Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,788,676 A→T 5.6% intergenic (+27/‑309) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,788,6760AT 5.6% 708.5 / 21.4 200intergenic (+27/‑309)ygaQ/csiDuncharacterized protein; putative enzyme/carbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (77/111);  new base T (8/3);  total (86/114)
Fisher's exact test for biased strand distribution p-value = 5.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 205 total aligned reads displayed.

AATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTTAA  >  NC_000913/2788529‑2788824
                                                                                                                                                   |                                                                                                                                                    
 aTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACgg                                                                                                                                                   <  2:2686071/150‑1 (MQ=255)
       cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCAATAAAACGGGGCAGa                                                                                                                                             <  1:1319632/150‑1 (MQ=255)
            tGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATCTCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGatataa                                                                                                                                        <  1:2992474/150‑4 (MQ=255)
             ggTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGatataac                                                                                                                                       <  1:341275/150‑5 (MQ=255)
              gTAAAGTATTTACGCTTATAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTGTAGGGAAAACAGGAGAACCGCCGAACTATATCTATTTATAATTTAATGTTATATATGCCCCGATAAAACGGGGCATATAATATg                                                                                                                                      >  2:1496934/1‑150 (MQ=255)
              gTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATg                                                                                                                                      >  2:2896000/1‑150 (MQ=255)
              gTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATg                                                                                                                                      >  2:1966111/1‑150 (MQ=255)
               tAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgt                                                                                                                                     >  2:282840/1‑150 (MQ=255)
                  aGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttta                                                                                                                                  <  2:949467/150‑1 (MQ=255)
                   gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                                 >  1:3591012/1‑150 (MQ=255)
                     aTTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagtt                                                                                                                               <  2:2302302/150‑1 (MQ=255)
                      tttACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagttt                                                                                                                              <  1:2238274/150‑1 (MQ=255)
                            cTTAGAGGCGGAACTCTAAATATCAATAGTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAAc                                                                                                                        <  2:752854/150‑1 (MQ=255)
                             ttAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACg                                                                                                                       <  2:3702597/150‑1 (MQ=255)
                               agagGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGt                                                                                                                     >  2:3364802/1‑150 (MQ=255)
                                 agGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCa                                                                                                                   >  2:3635896/1‑150 (MQ=255)
                                    cGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAttt                                                                                                                >  1:3257527/1‑150 (MQ=255)
                                      gAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTg                                                                                                              <  1:137366/150‑1 (MQ=255)
                                      gAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTg                                                                                                              <  2:4331724/150‑1 (MQ=255)
                                      gAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTAGCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTg                                                                                                              >  1:1058449/1‑150 (MQ=255)
                                       aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                             <  2:3736910/150‑1 (MQ=255)
                                        aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                            <  1:546903/150‑1 (MQ=255)
                                          tctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGt                                                                                                          <  2:3397660/150‑1 (MQ=255)
                                          tctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGt                                                                                                          <  2:809506/150‑1 (MQ=255)
                                           ctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTg                                                                                                         >  1:1895260/1‑150 (MQ=255)
                                             aaaTATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGaa                                                                                                       <  2:1008537/150‑1 (MQ=255)
                                               atatCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGc                                                                                                     >  1:1212339/1‑150 (MQ=255)
                                                   cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                                 <  1:142641/150‑1 (MQ=255)
                                                   cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                                 <  1:1157485/150‑1 (MQ=255)
                                                     atatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTa                                                                                               <  2:3864153/150‑1 (MQ=255)
                                                     atatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACGATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTa                                                                                               <  2:1615718/150‑1 (MQ=255)
                                                       atTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACt                                                                                             <  2:2207045/150‑1 (MQ=255)
                                                       atTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACt                                                                                             <  2:3657644/150‑1 (MQ=255)
                                                          ccTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGtt                                                                                          <  2:1210566/150‑1 (MQ=255)
                                                           cTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGAAGTGAAGCCATTTACTGttt                                                                                         >  1:3814622/1‑150 (MQ=255)
                                                            tGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAAGTTTCTCTATTTATAATTTATTGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGtttt                                                                                        >  1:2614845/1‑150 (MQ=255)
                                                            tGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGtttt                                                                                        >  1:945331/1‑150 (MQ=255)
                                                               gCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTa                                                                                     <  1:378943/150‑1 (MQ=255)
                                                               gCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTa                                                                                     <  2:453509/150‑1 (MQ=255)
                                                                cGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTAt                                                                                    <  1:1523988/150‑1 (MQ=255)
                                                                cGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTAt                                                                                    <  1:2376792/150‑1 (MQ=255)
                                                                   aGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGa                                                                                 <  1:3636897/150‑1 (MQ=255)
                                                                    gcgcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGAc                                                                                <  2:2566580/150‑1 (MQ=255)
                                                                       ctctTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAg                                                                             >  1:743741/1‑150 (MQ=255)
                                                                          ttcttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATa                                                                          >  1:1248357/1‑150 (MQ=255)
                                                                            cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                        <  2:3150488/150‑1 (MQ=255)
                                                                            ctattACGGAAAACACGAGAACCGCCCAACAATCACAATTTATAATTTAATGTTATATCTGCCCCGAAAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAACCCAATTACTGATTTTTATCGACCAGATAAt                                                                        <  2:458692/147‑1 (MQ=255)
                                                                             ttttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATc                                                                       >  1:2666524/1‑150 (MQ=255)
                                                                             ttttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATc                                                                       >  2:3727962/1‑150 (MQ=255)
                                                                             ttttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATc                                                                       <  1:3574253/150‑1 (MQ=255)
                                                                              tttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCt                                                                      >  2:2167504/1‑150 (MQ=255)
                                                                              tttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGAGAATCt                                                                      >  2:401573/1‑150 (MQ=255)
                                                                               ttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTg                                                                     <  1:3077714/150‑1 (MQ=255)
                                                                                tAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGt                                                                    <  1:2751339/150‑1 (MQ=255)
                                                                                tAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGt                                                                    <  2:3931971/150‑1 (MQ=255)
                                                                                  gggAAAACAGGAGAACCGCCGAACTCTCTCTATTTATCATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGGTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCCTTTCCTGTTTTTTATCGACCAGAGAAGCTGTtc                                                                  >  1:2791334/1‑150 (MQ=255)
                                                                                     aaaaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTctct                                                               <  2:2618516/150‑1 (MQ=255)
                                                                                      aaaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTCTCGACCAGATAATCTGTTCTCTa                                                              <  2:1524817/150‑1 (MQ=255)
                                                                                      aaaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTa                                                              <  2:1127029/150‑1 (MQ=255)
                                                                                       aaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTaa                                                             <  1:206463/150‑1 (MQ=255)
                                                                                        aCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAt                                                            <  2:2516390/150‑1 (MQ=255)
                                                                                          aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCTACCAGATAATCTGTTCTCTAATGt                                                          >  2:3056373/1‑150 (MQ=255)
                                                                                          aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATCATCTGTTCTCTAATGt                                                          >  2:740047/1‑150 (MQ=255)
                                                                                          aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGt                                                          >  2:499042/1‑150 (MQ=255)
                                                                                              gaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAAc                                                      <  1:1791409/150‑1 (MQ=255)
                                                                                               aaCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACt                                                     >  1:3905040/1‑150 (MQ=255)
                                                                                                 ccgccgAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTcc                                                   <  1:302340/150‑1 (MQ=255)
                                                                                                 ccgccgAAATATCTATATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCATATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTcc                                                   <  1:2165865/150‑1 (MQ=255)
                                                                                                  cgccgAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTccc                                                  <  1:2496492/150‑1 (MQ=255)
                                                                                                        aCTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAAc                                            <  2:4185515/150‑1 (MQ=255)
                                                                                                              tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTg                                      >  2:2434362/1‑150 (MQ=255)
                                                                                                              tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTg                                      >  2:2932011/1‑150 (MQ=255)
                                                                                                               ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTATCCTGTTGc                                     >  2:4253245/1‑150 (MQ=255)
                                                                                                                   ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                                 >  2:2110759/1‑150 (MQ=255)
                                                                                                                         tttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAtt                           <  1:986997/150‑1 (MQ=255)
                                                                                                                           tAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCa                         >  1:2138286/1‑150 (MQ=255)
                                                                                                                            aacatattatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAt                        >  1:3564314/8‑150 (MQ=255)
                                                                                                                            aacatattatCTGCCCCGATTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCACCCTAACCTGTTGCTTTAGTTATTAAt                        >  1:3322252/8‑150 (MQ=255)
                                                                                                                            aaTGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAt                        <  2:1682177/150‑1 (MQ=255)
                                                                                                                            aaTGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAt                        <  2:3180144/150‑1 (MQ=255)
                                                                                                                            aaTGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAt                        <  2:176777/150‑1 (MQ=255)
                                                                                                                               gTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTc                     >  1:1873079/1‑150 (MQ=255)
                                                                                                                                ttATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTcc                    >  1:650187/1‑150 (MQ=255)
                                                                                                                                  ttatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTg                  >  2:3005117/2‑150 (MQ=255)
                                                                                                                                   tatCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGt                 >  1:4302678/1‑150 (MQ=255)
                                                                                                                                   tatCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGATCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGt                 >  1:4302616/1‑150 (MQ=255)
                                                                                                                                     tCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct               >  1:753410/1‑150 (MQ=255)
                                                                                                                                     tCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct               >  2:275434/1‑150 (MQ=255)
                                                                                                                                      cTGCCCCGTTTTATCGGGGCCGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATCATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtctc              >  2:1813856/1‑150 (MQ=255)
                                                                                                                                       tGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCa             >  2:451349/1‑150 (MQ=255)
                                                                                                                                       tGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTACTGTCTCa             >  1:1629948/1‑150 (MQ=255)
                                                                                                                                        gCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCtttattta            <  2:2927784/150‑8 (MQ=255)
                                                                                                                                        gCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCtttattta            <  2:1639732/150‑8 (MQ=255)
                                                                                                                                        gCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCtttattta            <  2:2927848/150‑8 (MQ=255)
                                                                                                                                            cGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTg        >  1:1728597/1‑150 (MQ=255)
                                                                                                                                             gATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGc       <  1:293019/150‑1 (MQ=255)
                                                                                                                                             gATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGc       <  2:136489/150‑1 (MQ=255)
                                                                                                                                              aTAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGcc      <  2:2144392/150‑1 (MQ=255)
                                                                                                                                                  aaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTTaa  <  2:633420/150‑1 (MQ=255)
                                                                                                                                                  aaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTTaa  <  1:916414/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTTAA  >  NC_000913/2788529‑2788824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: