BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,054,888	A→T	8.1%	V186E (GTA→GAA)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,054,888	0	A	T	8.1%	729.3 / 13.0	237	V186E (GTA→GAA)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand): ref base A (82/131); new base T (19/2); total (104/133)
Fisher's exact test for biased strand distribution p-value = 3.46e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAG  >  NC_000913/3054740‑3055036

aCTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAc                                                                                                                                                     >  1:2929097/1‑150 (MQ=255)

aCTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAc                                                                                                                                                     <  1:168440/150‑1 (MQ=255)

  ttCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACCCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACtt                                                                                                                                                   <  1:3219078/150‑1 (MQ=255)

    cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCt                                                                                                                                                 <  2:549043/150‑1 (MQ=255)

    cTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCt                                                                                                                                                 <  2:1969636/150‑1 (MQ=255)

       gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTGAGTGTAGAGTCGGTTTTTGTACttattgc                                                                                                                                              >  2:499203/1‑145 (MQ=255)

       gTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCAGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTGCGTCTGgc                                                                                                                                              >  1:4056596/1‑150 (MQ=255)

          cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGcgcg                                                                                                                                           <  1:2654705/150‑1 (MQ=255)

          cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGcgcg                                                                                                                                           <  2:4057063/150‑1 (MQ=255)

             caAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTCTGGCGcggtc                                                                                                                                        >  1:227868/1‑150 (MQ=255)

              aaaaaTCAGCGCGGCGCTGCCGGGGTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggtcg                                                                                                                                       <  1:1585235/150‑1 (MQ=255)

               acaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCTGGGCCTGACGGCGACGCTGAAACTCTTGCCGTGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGcggtcgg                                                                                                                                      <  1:3237584/148‑1 (MQ=255)

                aaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTAAGGCGcgggcggt                                                                                                                                     >  2:1305753/1‑150 (MQ=255)

                  aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                   <  2:3061557/150‑1 (MQ=255)

                  aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                   <  1:2760786/150‑1 (MQ=255)

                    cAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCg                                                                                                                                 <  1:3329512/150‑1 (MQ=255)

                       cgcgGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                              <  2:2301881/150‑1 (MQ=255)

                       cgcgGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                              <  2:3897754/150‑1 (MQ=255)

                       cgcgGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTAACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                              <  2:3739115/150‑1 (MQ=255)

                         cggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATgt                                                                                                                            >  2:2433370/1‑149 (MQ=255)

                             gcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTCTTGCGCGGTGGGTTGGGGATGAGtta                                                                                                                        >  2:3171245/1‑148 (MQ=255)

                             gcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTACTTGCGCCGTAGGTTGCGGAGGTGTaa                                                                                                                        >  1:3952720/1‑150 (MQ=255)

                              cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaaa                                                                                                                       <  2:1374239/150‑1 (MQ=255)

                               tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                      <  1:2913855/150‑1 (MQ=255)

                               tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                      <  2:1181337/150‑1 (MQ=255)

                               tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                      <  2:1039265/150‑1 (MQ=255)

                                 ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTATTGCGCGGGCGGGTGGGGAGGGGTAAAggg                                                                                                                    >  1:391164/1‑149 (MQ=255)

                                 ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAgatctggt                                                                                                                    <  2:3804006/150‑8 (MQ=255)

                                    ggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTATGGCGCGGGGGGGTGCGGGTGAGTTAATgtgtg                                                                                                                 >  1:3779971/1‑149 (MQ=255)

                                     gTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGGTGCGGGTGAGTAAAGGTGTCg                                                                                                                >  1:134160/1‑150 (MQ=255)

                                        gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTGTGGCGCGGTCGGTTGCGGGTGGTTAAAGGTGtggtgg                                                                                                             >  2:3306894/1‑150 (MQ=255)

                                        gCATGTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                             <  2:977392/150‑1 (MQ=255)

                                         cATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGTGTCTGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGTTGAGTAAAGGTGTCGTGGc                                                                                                            <  2:2646754/150‑1 (MQ=255)

                                           tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                                          <  2:3943339/150‑1 (MQ=255)

                                           tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTGTGGGGCGGTCGGGTGGGGGTGGGTAAAGGTGTGGTGGCag                                                                                                          >  1:1125041/1‑149 (MQ=255)

                                              gCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTAAGGGGCGGTGGGTGTCGGATGGGTTAAGGTGTCGTGGCCTaaa                                                                                                       >  1:1625279/1‑149 (MQ=255)

                                              gCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCGCGGGCGGGTGCGGGGGGGTAAAGGTGTCGGGGCCTAAc                                                                                                       >  1:2794407/1‑150 (MQ=255)

                                                tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTCTTGGGCGGTGGGTTGCGGATGTGTTAATGTTTCGTGGCATaaaac                                                                                                     >  1:511006/1‑147 (MQ=255)

                                                tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTCTTTCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                     >  2:1740542/1‑150 (MQ=255)

                                                tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTCTGGCGCGGTCGGGTGCGGGTGTGTAAAGGTGTCGGGGCATAACAg                                                                                                     >  1:828151/1‑150 (MQ=255)

                                                 ccaccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgc                                                                                                    >  2:2919720/1‑150 (MQ=255)

                                                  caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                   <  2:1431259/150‑1 (MQ=255)

                                                    ccaGGGCCTGACGGCGACGCTGACACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                 <  2:3140254/150‑1 (MQ=255)

                                                      aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                                               >  2:3278526/1‑150 (MQ=255)

                                                            tGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTATTGCGCGGTGGGTTTCGGATGAGTAAAGGTTTCGTTGCATAACAGCGCGGCAACACg                                                                                         >  2:2664529/1‑150 (MQ=255)

                                                             gACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTGTTTTGTGGGGGTGGGTTGGGGTTGTGTATAGGTGTGGTGGGATTAAAGGGCGGGAACACGa                                                                                        >  2:2254143/1‑150 (MQ=255)

                                                             gACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGa                                                                                        <  1:1531188/150‑1 (MQ=255)

                                                             gACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTAGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGa                                                                                        <  2:2174756/150‑1 (MQ=255)

                                                               cGGCTACGCTGAAACTATTTCCGGTTTATCCCAATCATAACAATCTCCTTACGTTTTTTTTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTAGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAAc                                                                                      <  1:1743583/150‑1 (MQ=255)

                                                                  cGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTa                                                                                   <  1:2541520/150‑1 (MQ=255)

                                                                    aCGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTAtt                                                                                 <  2:2284501/150‑1 (MQ=255)

                                                                     cGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTc                                                                                <  2:2852290/150‑1 (MQ=255)

                                                                     cGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTc                                                                                <  1:552521/150‑1 (MQ=255)

                                                                       cTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCga                                                                              <  1:2159170/150‑1 (MQ=255)

                                                                       cTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTCGGGTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAAAACGAAAGTATTCga                                                                              >  2:251583/1‑150 (MQ=255)

                                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgat                                                                             <  2:774033/150‑1 (MQ=255)

                                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgat                                                                             <  1:1065463/150‑1 (MQ=255)

                                                                        tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgac                                                                             <  2:4275965/150‑2 (MQ=255)

                                                                           aaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCCGTGGGGTGCGGGGGAGGAAAGGTGTCGTGGCATAACAACGCCGCAACACGAACGGATTAgatgat                                                                          >  1:2346620/1‑150 (MQ=255)

                                                                              tctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTATGGCGCGGTGGGTTGCGGATGTGTTAAGGTGTCGTGGCATAACAGCGCGGCAAAACGAAAGTATTAGATGAtatc                                                                       >  2:2478343/1‑150 (MQ=255)

                                                                                   ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCaa                                                                  <  1:3732475/150‑1 (MQ=255)

                                                                                    cGGGATATCTCTCTCATAACACTCTCCTTACGTTTTTTGTTTTTTGTGTAGAGTCGGTTTTTGTTCGTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATACCAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAg                                                                 <  1:3202845/150‑1 (MQ=255)

                                                                                    cGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTCGGTTGCGGGGGGGTGAAGGTGTCGGGGCATAGAAGCGCGGCCACACGAACGGAGTCGATGATCTCTTCAAg                                                                 >  2:203293/1‑150 (MQ=255)

                                                                                    cGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTCTTATGGGGCGGGCGGTTGCGGGTGGGTGAAGGGTTGGTGGGAGAACAGCGCGGCAACACCAACCGATTAGATGATCTCTTCAAg                                                                 >  1:163480/1‑150 (MQ=255)

                                                                                         tatCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACa                                                            <  1:4318748/150‑1 (MQ=255)

                                                                                           tctcACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACAtt                                                          <  1:4213971/150‑1 (MQ=255)

                                                                                            ctcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTGCGCGGTCGGGTGCGGATGAGTAAAGGTGGCGTGGCATAAAAGCGCCGCCACACCAAAGTATTAGATGATATCGTAAAGCGACAatt                                                         >  2:1650842/1‑150 (MQ=255)

                                                                                             tcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAAAGCGCGGCAACACGAACGGATTCGATGATAACTTCAAGCGACAatta                                                        >  2:2107051/1‑149 (MQ=255)

                                                                                             tcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGCGGTCGGGTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACCAACGTATTAGATTATCACATCAAGCGACAttta                                                        >  2:1774238/1‑149 (MQ=255)

                                                                                             tcactcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTc                                                        >  2:2854724/1‑150 (MQ=255)

                                                                                               actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                      <  2:1830974/150‑1 (MQ=255)

                                                                                                        acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTGTTGAGCGGTCGGTTGCGGATGTGTAAAGGTGTGGTGGCATAAAAGCGCGGCAACACGAACGTATTAGGTGATCTATTCAATCGACATTTCAAGCTCTTGCt                                             >  2:3149742/1‑150 (MQ=255)

                                                                                                        acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                                             <  1:3091118/150‑1 (MQ=255)

                                                                                                            tcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtc                                         <  2:1125041/150‑1 (MQ=255)

                                                                                                              cTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCCGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAAAACAGCGCGGCAACACGAACGTATTAGATGAAATCTTCAATCGACAATTCAAGCAAATACTGAtctt                                       >  2:2922891/1‑150 (MQ=255)

                                                                                                               ttACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttc                                      <  2:2584029/150‑1 (MQ=255)

                                                                                                                    ttttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                 <  2:3615864/150‑1 (MQ=255)

                                                                                                                    ttttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTCGGTTGCGGTTGTGTAAAGGTGTCGGGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACAATTGAAGCTCATCCTGAtcttcgtctt                                 >  2:1342363/1‑150 (MQ=255)

                                                                                                                     tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                <  1:66819/150‑1 (MQ=255)

                                                                                                                     tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                <  2:2929097/150‑1 (MQ=255)

                                                                                                                     tttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                <  2:3341295/150‑1 (MQ=255)

                                                                                                                      ttttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCACGGCAACACGAACGTATTCGATGATCTCTTCAAGCTACATTTCAAGCTCTTCCTGAtcttcgtcttcg                               <  2:2709931/150‑1 (MQ=255)

                                                                                                                        ttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCttcgtcttcgtc                             <  1:3588206/150‑1 (MQ=255)

                                                                                                                        ttGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCttcgtcttcgtc                             <  1:986878/150‑1 (MQ=255)

                                                                                                                          gTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt                           <  1:3093909/150‑1 (MQ=255)

                                                                                                                           tttttAGTGTAGAGTCGGTTTTTTTAATTATGGCGCCGGCGGGTGCGGCGGTGGAAAGGTGTCGGGGCCAAAAAGCCCGGCACCACGAACCGATTAGATGAAAACTTCAAGCGCCAATTCAAGCGATGACGGATGTTCGTCTTGGTCGTa                          >  2:1877720/1‑150 (MQ=255)

                                                                                                                           tttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTa                          <  2:1612748/150‑1 (MQ=255)

                                                                                                                                   gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGtt                  <  1:3401379/150‑1 (MQ=255)

                                                                                                                                         tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa            >  1:2491461/1‑150 (MQ=255)

                                                                                                                                          cGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAAt           <  1:3763027/150‑1 (MQ=255)

                                                                                                                                           ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATg          <  1:336517/150‑1 (MQ=255)

                                                                                                                                           ggTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATg          <  1:1853421/150‑1 (MQ=255)

                                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt         >  1:1614700/1‑150 (MQ=255)

                                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt         >  2:233237/1‑150 (MQ=255)

                                                                                                                                            gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt         >  1:868267/1‑150 (MQ=255)

                                                                                                                                              ttttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTa       <  2:1335843/150‑1 (MQ=255)

                                                                                                                                              ttttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTa       <  2:899500/150‑1 (MQ=255)

                                                                                                                                               tttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTAc      >  2:4343404/1‑150 (MQ=255)

                                                                                                                                               tttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTAc      <  2:687310/150‑1 (MQ=255)

                                                                                                                                                ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACg     >  2:1533555/1‑150 (MQ=255)

ACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAG  >  NC_000913/3054740‑3055036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`