Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,438 A→G 5.3% S790S (TCA→TCG ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,4380AG 5.3% 382.1 / 9.4 133S790S (TCA→TCGynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (62/62);  new base G (3/4);  total (66/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-02

Only 100 of 137 total aligned reads displayed.

GGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAA  >  NC_000913/1660289‑1660587
                                                                                                                                                     |                                                                                                                                                     
ggCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca                                                                                                                                                       <  1:1519821/150‑1 (MQ=255)
   gAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcacca                                                                                                                                                    <  2:64678/150‑1 (MQ=255)
         cGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCg                                                                                                                                              >  2:1835933/1‑150 (MQ=255)
            cTACCAGCCAAAGTAACACCACTTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                           <  1:831485/150‑1 (MQ=255)
            cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGCGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGGGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                           >  2:1577207/1‑150 (MQ=255)
              aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAggg                                                                                                                                         <  1:1417186/150‑1 (MQ=255)
              aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCGGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTCTCCCCTCTGCGCCCTTCACCACTGGCGAAggg                                                                                                                                         >  1:1032058/1‑150 (MQ=255)
               ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGCTAAAATCGCCCATGGCGGCGGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGATGGGa                                                                                                                                        >  1:1470172/1‑150 (MQ=255)
               ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGTATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                        <  1:1709205/150‑2 (MQ=255)
               ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                        <  1:927535/150‑2 (MQ=255)
                 aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                      >  2:1808027/1‑150 (MQ=255)
                 aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTAGGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                      <  2:1364880/150‑1 (MQ=255)
                  gCCAAAGTAAGACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGCAGCCTGGCACGAGGCCAATATGTCTGCCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTACACACTCTGCTCCCTTCAGCACTGGCGAAGGGactc                                                                                                                                     >  1:326841/1‑147 (MQ=255)
                   ccAAAGTAACAGCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCGGGGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggcgcag                                                                                                                                    <  1:936682/150‑6 (MQ=255)
                      aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGGCCATGGCGGCTGTGTGAATACGCTACCCACTCTGCGCCCTGCACCCCTGGCGAAGGGACCCCCgc                                                                                                                                 >  2:381792/1‑150 (MQ=255)
                       aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcc                                                                                                                                >  2:163935/1‑149 (MQ=255)
                       aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgca                                                                                                                                <  1:244987/150‑1 (MQ=255)
                        ttAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcag                                                                                                                               <  1:699698/149‑1 (MQ=255)
                           acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac                                                                                                                            <  2:756748/150‑1 (MQ=255)
                              ccacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt                                                                                                                         >  2:484140/1‑150 (MQ=255)
                              ccacGTATTCTCCCTGGAGATAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACt                                                                                                                         >  1:569145/1‑150 (MQ=255)
                                    aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTg                                                                                                                   >  1:78221/1‑150 (MQ=255)
                                       ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCAAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGTGAAACCCGCAGCACACTAATCTGGTc                                                                                                                <  2:1476974/150‑1 (MQ=255)
                                        tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                               >  1:1400488/1‑150 (MQ=255)
                                        tcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCg                                                                                                               >  1:81165/1‑150 (MQ=255)
                                          ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                                                                                                             <  2:1376845/150‑1 (MQ=255)
                                          ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                                                                                                             <  2:914054/150‑1 (MQ=255)
                                             ggAGTTAGCGCTATGGGCCAGGGAGCCTGTCACGCGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGACGTGACACCCGCAGCACACTAATCTGGTCGAGCTc                                                                                                          >  1:1327178/1‑150 (MQ=255)
                                             ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATc                                                                                                          <  1:418959/150‑1 (MQ=255)
                                              gAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGATGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCg                                                                                                         <  2:44270/150‑1 (MQ=255)
                                              gAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCg                                                                                                         <  1:1056791/150‑1 (MQ=255)
                                                  tAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaa                                                                                                     <  2:1557971/150‑1 (MQ=255)
                                                  tAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaa                                                                                                     >  1:1729418/1‑150 (MQ=255)
                                                    gcgcTATGGGCGAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                   <  2:527304/150‑1 (MQ=255)
                                                    gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                   >  2:1618735/1‑150 (MQ=255)
                                                      gcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTGGCGACGGGAAACCCGCAGCGCACTAATCTGGTCGAGATAGAAAAAata                                                                                                 >  1:209093/1‑150 (MQ=255)
                                                      gcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAata                                                                                                 <  2:766879/150‑1 (MQ=255)
                                                        tATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAAtata                                                                                               <  2:521349/150‑1 (MQ=255)
                                                          tGGGCCAGGGAGCCTGGCACGATGCCAATATGTCTGGCGATAAAATCGACCATGGCTGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAc                                                                                             <  2:1697562/150‑1 (MQ=255)
                                                            ggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAccc                                                                                           <  1:651066/150‑1 (MQ=255)
                                                             gCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATATCCCa                                                                                          >  2:124745/1‑150 (MQ=255)
                                                                   gAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACaa                                                                                    >  2:1384362/1‑150 (MQ=255)
                                                                   gAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACaa                                                                                    >  1:584382/1‑150 (MQ=255)
                                                                    aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc                                                                                   <  2:1430709/150‑1 (MQ=255)
                                                                       cTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCATCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCAt                                                                                >  2:1120808/1‑150 (MQ=255)
                                                                        tGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATa                                                                               <  2:931177/150‑1 (MQ=255)
                                                                         ggCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCCCTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACAATaa                                                                              >  2:189770/1‑150 (MQ=255)
                                                                              gAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCt                                                                         >  1:1970168/1‑150 (MQ=255)
                                                                               acgCCAATATGTCTGGCGATAAAATCGACCATTGCGGCTGTGTGAATACTCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTATTCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTg                                                                        <  1:277508/148‑1 (MQ=255)
                                                                                ggCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTgg                                                                       >  2:1714451/1‑150 (MQ=255)
                                                                                 gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc                                                                      <  2:1046542/150‑1 (MQ=255)
                                                                                 gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc                                                                      <  1:1033807/150‑1 (MQ=255)
                                                                                 gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc                                                                      <  1:484140/150‑1 (MQ=255)
                                                                                  ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAACCCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa                                                                     >  1:2307/1‑150 (MQ=255)
                                                                                    aaTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCagg                                                                   >  2:1807549/1‑148 (MQ=255)
                                                                                    aaTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATg                                                                   <  1:1808028/150‑1 (MQ=255)
                                                                                     atatGTCTGGCGATAAAATCGTCCATGGTGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGa                                                                  <  2:266494/150‑1 (MQ=255)
                                                                                         gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt                                                              >  1:1963759/1‑150 (MQ=255)
                                                                                           cTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAATCCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAGCCCACGCCAACCATAAATTCTGGAATGACAtttt                                                            >  1:1283337/1‑150 (MQ=255)
                                                                                           cTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtttt                                                            <  2:1892899/150‑1 (MQ=255)
                                                                                               cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCAGGGGCGAAGCGAAACCCGCAGCACACTAAGCGGGTCGAGAACGAAAAAATATAACCCACGACAACAATAATTTCGGGCAAGACATtttgttt                                                        >  2:1846068/1‑150 (MQ=255)
                                                                                               cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt                                                        <  1:743821/150‑1 (MQ=255)
                                                                                                taTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                       <  2:2029102/149‑1 (MQ=255)
                                                                                                 aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa                                                      <  1:470803/150‑1 (MQ=255)
                                                                                                 aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa                                                      >  1:1513407/1‑150 (MQ=255)
                                                                                                   aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGACACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                    >  2:1291174/1‑150 (MQ=255)
                                                                                                   aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                    >  1:1748117/1‑150 (MQ=255)
                                                                                                   aaaaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa                                                    <  1:779411/150‑1 (MQ=255)
                                                                                                     aaTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACAATAAATTCTGGCATGACATTTTGTTTGTAAAg                                                  >  2:359491/1‑150 (MQ=255)
                                                                                                      aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc                                                 <  2:774535/150‑1 (MQ=255)
                                                                                                      aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc                                                 <  1:1611406/150‑1 (MQ=255)
                                                                                                       tCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCa                                                <  2:1992775/150‑1 (MQ=255)
                                                                                                        cGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCaa                                               >  1:1839397/1‑150 (MQ=255)
                                                                                                         gACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAAt                                              >  1:636382/1‑150 (MQ=255)
                                                                                                                gcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAg                                       <  2:1400487/150‑1 (MQ=255)
                                                                                                                 cggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGt                                      >  1:648590/1‑150 (MQ=255)
                                                                                                                  ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTa                                     >  1:1477565/1‑150 (MQ=255)
                                                                                                                       tgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatgat                                >  2:1037492/1‑150 (MQ=255)
                                                                                                                          gAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGaa                             <  1:1107562/150‑1 (MQ=255)
                                                                                                                              aCGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTAGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATc                         <  1:342215/150‑1 (MQ=255)
                                                                                                                               cGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCAACGACAACCATAAATTCTGGCTGGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATcc                        >  1:862846/1‑150 (MQ=255)
                                                                                                                                gCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCa                       <  1:449057/150‑1 (MQ=255)
                                                                                                                                  tAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATa                     >  1:791348/1‑150 (MQ=255)
                                                                                                                                   aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                    >  2:957523/1‑150 (MQ=255)
                                                                                                                                   aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                    >  2:919574/1‑150 (MQ=255)
                                                                                                                                   aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                    >  2:900953/1‑150 (MQ=255)
                                                                                                                                    aCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAcc                   <  1:957524/150‑1 (MQ=255)
                                                                                                                                      cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACcac                 >  1:20503/1‑150 (MQ=255)
                                                                                                                                      cACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACcac                 <  1:628354/150‑1 (MQ=255)
                                                                                                                                         tctTCGCCCTTCACCACTTGCGAAGGGAAACCAGCAGCACAATAATCTGGTCGAGATCGAAATAATATAACCGACGACAACCATAAATTCTGGCATGACATTTTGTTTGAACAGCAATAAGTGAGTAATGATGAAAATCCATACCACaga              <  1:378256/150‑1 (MQ=255)
                                                                                                                                            gcgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc           <  2:584382/150‑1 (MQ=255)
                                                                                                                                             cgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAACAATATAACCCACGACAACCATAAATTCTGGCTTGCCAGTTTGTTTGAAAAGAAATAAGTGAGTAATGATGAAAATCCAAACCACAGAGgcg          >  1:345299/1‑150 (MQ=255)
                                                                                                                                              tcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGCGgcgc         >  2:440506/2‑150 (MQ=255)
                                                                                                                                               cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCt        >  2:1586764/1‑150 (MQ=255)
                                                                                                                                               cccTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACAACAGAGGCGCt        >  1:623676/1‑150 (MQ=255)
                                                                                                                                                ccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACACACGACAACCATAAATTCTGGCATGACATTTTGTTTCAAAAGAAATAAGTGAGTAATGATGAAAATCAATACCACAGAGGCGCta       >  1:648979/1‑149 (MQ=255)
                                                                                                                                                 cTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa      >  1:1379832/1‑150 (MQ=255)
                                                                                                                                                  ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt     <  1:2000672/150‑1 (MQ=255)
                                                                                                                                                   tCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATg    <  2:1970167/150‑1 (MQ=255)
                                                                                                                                                    aaccacTGGCGAAGGGAAACCCGCAGCACACTAATCTTGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa   <  1:381792/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAA  >  NC_000913/1660289‑1660587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: