Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,539,816 T→A 5.0% intergenic (+110/‑347) yhgF → / → feoA putative transcriptional accessory protein/ferrous iron transporter, protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,539,8160TA 5.0% 991.9 / 25.4 272intergenic (+110/‑347)yhgF/feoAputative transcriptional accessory protein/ferrous iron transporter, protein A
Reads supporting (aligned to +/- strand):  ref base T (131/127);  new base A (7/7);  total (138/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

GATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGAGC  >  NC_000913/3539667‑3539965
                                                                                                                                                     |                                                                                                                                                     
gatggatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                                                                                                                                       <  2:3827078/150‑1 (MQ=255)
gatggatgCGCTGGCGGCGGATATGGGCAAAAAACGTTAAACGCCCGTACTGGCATACGGTTCGAATTTTCTCGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTTACATAAAAGTGCCGGAGAat                                                                                                                                                       <  2:1667601/150‑1 (MQ=255)
gatggatgCGCTGGCGGCGGAAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                                                                                                                                       <  1:3868989/150‑1 (MQ=255)
   ggatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGTATAtc                                                                                                                                                    >  2:2546956/1‑150 (MQ=255)
   ggatgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                                                                                                                                    >  2:922857/1‑150 (MQ=255)
      tgCGCTGGCGGCGGCATTGGGCAAAATACTTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGATATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                                                                                                                                 <  2:3141250/150‑1 (MQ=255)
      tgCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc                                                                                                                                                 <  1:2212700/150‑1 (MQ=255)
             gcggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttt                                                                                                                                          >  1:2987948/1‑150 (MQ=255)
              cggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt                                                                                                                                         <  1:3076726/150‑1 (MQ=255)
               ggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttttt                                                                                                                                        >  2:2239585/1‑150 (MQ=255)
                 cggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                                                                                                                                      <  1:3011529/150‑1 (MQ=255)
                 cggcAATGGGAAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAt                                                                                                                                      <  1:2808055/150‑1 (MQ=255)
                   gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTc                                                                                                                                    <  1:1951130/150‑1 (MQ=255)
                   gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTc                                                                                                                                    <  1:1419334/150‑1 (MQ=255)
                    cAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAATGCCGGAGATATTCTCCGGCACTTTTAtgtc                                                                                                                                   <  2:581260/150‑4 (MQ=39)
                     attGGCCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGATATTCTCCGGCACTTTTAtgtca                                                                                                                                  <  2:3309696/148‑5 (MQ=38)
                     aaTGGGCAAACACCGTTAAACGCCCGTACTGGCCTACGGTTCGAATGTGCACGAACTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGATATTCTCCGGCACTTTTAtgtca                                                                                                                                  <  2:3556916/150‑5 (MQ=38)
                       tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                                                                                                                                <  2:3984591/150‑1 (MQ=255)
                       tGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCcaca                                                                                                                                >  2:1633481/1‑150 (MQ=255)
                        gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                                                                                                                               >  1:1907798/1‑150 (MQ=255)
                        gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                                                                                                                               >  1:2061216/1‑150 (MQ=255)
                        gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAg                                                                                                                               >  1:2965575/1‑150 (MQ=255)
                         ccaaaaaaaCCTAAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACCAAATCGTAGGGCAGATAAGGCGTACACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGc                                                                                                                              <  1:1128852/147‑1 (MQ=255)
                            aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaa                                                                                                                           >  1:329471/1‑150 (MQ=255)
                             aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa                                                                                                                          >  2:2757464/1‑150 (MQ=255)
                             aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCaaa                                                                                                                          <  2:1813412/150‑1 (MQ=255)
                                    ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGACTATCGCCGGCATGTTGATTCCACAGCCGAAGTCagag                                                                                                                   >  2:2687782/1‑147 (MQ=39)
                                       caCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAatat                                                                                                                <  2:2245710/149‑1 (MQ=255)
                                            ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTcc                                                                                                           >  2:1258146/1‑150 (MQ=255)
                                             cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCg                                                                                                          <  2:2491403/150‑1 (MQ=255)
                                                      cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATAttt                                                                                                 <  1:2308920/150‑1 (MQ=255)
                                                        tcGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAAATTCCGGCAATATTTAt                                                                                               <  2:4056955/149‑1 (MQ=255)
                                                            ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatt                                                                                           >  2:1365002/1‑150 (MQ=255)
                                                               gAAGTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttca                                                                                        <  2:114252/150‑1 (MQ=255)
                                                                aaTTTGCACGACATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAACAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttcat                                                                                       <  2:1857660/150‑1 (MQ=255)
                                                                 aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTAtcatttcatt                                                                                      <  2:3330808/150‑1 (MQ=255)
                                                                    tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaac                                                                                   <  1:3314722/150‑1 (MQ=255)
                                                                    tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTaac                                                                                   <  1:2746345/150‑1 (MQ=255)
                                                                        cGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCTTAAATATTTATCATTTCATTAACAACt                                                                               <  1:3662017/150‑1 (MQ=255)
                                                                         gAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTg                                                                              >  2:3429480/1‑150 (MQ=255)
                                                                           aaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaa                                                                            <  2:2637690/150‑1 (MQ=255)
                                                                            aTCGTAGGGCAGTTAAGGCGTTCACGCCGCATCTGGCATCGAACGCCTTGCCTGACATAAAAGTGCCGTAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaaa                                                                           <  2:867934/150‑1 (MQ=255)
                                                                            aTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGaaa                                                                           <  2:2385396/150‑1 (MQ=255)
                                                                                tAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCtt                                                                       >  2:2815654/1‑150 (MQ=255)
                                                                                    gCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaatt                                                                   >  1:3431728/1‑150 (MQ=255)
                                                                                     cAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaatta                                                                  <  1:4139829/150‑1 (MQ=255)
                                                                                      aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGACTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                 >  2:3218230/1‑150 (MQ=255)
                                                                                      aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                 >  2:3112873/1‑150 (MQ=255)
                                                                                      aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCttaattaa                                                                 >  1:117122/1‑150 (MQ=255)
                                                                                       gATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATAATTATCATTTCATTAACAACTGAAACCTTAATTaaa                                                                <  1:1794181/150‑1 (MQ=255)
                                                                                       gATAAGGCGGTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTaaa                                                                <  2:3631912/150‑1 (MQ=255)
                                                                                         tAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACa                                                              >  1:2449727/1‑150 (MQ=255)
                                                                                         tAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACa                                                              >  2:3693343/1‑150 (MQ=255)
                                                                                           aGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACAtt                                                            >  1:1208739/1‑150 (MQ=255)
                                                                                            ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACCTTa                                                           >  1:392197/1‑150 (MQ=255)
                                                                                            ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                           <  1:985898/150‑1 (MQ=255)
                                                                                            ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                           <  1:1258146/150‑1 (MQ=255)
                                                                                            ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                           >  1:2417661/1‑150 (MQ=255)
                                                                                            ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTa                                                           >  1:1701464/1‑150 (MQ=255)
                                                                                             gCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATGATATATTCCGGCAATATGTAGCATTTCATTAACAACTGAAACCTTATATAAACAGTAg                                                          >  1:4329338/1‑150 (MQ=255)
                                                                                               gTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCATAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGcc                                                        >  2:28874/1‑150 (MQ=255)
                                                                                               gTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCATAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGcc                                                        >  2:2239830/1‑150 (MQ=255)
                                                                                               gTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCCAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACAGTAGcc                                                        >  1:1555689/1‑150 (MQ=255)
                                                                                                ttCACTCCGCATCTTGCAAAGAACGCCTTGCCTGACATAAAATTGCCTGAGAATATCTCCGGCATTTTTATTCCACATCCAAACTCATAATTTATTCCGGAAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCa                                                       <  1:560890/150‑1 (MQ=255)
                                                                                                ttCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCa                                                       >  2:1167669/1‑150 (MQ=255)
                                                                                                ttCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCa                                                       <  1:1755935/150‑1 (MQ=255)
                                                                                                  cACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGt                                                     <  2:2328493/150‑1 (MQ=255)
                                                                                                   aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAGTCATAATATATTCCGGCAATATTTATCATTTCATTATAAAGTGAAACCTTAATTAAACATTAGCCAGTc                                                    >  1:2534739/1‑150 (MQ=255)
                                                                                                   aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTCATTAAACAGTAGCCCGTc                                                    >  1:2282118/1‑150 (MQ=255)
                                                                                                   aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTc                                                    >  2:538993/1‑150 (MQ=255)
                                                                                                   aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTc                                                    >  1:356210/1‑150 (MQ=255)
                                                                                                   aCGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTc                                                    >  1:3997817/1‑150 (MQ=255)
                                                                                                       cgcATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAATTTTATTCCACAGCCAAACTCATAATATATTCCCGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCgtg                                                <  2:2121717/150‑3 (MQ=255)
                                                                                                             tGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTc                                          >  1:2380363/1‑150 (MQ=255)
                                                                                                             tGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTc                                          >  1:1292779/1‑150 (MQ=255)
                                                                                                              ggCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCa                                         <  2:446629/150‑1 (MQ=255)
                                                                                                                 aacgaacgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCCAACGCATAATATATTCCGGCAATGGTTATCATTTCAGTAACAACTGTAACCTTAAGTACACATTAGCCAGGCCGGGTGCTTCACTa                                      >  1:2730345/1‑150 (MQ=255)
                                                                                                                  acgaacgCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTAt                                     <  1:480581/150‑1 (MQ=255)
                                                                                                                    gaacgCCTTGCCTGACATAAATGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTc                                   >  1:1462387/1‑150 (MQ=255)
                                                                                                                        gCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAt                               <  1:2261759/150‑1 (MQ=255)
                                                                                                                        gCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAt                               <  2:1575691/150‑1 (MQ=255)
                                                                                                                          cTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATta                             >  2:759105/1‑150 (MQ=255)
                                                                                                                          cTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATta                             >  1:1089503/1‑150 (MQ=255)
                                                                                                                              ccTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATAtt                         >  1:4206779/1‑150 (MQ=255)
                                                                                                                               cTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACAGTAGCCAGTCCGGGTAATGCACTATTCGAATTATAttt                        >  2:3374616/1‑150 (MQ=255)
                                                                                                                                tGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATAtttt                       >  2:1500114/1‑150 (MQ=255)
                                                                                                                                  gaaTAAAATTGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTCTTCGAATTATATTTTCg                     >  1:2755598/3‑150 (MQ=255)
                                                                                                                                    aTAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTAGTCGAATTATATTTTCGCt                   >  1:83392/1‑150 (MQ=255)
                                                                                                                                     tAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTACACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTg                  <  1:2577457/150‑1 (MQ=255)
                                                                                                                                       aaaGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCg                >  1:3138308/1‑150 (MQ=255)
                                                                                                                                        aaGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGa               <  1:98142/150‑1 (MQ=255)
                                                                                                                                         aatGCCGGAGATATTCTCCGGCACTTTTATTCCACAGCCAATCTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAATCCTTAATTAATCATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGat              >  1:2004542/3‑150 (MQ=255)
                                                                                                                                           tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGatat            >  2:1917710/1‑150 (MQ=255)
                                                                                                                                           tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGatat            >  1:3246507/1‑150 (MQ=255)
                                                                                                                                           tGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAAGTATATTTTCGCTGCGatat            <  1:606807/150‑1 (MQ=255)
                                                                                                                                                  gaATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTg     >  1:3412458/1‑150 (MQ=255)
                                                                                                                                                   aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa    <  2:1135086/150‑1 (MQ=255)
                                                                                                                                                   aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa    <  2:2306184/150‑1 (MQ=255)
                                                                                                                                                   aaTATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAAACTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGa    <  2:4342791/150‑1 (MQ=255)
                                                                                                                                                    atatCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAGTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGAg   <  1:1601833/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGGCAATATTTATCATTTCATTAACAACTGAAACCTTAATTAAACATTAGCCAGTCCGGGTAATTCACTATTCGAATTATATTTTCGCTGCGATATAACCTTGAGC  >  NC_000913/3539667‑3539965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑