BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	7.9%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	7.9%	610.3 / 30.6	184	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (97/72); new base A (7/8); total (104/80)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817900‑2818198

actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                       <  2:2726961/150‑1 (MQ=255)

 ctaAAGCAATATCAATTATGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                      <  2:1201640/150‑1 (MQ=255)

    aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   >  1:131353/1‑150 (MQ=255)

         atatCAATTAAGAGATGGTGCAGCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAGGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAata                                                                                                                                              <  1:4227313/150‑1 (MQ=255)

          tatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                             >  1:422088/1‑150 (MQ=255)

               aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTGCAATGCTGAAGATTTCGGAGAATATGGTGc                                                                                                                                        >  1:4140864/1‑150 (MQ=255)

                ttAAGAGATGGTGCATCCGGGAGGATTACTCCCCGGGCGCCGCCCCCTACGGGCCGATGCGAAAGCACCGATATCATCCCTAGGCCCAGCGCAAAATACCGCTACCACTTCGATACCTACATTGCTGAAGAGTTCGGAGAAAATGGTGCa                                                                                                                                       >  2:3668262/1‑150 (MQ=12)

                       aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                                                                                                <  2:291829/150‑1 (MQ=255)

                        tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggag                                                                                                                               >  1:3500285/1‑150 (MQ=255)

                          gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                             >  1:2681196/1‑150 (MQ=255)

                            gcccccGGGTGGAGTTGTCGCAGTGAGGCTCGCCCTTCGGGCCGTTGCTACAGCAACGTTATCATCCCTTGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                           <  1:2590182/146‑1 (MQ=17)

                            gCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGTTATCTTCCCTAGTGCCTGCGGTTAATTGAGCTACCACGTCGATACCTCCATTGCTGAAGACTTCGGAGAACATGGTGCATCCGGGAGGAtt                                                                                                                           <  2:1239499/150‑1 (MQ=17)

                             cacccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:2983237/147‑1 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:2370620/150‑1 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTCGCGGCTCGCCCGTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTACTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:17605/150‑1 (MQ=255)

                             cATCCGGGAGGATTACTCGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:861144/150‑1 (MQ=255)

                             cATCCGCGAGGACTACTCGCCACGCGGCTCGCCCCCCGGGCCGTTGCTAAAGCTTCGTTCTCCTCCCCAGTGACTGAGGCTACTTCCGCTACCAATTCGATACCCTCAGCTCTTCAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                          <  2:4338016/150‑1 (MQ=9)

                                  gggAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                                     >  1:1075189/1‑150 (MQ=255)

                                       gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                                <  1:4297643/150‑1 (MQ=255)

                                       gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                                <  1:1186259/150‑1 (MQ=255)

                                       gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                                <  1:3707653/150‑1 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          >  1:511306/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          >  2:3465122/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          >  2:4046544/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          >  1:2270256/1‑150 (MQ=255)

                                              cGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                                                         <  2:2243020/150‑1 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTTCTAAAGCAACGTTATCATCCCTAGTGACTGCTGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                        <  2:495829/150‑1 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTCAGCAACGTTATCCTCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                        <  2:679304/150‑1 (MQ=255)

                                                 cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGTTTAATTCCGCTTCCTCTTCGATACCTTCATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                      <  1:3945169/150‑1 (MQ=255)

                                                 cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                      <  1:3945141/150‑1 (MQ=255)

                                                  ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     <  1:3238408/150‑1 (MQ=255)

                                                  ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCAAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     >  1:2803683/1‑150 (MQ=255)

                                                  ttGCGGCTCGCCCTTAGGGCCGTTGCTAAAGCAACGTTATCATCCCAAGTGCCAGCGGTTAATTCCGCAACCAATTAGATACCTTAATTGCTGAAGATTTCGGAGAATAAGGTGCATCCGGGAGGATTCGTACCTCCGACCGCTCGGGTc                                                                                                     >  1:829504/1‑150 (MQ=17)

                                                      ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                                                 >  2:3968377/1‑150 (MQ=255)

                                                        cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                               >  1:4298632/1‑150 (MQ=255)

                                                           gCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTACGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAAACTCCGACCGCTCGCTTCGTAGCCGAg                                                                                            >  1:452434/1‑150 (MQ=255)

                                                           gCCCTTCGGGCCGTTGCAAAAGCAACGTTATCAACCATAGTGCCTGCGGTTAATTCCGCTACCAATTCGAAACCTTCATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCACGGTTCGTAGCCGAg                                                                                            >  1:544774/1‑150 (MQ=17)

                                                            cccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGt                                                                                           >  2:3995813/1‑150 (MQ=255)

                                                               ttCGGGCCGTTGCTAAAGCAACGTTATAAACAAGAGTGCATGCGGTTAATTCCGCTAACTCTTCGAAACCTTCATTGAAGAAGATTTCGCAGAATATGTTGCATCCGGGAGGATTCGAACCTCCGACCGCGCGGTGCGTAGCCGGGtcgg                                                                                        >  2:2381055/1‑147 (MQ=17)

                                                                tCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                                                                       <  2:4198432/150‑1 (MQ=255)

                                                                tCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                                                                       <  2:4244027/150‑1 (MQ=255)

                                                                   taacGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                                                    >  1:3612903/4‑150 (MQ=255)

                                                                      cGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                                                                 <  2:422086/150‑1 (MQ=255)

                                                                          gCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                             >  1:567706/1‑150 (MQ=255)

                                                                          gCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                             >  1:2322300/1‑150 (MQ=255)

                                                                           cTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                            >  1:2091057/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  1:3149384/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  1:2013368/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                           >  1:3338513/1‑150 (MQ=255)

                                                                               aGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                        >  2:286714/1‑150 (MQ=255)

                                                                                  aaCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTCCgg                                                                     >  1:925962/1‑150 (MQ=255)

                                                                                  aaCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                     >  1:1982278/1‑150 (MQ=255)

                                                                                           atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                            >  1:2867123/1‑150 (MQ=255)

                                                                                           atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                            <  2:1075189/150‑1 (MQ=255)

                                                                                              ccgagggCCTGCGGTGACTTCCGCTACCTCTCCGATCCCGTCAGTGCTGACGTCTTCGGAGCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa                                                         <  2:4140864/144‑1 (MQ=11)

                                                                                                tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                       >  1:1223791/1‑150 (MQ=255)

                                                                                                tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                       >  1:3397455/1‑150 (MQ=255)

                                                                                                tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                       >  2:1775348/1‑150 (MQ=255)

                                                                                                   tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattta                                                    >  2:2310707/1‑150 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttattt                                                 >  1:1628392/1‑150 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttattt                                                 <  1:699813/150‑1 (MQ=255)

                                                                                                       tGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATGCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTCTCCAGCTGAGCTACGGATGCATCGGGAAAGTTAtttt                                                >  2:3365737/1‑150 (MQ=255)

                                                                                                           gTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActg                                            >  1:972734/1‑150 (MQ=255)

                                                                                                            ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                           >  1:2367047/1‑150 (MQ=255)

                                                                                                                 tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAACTTTATTTTACTGCTGata                                      >  1:3890199/1‑150 (MQ=255)

                                                                                                                  ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGAATCGGGAAATTTATTTTACTGCTGatat                                     >  2:1721892/1‑150 (MQ=255)

                                                                                                                   cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    <  1:3270242/150‑1 (MQ=255)

                                                                                                                   cGCTACCTCGTCGATACCTTCATTGCTGAAGATTTCGGAGAATCTGGTGCATCCGGGAGGTTTCGACCCTCCGCCCGCTCGGTTCGGAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    <  1:3382836/150‑1 (MQ=14)

                                                                                                                    gCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                             <  2:4255828/140‑1 (MQ=9)

                                                                                                                    gCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                             >  1:4255828/1‑140 (MQ=9)

                                                                                                                    gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGTGAGGATTCGATCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTg                                   <  2:3600849/150‑1 (MQ=255)

                                                                                                                     cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                  <  1:1898790/150‑1 (MQ=9)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                  <  1:4060678/150‑1 (MQ=25)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                  >  1:3488436/1‑150 (MQ=255)

                                                                                                                      tatctctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                                 >  1:3162986/4‑150 (MQ=9)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCGGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                             >  2:1915783/1‑150 (MQ=16)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCGGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                             >  2:3812159/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCGCGCTTCGTAGCCGAGTACTCTATCCAGATGAGCTACGGAGGCATCGGGAAAATTAGTTTACGGAGGATATTGagaacg                             >  2:2639090/1‑145 (MQ=11)

                                                                                                                           ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGc                            <  1:215300/150‑1 (MQ=255)

                                                                                                                             tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCACTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          <  2:2171798/150‑1 (MQ=255)

                                                                                                                               gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctact                        >  1:1554143/1‑150 (MQ=255)

                                                                                                                                aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGATATTTTTAGCGctcaac                       >  2:1245885/1‑146 (MQ=11)

                                                                                                                                 tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaa                      >  2:2529243/1‑150 (MQ=255)

                                                                                                                                  aCCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  2:3162986/150‑1 (MQ=18)

                                                                                                                                  aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                            <  2:554573/95‑1 (MQ=37)

                                                                                                                                   ccTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg                    <  2:3345990/150‑1 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                  <  1:3671255/150‑1 (MQ=255)

                                                                                                                                       aaTTGATGAAGTTTACGTTGAATATGGTGAATCCGGGAGGATTCGAACCTCCTACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGATGAGCTACGGATGAATCGGTAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat                <  2:1835459/149‑1 (MQ=11)

                                                                                                                                        aTTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata               <  1:3747479/150‑1 (MQ=255)

                                                                                                                                        aTTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata               <  1:1182549/150‑1 (MQ=38)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCCACCGCTCGGTTCGTAGCCCAGTACTCTATACAGCTCAGCTACGGATGCATCGGGAAAATTAATTTACTGATGATATAGATACCGCTACTAAAGCCata               >  2:2571306/1‑150 (MQ=11)

                                                                                                                                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa           >  1:1710708/1‑150 (MQ=255)

                                                                                                                                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa           <  2:774372/150‑1 (MQ=255)

                                                                                                                                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACACAATCAAGCATACATACGCATGCATCGGGAACTTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCag           >  1:2949225/1‑149 (MQ=14)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACAGCTACTAAAGACATATCAAg          >  1:3231000/1‑150 (MQ=255)

                                                                                                                                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGGaaa                                                        >  1:1922808/1‑103 (MQ=25)

                                                                                                                                                aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCGGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTaa       <  1:3812159/150‑1 (MQ=255)

                                                                                                                                                 gttttCGGAGAATTTGGTGGATGTGTGAGGTTTGGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTAATATTGATACCGCTACTAAAGCCATATCAAGTAag      <  1:865808/148‑1 (MQ=14)

                                                                                                                                                 gATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAag      <  1:1435547/150‑1 (MQ=255)

                                                                                                                                                     tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt  >  1:128390/1‑150 (MQ=255)

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817900‑2818198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`