Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 287 | Δ1 bp | 5.4% | intergenic (+32/‑50) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 287 | 0 | C | . | 5.4% | 403.2 / 16.6 | 129 | intergenic (+32/‑50) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
Reads supporting (aligned to +/- strand): ref base C (62/58); new base . (2/5); total (66/63) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.73e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 |
Only 100 of 130 total aligned reads displayed.
AATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTG > NC_000913/139‑435 | ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACCCCATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAACCACAGAAAAAAGCCCGCCCCTGACA‑g > 1:1847213/1‑150 (MQ=255) tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCg > 1:1443797/1‑150 (MQ=255) gcgcTCAGACAGATAAAAATTAAAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTAACA‑GTGCggg < 1:1793090/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGc < 2:1219737/150‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCt > 1:704359/1‑150 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCt > 2:2010942/1‑150 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCt > 2:1112913/1‑150 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGCt < 2:662681/150‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGCt < 2:1153160/150‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttt < 2:1087923/150‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttt < 2:20340/150‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGCttt < 1:1677215/150‑1 (MQ=255) cagacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTCCAGGAAACACAGAAAAAAGCCCGCACCTGACC‑GTGCGGGCtttt > 2:1004121/1‑150 (MQ=255) agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCCCAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGCttttt < 1:1548930/150‑1 (MQ=255) acagaTAAAAATTACACAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttttttt < 2:1243508/150‑1 (MQ=255) cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCtttttttt < 2:1615167/150‑1 (MQ=255) cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCtttttttt < 2:590723/150‑1 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttttttttt < 1:662833/150‑1 (MQ=255) taTAAAAATTACAGAGAACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTc < 1:521657/149‑1 (MQ=255) gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTCACGGTGCGGGCTGACGCGTACAGGCAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTc > 1:1461790/1‑150 (MQ=255) gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTc > 1:1409295/1‑150 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCCCCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAACCACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCg < 1:384387/150‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGGTTACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCTCCAGACA‑GTGCGGGCtttttttttag > 2:182607/1‑148 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCg < 1:723221/150‑1 (MQ=255) aaaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGCttttttctgtgtt < 1:1663288/150‑8 (MQ=38) aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGAcc > 1:970617/1‑150 (MQ=255) aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGAcc < 1:1281506/150‑1 (MQ=255) ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACATAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAg < 1:591399/150‑1 (MQ=255) ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGAAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAg < 1:393461/150‑1 (MQ=255) cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACATGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGTCCCAAGGTa < 1:1176116/150‑1 (MQ=255) agTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACg < 2:473257/150‑1 (MQ=255) caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTTCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaa > 2:1718975/1‑150 (MQ=255) caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaa > 1:478478/1‑150 (MQ=255) acaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaaca > 2:602880/1‑150 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa > 1:396385/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg > 1:1909037/1‑150 (MQ=255) aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa > 2:1399930/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:669956/1‑150 (MQ=255) cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAgtg > 1:1946571/1‑150 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGACGTAACAACCATGCGAGTGTTg > 1:381169/1‑150 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa > 1:1936404/1‑150 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:453319/150‑1 (MQ=255) aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTACGACCAAAGGTAACGAGGTAACAACCATCCCAGTGTTGaaa > 1:1867240/1‑149 (MQ=255) gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCCTGCGAGTGTTGAAGt > 2:1288085/1‑150 (MQ=255) gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt > 1:700487/1‑150 (MQ=255) caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt > 2:1751304/1‑150 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCTCCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTGACAACCATGCGAGTGTTGAAGTTcg > 1:368723/1‑150 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTGTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:530428/1‑150 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAAAGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCAACGGACA‑GTGCGGGCTTTTTTTTTCGGCCAAAGGGAACGAGGTAACGACCAAGGGAGTGTTGAAGTTcg > 2:1608458/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCAACTTACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGCGGTGACAACCATGCGAGTGTTGAAGTTcgg > 2:110401/1‑150 (MQ=255) accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc < 2:34485/150‑1 (MQ=255) accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc < 2:396385/150‑1 (MQ=255) ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAAGCGAGTGTTGAAGTTcggcg > 2:851768/1‑150 (MQ=255) accaccaccATCACCATTACCACAGGTAACCGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTCACA‑GTGCGGGCATTTTTTTTCGACCAAAGGTAAAGACGTAACAACCAAGCGAGTGTTGAAGTTCGGCGGTACa > 2:1737317/1‑150 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAGTGTTGAAGTTCGGCGGTACCTCa > 2:568595/1‑150 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa > 2:1922899/1‑150 (MQ=255) ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg > 2:825577/1‑150 (MQ=255) catcaccatTACCCCAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCCCCAGACA‑CTGCGGGCATTTTTTTTCGACCAAAAGGAACGAGGTAAACACCATGCGAGTGTTGAAGTTCGGCGGTTCCTCAGTGGc > 1:1301271/1‑150 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc < 2:1627343/150‑1 (MQ=255) ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg < 1:1733896/150‑1 (MQ=255) catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc < 1:1751305/150‑1 (MQ=255) catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc > 2:653278/1‑150 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa < 1:657518/150‑1 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGAGGGCTTTTTTTATCTACCAAACGTAACGAGGTAACAACCATGCGAGTGATGAAGTTCGGCGGTACATCAGTGTCAAATGCAGaa > 1:322798/1‑150 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTCCATCAGTGGAAAATGCAGCAc > 1:1992638/1‑150 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc < 1:838910/150‑1 (MQ=255) aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 2:761872/1‑150 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGgttt > 2:1472199/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 1:1366488/150‑1 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 1:1370065/150‑1 (MQ=255) aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg < 2:1372547/150‑1 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg < 2:1443796/150‑1 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 2:1389327/150‑1 (MQ=255) gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGtt > 2:555577/1‑150 (MQ=255) cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTg > 2:1830935/1‑150 (MQ=255) gCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGCCA‑GTGAGGGCTTTTTTTTTAAACAAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCTGCGGTACATCAGTGGCAAATGAAGAACGTATTCTGCGTTTTGACg > 1:486249/1‑150 (MQ=255) gCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg < 2:48427/150‑1 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat < 1:2002271/150‑1 (MQ=255) cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGTTAtt > 2:450988/1‑150 (MQ=255) tACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCTAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 2:1266419/150‑1 (MQ=255) tACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGATGAAGTTCGGCGGTACATCAGTGACAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 1:1188956/150‑1 (MQ=255) aCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa > 2:165873/1‑150 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAAGTTTTCTGCGTGTTGCCGATATTCTGGAAAg < 1:1084969/150‑1 (MQ=255) aaaaCACACAAAAAAGCCCGCACCCGACA‑GTGCGGGCTTTTTTTTTCGATCAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc < 2:1036984/149‑1 (MQ=255) gaaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAACAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:1235149/5‑150 (MQ=38) caGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTAGAACAAAGGTAACGACTGAAAAACCAAGCGAGTGTTGAAGTTCTGCGGGACATAAGTGGCAAATGCAGAACGTTTTCACCGTGTTGCCGAGATTCTGGAAAGCAATGcc > 2:1905666/1‑150 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCATGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:1384425/1‑150 (MQ=255) aaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTTAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggc < 2:1461789/150‑1 (MQ=255) aaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTGGCCGATATTCTGGAAAGCAATGCccggc > 2:286391/1‑150 (MQ=255) aaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGAGTTGAAGTTCGGCGGGACATCAGTGGCAAATGCAGAACGTTTTCGGCGGGTTGCCGCTATTCTGAAAACCAATGCcagac > 1:417419/1‑148 (MQ=255) aaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca > 1:1141556/1‑150 (MQ=255) aaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag < 1:1323006/150‑1 (MQ=255) aaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 1:1925185/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 1:1949193/1‑150 (MQ=255) aGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 1:1374619/1‑150 (MQ=255) gCCCGCACCGGACA‑GTGCGGGCTTTTTTTTTATACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGAAGAACTTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg < 2:1818186/150‑1 (MQ=255) gCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGCACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg > 1:1662017/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 1:920365/1‑150 (MQ=255) aCCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg < 2:1231863/150‑1 (MQ=255) aCCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg < 2:1038950/150‑1 (MQ=255) | AATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTG > NC_000913/139‑435 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |