Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,782 C→T 5.3% intergenic (‑113/+44) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7820CT 5.3% 361.1 / 5.3 117intergenic (‑113/+44)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (52/56);  new base T (3/3);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 121 total aligned reads displayed.

TATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCA  >  NC_000913/1555633‑1555931
                                                                                                                                                     |                                                                                                                                                     
tattAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGTGGGGCTTTTATCGTCTTTGCTTTACCGCCAGTGCGTCTGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcgc                                                                                                                                                       <  1:617236/150‑1 (MQ=255)
  taAAGCGAACGCTGTTTTTTTGTTTTTTGTTCCATTTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGGTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCTCAg                                                                                                                                                     <  1:919760/150‑1 (MQ=255)
   aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                    <  1:1014617/150‑1 (MQ=255)
   aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                    >  2:751092/1‑150 (MQ=255)
   aaaGCGAACACTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                    <  2:1167939/150‑1 (MQ=255)
    aaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATATCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGc                                                                                                                                                   >  2:946025/1‑150 (MQ=255)
       cGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAAt                                                                                                                                                >  1:848265/1‑150 (MQ=255)
           ctcTGTTTTTTTTTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATACCTTGGTTGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGTCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                            <  1:1286670/148‑1 (MQ=255)
           cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                            <  1:702471/150‑1 (MQ=255)
            gCTGTTTTTTTGTTTTTGGTTACATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTAATATCGTCTTTGCAGTACCGCCAGGGCGTCGTCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtgt                                                                                                                                           <  1:1823127/150‑1 (MQ=255)
              ggTTTTTTTGTTTTTTGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGttt                                                                                                                                         <  2:943543/149‑1 (MQ=255)
               gTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTa                                                                                                                                        >  1:1895439/1‑150 (MQ=255)
                 ttttttGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAAt                                                                                                                                      <  2:1015404/150‑1 (MQ=255)
                  tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCTTCTTTGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAATAACACCATTGCGAAGGCAATGGTGTTTAATc                                                                                                                                     >  1:1220952/1‑150 (MQ=255)
                  tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATc                                                                                                                                     >  1:754799/1‑150 (MQ=255)
                  tttttgtttatgGTACCATGTCACTCACTCTTTATTGAATCTCCATCCCTTCGCGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATc                                                                                                                                     <  1:273630/150‑1 (MQ=255)
                   ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                    <  1:1442069/150‑1 (MQ=255)
                   ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                    <  2:1465592/150‑1 (MQ=255)
                   ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgtt                                                                                                                                    <  2:689757/150‑6 (MQ=255)
                    tttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCATCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTTTAAAGCAGATAAAAACAAAAACACCATTGCGCCGGCATTGGTGTTTAATCGt                                                                                                                                   >  2:416888/1‑150 (MQ=255)
                     ttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCGTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAATGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTc                                                                                                                                  <  2:1576333/150‑1 (MQ=255)
                         ttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTg                                                                                                                              <  2:651165/150‑1 (MQ=255)
                          tttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGa                                                                                                                             >  2:1702628/1‑150 (MQ=255)
                          tttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGACTTTGCTTTACCGCCAGGGCGTCGGCCGCAAGTATACAGCAGATAAAAACAAAAACACCATTGAGCAGGCAATGGTGTTTAAACTTCATTGa                                                                                                                             >  2:1340039/1‑150 (MQ=255)
                            tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                           >  1:971995/1‑150 (MQ=255)
                               ttCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACt                                                                                                                        <  2:1540950/150‑1 (MQ=255)
                                 ccATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGa                                                                                                                      <  1:934495/150‑1 (MQ=255)
                                 ccATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGa                                                                                                                      >  2:1654028/1‑150 (MQ=255)
                                  cATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTgtt                                                                                                                     <  2:268092/150‑3 (MQ=255)
                                  cATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAATGAt                                                                                                                     >  1:613651/1‑150 (MQ=255)
                                      gaactcactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGACAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGtg                                                                                                                 <  2:1230217/148‑2 (MQ=255)
                                        actcactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTAt                                                                                                               <  2:1049393/150‑1 (MQ=255)
                                           cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGACAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGaa                                                                                                            >  2:70134/1‑150 (MQ=255)
                                            actaTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                           <  1:1420408/150‑1 (MQ=255)
                                             ctctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAtt                                                                                                          >  2:255707/1‑150 (MQ=255)
                                             ctctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAtt                                                                                                          <  1:592113/150‑1 (MQ=255)
                                             ctctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAtt                                                                                                          >  1:1271148/1‑150 (MQ=255)
                                                    ttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTc                                                                                                   <  1:1763369/150‑1 (MQ=255)
                                                     tGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCa                                                                                                  >  2:458501/1‑150 (MQ=255)
                                                     tGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTAGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGATGACTGATGGTTATGAATTACTTTTCa                                                                                                  >  1:79922/1‑150 (MQ=255)
                                                       aaTATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGc                                                                                                >  1:1701804/1‑150 (MQ=255)
                                                          atccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCggg                                                                                             >  1:974757/1‑150 (MQ=255)
                                                             catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCTGCGGGGCg                                                                                          >  1:401954/1‑150 (MQ=255)
                                                             catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCg                                                                                          >  1:111824/1‑150 (MQ=255)
                                                              atccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGt                                                                                         >  1:278865/1‑150 (MQ=255)
                                                                   tGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                    <  2:454700/150‑1 (MQ=255)
                                                                        ggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCACAAGTATAAATAAGATAAAAAAAAAAACACCATTGAGCAGGCAATGTTGTTTAATCGTCATTGAGGACTGAGGGTTATGAATTACTTTTCATCGGGGCGATGTCTGCCgg                                                                               >  1:81383/1‑150 (MQ=255)
                                                                           ttttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTg                                                                            <  2:1097582/150‑1 (MQ=255)
                                                                             ttATCGTCTTTGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTTCTCAGGCAATGGTGTTTAATCGTCATTGTGGAATGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGACGGTTggg                                                                          <  2:1345624/150‑1 (MQ=255)
                                                                              tATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                         >  2:831921/1‑150 (MQ=255)
                                                                              tATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                         >  1:1749622/1‑150 (MQ=255)
                                                                               aTCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGtt                                                                        >  2:1624667/1‑150 (MQ=255)
                                                                                tCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTa                                                                       <  2:909659/150‑1 (MQ=255)
                                                                                  gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                     <  1:712945/150‑1 (MQ=255)
                                                                                  gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                     <  1:1413559/150‑1 (MQ=255)
                                                                                    cTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTATTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTta                                                                   >  1:871731/1‑150 (MQ=255)
                                                                                     tttGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtac                                                                  <  1:1806535/150‑1 (MQ=255)
                                                                                         cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAATAACAAAAACACAATTGCGCAGGCAATGGTGTTTAATCGGCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGGGTGTTCTGCCGGTTGGGTAATTTACTACg                                                              >  1:54285/1‑150 (MQ=255)
                                                                                            tACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTg                                                           >  2:1749953/1‑150 (MQ=255)
                                                                                              ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGa                                                         >  1:1078493/1‑150 (MQ=255)
                                                                                                 ccAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAttt                                                      >  1:860328/1‑150 (MQ=255)
                                                                                                    cggCGTCTGCCTCAAGTATAACGCAGAAAAAACCAAAACCACCCTTGCGCAGGCAATGGTGTTTAAACGGCATAGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGAGTTTTCTGCCGGTAGGGGTATTTACTACGCTGGATTTGTc                                                   <  2:1927763/149‑1 (MQ=255)
                                                                                                      gCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCAc                                                 <  1:1975596/150‑1 (MQ=255)
                                                                                                      gCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCAc                                                 <  1:1350729/150‑1 (MQ=255)
                                                                                                         acGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAATGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCtt                                              >  1:679698/2‑150 (MQ=255)
                                                                                                           ggCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCTTTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCg                                            <  2:95060/150‑1 (MQ=255)
                                                                                                           ggCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCg                                            <  1:780487/150‑1 (MQ=255)
                                                                                                              cTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTa                                         <  2:111824/150‑1 (MQ=255)
                                                                                                              cTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTa                                         <  1:873456/150‑1 (MQ=255)
                                                                                                              cTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTa                                         <  2:591292/150‑1 (MQ=255)
                                                                                                                  aGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTa                                     >  2:1829162/1‑150 (MQ=255)
                                                                                                                       aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                                <  2:971994/150‑1 (MQ=255)
                                                                                                                       aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                                >  2:824536/1‑150 (MQ=255)
                                                                                                                       aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                                <  2:1722151/150‑1 (MQ=255)
                                                                                                                          gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                             <  1:1624409/150‑1 (MQ=255)
                                                                                                                          gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                             >  1:956042/1‑150 (MQ=255)
                                                                                                                          gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTACAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                             <  2:1582299/150‑1 (MQ=255)
                                                                                                                            aGATAAAAACAAAAACACCATTGCGCTGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTATTCAGCGGGGCGTGTTCTGCGGGTTCGGTTAGTAACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCtt                           >  1:1341555/1‑149 (MQ=255)
                                                                                                                             gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTgg                          >  2:1510187/1‑150 (MQ=255)
                                                                                                                             gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTgg                          <  1:765385/150‑1 (MQ=255)
                                                                                                                             gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTgg                          >  1:1905305/1‑150 (MQ=255)
                                                                                                                               tAAAAACAAAAACACCATTGCGGAGGCAATGGTGTTTAATCGTCATTGAGGACTAATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGtt                        <  1:1070240/150‑1 (MQ=255)
                                                                                                                                aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                       >  2:2050572/1‑150 (MQ=255)
                                                                                                                                aaaaacaaaaacACCATTGCGCAGGAAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                       >  1:1835953/1‑150 (MQ=255)
                                                                                                                                  aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                     >  2:1190907/1‑150 (MQ=255)
                                                                                                                                   cgattaaacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaa                    >  1:1970677/6‑150 (MQ=255)
                                                                                                                                    acataaaccCCATTGCGCAGGCAATTGTGTATAATCGTCATTGAGGACTGATGGTTATGAATTAGTTTTCAGCGGGGCTTTTTCTGCGGGTTGGGTGATTTACTACGCTTGATTTGTATCCTTGGGTATCTATGTTGCGCTTCTTAGtaa                   >  1:100292/1‑150 (MQ=255)
                                                                                                                                    acaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTGTTCTGCCGGTTGGGTTATTTACTACGCTGGAGTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaaa                   <  2:1855111/150‑1 (MQ=255)
                                                                                                                                     caaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAt                  >  2:846766/1‑150 (MQ=255)
                                                                                                                                      aaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCAGAGCGGACTGAGGGATATGAATTACTTTTCAGCGGGGCGTTGTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAGGATATTGCGCTGGTTAGAAAtt                 >  1:1286498/1‑150 (MQ=255)
                                                                                                                                       aaaacaCCATTGCGCATTCAATGGTGTTTAATCGTCATTTTTTACTGTTGGTTATGTATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAttt                <  1:652581/150‑1 (MQ=255)
                                                                                                                                       aaaacaCCATTGAGCAGGCAATGGTGTTTAATCGTCATTGAGGACTTATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAttt                <  1:660934/150‑1 (MQ=255)
                                                                                                                                        aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt               >  1:1185221/1‑150 (MQ=255)
                                                                                                                                         aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa              >  2:1823414/1‑150 (MQ=255)
                                                                                                                                           cacCATTGCCTGCGCAATGGTGTTAAAACGACATTGAGGACTGATTGTTATGAATTACTTATCAGCGGGGCGTATTCTGCCGGTTGGGTTATTTACTAAAATGGATTTGTCACCTTCGGTAAATATTTTCAACAGGTTAGAAATTTTAtt            >  2:499738/1‑149 (MQ=255)
                                                                                                                                            acCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATgg           <  2:848264/150‑1 (MQ=255)
                                                                                                                                             ccATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgt          <  1:618513/150‑1 (MQ=255)
                                                                                                                                                ttGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcca       <  2:278865/150‑1 (MQ=255)
                                                                                                                                                  gcgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagt     >  2:479435/1‑150 (MQ=255)
                                                                                                                                                     cAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAATTTTTATGgtccagtcca  <  2:1942733/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCA  >  NC_000913/1555633‑1555931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: