Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 19.2% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA19.2% 515.7 / 32.1 209Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (54/108);  new base A (45/2);  total (99/110)
Fisher's exact test for biased strand distribution p-value = 1.50e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

TAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGC  >  NC_000913/2470281‑2470575
                                                                                                                                                  |                                                                                                                                                    
  gtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAAAAGTCGTTTTTTTAATTTATAAAAAAATTCaaata                                                                                                                                                 >  2:149718/1‑148 (MQ=255)
   taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTataaaataaataaaataa                                                                                                                                                >  1:161475/1‑150 (MQ=255)
      ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                             >  1:3193511/1‑150 (MQ=255)
      ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                             <  2:2735685/150‑1 (MQ=255)
        ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                           <  1:2097468/150‑1 (MQ=255)
         tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAtaaataaat                                                                                                                                          >  1:2793568/1‑149 (MQ=255)
          ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                         <  2:372399/150‑1 (MQ=255)
              ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGACAAt                                                                                                                                     >  2:1752002/1‑150 (MQ=255)
                 atatCTCAATTTTATGTGTCATTTTTCGTGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                  <  2:3660932/150‑1 (MQ=255)
                 atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAAAAATAAAAAAAAAAGc                                                                                                                                  >  1:1291239/1‑150 (MQ=255)
                        aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAATACAATATCGCTGTaa                                                                                                                           >  1:2832300/1‑150 (MQ=255)
                        aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATAAAATATAGATGTaa                                                                                                                           >  2:3161350/1‑150 (MQ=255)
                          ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                         <  2:3476705/150‑1 (MQ=255)
                            ttttgtgTCATTTTTCGGGGAAGGATTTAGAGATTGAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                       <  1:3731522/147‑1 (MQ=255)
                            ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                       <  1:2597339/150‑1 (MQ=255)
                             tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                                                                                                      <  2:1174180/150‑1 (MQ=255)
                              aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTTAAATAATTAAAATAAATGAATACAATAGGGTTGTAAAGCagt                                                                                                                     >  1:1052337/1‑148 (MQ=255)
                                 tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAATAAATAAAAAAAAAAGAGATGTAAACCaaaaag                                                                                                                  >  1:1165767/1‑145 (MQ=255)
                                  gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                 <  2:1324931/150‑1 (MQ=255)
                                  gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                 <  2:1056663/150‑1 (MQ=255)
                                   tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                <  2:729100/150‑1 (MQ=255)
                                    cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACCAGAGCGATGTAACGCACGAAGttt                                                                                                               >  1:2865593/1‑150 (MQ=255)
                                     aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAAAAAAAACAAAAGCCATGTAAAGCACAAAATTTa                                                                                                              >  2:1225960/1‑150 (MQ=255)
                                       ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                            <  1:4165599/150‑1 (MQ=255)
                                        tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAATAAAAAAAAAAGAGATGTAAAGAACAAAGTTTAAAt                                                                                                           >  2:1960198/1‑150 (MQ=255)
                                        tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAATAAAACAATACAAAAGAGATGTAAAGCACAAACTTTAAAt                                                                                                           >  2:1960106/1‑150 (MQ=255)
                                        tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATCAAAAAAAAAAATAAAAAAGAGATGTAAATCACTAATTTTAAAt                                                                                                           >  1:1507996/1‑150 (MQ=255)
                                         ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAAAATCGATGTAAATAACTAAGTTTAAAtt                                                                                                          >  1:2866256/1‑150 (MQ=255)
                                           cGGGGATGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACTGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                        <  2:3170184/150‑1 (MQ=255)
                                             gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                      <  2:2696534/150‑1 (MQ=255)
                                              ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                                                                                                     <  1:2952038/150‑1 (MQ=255)
                                               gAAGGATATAGATATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                    <  2:4046022/150‑1 (MQ=255)
                                                  ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAAAAGCGATGTAAATCAATAATTTTAAATTGCGCGCCaa                                                                                                 >  2:1427065/1‑150 (MQ=255)
                                                  ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAAAAAAAAAAATATCGATTTAAATCACTAAATTTAAATTGAGCGCCaa                                                                                                 >  2:39088/1‑150 (MQ=255)
                                                     tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                              <  2:3472532/150‑1 (MQ=255)
                                                      ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                             <  1:1339562/150‑1 (MQ=255)
                                                        agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATAAAAAAAAAAGCGCTGTAAATCACTAAGTTTAAAATTAGCGCCCCTCATgg                                                                                           >  1:4072290/1‑150 (MQ=255)
                                                         gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                          <  2:1627259/150‑1 (MQ=255)
                                                         gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                          <  2:3384363/150‑1 (MQ=255)
                                                           gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                        >  2:4351622/1‑150 (MQ=255)
                                                                  gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTATAAAATAATTAAAATAAATAAAGAAAAAAGCGATTTAAATCACTAAGTTTAAAATGCGCGCCAATCAATGCGCGCACAAg                                                                                 >  2:2104463/1‑150 (MQ=255)
                                                                  gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAATAAATCAAGACAAAAGCGATGTAAAGCACGAAGTTTAAATTGCGCGCCAAAAATGGCGCGCACAAg                                                                                 >  2:599615/1‑150 (MQ=255)
                                                                  gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATGTATAAAATAATTCAAAGAAAAAAACACAAAATCGCTGTGAAGCGCAAAGGTTAAATTGCGCGCCCAACATGGCGCGcacacg                                                                                 >  2:1654624/1‑148 (MQ=255)
                                                                  gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTCAAAAAAAAAAAGAAAAAAACGCTTTAAATCACAAAAATTTAATTTCGCGCCAAACAGGGCGCCCACaaa                                                                                 >  1:3586427/1‑149 (MQ=255)
                                                                   caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                <  2:3193511/150‑1 (MQ=255)
                                                                    aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                               >  2:3231894/1‑150 (MQ=255)
                                                                     agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                              >  2:2800341/1‑150 (MQ=255)
                                                                            atttGTAATTTATTTTTCGTTCGACCTTTGATTATACATAACAGTATTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                       <  2:2926224/150‑1 (MQ=255)
                                                                            atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                       <  2:652512/150‑1 (MQ=255)
                                                                              ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                     <  2:517919/150‑1 (MQ=255)
                                                                                    tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGACGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                               <  2:2123064/150‑1 (MQ=255)
                                                                                     gTATTTTTGGTTCGACATTTGCTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAACGCACTAAGTTTAATTTGCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                              <  1:3281795/150‑1 (MQ=255)
                                                                                      tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                             <  2:3571024/150‑1 (MQ=255)
                                                                                        tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                           <  2:3467213/150‑1 (MQ=255)
                                                                                        tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                           <  2:1043258/150‑1 (MQ=255)
                                                                                        tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGAAAATATCGATGTAAAGCACTAAGTTTAAAATGCGCGCCAATCATGGCGCGCACAATCTATAATACCAAACTAAATtct                                                           >  2:2755675/1‑150 (MQ=255)
                                                                                            tcgttcgACCTTTGCTTATACATAACAGTGGTTTTTTTAATTTATAAAATATTTCAAATAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCATTCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                       <  1:529166/150‑1 (MQ=255)
                                                                                               ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAATAGCGATGTAAATCACTAACTTTAAAATTCGCGCCAATAATGGCGCGCACAAGCTAAAATAACAACCTAATTTctcctccac                                                    >  2:3138725/1‑148 (MQ=255)
                                                                                               ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                    >  2:3269094/1‑150 (MQ=255)
                                                                                                tcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAACATGGCGCGCACAAGCTATAATACCAACCAAAATTCTCCACctct                                                   >  2:2415761/1‑150 (MQ=255)
                                                                                                  gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAAACAATACAATAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAAACATGTCGCGCACAAGCAATAAAAACAACCTAATTTatactcctaata                                                 >  2:2436920/1‑146 (MQ=255)
                                                                                                  gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATTTAAATCACTAAGTTTTAATTGCGCGCCAATAATGGCGCGCAAAACCTATAATACCAACCAAATTTATACAACTCTTa                                                 >  2:440091/1‑150 (MQ=255)
                                                                                                  gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCCCAACCTATAATACCCACCTAATTTgtcctccacata                                                 >  2:4118837/1‑145 (MQ=255)
                                                                                                  gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAAACAAGACAAAAGAGATGGAAAGCACAAAATTTAAATTGCGCGCCAAACCTGGCGCGCACAAGCTATAATACCAACCTAAGTTCTCCACCTCGTa                                                 >  2:2615505/1‑150 (MQ=255)
                                                                                                        tGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                           <  1:1978184/150‑1 (MQ=255)
                                                                                                        tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                           <  1:695996/150‑1 (MQ=255)
                                                                                                               aCATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCCAACAAGGCGCGCACAAGCAATAAAAACAAACAAATTTCTACTCCGCTTAGAGTGaagataac                                    >  2:4192/1‑143 (MQ=255)
                                                                                                                cATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGCTGTAAAGCGCTAAGTTTAAATTGCGCGCAAATCAAGGCGCGCACAAGCTATAATACCAACCAAAATTATCCTCCTCATAGAGTGACTATAtct                                   >  2:652717/1‑150 (MQ=255)
                                                                                                                  tAACAGTCGTTTTTTTAAATTATAAAAAAATTAAAAGAAATAAAGACAATAGCGATGTAAATCACAAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAACCAAATTTCTCCTCCTCTTAGAGTGACtataacaac                                 >  2:2188705/1‑145 (MQ=255)
                                                                                                                     ccgTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAAGACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                              <  1:614924/148‑1 (MQ=255)
                                                                                                                     cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                              <  1:3967304/150‑1 (MQ=255)
                                                                                                                     cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                              <  2:3540610/150‑1 (MQ=255)
                                                                                                                       ttCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                            <  2:2803804/149‑1 (MQ=255)
                                                                                                                       gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCTCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                            <  2:3351130/150‑1 (MQ=255)
                                                                                                                       gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                            <  2:2395946/150‑1 (MQ=255)
                                                                                                                       gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                            <  2:36037/150‑1 (MQ=255)
                                                                                                                          gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                         >  2:1110804/1‑150 (MQ=255)
                                                                                                                           tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                        >  1:2541571/1‑150 (MQ=255)
                                                                                                                            ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                       <  1:4185601/150‑1 (MQ=255)
                                                                                                                              ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                     <  1:3510772/150‑1 (MQ=255)
                                                                                                                              ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                     <  1:1784170/150‑1 (MQ=255)
                                                                                                                              ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                     <  2:408092/150‑1 (MQ=255)
                                                                                                                              ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                     >  2:4238513/1‑150 (MQ=255)
                                                                                                                              ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                     >  2:3093180/1‑150 (MQ=255)
                                                                                                                               tttattttatAATATATTTCAAAGAAATCAAGACAATAGCGATGTAAAGCAGTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:2827851/150‑1 (MQ=255)
                                                                                                                               tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:4063769/150‑1 (MQ=255)
                                                                                                                               tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:3061488/150‑1 (MQ=255)
                                                                                                                               tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    >  1:311539/1‑150 (MQ=255)
                                                                                                                               tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                    <  1:794501/150‑1 (MQ=255)
                                                                                                                                ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                   >  1:1746579/1‑150 (MQ=255)
                                                                                                                                  aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                 >  1:3684329/1‑150 (MQ=255)
                                                                                                                                    tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt               >  1:2322421/1‑150 (MQ=255)
                                                                                                                                      tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa             <  2:3516539/150‑1 (MQ=255)
                                                                                                                                       ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc            <  1:4273257/150‑1 (MQ=255)
                                                                                                                                        taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt           >  1:2145488/1‑150 (MQ=255)
                                                                                                                                         aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa          >  1:1510433/1‑150 (MQ=255)
                                                                                                                                              aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa     <  2:2542584/150‑1 (MQ=255)
                                                                                                                                                ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg   <  1:1542701/150‑1 (MQ=255)
                                                                                                                                                ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg   <  1:2441682/150‑1 (MQ=255)
                                                                                                                                                ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg   <  1:3007493/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGC  >  NC_000913/2470281‑2470575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: