Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 287 Δ1 bp 5.4% intergenic (+32/‑50) thrL → / → thrA thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132870C. 5.4% 403.2 / 16.6 129intergenic (+32/‑50)thrL/thrAthr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (62/58);  new base . (2/5);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 130 total aligned reads displayed.

AATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTG  >  NC_000913/139‑435
                                                                                                                                                    |                                                                                                                                                     
      ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACCCCATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAACCACAGAAAAAAGCCCGCCCCTGACAg                                                                                                                                               >  1:1847213/1‑150 (MQ=255)
          tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCg                                                                                                                                           >  1:1443797/1‑150 (MQ=255)
            gcgcTCAGACAGATAAAAATTAAAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTAACAGTGCggg                                                                                                                                         <  1:1793090/150‑1 (MQ=255)
             cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGc                                                                                                                                        <  2:1219737/150‑1 (MQ=255)
              gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                                                                       >  1:704359/1‑150 (MQ=255)
              gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                                                                       >  2:2010942/1‑150 (MQ=255)
              gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                                                                       >  2:1112913/1‑150 (MQ=255)
              gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCt                                                                                                                                       <  2:662681/150‑1 (MQ=255)
              gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCt                                                                                                                                       <  2:1153160/150‑1 (MQ=255)
                acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttt                                                                                                                                     <  2:1087923/150‑1 (MQ=255)
                acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttt                                                                                                                                     <  2:20340/150‑1 (MQ=255)
                acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttt                                                                                                                                     <  1:1677215/150‑1 (MQ=255)
                 cagacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTCCAGGAAACACAGAAAAAAGCCCGCACCTGACCGTGCGGGCtttt                                                                                                                                    >  2:1004121/1‑150 (MQ=255)
                  agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCCCAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttt                                                                                                                                   <  1:1548930/150‑1 (MQ=255)
                    acagaTAAAAATTACACAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt                                                                                                                                 <  2:1243508/150‑1 (MQ=255)
                     cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                                                                <  2:1615167/150‑1 (MQ=255)
                     cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                                                                <  2:590723/150‑1 (MQ=255)
                      agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttttt                                                                                                                               <  1:662833/150‑1 (MQ=255)
                       taTAAAAATTACAGAGAACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTc                                                                                                                              <  1:521657/149‑1 (MQ=255)
                       gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTCACGGTGCGGGCTGACGCGTACAGGCAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTc                                                                                                                              >  1:1461790/1‑150 (MQ=255)
                       gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTc                                                                                                                              >  1:1409295/1‑150 (MQ=255)
                        aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCCCCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAACCACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCg                                                                                                                             <  1:384387/150‑1 (MQ=255)
                        aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGGTTACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCTCCAGACAGTGCGGGCtttttttttag                                                                                                                             >  2:182607/1‑148 (MQ=255)
                        aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCg                                                                                                                             <  1:723221/150‑1 (MQ=255)
                          aaaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgtgtt                                                                                                                           <  1:1663288/150‑8 (MQ=38)
                           aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAcc                                                                                                                          >  1:970617/1‑150 (MQ=255)
                           aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAcc                                                                                                                          <  1:1281506/150‑1 (MQ=255)
                               ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACATAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAg                                                                                                                      <  1:591399/150‑1 (MQ=255)
                               ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGAAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAg                                                                                                                      <  1:393461/150‑1 (MQ=255)
                                  cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACATGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGTCCCAAGGTa                                                                                                                   <  1:1176116/150‑1 (MQ=255)
                                     agTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACg                                                                                                                <  2:473257/150‑1 (MQ=255)
                                           caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTTCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaa                                                                                                          >  2:1718975/1‑150 (MQ=255)
                                           caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaa                                                                                                          >  1:478478/1‑150 (MQ=255)
                                             acaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaaca                                                                                                        >  2:602880/1‑150 (MQ=255)
                                              caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa                                                                                                       >  1:396385/1‑150 (MQ=255)
                                                   aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg                                                                                                  >  1:1909037/1‑150 (MQ=255)
                                                      aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa                                                                                               >  2:1399930/1‑150 (MQ=255)
                                                       aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg                                                                                              >  2:669956/1‑150 (MQ=255)
                                                         cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAgtg                                                                                            >  1:1946571/1‑150 (MQ=255)
                                                            aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGACGTAACAACCATGCGAGTGTTg                                                                                         >  1:381169/1‑150 (MQ=255)
                                                              tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                                                                       >  1:1936404/1‑150 (MQ=255)
                                                              tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                                                                       <  1:453319/150‑1 (MQ=255)
                                                               aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTACGACCAAAGGTAACGAGGTAACAACCATCCCAGTGTTGaaa                                                                                      >  1:1867240/1‑149 (MQ=255)
                                                                gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCCTGCGAGTGTTGAAGt                                                                                     >  2:1288085/1‑150 (MQ=255)
                                                                gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt                                                                                     >  1:700487/1‑150 (MQ=255)
                                                                 caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt                                                                                    >  2:1751304/1‑150 (MQ=255)
                                                                   ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCTCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTGACAACCATGCGAGTGTTGAAGTTcg                                                                                  >  1:368723/1‑150 (MQ=255)
                                                                   ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTGTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg                                                                                  >  1:530428/1‑150 (MQ=255)
                                                                   ccaccaTTACCACCACCATCACCATTACCACAGGTAAAGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCAACGGACAGTGCGGGCTTTTTTTTTCGGCCAAAGGGAACGAGGTAACGACCAAGGGAGTGTTGAAGTTcg                                                                                  >  2:1608458/1‑150 (MQ=255)
                                                                    caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCAACTTACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGCGGTGACAACCATGCGAGTGTTGAAGTTcgg                                                                                 >  2:110401/1‑150 (MQ=255)
                                                                     accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                                                                <  2:34485/150‑1 (MQ=255)
                                                                     accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                                                                <  2:396385/150‑1 (MQ=255)
                                                                      ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAAGCGAGTGTTGAAGTTcggcg                                                                               >  2:851768/1‑150 (MQ=255)
                                                                           accaccaccATCACCATTACCACAGGTAACCGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTCACAGTGCGGGCATTTTTTTTCGACCAAAGGTAAAGACGTAACAACCAAGCGAGTGTTGAAGTTCGGCGGTACa                                                                          >  2:1737317/1‑150 (MQ=255)
                                                                              accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAGTGTTGAAGTTCGGCGGTACCTCa                                                                       >  2:568595/1‑150 (MQ=255)
                                                                              accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa                                                                       >  2:1922899/1‑150 (MQ=255)
                                                                                  ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg                                                                   >  2:825577/1‑150 (MQ=255)
                                                                                   catcaccatTACCCCAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCCCCAGACACTGCGGGCATTTTTTTTCGACCAAAAGGAACGAGGTAAACACCATGCGAGTGTTGAAGTTCGGCGGTTCCTCAGTGGc                                                                  >  1:1301271/1‑150 (MQ=255)
                                                                                   catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc                                                                  <  2:1627343/150‑1 (MQ=255)
                                                                                        ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg                                                             <  1:1733896/150‑1 (MQ=255)
                                                                                         catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc                                                            <  1:1751305/150‑1 (MQ=255)
                                                                                         catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc                                                            >  2:653278/1‑150 (MQ=255)
                                                                                             aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa                                                        <  1:657518/150‑1 (MQ=255)
                                                                                             aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGAGGGCTTTTTTTATCTACCAAACGTAACGAGGTAACAACCATGCGAGTGATGAAGTTCGGCGGTACATCAGTGTCAAATGCAGaa                                                        >  1:322798/1‑150 (MQ=255)
                                                                                              ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTCCATCAGTGGAAAATGCAGCAc                                                       >  1:1992638/1‑150 (MQ=255)
                                                                                              ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc                                                       <  1:838910/150‑1 (MQ=255)
                                                                                                  aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt                                                   >  2:761872/1‑150 (MQ=255)
                                                                                                   ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGgttt                                                  >  2:1472199/1‑150 (MQ=255)
                                                                                                    gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc                                                 <  1:1366488/150‑1 (MQ=255)
                                                                                                    gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc                                                 <  1:1370065/150‑1 (MQ=255)
                                                                                                      aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg                                               <  2:1372547/150‑1 (MQ=255)
                                                                                                        cGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg                                             <  2:1443796/150‑1 (MQ=255)
                                                                                                         ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt                                            <  2:1389327/150‑1 (MQ=255)
                                                                                                            gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGtt                                         >  2:555577/1‑150 (MQ=255)
                                                                                                             cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTg                                        >  2:1830935/1‑150 (MQ=255)
                                                                                                                gCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGCCAGTGAGGGCTTTTTTTTTAAACAAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCTGCGGTACATCAGTGGCAAATGAAGAACGTATTCTGCGTTTTGACg                                     >  1:486249/1‑150 (MQ=255)
                                                                                                                gCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg                                     <  2:48427/150‑1 (MQ=255)
                                                                                                                    aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat                                 <  1:2002271/150‑1 (MQ=255)
                                                                                                                     cgcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGTTAtt                                >  2:450988/1‑150 (MQ=255)
                                                                                                                         tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCTAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg                            <  2:1266419/150‑1 (MQ=255)
                                                                                                                         tACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGATGAAGTTCGGCGGTACATCAGTGACAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg                            <  1:1188956/150‑1 (MQ=255)
                                                                                                                          aCAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa                           >  2:165873/1‑150 (MQ=255)
                                                                                                                             ggAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAAGTTTTCTGCGTGTTGCCGATATTCTGGAAAg                        <  1:1084969/150‑1 (MQ=255)
                                                                                                                              aaaaCACACAAAAAAGCCCGCACCCGACAGTGCGGGCTTTTTTTTTCGATCAAAGGTAACGAGGTAACAACCATGCGAGTGTGGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc                       <  2:1036984/149‑1 (MQ=255)
                                                                                                                                   gaaaaaaaaaGCCCGCACTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAACAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc                  >  2:1235149/5‑150 (MQ=38)
                                                                                                                                    caGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGAACAAAGGTAACGACTGAAAAACCAAGCGAGTGTTGAAGTTCTGCGGGACATAAGTGGCAAATGCAGAACGTTTTCACCGTGTTGCCGAGATTCTGGAAAGCAATGcc                 >  2:1905666/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCATGTTGCCGATATTCTGGAAAGCAATGCcagg              >  2:1384425/1‑150 (MQ=255)
                                                                                                                                        aaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTTAAGTGCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggc             <  2:1461789/150‑1 (MQ=255)
                                                                                                                                        aaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTGGCCGATATTCTGGAAAGCAATGCccggc             >  2:286391/1‑150 (MQ=255)
                                                                                                                                        aaaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGAGTTGAAGTTCGGCGGGACATCAGTGGCAAATGCAGAACGTTTTCGGCGGGTTGCCGCTATTCTGAAAACCAATGCcagac             >  1:417419/1‑148 (MQ=255)
                                                                                                                                         aaaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca            >  1:1141556/1‑150 (MQ=255)
                                                                                                                                          aaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag           <  1:1323006/150‑1 (MQ=255)
                                                                                                                                          aaaGCCCGCACCTGACAGTGCGGGCTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg          >  1:1925185/1‑150 (MQ=255)
                                                                                                                                           aaGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg          >  1:1949193/1‑150 (MQ=255)
                                                                                                                                            aGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg         >  1:1374619/1‑150 (MQ=255)
                                                                                                                                             gCCCGCACCGGACAGTGCGGGCTTTTTTTTTATACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGAAGAACTTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg        <  2:1818186/150‑1 (MQ=255)
                                                                                                                                             gCCCGCACTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGCACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg        >  1:1662017/1‑150 (MQ=255)
                                                                                                                                               ccGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca      >  1:920365/1‑150 (MQ=255)
                                                                                                                                                   aCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg  <  2:1231863/150‑1 (MQ=255)
                                                                                                                                                   aCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg  <  2:1038950/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTG  >  NC_000913/139‑435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: