                                               aTTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                                                       >  1:3142687/1‑150 (MQ=255)
                                                                               aTTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                                                       >  1:3958239/1‑150 (MQ=255)
                                                                                cttttta‑ACATAAAGCCAACTGCACAAAAGGGAAACTGGGACCTAGCACCCACAGACAAAACAACACCACAAAACAACACCCCCCTGCCAGCAACTCACAGGGGCGTACGTACCCGAGACGAAATAACGCAGACCCAGCTCGAAAATCAg                                                                      <  2:1030858/149‑1 (MQ=255)
                                                                                      t‑ACATTTAGCCAAGTGGAGAAAAGGTAAAGTGGGAGCTATGACACTGAGAGAAAAGACGAAGAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                <  1:2546577/150‑1 (MQ=255)
                                                                                      t‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                <  2:2563538/150‑1 (MQ=255)
                                                                                           tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                            >  2:636975/1‑150 (MQ=255)
                                                                                           tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                            >  1:1057623/1‑150 (MQ=255)
                                                                                           tttAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCt                                                            >  1:501723/1‑150 (MQ=255)
                                                                                               gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                        >  1:819274/1‑150 (MQ=255)
                                                                                                ccATGTGGATAAACGGTAATGTGGGAGATATGACACAGAGAGAAAAGAAGAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                       <  2:533074/150‑1 (MQ=255)
                                                                                                ccACGTGTAGAAAAGGGAAAGTGGGAGATATGACACAGAGAGAAAATAAGAACAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                       <  1:3110752/150‑1 (MQ=255)
                                                                                                ccAAGTGTACAAAAGGCAAATTGGCAGCAATCAAACATACACAAAATAACAAGACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                       <  1:1422388/150‑1 (MQ=255)
                                                                                                 cAAGTGGATAAAAGGGAATGTGGGATCTATGACACAGAGAGAAAAGAATAAGACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAg                                                      <  1:3785034/150‑1 (MQ=255)
                                                                                                  aaGTGTATAAACGGGAATGTGGTAGATATGACACAGAGACAAAAGAAGAAGACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                     <  1:556395/150‑1 (MQ=255)
                                                                                                  aaGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAgg                                                     >  2:587768/1‑150 (MQ=255)
                                                                                                         gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                                              >  2:3475217/1‑150 (MQ=255)
                                                                                                             cgttaaaGTGGGAGATTTGACACATAGATAAAAGAATAAGTTAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGATGAATTAACGCAGACCCAGCTGGAAAATCATCATTTCAGCTTCGCAGGCATATGTGaaa                                          <  1:3492282/146‑1 (MQ=255)
                                                                                                             aGGTAAAGTGGTAGCTATGACACTGATATAAAAGAAGAAGAGAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa                                          <  2:396627/150‑1 (MQ=255)
                                                                                                             aGGGAAAGTGGGAGCTATGACCCAGAGCGACACGAAGACGAGCAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGaaa                                          <  2:3955118/150‑1 (MQ=255)
                                                                                                               ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGACATc                                        >  2:241632/1‑150 (MQ=255)
                                                                                                               ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc                                        >  1:1467172/1‑150 (MQ=255)
                                                                                                               ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc                                        >  1:4200452/1‑150 (MQ=255)
                                                                                                               ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc                                        >  2:816205/1‑150 (MQ=255)
                                                                                                                taaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg                                       <  2:2162139/149‑1 (MQ=255)
                                                                                                                 aaaGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                      >  2:2912178/1‑150 (MQ=255)
                                                                                                                  tggTGGGAGCTATGACACATAGACAAAACAAGAAGACAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat                                     <  2:1859954/148‑1 (MQ=255)
                                                                                                                  aaGTGGCATCTATCACACATATATAAAAGAAGAACAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat                                     <  1:48060/150‑1 (MQ=255)
                                                                                                                   aGTGGGAGCAAAGACACACACACAAAACAACAACACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata                                    <  1:3067358/150‑1 (MQ=255)
                                                                                                                    gTGGGAGCTATGACACAGAGATAAAAGAAGACGACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                   <  2:118469/150‑1 (MQ=255)
                                                                                                                    gTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGatat                                   >  1:2206173/1‑150 (MQ=255)
                                                                                                                       ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCa                                >  2:818662/1‑150 (MQ=255)
                                                                                                                       ggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCa                                >  1:2577907/1‑150 (MQ=255)
                                                                                                                        gAGCTATGACACAGAGCTAAAACAACCACACAAACGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAg                               <  1:1575197/150‑1 (MQ=255)
                                                                                                                        gAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAg                               >  2:2285782/1‑150 (MQ=255)
                                                                                                                         aGCTATGACACCGAGAGAAAACAAGAAGAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAgg                              <  2:1135130/150‑1 (MQ=255)
                                                                                                                          gCTATGACACAGAGATAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgc                             <  1:680166/150‑1 (MQ=255)
                                                                                                                             aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                          >  1:420887/1‑150 (MQ=255)
                                                                                                                                  acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGACATCGATATCCAGGCGCACACCg                     >  1:1941271/1‑150 (MQ=255)
                                                                                                                                     gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                  >  1:3748497/1‑150 (MQ=255)
                                                                                                                                     gagacaAAATAATAAGATAACCCAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                  <  2:3641325/150‑1 (MQ=255)
                                                                                                                                       gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                <  1:3530888/150‑1 (MQ=255)
                                                                                                                                       gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                <  1:2540872/150‑1 (MQ=255)
                                                                                                                                        agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc               >  1:3400512/1‑150 (MQ=255)
                                                                                                                                        agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc               >  1:178020/1‑150 (MQ=255)
                                                                                                                                          aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGActc             >  1:3702766/1‑150 (MQ=255)
                                                                                                                                            aagaagaagAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCtt           <  2:3452494/150‑1 (MQ=255)
                                                                                                                                            aagaagaagACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCtt           <  2:1670670/150‑1 (MQ=255)
                                                                                                                                                    gagaAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   <  1:2911625/150‑1 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   <  1:272379/150‑1 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   <  2:2460051/150‑1 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   >  1:2843941/1‑150 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   >  1:3006001/1‑150 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   >  1:3991111/1‑150 (MQ=255)
                                                                                                                                                    gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa   <  2:3867045/150‑1 (MQ=255)
                                                                                                                                                     agaaaagaaaACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg  >  2:4323245/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ACCAGCGGTAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTT‑ACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAG  >  NC_000913/2460447‑2460744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑