Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,828,537 A→T 6.1% intergenic (+21/‑84) srlD → / → srlM sorbitol‑6‑phosphate dehydrogenase/sorbitol=responsive srl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,828,5370AT 6.1% 786.7 / 24.9 220intergenic (+21/‑84)srlD/srlMsorbitol‑6‑phosphate dehydrogenase/sorbitol=responsive srl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (111/95);  new base T (2/12);  total (113/107)
Fisher's exact test for biased strand distribution p-value = 4.75e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

AAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTG  >  NC_000913/2828388‑2828685
                                                                                                                                                     |                                                                                                                                                    
aaaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTaa                                                                                                                                                      <  1:300196/150‑1 (MQ=255)
aaaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTaa                                                                                                                                                      >  2:2260657/1‑150 (MQ=255)
 aaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAg                                                                                                                                                     <  2:3652216/150‑1 (MQ=255)
 aaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAg                                                                                                                                                     <  1:1862348/150‑1 (MQ=255)
    tACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                  <  2:1727409/150‑1 (MQ=255)
    tACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAt                                                                                                                                                  <  1:3982981/150‑1 (MQ=255)
      ccGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGAtct                                                                                                                                                <  2:1056913/150‑1 (MQ=255)
       cGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCTTAATGGAtctc                                                                                                                                               <  2:903217/150‑1 (MQ=255)
        gCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTcc                                                                                                                                              >  1:4069947/1‑150 (MQ=255)
         cTCAAACGCGGCTGTGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                             <  1:3813497/150‑1 (MQ=255)
         cTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCg                                                                                                                                             <  1:2190111/150‑1 (MQ=255)
          tCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCTGGTGATGTTCTGATCAACAGCGGAGATCCATGAAGGATCTCCGt                                                                                                                                            >  1:411436/1‑150 (MQ=255)
          tCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCTTAATGGATCTCCgc                                                                                                                                            <  2:2425610/150‑2 (MQ=255)
          tCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGt                                                                                                                                            >  1:2199899/1‑150 (MQ=255)
            ccaCGCGGCTGCGATTATCAAGCTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCTTAATGGATCTCCgctg                                                                                                                                          <  2:3533419/148‑4 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCTTAATGGATCTCCgctg                                                                                                                                          <  2:591061/150‑4 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                          <  2:476568/150‑1 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                          <  1:841373/150‑1 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                          <  2:1910165/150‑1 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                          <  1:950011/150‑1 (MQ=255)
            aaaCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTga                                                                                                                                          >  1:2725343/1‑150 (MQ=255)
              aCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTgaga                                                                                                                                        <  1:1901575/150‑1 (MQ=255)
                  ggCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACtat                                                                                                                                    <  2:3374745/150‑1 (MQ=255)
                   gCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACtata                                                                                                                                   <  1:2007720/150‑1 (MQ=255)
                             tCAAGATGTGCTGAATATGCTGCTGTTCTAAGCCAGTCCTAAGGCGTCGTACTGAACAGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCAGTGAGACTATAGAATGCCTGa                                                                                                                         >  2:3712534/1‑150 (MQ=255)
                               aaGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATg                                                                                                                       <  1:3577684/150‑1 (MQ=255)
                                  aTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGACTGCCTGATgcgc                                                                                                                    <  2:33159/150‑1 (MQ=255)
                                  aTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcgc                                                                                                                    <  1:14054/150‑1 (MQ=255)
                                  aTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcgc                                                                                                                    <  1:3139587/150‑1 (MQ=255)
                                  aTGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATgcgc                                                                                                                    <  1:367699/150‑1 (MQ=255)
                                       cTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATAAAGGATCTCCGTGAGACTAAAGAATGCCTGATGCGCTACGc                                                                                                               >  1:3920481/1‑150 (MQ=255)
                                               gctgctGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCTATGTCACCGGCGGTCAGTTGATGTTCTGATCAACTGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATTCGCTACGCTCATCAgg                                                                                                       <  2:4260600/150‑1 (MQ=255)
                                                ctgctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGc                                                                                                      <  1:3753524/150‑1 (MQ=255)
                                                ctgctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGc                                                                                                      <  1:3272864/150‑1 (MQ=255)
                                                ctgctgTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGc                                                                                                      <  1:3423182/150‑1 (MQ=255)
                                                      ttCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAg                                                                                                >  2:1683831/1‑150 (MQ=255)
                                                      ttCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAg                                                                                                >  2:2333111/1‑150 (MQ=255)
                                                        cTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGa                                                                                              <  1:1217992/150‑1 (MQ=255)
                                                         tACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACAGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGAc                                                                                             >  1:1754962/1‑150 (MQ=255)
                                                                   cTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCCTTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCAGCAGGCATACAGGACTTCCGCCACt                                                                                   >  1:4219320/1‑150 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  >  2:2937337/1‑150 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  >  2:1201295/1‑150 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  >  2:811844/1‑150 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  <  1:76009/150‑1 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  >  2:3840571/1‑150 (MQ=255)
                                                                    tAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTa                                                                                  >  2:2937254/1‑150 (MQ=255)
                                                                     aaGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTAc                                                                                 <  1:1173189/150‑1 (MQ=255)
                                                                       ggCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACAt                                                                               <  1:2041725/150‑1 (MQ=255)
                                                                           tcgtACTGCACCGGACAGTCGATCAATGGCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAg                                                                           <  2:1222625/150‑1 (MQ=255)
                                                                                cTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGaaaa                                                                      <  2:434963/150‑1 (MQ=255)
                                                                                 tGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAg                                                                     >  2:518869/1‑150 (MQ=255)
                                                                                  gCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGt                                                                    >  1:4168872/1‑150 (MQ=255)
                                                                                  gCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGt                                                                    >  1:161133/1‑150 (MQ=255)
                                                                                   cACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGtt                                                                   >  2:3909673/1‑150 (MQ=255)
                                                                                   cACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGtt                                                                   >  2:3265698/1‑150 (MQ=255)
                                                                                    aCCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTa                                                                  <  1:1591228/150‑1 (MQ=255)
                                                                                    aCCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTa                                                                  <  2:3086598/150‑1 (MQ=255)
                                                                                       ggACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATgg                                                               <  2:981664/150‑1 (MQ=255)
                                                                                            gTCGATCAATGTCACCGGCGGTCAGGTGAGGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACGAGAGAATGCCTGATGCGCTACGCTCATCAGGCATACCGGACTTCCGCCACTCCAGTAAGGAAAAGTTCTGGTATcc                                                          >  2:2772096/1‑150 (MQ=255)
                                                                                             tCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCg                                                         >  1:2811571/1‑150 (MQ=255)
                                                                                             tCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCg                                                         >  2:2703560/1‑150 (MQ=255)
                                                                                               gATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCa                                                       >  2:2615995/1‑150 (MQ=255)
                                                                                               gATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTGATGGTATCCGCa                                                       >  1:3348332/1‑150 (MQ=255)
                                                                                                aTCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCAc                                                      >  1:2415893/1‑150 (MQ=255)
                                                                                                aTCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCAc                                                      >  1:4304206/1‑150 (MQ=255)
                                                                                                   aaTGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtca                                                   >  2:1127493/1‑150 (MQ=255)
                                                                                                     tGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatc                                                 >  1:2362263/1‑150 (MQ=255)
                                                                                                      gTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcatca                                                <  2:708355/150‑1 (MQ=255)
                                                                                                       tCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCAc                                               >  1:289252/1‑150 (MQ=255)
                                                                                                       tCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCAc                                               >  1:1023570/1‑150 (MQ=255)
                                                                                                           cggcggTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTc                                           >  2:1383010/1‑150 (MQ=255)
                                                                                                                gTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGt                                      >  1:1460578/1‑150 (MQ=255)
                                                                                                                  cAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGGCGCCGTTa                                    >  2:1318247/1‑150 (MQ=255)
                                                                                                                   aGGTGATGTTCTGTTCAACAGCGGAGATCCATTCAGGATCTCCGTGAGACTTTAGACTGCCTGATGCGCTTCGCTCATCAGGCATACAGGACTTCCGGCTCTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAt                                   <  1:2903121/150‑1 (MQ=255)
                                                                                                                   aGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTAt                                   <  1:1578597/150‑1 (MQ=255)
                                                                                                                     gTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCg                                 >  1:2236923/1‑150 (MQ=255)
                                                                                                                     gTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCAGCACCGTCGCCGTTATCg                                 >  2:1517497/1‑150 (MQ=255)
                                                                                                                       gATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGcc                               <  2:771692/150‑1 (MQ=255)
                                                                                                                       gATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGcc                               <  2:771523/150‑1 (MQ=255)
                                                                                                                       gATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGcc                               <  1:1683831/150‑1 (MQ=255)
                                                                                                                        aTGTTCTGCTCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCt                              <  2:2595188/150‑1 (MQ=255)
                                                                                                                        aTGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCt                              <  1:2306169/150‑1 (MQ=255)
                                                                                                                         tGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTg                             <  2:2630039/150‑1 (MQ=255)
                                                                                                                         tGTTCTGATCAACAGCGGAGATCCATTAAGGATATCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTg                             <  2:2306013/150‑1 (MQ=255)
                                                                                                                           ttCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGgt                           >  1:3994763/1‑150 (MQ=255)
                                                                                                                            tCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGgtg                          >  2:157780/1‑150 (MQ=255)
                                                                                                                               gATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgc                       >  1:3255703/1‑150 (MQ=255)
                                                                                                                                aTCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTATGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgcg                      >  1:2786459/1‑150 (MQ=255)
                                                                                                                                aTCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgcg                      >  1:1126588/1‑150 (MQ=255)
                                                                                                                                 tCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgcgc                     >  1:2658672/1‑150 (MQ=255)
                                                                                                                                 tCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCAAACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTgcgc                     >  1:1940095/1‑150 (MQ=255)
                                                                                                                                  cAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCa                    >  2:422440/1‑150 (MQ=255)
                                                                                                                                     cAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACt                 >  1:2482840/1‑150 (MQ=255)
                                                                                                                                      aGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAGGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTg                >  2:2073735/1‑150 (MQ=255)
                                                                                                                                       gCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTgg               >  1:4177586/1‑150 (MQ=255)
                                                                                                                                        cGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGc              <  2:1916132/150‑1 (MQ=255)
                                                                                                                                              tCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAgg        >  2:840022/1‑150 (MQ=255)
                                                                                                                                                 aTTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAggcgg     >  1:2832297/1‑150 (MQ=255)
                                                                                                                                                   tAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCt   <  2:2177779/150‑1 (MQ=255)
                                                                                                                                                    aaGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTg  >  1:713479/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTACGCCAGTCCTAAGGCGTCGTACTGCACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGATGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCCTTAGGCGGCTG  >  NC_000913/2828388‑2828685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: