Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,345 T→C 6.2% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,3450TC 6.2% 257.4 / 3.8 97pseudogene (954/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (49/40);  new base C (3/3);  total (54/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGG  >  NC_000913/1466197‑1466494
                                                                                                                                                    |                                                                                                                                                       
ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAATGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                                        <  1:2039725/150‑1 (MQ=255)
 cGGTGACAGCTCGACGTTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg                                                                                                                                                       <  2:1837340/150‑1 (MQ=255)
  ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAACCTCAATAACGAAGGCGAGAGCACCATCACCACCGCTGGCACCGGCACCCAGATTAATGGt                                                                                                                                                      >  2:874593/1‑150 (MQ=255)
   gTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtg                                                                                                                                                     <  1:1626815/150‑1 (MQ=255)
   gTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGACAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtg                                                                                                                                                     <  2:2040078/150‑1 (MQ=255)
     gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgat                                                                                                                                                  >  2:1146517/1‑150 (MQ=255)
       cAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga                                                                                                                                                >  2:695706/1‑150 (MQ=255)
       cAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCAATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGAACCAGCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga                                                                                                                                                >  2:87488/1‑150 (MQ=255)
        aGCGCGACGGTGGATAACAAGGTCACCATGACCGTCACCGATCAGGATTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGGTGAc                                                                                                                                               <  1:329877/150‑1 (MQ=255)
        aGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGAc                                                                                                                                               <  2:73362/150‑1 (MQ=255)
         gcgcGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACg                                                                                                                                              >  2:772461/1‑150 (MQ=255)
           gcgACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGcc                                                                                                                                            >  1:1748073/1‑150 (MQ=255)
                     aTAACAAGGGCACCATGACCGTCACCGGTCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                                 <  1:1634790/150‑1 (MQ=255)
                     aTAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAGGGGTATCCAGAGCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                                 <  1:1901554/150‑1 (MQ=255)
                       aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGCCGCCACGGCAaacaac                                                                                                                               >  1:1757154/1‑150 (MQ=255)
                       aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaac                                                                                                                               >  2:570116/1‑150 (MQ=255)
                            gggCACCATGACCGTCACCGATCCGGAGTCCATGCGTATCCAGATCGACGGTGACAAGGCCATCGGCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                          <  1:335649/150‑1 (MQ=255)
                              gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCa                                                                                                                        <  1:984178/150‑1 (MQ=255)
                              gCACCATGACCGTCACCGATCCGGAGTCCATGGTTTACACATATAAGGTGACAAGGCCATATTAAATAAAGAAGGCGACCGCACCATCCCCAAAGGTTGCCCCCGCACCCATATTAATGGTGATTCACCCCACGCAAACAAACAACgcaa                                                                                                                        >  2:1972634/1‑147 (MQ=12)
                                 ccATGGCCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGACAGCACCATCACCAACTGTGGCACCGTCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCaaaa                                                                                                                     <  1:321140/150‑1 (MQ=255)
                                    tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccc                                                                                                                  >  1:1976136/1‑149 (MQ=255)
                                    tGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAacca                                                                                                                  >  2:577961/1‑150 (MQ=255)
                                       ccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg                                                                                                               <  1:1624479/150‑1 (MQ=255)
                                          tCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTg                                                                                                            >  2:1347632/1‑150 (MQ=255)
                                           cACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCCAACAACAACGGCAAAACCACCGTTGa                                                                                                           >  1:861112/1‑150 (MQ=255)
                                            aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTgcc                                                                                                          >  1:536533/1‑148 (MQ=255)
                                             ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                                         <  2:1817879/150‑1 (MQ=255)
                                             ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                                         >  1:1453077/1‑150 (MQ=255)
                                              cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                                        >  2:237139/1‑150 (MQ=255)
                                               gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                                       >  1:898920/1‑150 (MQ=255)
                                               gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                                       <  2:91759/150‑1 (MQ=255)
                                                 tCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                                     >  2:101963/1‑150 (MQ=255)
                                                  ccGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGCTGGCCCCGGCACCCAGCTTAATGGTGATGACGCCCCGGCCAACAACAACGGCAAAACCACCGATGACGGCAAg                                                                                                    >  2:302237/1‑150 (MQ=18)
                                                   cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                                   <  2:1161641/150‑1 (MQ=255)
                                                      aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                                >  1:619973/1‑150 (MQ=255)
                                                         ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa                                                                                             >  2:1673018/1‑150 (MQ=255)
                                                          cATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                            >  1:774707/1‑150 (MQ=255)
                                                           aTGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                           <  2:1614117/150‑1 (MQ=255)
                                                              ggTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGt                                                                                        >  2:1598683/1‑150 (MQ=255)
                                                               gTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGTCAAAACCACCGTTGACGGCAAGGATTCCACCggga                                                                                       >  1:978736/1‑148 (MQ=255)
                                                               gTATCAAGATCGACGGTGACAATGCAATCGTAAATAACGAAGGAGATATCACCTTCTCAAAGGGTTGCTCCGTCACCCAGATTAATGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                                       <  2:1561018/150‑1 (MQ=17)
                                                                  tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAATGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                                    >  2:1080651/1‑150 (MQ=255)
                                                                  tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                                    >  1:1887426/1‑150 (MQ=255)
                                                                    cAGATCGACGGTGACAAGGCCACCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                                  >  1:659081/1‑150 (MQ=255)
                                                                     aGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaaa                                                                                 <  2:18750/150‑1 (MQ=255)
                                                                      gATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAt                                                                                >  2:1993180/1‑150 (MQ=255)
                                                                       aTCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATc                                                                               >  2:1633822/1‑150 (MQ=255)
                                                                        tCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAttg                                                                              <  1:1598684/150‑3 (MQ=255)
                                                                         cGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGTTTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCaa                                                                             >  2:1989893/1‑150 (MQ=255)
                                                                          gACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACg                                                                                                                                         <  1:775052/90‑1 (MQ=14)
                                                                          gACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACg                                                                                                                                         >  2:775051/1‑90 (MQ=17)
                                                                           aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                           <  2:906772/150‑1 (MQ=255)
                                                                           aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                           <  1:1146518/150‑1 (MQ=255)
                                                                           aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACAAACGGTGGCACCGGAACACAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGATGACGGCAAGGATTCCACCTGTACGGAAATaaaag                                                                           >  1:1112339/1‑148 (MQ=17)
                                                                            cGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACgg                                                                          >  1:853788/1‑150 (MQ=255)
                                                                             ggTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGt                                                                         <  2:1453076/150‑1 (MQ=255)
                                                                               tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaa                                                                       >  1:21751/1‑150 (MQ=255)
                                                                                 aCAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCATATTAATGGTGATGACGCCACGGCAAACAACAACTGCAAAACCACCGTTGACGGCAAGGATTCCACCGTTACGGAAATCAACGGtaata                                                                     <  1:1250598/150‑1 (MQ=255)
                                                                                   aaGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAAc                                                                   <  2:1809167/150‑1 (MQ=255)
                                                                                       ccATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                          >  2:2004496/1‑91 (MQ=255)
                                                                                       ccATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                          <  1:2004497/91‑1 (MQ=255)
                                                                                           cGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAc                                                                                                                                               >  2:731100/1‑67 (MQ=255)
                                                                                                aTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTc                                                      >  2:1331559/1‑150 (MQ=255)
                                                                                                  aaCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAg                                                    <  1:1382089/150‑1 (MQ=255)
                                                                                                   aCGAAGGCGAGAGCACCATCACAAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACAACCGTTGACGGCAAGGAATCCACCGGTACGGAAATAAAAGGAAATAACGGAAAAGTTATTCAgg                                                   >  1:55651/1‑150 (MQ=14)
                                                                                                        ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc                                              <  1:695707/150‑1 (MQ=255)
                                                                                                          cGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGCCGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATTACGGAAAAGTTATTCAGGACGGCGa                                            >  2:1074014/1‑150 (MQ=255)
                                                                                                            agagCACCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                                         >  2:1237733/1‑87 (MQ=18)
                                                                                                            agagCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCTCCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATc                                          <  2:898919/150‑1 (MQ=255)
                                                                                                                cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                                      <  2:1243004/150‑1 (MQ=255)
                                                                                                                  ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGt                                                                                                              >  2:1311270/1‑76 (MQ=39)
                                                                                                                  ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGt                                                                                                              <  1:1311271/76‑1 (MQ=39)
                                                                                                                  ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAGCTGGATg                                    >  1:1275500/1‑150 (MQ=255)
                                                                                                                     tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCa                                 >  2:496950/1‑150 (MQ=255)
                                                                                                                      cACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAg                                >  2:1571477/1‑150 (MQ=255)
                                                                                                                       aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                                    >  1:1755804/1‑81 (MQ=38)
                                                                                                                           aCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAACCCACCGTTGACGGCAAGGAATCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcg                           <  2:1976135/150‑1 (MQ=255)
                                                                                                                                ggcaccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                      <  1:570116/150‑1 (MQ=255)
                                                                                                                                  caccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAAACGGAAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa                    >  2:676697/1‑150 (MQ=255)
                                                                                                                                   accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                           >  2:1672818/1‑78 (MQ=39)
                                                                                                                                   accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                           <  1:1672819/78‑1 (MQ=39)
                                                                                                                                   accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                   <  1:772462/150‑1 (MQ=255)
                                                                                                                                    ccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAATGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                  <  1:1080652/150‑1 (MQ=255)
                                                                                                                                     cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                                         >  1:1121944/1‑46 (MQ=255)
                                                                                                                                     cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                                         <  2:1121943/46‑1 (MQ=255)
                                                                                                                                     cggcaccCAGATAAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGGACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                 <  1:101963/150‑1 (MQ=255)
                                                                                                                                       gcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                                    >  2:807604/1‑65 (MQ=40)
                                                                                                                                       gcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                                    <  1:807605/65‑1 (MQ=40)
                                                                                                                                          cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                                >  1:795273/1‑66 (MQ=39)
                                                                                                                                          cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                                <  2:795272/66‑1 (MQ=39)
                                                                                                                                          cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGAGGCGGTCACGGTATTg            >  2:1374031/1‑150 (MQ=255)
                                                                                                                                            cAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGaa          >  1:1148697/1‑149 (MQ=255)
                                                                                                                                             aGATTAATGGTGATGACGCCACGGCAAACAACAACTGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAAAGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata         <  1:87488/150‑1 (MQ=255)
                                                                                                                                              gATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATATCGGAAAAGTTATTCAGGACGGAGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGatat        <  1:302237/150‑1 (MQ=255)
                                                                                                                                                 tAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg                                                                                     <  2:361731/70‑1 (MQ=35)
                                                                                                                                                 tAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg                                                                                     >  1:361731/1‑70 (MQ=35)
                                                                                                                                                  aaTGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCTACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAcc    >  1:1707080/1‑150 (MQ=255)
                                                                                                                                                    tGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGCCGGCGATCTGGATGTCAGCGGCGGCGGTCACGGGATTGATATCAcctg  >  1:404729/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
CCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGG  >  NC_000913/1466197‑1466494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: