Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,620,224 C→G 7.8% intergenic (+17/+14) marB → / ← eamA periplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,620,2240CG 7.8% 406.8 / 22.4 128intergenic (+17/+14)marB/eamAperiplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base C (37/81);  new base G (6/4);  total (43/85)
Fisher's exact test for biased strand distribution p-value = 8.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

Only 100 of 130 total aligned reads displayed.

TACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAG  >  NC_000913/1620075‑1620372
                                                                                                                                                     |                                                                                                                                                     
tACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAc                                                                                                                                                       >  2:1129355/1‑150 (MQ=255)
tACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAc                                                                                                                                                       >  1:1589657/1‑150 (MQ=255)
 aCTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTTAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACa                                                                                                                                                      >  2:24521/1‑150 (MQ=255)
   ttcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGGTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtc                                                                                                                                                    <  2:856034/150‑1 (MQ=255)
       tgttCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCgg                                                                                                                                                <  1:390983/150‑1 (MQ=255)
        gtgCCAATGTCGTGGTTGTTCCCCCATCGCAGGCACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGCTGCGCTCGGCGTGCCCGATTATAATAAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCggg                                                                                                                                               >  2:1801443/1‑150 (MQ=255)
         tgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCgggg                                                                                                                                              <  1:1977926/150‑1 (MQ=255)
          gCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGc                                                                                                                                             <  2:837664/150‑1 (MQ=255)
            cAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCtt                                                                                                                                           <  2:280016/150‑1 (MQ=255)
              aTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGGTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccaga                                                                                                                                         <  2:1515005/150‑5 (MQ=255)
               tGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccagaa                                                                                                                                        <  2:469174/150‑6 (MQ=255)
               tGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                                                        <  2:237988/150‑1 (MQ=255)
                      gttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                                                                 >  1:1736552/1‑150 (MQ=255)
                       ttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                                                                <  1:1409932/150‑1 (MQ=255)
                       ttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATAAACTTccc                                                                                                                                >  1:1652657/1‑150 (MQ=255)
                        tgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcccc                                                                                                                               >  1:1598812/1‑149 (MQ=255)
                        tgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCa                                                                                                                               <  1:837990/150‑1 (MQ=255)
                         gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGGGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAc                                                                                                                              <  2:695698/150‑1 (MQ=255)
                             ccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCttt                                                                                                                          <  1:23008/150‑1 (MQ=255)
                             ccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCttt                                                                                                                          <  2:1414186/150‑1 (MQ=255)
                               ccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                                                        <  1:863989/150‑1 (MQ=255)
                                      aGGAACACCCACCGTTTGATTTTAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTa                                                                                                                 <  1:24521/150‑1 (MQ=255)
                                      aGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTa                                                                                                                 <  2:893807/150‑1 (MQ=255)
                                        gAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                                                               <  2:520726/150‑1 (MQ=255)
                                         aaCACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                                                              <  2:1181598/150‑1 (MQ=255)
                                          acacCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGcc                                                                                                             <  2:1237635/150‑1 (MQ=255)
                                          acacCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTAGGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGcc                                                                                                             <  1:1347009/150‑1 (MQ=255)
                                            acccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCTGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGCCATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAc                                                                                                           >  2:1714219/1‑150 (MQ=255)
                                             cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAcc                                                                                                          <  2:1992896/150‑1 (MQ=255)
                                             cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTACCCACCTTTACAGCTTTACGCCAcc                                                                                                          >  1:554666/1‑150 (MQ=255)
                                              ccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCg                                                                                                         <  2:189406/150‑1 (MQ=255)
                                              ccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCg                                                                                                         >  1:520733/1‑150 (MQ=255)
                                               caccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCCCCTTTACCGCTTTACGCCCCCGc                                                                                                        >  2:1803017/1‑150 (MQ=255)
                                               caccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTAAAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACCTCCCACCTTTACCGCTTTACGCCACCGc                                                                                                        <  2:266389/150‑1 (MQ=255)
                                                  cGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAg                                                                                                     <  2:1908992/150‑1 (MQ=255)
                                                  cGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAg                                                                                                     <  2:866071/150‑1 (MQ=255)
                                                  cGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTGGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAg                                                                                                     <  1:131593/150‑1 (MQ=255)
                                                   gTTTGATTTAAATCACATGTGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGc                                                                                                    <  2:780144/150‑1 (MQ=255)
                                                   gTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGc                                                                                                    >  1:280762/1‑150 (MQ=255)
                                                    tttGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTTTTATAATCAACACGCTATGTAGATTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                                                                   <  2:258847/150‑1 (MQ=255)
                                                        aTTTAAATCACATGGGAACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAACGATTTACGCCACCGCAAGCCAAAt                                                                                               >  1:417656/1‑150 (MQ=255)
                                                         tttAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                                                              >  1:1471559/1‑150 (MQ=255)
                                                             aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCAACCGCAAGCCAAATACAtt                                                                                          >  1:1943951/1‑150 (MQ=255)
                                                              aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                         <  2:1486162/150‑1 (MQ=255)
                                                              aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                         <  2:138108/150‑1 (MQ=255)
                                                              aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                         <  2:200740/150‑1 (MQ=255)
                                                               tCACATGGGTACTGTCATTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGa                                                                                        <  2:1019932/150‑1 (MQ=255)
                                                               tCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGa                                                                                        <  2:59791/150‑1 (MQ=255)
                                                                 acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                                                                      >  2:973191/1‑150 (MQ=255)
                                                                 acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                                                                      <  2:365620/150‑1 (MQ=255)
                                                                 acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                                                                      <  1:1134004/150‑1 (MQ=255)
                                                                        tACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                                                               <  1:1093609/150‑1 (MQ=255)
                                                                           tGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCgg                                                                            <  2:602749/150‑1 (MQ=255)
                                                                            ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAGTGATATCCAGCCCGGt                                                                           >  2:813868/1‑150 (MQ=255)
                                                                             gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                                                          <  1:925265/150‑1 (MQ=255)
                                                                                   aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                    <  1:533466/150‑1 (MQ=255)
                                                                                   aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                    <  1:1371989/150‑1 (MQ=255)
                                                                                   aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                    <  2:1133294/150‑1 (MQ=255)
                                                                                   aTAAGGCGGATGCGCTCGGCGTGACCTATTATAATCAACACGCTATGTAGTTTGTTCTGGACCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                    <  1:240982/150‑1 (MQ=255)
                                                                                    tAAGTCGGATGCGCTCTGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGa                                                                   <  2:418325/150‑1 (MQ=255)
                                                                                      aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgc                                                                 <  2:47304/150‑1 (MQ=255)
                                                                                         cGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTACCTTCCCCCCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGCgcccc                                                              >  2:26256/1‑150 (MQ=255)
                                                                                            aTGCGCGCGGCGTGCCCTATAATCATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTCCGCCACCGCAAGCCAAATACAATGATATACAGCCCGGTCATAATGAgcaccgca                                                           <  1:631234/150‑1 (MQ=255)
                                                                                              gcgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcacc                                                         <  2:1449485/150‑1 (MQ=255)
                                                                                               cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCt                                                        <  2:399180/150‑1 (MQ=255)
                                                                                                gcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTa                                                      >  2:467097/1‑150 (MQ=255)
                                                                                                gcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTa                                                      <  2:525211/150‑1 (MQ=255)
                                                                                                 cTCGGCGTGCCCTATTATAATCAACACGCAATGTAGGTTGTTCTGGCCCCGACAACTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCCCCGCAAGCCAAATACATTCATATACAGCCCGGTCATAATGAGCACCGCCCCGaa                                                     >  1:1697650/1‑150 (MQ=255)
                                                                                                      cGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTTATAATGAGCACCGCACCTAAAAAtt                                                <  2:1652656/150‑1 (MQ=255)
                                                                                                        tGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGc                                              <  1:1409052/150‑1 (MQ=255)
                                                                                                         gCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCGTATTAACTTCCAACCATTACCGCTTTACGCCACCGCAAGCCAACTACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa                                             <  2:90677/150‑1 (MQ=255)
                                                                                                         gCCCCATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa                                             <  2:447781/150‑1 (MQ=255)
                                                                                                          cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACTTCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCTCAAGCCAAATACATTGATATACAGCCAGGTCATAATGAGCACCGCACCTAAAAATTGCAg                                            >  2:1382951/1‑150 (MQ=255)
                                                                                                          cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCTAAATACATTGATATACAGCCCGGTCATAATGAGCCCCGCTCCTAAAAATTGCAg                                            >  2:1310584/1‑150 (MQ=255)
                                                                                                          cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg                                            >  2:409302/1‑150 (MQ=255)
                                                                                                          cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGATTATTAACTTCCCACCTTTACCGCTTTACGCCACCTCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg                                            <  1:1041670/150‑1 (MQ=255)
                                                                                                            cTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCCCCTAAAAATTGCAGAc                                          >  1:1385196/1‑150 (MQ=255)
                                                                                                                tataATCAACACGCTATGTAGTTTGTGCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAAATTGCAGACCCg                                       <  2:1294015/150‑1 (MQ=255)
                                                                                                                    aTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAg                                  >  2:1956996/1‑150 (MQ=255)
                                                                                                                       aaCACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGt                               <  1:1984227/150‑1 (MQ=255)
                                                                                                                        acacTCTTTGTATTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGACACCGCAATCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGtt                              <  2:1438888/150‑1 (MQ=255)
                                                                                                                              tATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAAATTCCCACCTTTACAGCTTTACGCCACCGCAAACCAAATACATTTATATACAGCCCGGTCATAATGAGCACAGAACCTAAAAATTGCAGACCCGTTAAGCGTTCATCca                        >  1:980834/1‑150 (MQ=255)
                                                                                                                              tATGTAGTTTGTGCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCca                        <  2:1803264/150‑1 (MQ=255)
                                                                                                                               aTGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCTTTCATCcaa                       <  2:554666/150‑1 (MQ=255)
                                                                                                                                 ttAGTTTGTTCTGGCCCCGACATCTCGTGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCTCCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaaca                     <  1:1205223/149‑1 (MQ=255)
                                                                                                                                        taataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATATTGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTGATCCATCAATACTGc              >  1:195881/7‑150 (MQ=255)
                                                                                                                                        taataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCAGCCAACAATAGTGc              >  1:1024375/7‑150 (MQ=255)
                                                                                                                                        taataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGGCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTACGCGTTCATCCAACAATAGTGc              >  1:1031222/7‑150 (MQ=255)
                                                                                                                                         ttCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGcc             <  1:1578345/150‑1 (MQ=255)
                                                                                                                                         aataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGcc             >  2:1152764/6‑150 (MQ=255)
                                                                                                                                         aataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGGCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAAAAATAGTGcc             >  2:1682483/6‑150 (MQ=255)
                                                                                                                                         aataagCCCCGAGATGTCGCGGCTTATTAACTAACCACCTTTACCGCTTTACGCCACCGCAACCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCATACCCGTTAAGAGTTCATCCAACAAAAGTGcc             >  1:73947/6‑150 (MQ=255)
                                                                                                                                          tCTGGCCCCGACAACTCGGGGCTTTTTAAATTACCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATTCAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCg            <  2:1031588/150‑1 (MQ=255)
                                                                                                                                             ggCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCCCCTAAAAATTGCAGCCCCGTTAAGCGTTCATCCAACAATAGTGCCGCAc         >  2:1819493/1‑150 (MQ=255)
                                                                                                                                             ggCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCAc         >  2:809283/1‑150 (MQ=255)
                                                                                                                                              gCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACt        <  2:1746308/150‑1 (MQ=255)
                                                                                                                                                cccGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTg      <  1:1152765/150‑1 (MQ=255)
                                                                                                                                                cccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTg      <  2:1360555/150‑1 (MQ=255)
                                                                                                                                                    aCATCTCGGTGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAg  <  1:477726/150‑1 (MQ=255)
                                                                                                                                                    aCATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAg  <  2:846949/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAG  >  NC_000913/1620075‑1620372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: