Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,539,815 A→T 5.3% intergenic (+109/‑348) yhgF → / → feoA putative transcriptional accessory protein/ferrous iron transporter, protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,539,8150AT 5.3% 938.2 / 32.3 263intergenic (+109/‑348)yhgF/feoAputative transcriptional accessory protein/ferrous iron transporter, protein A
Reads supporting (aligned to +/- strand):  ref base A (128/118);  new base T (7/7);  total (138/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

Only 100 of 283 total aligned reads displayed.

TGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGAG  >  NC_000913/3539666‑3539964
                                                                                                                                                     |                                                                                                                                                     
tgatgGATGCGCGGGCGGCGGCAATGGGCAAAAAACTTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGaa                                                                                                                                                       <  1:1542858/150‑1 (MQ=255)
 gatggatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                                                                                                                                      <  2:3827078/150‑1 (MQ=255)
 gatggatgCGCTGGCGGCGGATATGGGCAAAAAACGTTAAACGCCCGTACTGGCATACGGTTCGAATTTTCTCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTTACATAAAAGTGCCGGAGAat                                                                                                                                                      <  2:1667601/150‑1 (MQ=255)
    ggatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGTATAtc                                                                                                                                                   >  2:2546956/1‑150 (MQ=255)
       tgCGCTGGCGGCGGCAATGGGCAAAAAACTTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                                                                                                                                <  1:2296788/150‑1 (MQ=255)
       tgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                                                                                                                                <  1:2212700/150‑1 (MQ=255)
          gcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc                                                                                                                                             >  1:2376737/1‑150 (MQ=255)
               cggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt                                                                                                                                        <  1:3076575/150‑1 (MQ=255)
                ggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttttt                                                                                                                                       >  2:2239585/1‑150 (MQ=255)
                  cggcAATGGGAAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                                                                                                                                     <  1:2808055/150‑1 (MQ=255)
                    gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTc                                                                                                                                   <  1:1419334/150‑1 (MQ=255)
                     cAATGGGCAAACAACTTAAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAACTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAATGCCGGAGATATTCTCCGGCACTTTTAtgtc                                                                                                                                  <  1:1867590/150‑4 (MQ=38)
                      attGGCCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGATATTCTCCGGCACTTTTAtgtca                                                                                                                                 <  2:3309696/148‑5 (MQ=38)
                      aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGATATTCTCCGGCACTTTTAtgtca                                                                                                                                 <  2:3557030/150‑5 (MQ=39)
                      aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCca                                                                                                                                 <  1:4295963/150‑1 (MQ=255)
                        tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                                                                                                                               <  2:3984596/150‑1 (MQ=255)
                        ggggCAAAAACGGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                                                                                                                               <  2:3670227/149‑1 (MQ=255)
                         gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                                                                                                                              >  1:2061216/1‑150 (MQ=255)
                         gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                                                                                                                              >  1:778735/1‑150 (MQ=255)
                          ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGc                                                                                                                             <  2:45065/150‑1 (MQ=255)
                          ccaaaaaaaCCTAAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACCAAATCGTAGGGCAGATAAGGCGTACACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGc                                                                                                                             <  1:1128852/147‑1 (MQ=255)
                              aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa                                                                                                                         >  2:2757464/1‑150 (MQ=255)
                              aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa                                                                                                                         <  2:1813412/150‑1 (MQ=255)
                              aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa                                                                                                                         >  2:927842/1‑150 (MQ=255)
                                aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACt                                                                                                                       >  1:433980/1‑150 (MQ=255)
                                  aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCa                                                                                                                     <  2:2049796/150‑1 (MQ=255)
                                    gTTAAACGCCCGTACTGGCCTCCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATTAAAGTGCCGGAGAATTTCTCCGGCATTTTTATTCCTCAGCCAAACTCata                                                                                                                   <  1:985345/150‑1 (MQ=255)
                                     ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGACTATCGCCGGCATGTTGATTCCACAGCCGAAGTCagag                                                                                                                  >  2:2687782/1‑147 (MQ=39)
                                        caCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAatat                                                                                                               <  2:2245710/149‑1 (MQ=255)
                                         aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAatata                                                                                                              >  1:3471282/1‑150 (MQ=255)
                                             ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTcc                                                                                                          >  2:1464513/1‑150 (MQ=255)
                                              cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCTGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCg                                                                                                         <  2:3175110/150‑1 (MQ=255)
                                              cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCg                                                                                                         <  2:2491403/150‑1 (MQ=255)
                                                 aCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCa                                                                                                      >  1:2270469/1‑150 (MQ=255)
                                                             ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatt                                                                                          >  2:1365002/1‑150 (MQ=255)
                                                             ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatt                                                                                          >  2:1879951/1‑150 (MQ=255)
                                                                gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttca                                                                                       <  2:114197/150‑1 (MQ=255)
                                                                 aaTTTGCACGACATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAACAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttcat                                                                                      <  2:1857660/150‑1 (MQ=255)
                                                                  aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttcatt                                                                                     <  2:3330808/150‑1 (MQ=255)
                                                                     tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaac                                                                                  <  1:2734925/150‑1 (MQ=255)
                                                                     tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaac                                                                                  <  1:3650806/150‑1 (MQ=255)
                                                                       cACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATACTATATTCCGGCAATATTTATCATTTCATTaacaa                                                                                >  2:2309701/1‑150 (MQ=255)
                                                                        aCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaacaac                                                                               <  1:1938837/150‑1 (MQ=255)
                                                                        aCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaacaac                                                                               >  1:3908547/1‑150 (MQ=255)
                                                                          gAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTg                                                                             >  2:582638/1‑150 (MQ=255)
                                                                          gAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTg                                                                             >  1:3286715/1‑150 (MQ=255)
                                                                            aaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaa                                                                           <  2:2637690/150‑1 (MQ=255)
                                                                            aaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaa                                                                           <  2:438117/150‑1 (MQ=255)
                                                                             aTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaaa                                                                          <  2:2385396/150‑1 (MQ=255)
                                                                              tCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAAc                                                                         <  1:4193450/150‑1 (MQ=255)
                                                                              tCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAAc                                                                         <  1:1359237/150‑1 (MQ=255)
                                                                                 tAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCtt                                                                      >  2:2815654/1‑150 (MQ=255)
                                                                                   gggCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaa                                                                    <  1:330463/150‑1 (MQ=255)
                                                                                    ggCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaat                                                                   >  1:2914440/1‑150 (MQ=255)
                                                                                     gCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaatt                                                                  >  2:1537585/1‑150 (MQ=255)
                                                                                       aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATGATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                >  2:3218260/1‑150 (MQ=255)
                                                                                       aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                >  1:117122/1‑150 (MQ=255)
                                                                                       aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                >  2:3112873/1‑150 (MQ=255)
                                                                                          tAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACa                                                             >  2:3693343/1‑150 (MQ=255)
                                                                                          tAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACa                                                             >  1:2449692/1‑150 (MQ=255)
                                                                                            aGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACAtt                                                           >  1:1208739/1‑150 (MQ=255)
                                                                                             ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                          >  1:1701464/1‑150 (MQ=255)
                                                                                             ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                          <  1:1258146/150‑1 (MQ=255)
                                                                                               cGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGc                                                        <  2:3471282/150‑1 (MQ=255)
                                                                                                gTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCATAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGcc                                                       >  2:28874/1‑150 (MQ=255)
                                                                                                gTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCATAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGcc                                                       >  2:2239830/1‑150 (MQ=255)
                                                                                                 ttCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCa                                                      <  1:1755935/150‑1 (MQ=255)
                                                                                                    aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTc                                                   >  2:538993/1‑150 (MQ=255)
                                                                                                    aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTc                                                   >  1:2975878/1‑150 (MQ=255)
                                                                                                       ccgcATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCgg                                                >  1:3379280/1‑150 (MQ=255)
                                                                                                              tGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTc                                         >  1:1586157/1‑150 (MQ=255)
                                                                                                                    cgaacgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTAtt                                   <  1:2463033/150‑1 (MQ=255)
                                                                                                                     gaccgCCTGGCCTGACATAACAGGGCCGGAGAATACCTCCGGCATGTTTATTCCACAGCCAAACTCATAATATCGTCCGGCAATATTTATCATTTCATGAACAACTGCAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                  <  1:2403145/147‑1 (MQ=255)
                                                                                                                     gaacgCCTTGCCTGACATACAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                  <  1:528974/150‑1 (MQ=255)
                                                                                                                     gaacgCCTTGCCTGACATAAATGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                  >  1:1462387/1‑150 (MQ=255)
                                                                                                                     gaacgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                  <  2:918956/150‑1 (MQ=255)
                                                                                                                     gaacgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACATTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                  >  2:4109960/1‑150 (MQ=255)
                                                                                                                       acgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGa                                >  1:922579/1‑150 (MQ=255)
                                                                                                                         gCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAt                              <  2:1228068/150‑1 (MQ=255)
                                                                                                                         gCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAt                              <  1:4032985/150‑1 (MQ=255)
                                                                                                                         gCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAt                              <  1:2261570/150‑1 (MQ=255)
                                                                                                                           cTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATta                            >  1:1089503/1‑150 (MQ=255)
                                                                                                                               ccTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTCTTCGAAGTATAtt                        >  1:257709/1‑150 (MQ=255)
                                                                                                                               ccTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATAtt                        >  1:257825/1‑150 (MQ=255)
                                                                                                                                cTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACAGTAGCCAGTCCGGGTAATGCACTATTCGAATTATAttt                       >  2:3374616/1‑150 (MQ=255)
                                                                                                                                 tGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATAtttt                      >  2:107394/1‑150 (MQ=255)
                                                                                                                                   gaaTAAAAATGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCg                    >  1:2755554/3‑150 (MQ=39)
                                                                                                                                     aTAAAAATGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCt                  >  1:3696895/1‑150 (MQ=255)
                                                                                                                                        aaaGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCg               >  1:3138308/1‑150 (MQ=255)
                                                                                                                                          aatGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAATCTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAATCCTTAATTAATCATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGat             >  1:2004542/3‑150 (MQ=255)
                                                                                                                                          aatGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGat             >  1:1761843/3‑150 (MQ=255)
                                                                                                                                            tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGatat           <  1:582638/150‑1 (MQ=255)
                                                                                                                                            tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGatat           <  1:3429480/150‑1 (MQ=255)
                                                                                                                                            tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGatat           >  1:3246507/1‑150 (MQ=255)
                                                                                                                                               cGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATATCATTAACAACTGAAACCTTAATTAAACATTAGACAGTCCGGGTAATTCACTATTCTAATTATATTTTCGCTGCGATATAAc        >  2:823671/1‑150 (MQ=255)
                                                                                                                                                 gagaATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCt      >  1:3959033/1‑150 (MQ=255)
                                                                                                                                                 gagaATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCt      <  1:3007572/150‑1 (MQ=255)
                                                                                                                                                    aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa   <  2:114518/150‑1 (MQ=255)
                                                                                                                                                    aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa   <  2:1603430/150‑1 (MQ=255)
                                                                                                                                                    aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa   <  2:2306184/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGAG  >  NC_000913/3539666‑3539964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: