Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,212 C→G 5.0% intergenic (‑184/+246) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,2120CG 5.0% 954.6 / 2.1 332intergenic (‑184/+246)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base C (162/141);  new base G (3/19);  total (172/160)
Fisher's exact test for biased strand distribution p-value = 2.67e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

ATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGC  >  NC_000913/3392063‑3392361
                                                                                                                                                      |                                                                                                                                                     
aTTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                                                                       <  2:1772162/150‑1 (MQ=255)
 ttttttCCGGCTGCCGGTTGCGGCGTGAACGCCCTATCCAGCCTACGTTTTTGTTCCGTTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCg                                                                                                                                                      <  1:132941/150‑1 (MQ=17)
  tttttCCGGCGGCCGTAGGCGGCGTGACCGCCCTATCCAGCCTACGTTTTTGTCCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGCCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGt                                                                                                                                                     <  1:620784/150‑1 (MQ=17)
        cggcggCCGGTGGGGGCGTGAACGCCCTACCCCGCCTCGGGTTTTGTGCGGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAg                                                                                                                                               <  1:2626380/150‑1 (MQ=14)
         ggCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAgg                                                                                                                                              <  2:931545/150‑1 (MQ=255)
          gCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                                                                                             <  1:2709070/150‑1 (MQ=255)
            tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                                           >  1:1454537/1‑150 (MQ=255)
              ccGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                                                                         >  2:3606638/1‑150 (MQ=255)
               cggtggcggcgTTAACGCCCTACCCACCCTAGGGTTATGTTCCGGTTTTTTCGCCTTATAATAGGCACCGGGTCGCATCCGCAAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                                                                        <  1:3293160/145‑1 (MQ=14)
               cGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                                                                        >  2:3106764/1‑150 (MQ=255)
                ggATGCGGCGTGAACGCCCTATCCAGCATAGGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                                                                                                                       <  1:3418798/150‑1 (MQ=255)
                 gATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATaa                                                                                                                                      >  2:728315/1‑150 (MQ=255)
                      ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                                                                                                                 >  2:2389635/1‑150 (MQ=255)
                      ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGCCCTGATAAGACGc                                                                                                                                 >  1:1215751/1‑150 (MQ=255)
                      ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGc                                                                                                                                 >  1:767196/1‑150 (MQ=255)
                         gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                                                                                                              <  1:3282392/150‑1 (MQ=255)
                              cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                         <  1:1604676/150‑1 (MQ=255)
                              cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                         <  1:639131/150‑1 (MQ=255)
                              cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                         <  2:2316037/150‑1 (MQ=255)
                                cccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                                       >  1:3806172/1‑150 (MQ=255)
                                 ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                                                      >  1:722725/1‑150 (MQ=255)
                                  cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                                                     <  2:195736/150‑1 (MQ=255)
                                  cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                                                     >  1:2016937/1‑150 (MQ=255)
                                  cTAGCCAGCCTCCGGTTATGGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGTCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                                                     <  2:2253941/150‑1 (MQ=255)
                                   tATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                                                                    >  2:279539/1‑150 (MQ=255)
                                      ccAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                 <  2:3049278/150‑1 (MQ=40)
                                      ccAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                 <  1:1607856/150‑1 (MQ=40)
                                       cAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGc                                                                                                                >  1:1672766/1‑150 (MQ=39)
                                       cAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGc                                                                                                                >  2:559362/1‑150 (MQ=39)
                                       cAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGATGTATGCCTGATAAGACGCACAGAGTCGCCTCAgtc                                                                                                                >  2:440075/1‑148 (MQ=11)
                                               ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                                        >  1:2921223/1‑150 (MQ=37)
                                                 ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCCTATCCGACCTACGGTTATGTTCAGTATCTAGGCCAGATAAGACGCACATCCACGCATCACGCAACGGCTGcc                                                                                                      >  2:3822750/1‑150 (MQ=11)
                                                 ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCATATCCGACCTACGGTTATGTTCCGTGTCTATGCCTGATAACACCCACACCGGCGCAACAGGCACCCGCTGcc                                                                                                      >  2:94546/1‑150 (MQ=11)
                                                     gTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCCGGCAGCCGTTGCCTGAt                                                                                                  <  2:2671304/150‑1 (MQ=11)
                                                        ccGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                                               >  1:140257/1‑150 (MQ=34)
                                                        ccGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                                               >  1:1006716/1‑150 (MQ=34)
                                                          ggTTTTTTGGCCTGATAAGACGCACAGCGTCGCATCTGGAAAGGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                                             <  2:1256515/150‑1 (MQ=11)
                                                            tttGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGt                                                                                           <  2:3913602/150‑1 (MQ=32)
                                                                  ggCCTTATAAGCCGCCCTGCGTGGCATCTGGCAAGGGCTGTCGTATGGGGGTTAAACGCCTTATCCGACCTACGGTTTTGTTCCGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCCGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                                                     <  2:1130563/150‑1 (MQ=11)
                                                                    ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                                                   >  1:2470278/1‑150 (MQ=35)
                                                                       gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTAt                                                                                >  1:2368504/1‑150 (MQ=37)
                                                                       gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTGTGTAGGCCTGATGAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGAGGCGGCGGAAACGCCttct                                                                                >  1:1107031/1‑148 (MQ=11)
                                                                        ttacgACGCACAGCGTCGCATATTGCAAGGGCTGTCGGTTGCGGGTTAAACGCCTTAACCGACCTACGTTTTTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc                                                                               <  2:1535804/146‑1 (MQ=11)
                                                                        aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc                                                                               <  2:3258195/150‑1 (MQ=37)
                                                                         tAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATcc                                                                              >  1:4191663/1‑150 (MQ=37)
                                                                         tAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATcc                                                                              >  2:451869/1‑150 (MQ=37)
                                                                             aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                          >  1:3767130/1‑150 (MQ=37)
                                                                             aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCCTATCCGAcc                                                                          >  1:1837287/1‑150 (MQ=17)
                                                                               gCACAGTGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                                        >  2:3774212/1‑150 (MQ=37)
                                                                                     cGTCGAATCCGGCACGGGCTGGCGGATGCGGCGTAAACGCCTTACCCGACCTTCGGTTTTGTTCCGTTTGTTGGCATGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa                                                                  <  1:2888875/150‑1 (MQ=11)
                                                                                       tCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                >  1:2226905/1‑150 (MQ=37)
                                                                                              aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGtt                                                         >  2:3240088/1‑150 (MQ=40)
                                                                                              aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTATGTTCCGtt                                                         >  1:3949409/1‑150 (MQ=38)
                                                                                                  cccGGCTGTGGGTTGCGGGGTAACCGCCTTTGCCGCCCTCGGTTTTGGTGCGGTTTGTGGGCCTGATACGACGCACCGCGTGGCATCCGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                     <  1:930804/148‑1 (MQ=2)
                                                                                                     ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                  >  2:2075521/1‑150 (MQ=255)
                                                                                                     ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGATATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCAACGGGTATGTTCCGTTTGTAGGc                                                  >  1:2598715/1‑150 (MQ=255)
                                                                                                     ggCTGCCGGAGGCGGCGTAACCCCCTTACCCGACATACGGTATTGTTCCGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                  <  2:1506793/150‑1 (MQ=14)
                                                                                                      ggtGTCGGTTGGGGCTAAACCGCCTTATCCGACCTAGGTTTTTGTTCGGTTTGTTGGCCTGATAAGACGAACTGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                 <  1:3851497/148‑1 (MQ=14)
                                                                                                      gCTGTCGTATGCGGCTTAAACGCCTTATCCGACCTACTTTTATGTTCCGTTTGTAGGCCTGTTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                 <  2:3144127/150‑1 (MQ=14)
                                                                                                       gtgggggAGGCGGCGTAAACGCCTTAGCCGACCTCCGGTTATGGTCCGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                <  1:844748/145‑1 (MQ=14)
                                                                                                       cTGTCGGATGCGGCGTAACCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACTGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                <  2:3683513/150‑1 (MQ=255)
                                                                                                       cTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                >  1:4105678/1‑150 (MQ=255)
                                                                                                         gTCGGTTGCGGCGTAAACGCCTTACCCGACCTAGTTTTTTGTTCGTTTTGTTGGCGTTATACGACGCACCGCTTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                              <  1:2065538/150‑1 (MQ=14)
                                                                                                         gTCGGTGGCGGCGTAACCGCCTTACCCGACCTCGGGTTTTTTTCGGTTTGTCGGCCTTATAAGCCGCACCGCGTCGCATCCGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                              <  1:1572245/150‑1 (MQ=14)
                                                                                                         gTCGGATAAGGCGTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                              >  2:1393778/1‑150 (MQ=14)
                                                                                                            ggtggcggcgTACCCGCGTTAGCCGCCCTCGGGTTTGGGTCCGTTTGTTGGCCTGATAAGACGCCCCGCGGCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                           <  2:4131183/146‑1 (MQ=11)
                                                                                                            ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                           >  2:3461706/1‑150 (MQ=255)
                                                                                                              aTGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                         <  2:2429804/150‑1 (MQ=255)
                                                                                                              aTGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                         >  1:2498118/1‑150 (MQ=255)
                                                                                                                 cggcgTAAACGCCTTATCCGACCTACGGTTTTGTTCCGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                      <  1:3510372/150‑1 (MQ=255)
                                                                                                                  ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGca                                     >  2:4015483/1‑150 (MQ=255)
                                                                                                                     gtaacggCCTTATCCGACCTACGTTTTTGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAg                                  <  2:640449/144‑1 (MQ=14)
                                                                                                                     gTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCCGTTTGTAGGCCTGATAAGACGCACAg                                  >  2:4273711/1‑150 (MQ=255)
                                                                                                                        aaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATAAGACGCACAgaga                               >  2:3525020/1‑147 (MQ=255)
                                                                                                                         ccGCCTTAGCCGACCTACGTTTTTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                              <  1:2840301/149‑1 (MQ=255)
                                                                                                                         aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                              >  2:1683222/1‑150 (MQ=255)
                                                                                                                          cGCCTTATCCTCCCTACGGTTTTGTTCCTTTTGTAGGCCTTATACGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                             <  1:3653179/150‑1 (MQ=11)
                                                                                                                          cGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                             <  2:4296846/150‑1 (MQ=255)
                                                                                                                             cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGACGCAt                          >  2:2907801/1‑150 (MQ=255)
                                                                                                                              ttATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                         >  1:3611228/1‑150 (MQ=255)
                                                                                                                              ttATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                         >  2:3330825/1‑150 (MQ=255)
                                                                                                                               tagccGCCCTCCGGTTATGTTCCGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                        <  1:347567/147‑1 (MQ=255)
                                                                                                                                agccGCCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                       <  2:2315122/148‑1 (MQ=37)
                                                                                                                                aTCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACAg                       >  2:1360146/1‑150 (MQ=255)
                                                                                                                                 tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                      >  2:2720793/1‑150 (MQ=255)
                                                                                                                                   cGACCTCCGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                    <  1:3560212/150‑1 (MQ=255)
                                                                                                                                   cGACCTACGTTTATTTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                    <  1:3034203/150‑1 (MQ=255)
                                                                                                                                   cGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                    >  2:2900079/1‑150 (MQ=38)
                                                                                                                                   cGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                    >  2:1218041/1‑150 (MQ=38)
                                                                                                                                    gACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCac                   >  1:3967341/1‑149 (MQ=38)
                                                                                                                                    gACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCaa                   >  1:1972714/1‑150 (MQ=38)
                                                                                                                                     aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                  >  1:4333567/1‑150 (MQ=37)
                                                                                                                                          gggTTATGTTCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTTTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg             <  2:2566953/149‑1 (MQ=255)
                                                                                                                                                tttttCGGTTTGTAGGCATGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa        <  2:1060783/147‑1 (MQ=9)
                                                                                                                                                tGTTCGGTTTGTTGGCCTGTAACGACGCAACGCTTCGCATACGGAACCGGCTGCCGTAGGCGGCGTAAACGCCTTACCCGACCTACGTTTTTTTCCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt       <  2:2589617/150‑1 (MQ=11)
                                                                                                                                                tGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt       <  2:2457645/150‑1 (MQ=35)
                                                                                                                                                   ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc     >  2:4222509/1‑150 (MQ=35)
                                                                                                                                                    tCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAAGCGGCGTAAACGCCTAATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGTCTCATCAGGCAACGGCTGCCGCATgcg    >  1:316166/1‑150 (MQ=11)
                                                                                                                                                      cGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc  >  2:1746183/1‑150 (MQ=32)
                                                                                                                                                      cGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATGAGACGCACAGAGACGCATCAGGCGACGGCTGCCGCAggcggc  >  2:2523411/1‑150 (MQ=11)
                                                                                                                                                      |                                                                                                                                                     
ATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGC  >  NC_000913/3392063‑3392361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: