Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,482,3250GA100.0% 1001.7 / NA 261R346H (CGC→CAC) yheSABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (118/143);  total (118/143)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCT  >  NC_000913/3482178‑3482470
                                                                                                                                                   |                                                                                                                                                 
 gTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACaa                                                                                                                                                <  2:917198/150‑1 (MQ=255)
 gTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACaa                                                                                                                                                <  1:812666/150‑1 (MQ=255)
      gCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcg                                                                                                                                           >  1:3210074/1‑150 (MQ=255)
        tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                         >  1:2536010/1‑150 (MQ=255)
         ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTACACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                        <  2:607085/150‑1 (MQ=255)
          tAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCggg                                                                                                                                       <  1:2414027/150‑1 (MQ=255)
             cTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                    <  2:727733/150‑1 (MQ=255)
             cTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                    >  1:1687901/1‑150 (MQ=255)
              ttCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaaa                                                                                                                                   <  2:4247082/150‑1 (MQ=255)
               tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                  <  2:1297112/150‑1 (MQ=255)
               tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                  <  1:2118162/150‑1 (MQ=255)
               tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                  <  1:1880762/150‑1 (MQ=255)
                ccGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATc                                                                                                                                 <  2:3311022/150‑1 (MQ=255)
                  gcgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGa                                                                                                                               <  2:3885030/150‑1 (MQ=255)
                   cgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAc                                                                                                                              <  1:2660906/150‑1 (MQ=255)
                     cgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACAt                                                                                                                            <  1:653844/150‑1 (MQ=255)
                      gcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACAtt                                                                                                                           <  1:3079172/150‑1 (MQ=255)
                        cGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTaa                                                                                                                         >  2:3263923/1‑150 (MQ=255)
                        cGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTaa                                                                                                                         >  2:2249934/1‑150 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                       >  1:2455594/1‑150 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                       <  1:3417296/150‑1 (MQ=255)
                            aaGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCaa                                                                                                                     >  2:2502700/1‑150 (MQ=255)
                              gCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAAc                                                                                                                   <  2:1735249/150‑1 (MQ=255)
                               ccTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTCTGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACt                                                                                                                  >  1:2378294/1‑150 (MQ=255)
                               ccTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACt                                                                                                                  >  2:4077507/1‑150 (MQ=255)
                                 tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                >  1:4165766/1‑150 (MQ=255)
                                  gCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTCTTGGTCTGTTAGGCCACAATGGCGCGGGTCCATCGACATTAATCAAACTCtt                                                                                                               >  2:3866139/1‑150 (MQ=255)
                                  gCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGtt                                                                                                               <  2:888376/150‑1 (MQ=255)
                                  gCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGtt                                                                                                               <  2:901684/150‑1 (MQ=255)
                                  gCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGtt                                                                                                               >  2:477964/1‑150 (MQ=255)
                                    cAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAg                                                                                                             <  1:734856/150‑1 (MQ=255)
                                    cAAATCCGGTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAg                                                                                                             <  1:78233/150‑1 (MQ=255)
                                     aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                            <  2:3043890/150‑1 (MQ=255)
                                     aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                            <  2:1859825/150‑1 (MQ=255)
                                      aaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGcc                                                                                                           >  1:3361594/1‑150 (MQ=255)
                                       aTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCg                                                                                                          <  2:3704736/150‑1 (MQ=255)
                                        tCCGGTACTGAAGCTGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCgg                                                                                                         <  1:2427524/150‑1 (MQ=255)
                                          cGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTg                                                                                                       >  2:550042/1‑150 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                      <  1:110933/150‑1 (MQ=255)
                                               cTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTAGTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACAGTAATCAAACTGTTAGCCGGTGAACtg                                                                                                  >  2:2188421/1‑149 (MQ=255)
                                                tGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTg                                                                                                 >  2:1542266/1‑150 (MQ=255)
                                                  aaGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgcg                                                                                               >  1:3505542/1‑150 (MQ=255)
                                                     aTGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCa                                                                                            >  2:1043286/1‑150 (MQ=255)
                                                       ggAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGt                                                                                          >  1:120532/1‑150 (MQ=255)
                                                        gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                         >  2:2477628/1‑150 (MQ=255)
                                                        gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                         >  1:2099065/1‑150 (MQ=255)
                                                         aaaaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTATTCAAACTGTTAGCCGGTGAACTTGCGCCAGTCa                                                                                        >  2:3610694/1‑150 (MQ=255)
                                                           aaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGc                                                                                      >  1:1519977/1‑150 (MQ=255)
                                                            aaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCg                                                                                     <  2:125540/150‑1 (MQ=255)
                                                              gTCAGCGCGGGCTATGGCTATCGCTTTTTTCTCGACTCGATTATACTTAACCTGGTTCCCGGCTAGCTTATTGGTCTGTTAGGCCACAATGGCGCGGTTAAATCGACATTTATCAATCTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGt                                                                                   <  2:1481710/150‑1 (MQ=255)
                                                               tCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTg                                                                                  >  1:3803936/1‑150 (MQ=255)
                                                                     cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                            <  1:2807679/150‑1 (MQ=255)
                                                                       ggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGt                                                                          <  2:1001591/150‑1 (MQ=255)
                                                                       ggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGt                                                                          <  1:3862729/150‑1 (MQ=255)
                                                                         cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                        >  1:410530/1‑150 (MQ=255)
                                                                          tATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                       >  1:3292947/1‑150 (MQ=255)
                                                                           aTGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTgg                                                                      <  1:270633/150‑1 (MQ=255)
                                                                           aTGGCGATCGCATTAGTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTgg                                                                      <  2:2613354/150‑1 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                 >  2:3678134/1‑150 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                 >  2:1149545/1‑150 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                 <  1:3002793/150‑1 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                 >  2:3347778/1‑150 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                 <  2:4342051/150‑1 (MQ=255)
                                                                                    gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                             <  2:1975978/150‑1 (MQ=255)
                                                                                     cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                            >  2:4196013/1‑150 (MQ=255)
                                                                                      attattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATc                                                           <  1:1423021/150‑1 (MQ=255)
                                                                                      attataCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACAGTAAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAACGGATc                                                           >  2:1584416/1‑150 (MQ=255)
                                                                                          ttCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAAc                                                       >  1:1036916/1‑150 (MQ=255)
                                                                                               gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                  <  1:1013772/150‑1 (MQ=255)
                                                                                                  tcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAc                                               >  1:1850102/1‑150 (MQ=255)
                                                                                                   cgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACt                                              <  1:2036976/150‑1 (MQ=255)
                                                                                                          aCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCa                                       >  2:2062834/1‑150 (MQ=255)
                                                                                                          aCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCa                                       >  2:2885294/1‑150 (MQ=255)
                                                                                                           cTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAg                                      <  1:3637616/150‑1 (MQ=255)
                                                                                                            tGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGc                                     >  2:2699157/1‑150 (MQ=255)
                                                                                                               aCCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATc                                  <  2:2455539/150‑1 (MQ=255)
                                                                                                                ccTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCa                                 >  1:3918946/1‑150 (MQ=255)
                                                                                                                  tGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAAc                               <  2:2031912/150‑1 (MQ=255)
                                                                                                                   ggTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACt                              >  1:1927965/1‑150 (MQ=255)
                                                                                                                   ggTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACt                              >  1:2969094/1‑150 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtt                             <  1:674698/150‑1 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtt                             <  1:4254859/150‑1 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtc                             <  1:1297220/150‑2 (MQ=255)
                                                                                                                     tGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTg                            >  1:1791911/1‑150 (MQ=255)
                                                                                                                       cccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGaa                          <  2:2784247/150‑1 (MQ=255)
                                                                                                                       cccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAACCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGaa                          <  2:4063543/150‑1 (MQ=255)
                                                                                                                        ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                         <  2:3361594/150‑1 (MQ=255)
                                                                                                                         cGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATa                        >  2:3831733/1‑150 (MQ=255)
                                                                                                                          ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCTCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                       >  1:3425564/1‑150 (MQ=255)
                                                                                                                          ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                       >  1:2570246/1‑150 (MQ=255)
                                                                                                                            cTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCt                     <  2:4191042/150‑1 (MQ=255)
                                                                                                                              cgcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgc                   <  1:3110741/150‑1 (MQ=255)
                                                                                                                               gcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcg                  >  1:382592/1‑150 (MQ=255)
                                                                                                                                   aTTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGcc              >  1:4026160/1‑150 (MQ=255)
                                                                                                                                        tCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacga         >  1:135740/1‑150 (MQ=255)
                                                                                                                                        tCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacga         >  1:135643/1‑150 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt       <  2:4173850/150‑1 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt       <  2:2414498/150‑1 (MQ=255)
                                                                                                                                            ttAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCa     >  1:3984832/1‑150 (MQ=255)
                                                                                                                                               ggCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGACTCACCt  >  1:3144390/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCT  >  NC_000913/3482178‑3482470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: