BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	5.1%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	5.1%	771.5 / 24.8	241	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (101/126); new base G (8/5); total (110/131)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 244 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

    gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                                   <  2:1310472/150‑1 (MQ=255)

        cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGAT                                                                                                                                               <  2:841939‑M1/150‑1 (MQ=255)

         ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAGGAGATG                                                                                                                                              >  1:861144‑M1/1‑150 (MQ=255)

         ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATG                                                                                                                                              >  1:2983237‑M1/1‑150 (MQ=255)

         ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATG                                                                                                                                              <  1:1444972‑M1/150‑1 (MQ=255)

         ggAGAATATGGTGCATCCGGGAGGATTCGAACATCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCAGCTCAATTAGGAGATG                                                                                                                                              >  1:4338016‑M1/1‑150 (MQ=255)

            gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                           <  1:1611836‑M1/150‑1 (MQ=255)

            gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                           <  1:386053‑M1/150‑1 (MQ=255)

               tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTACGAGATGGTGCAT                                                                                                                                        >  1:2237243‑M1/1‑150 (MQ=255)

               tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                        >  1:3948894‑M1/1‑150 (MQ=255)

               tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAT                                                                                                                                        >  2:1982675‑M1/1‑150 (MQ=255)

                        tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCAGCGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGA                                                                                                                               <  2:1488974‑M1/150‑1 (MQ=255)

                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                             <  2:3256855‑M1/150‑1 (MQ=255)

                           gggAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTa                                                                                                                            <  2:3928673/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          <  2:2567542/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          >  2:2828462/1‑150 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          <  2:2980953/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          <  2:2970670/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          <  1:1925971/150‑1 (MQ=255)

                              atgaTTCGAACCTCCGACCGCTAGGTTCGTAGCCGTGTTCTCTTTGCATCTGAGCTACTGATTCATCGTGATCACTTATTCTACTGCTGATATTGAGACCGCTACTAAAGCCATATCAATTATGAGATGGTGCATCCGGGAGGATTACTc                                                                                                                         <  1:607413/148‑1 (MQ=14)

                                     gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGc                                                                                                                  >  1:757165/1‑150 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 <  2:3653335/150‑1 (MQ=255)

                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                <  1:1628493/150‑1 (MQ=255)

                                         cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCt                                                                                                              <  2:4064959/150‑1 (MQ=255)

                                         cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCAAATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCAGTGCGGCt                                                                                                              >  1:2321938/1‑150 (MQ=255)

                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCTTCTGAGCTACGTATTCATCGTGACAACATATTCTACTGCTGATATTGATACCTCTACTAACGCCATATCAATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  1:1701801/150‑1 (MQ=17)

                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  1:659470/150‑1 (MQ=255)

                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  2:284659/150‑1 (MQ=255)

                                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg                                                                                                     <  1:2539928/150‑1 (MQ=255)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                               <  1:1866581/150‑1 (MQ=255)

                                                            aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                                                           <  2:4227619/150‑1 (MQ=255)

                                                            aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                                                           >  2:3424607/1‑150 (MQ=255)

                                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGCTTATTCTACTGCGGAAATTGATACCGCTACGACACCCCTATCCAAAAAGAGGTCGTCCATCCGGGAGGCTCACTCGCCGGCCGGCTCGCCCTTCGGGCCGTTGCtca                                                                                         >  2:3382836/1‑148 (MQ=14)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:3363555/150‑1 (MQ=255)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:4278163/150‑1 (MQ=255)

                                                                gAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagta                                                                                            <  2:1644247‑M1/145‑34 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                                                                 >  1:2089992/1‑150 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                                                                 >  2:215300/1‑150 (MQ=255)

                                                                         aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                                                              <  1:1142025/150‑1 (MQ=255)

                                                                         aTCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                                                              >  2:1186186/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTAAGGATTCATCGGGAATACTTATTCTACTGCTTATATTGATACAGATACTACAGCCATATATATTAAGAGATGGTGCATCCGGGAGTATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                                                             <  2:2719061/150‑1 (MQ=17)

                                                                          tCCAGATGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                                                             >  2:3945169/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTAATTCAACTCCTGATATTGATAACCCAACTAAACCCATATCAATTAAGAGAAGCTGCATCCCGGAGGATTACTCCCCATGCGGCTACCCGTGGGGGACGTAGCTAAAGCCCCGTTataatc                                                                          >  2:2413155/1‑150 (MQ=12)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                         >  1:291829/1‑150 (MQ=255)

                                                                                   gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGTTATTGAGACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  2:3310503/150‑1 (MQ=255)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                   >  1:2707996/1‑150 (MQ=255)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                   >  1:98560/1‑150 (MQ=255)

                                                                                        ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                                                               <  2:757165/150‑1 (MQ=255)

                                                                                            gCATCGGGAAAACTTATTCTACTGCTGATATTGCTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTCGTGCCTGCGGt                                                           <  2:4287869/150‑1 (MQ=255)

                                                                                            gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                           <  2:3804383/150‑1 (MQ=255)

                                                                                              aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTACGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTa                                                         >  1:2858618/1‑150 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        >  1:4006258/1‑150 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        >  1:4244027/1‑150 (MQ=255)

                                                                                               ccGGGAAAACTTATTCTACTGCTGAGATTGATACCGCTACTAAAGCCATATCAATTAAGAGCTGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        <  2:3770788/149‑1 (MQ=255)

                                                                                                   gAAAACTTCTTCTACTGCTGATATTGATACCGCTACTACCGCCATCTCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                    <  2:2237143/150‑1 (MQ=255)

                                                                                                   gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                    <  1:1982675/150‑1 (MQ=255)

                                                                                                    aaatCTTATTCTACTTATTTAATTGATACAGCTACTACAGCGATATTTATTTTTTGATTGTGCCTACGGGAGTTTTTCTCTCCTTGAGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:2265973/150‑1 (MQ=9)

                                                                                                    aaatCTTATTCGACTTCTGATATTGATAGCGCTAATATAGCCATATCAATTTAGATATGGTGCATCCGGGAGGTTTAGTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:3191325/150‑1 (MQ=17)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:2266349/150‑1 (MQ=255)

                                                                                                     aaccatATTCGACTGCTGATACTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAGTACTCGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTACAGCACCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                  <  2:2257806/145‑1 (MQ=17)

                                                                                                       aCTTATTCTACTGCTGAGATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa                                                <  1:4090569/150‑1 (MQ=255)

                                                                                                        tttaTTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtga                                                       <  2:4250929‑M1/141‑71 (MQ=255)

                                                                                                        cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                               <  1:3424607/150‑1 (MQ=255)

                                                                                                        cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                               >  2:3976432/1‑150 (MQ=255)

                                                                                                        cTTATTCCACTGCTGATATAGATCCCGCTACTAATCCCATATATTTTACTAGATTGTGCTTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                               <  2:478866/150‑1 (MQ=18)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                              >  1:1169371/1‑150 (MQ=255)

                                                                                                              cTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  1:4190813/150‑1 (MQ=255)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                        >  1:3669880/1‑150 (MQ=255)

                                                                                                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                       >  2:1204586/1‑150 (MQ=255)

                                                                                                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGa                                       >  2:3860742/1‑150 (MQ=255)

                                                                                                                  cgcttaTATTTATACAGCTACTAATCCGATATAAAGTACTAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaacccc                                     <  1:1242552‑M1/149‑89 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                                    <  1:3964647/150‑1 (MQ=255)

                                                                                                                         atTTATACCGCTACTAAACCGATATCAAGTAAGAGACGGAGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgca                              <  2:1906675‑M1/150‑96 (MQ=255)

                                                                                                                         atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGAGCCTCGCCCTTAGGGCCGTTGCTAAAGCAACGTTATCATACCTAGTGCATGCGGTTAATTCCGCGACCTCTTCGATACCTTCAt                              >  2:252401/1‑150 (MQ=14)

                                                                                                                           tGATACCGCTACTACAGCCTTTTCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTATAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                            <  1:1190726/150‑1 (MQ=255)

                                                                                                                             aTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCt                          <  1:2126415/150‑1 (MQ=255)

                                                                                                                             aTACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCt                          <  2:1350134/150‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       >  2:4002540/1‑150 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                      >  2:3238408/1‑150 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCCTTGCGGCTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                      >  1:3781550/1‑150 (MQ=255)

                                                                                                                                 cGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttga                      <  2:172711‑M1/150‑104 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     <  1:485550/150‑1 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     >  1:1771172/1‑150 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATACGGGAGGATTACTCTCCTTTCTGCTCGCCCTTCGGGCCGTTGCGATAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     <  1:4220962/150‑1 (MQ=17)

                                                                                                                                  gCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggacgagaggcggggattcgaaccaatgatgcagcttttgac                     >  2:596005‑M1/1‑46 (MQ=255)

                                                                                                                                    tactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccg                   >  2:913192‑M1/1‑44 (MQ=255)

                                                                                                                                    aactaaAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctacgaccgcacggatcgtagcagagtactctatccagctgagctacggatgcaaatgccgggcacccggcgattcgaaaaccggatgcagattttgaccg                   >  2:1257056‑M1/2‑44 (MQ=255)

                                                                                                                                     actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                  <  1:3239395/150‑1 (MQ=255)

                                                                                                                                     actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                  <  1:2467726/150‑1 (MQ=255)

                                                                                                                                        aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                      <  2:141504/63‑1 (MQ=40)

                                                                                                                                        aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                      >  1:141504/1‑63 (MQ=40)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTATTACCGc                                                  >  1:3594113/1‑114 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              >  1:131353/1‑150 (MQ=255)

                                                                                                                                               tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat        >  2:2638975/1‑150 (MQ=255)

                                                                                                                                               tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat        >  1:422086/1‑150 (MQ=255)

                                                                                                                                               tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat        >  2:699813/1‑150 (MQ=255)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                <  2:3681701/45‑1 (MQ=38)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg    >  2:4148917/1‑150 (MQ=255)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGATGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCACTTCGATAACTTCATTGCTGAAGATTTCGGAGAATATGGTg    >  1:3794814/1‑150 (MQ=255)

                                                                                                                                                    aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTGCAATGCTGAAGATTTCGGAGAATATGGTGc   >  1:4140864/1‑150 (MQ=255)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`