| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,844 | C→G | 20.7% | A642A (GCC→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,844 | 0 | C | G | 20.7% | 27.7 / 7.2 | 33 | A642A (GCC→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (20/6); new base G (4/3); total (24/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG > NC_000913/1708700‑1708992 | tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCCGCTATTGCCCGCGCCCACGCACAAAACCACGAACAGCAGAAGGCTAAAAACGAGcc > 2:904526/1‑150 (MQ=14) ttaccGTGCCAAATCGCGCAAGCTGGAACAGCAACAGCCTACTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa < 1:1132256/147‑1 (MQ=11) ggCCACAGGGCGCAAGCTGGAACAGCATCAGGCTAATGCGGAACCAGAACCCCTGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAacaaca < 2:1941206/149‑1 (MQ=11) acatctcgcAAGCTGGAAGAGCAACAGCCTAATTCGGAACCAGAAGAACATGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 1:620786/144‑1 (MQ=14) aaaTCGCTCAAGATGGTACAGTAACAGTCTTTTGCTGAACCAGCATATCAGGTTGATCCTCGCAAATCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 2:1541432/150‑1 (MQ=11) cgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCACACGCCCGCAAGCGGGCCCCGCACCAGGGTAATGCCGAGCCAGAACTCCAGGTCGATc > 2:1823845/1‑150 (MQ=14) gCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCTCAATTGCCCGTGCCAAAGCACACAACCTGGAACAGCACCAGGCTAAAGCCGAGCCAGAACATCAGGTACATAAGCGCATAGcggccgg > 1:635439/1‑149 (MQ=14) ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGGACCGCAACAGCCAACGGCGGAGCCCGAGGCACGGGGCGATCCGCGCAAAGCTGACGTCGAAGCAGCTAATGc > 1:1054732/1‑150 (MQ=11) aaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTGGATCCGCGCAAAGCCGACGTCGAACCCGCTAGTGCGCGAGCGAAAg > 1:1901651/1‑150 (MQ=11) gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACCCAAGCTCGACCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTATAACCGCGCAAATCCGCAGTTTCCGCCGCTATTGACCGCGCCAACGCGCGaaaa > 2:1645052/1‑150 (MQ=14) gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGACCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGCTACGCGCAAAGCGGCCGTCGCAGCGGCTATTGCCCGCGCCACAGCCCGAAAGCGCGa > 1:1329427/1‑150 (MQ=11) cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTGGATCAGCGCAAAGCCGCCGTCGAAGCGGCTTTTGCGCGAGCCAAAGCGCTCAACCGGGAACAGCAAAAGGCTAATGCggg > 1:1791836/1‑149 (MQ=14) gCTATTGCCCGTGCCAAAGCACGCAATCTGGAACAGCATCAGCCTAATCCCGACCCAGAACAACAGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAg < 1:663115/150‑1 (MQ=17) tGCCCGTGCCACAGCACGCAATCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga < 1:1518968/150‑1 (MQ=255) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTCGACGCGGCTATTGCCCGAGCACAAGCGAGCATACGGGCACAGCAGACGGGTTTTGCGGAGCCagaa > 2:721071/1‑150 (MQ=17) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCCAACGCGCGCAAACGGGACCAGCACCCGGCTCATGCCGAGCCcgaa > 1:1489903/1‑150 (MQ=14) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGACGCCGCTATTGCCCGAGCGCAGGCGCGCAAACCGGAACAGCACCCGGCTTATGCGGAGCCcgaag > 1:273328/1‑150 (MQ=17) cAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGAAAACGGGAACAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTAGAACcgcc > 1:1775355/1‑149 (MQ=14) aGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGATCAGGTTGATCCGCGCa > 1:670743/1‑150 (MQ=255) cTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCCCAAGCGCGCAAACGGGAACAGCAGCAGGCTAATGCCGAGCCAGAAGAGCAGGTGGCTCCCCGCaaa > 1:1944500/1‑150 (MQ=14) tGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCAAAAGCCAAAAAACGCGAACAGCACCAGGATAAACCCGAGCCCTCATAACAGGATCATCCTCTCAAAg > 2:909527/1‑150 (MQ=2) aaCAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc < 2:138230/99‑1 (MQ=255) aaCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc > 1:138230/1‑99 (MQ=16) aaCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGCGAAAACGCGCACAGCACCAGGATAATGCCGAGCAAGAAGAGCAGTTTGATACGCGGAAATCTg > 2:1816360/1‑150 (MQ=14) aCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGCGCCAAAGCCAGCAAACGGGAACAGCAACCGGCTAATACGGAGCCAGAAAAACAGGTAAATCAGCGAAACGCAGc > 1:1360393/1‑150 (MQ=14) cAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCCGCCGCTATTGCCCGCGCCAAAGCCAAAAAACGGGACCAGCAACCGGATGTTGCGGAGCCAGAAGACCATGTTGAACCGCGCAACGcgccc > 1:1297887/1‑146 (MQ=14) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCAAAAGCGCGCAAACAGGAACAGCAACCGGCAAATGCGGAGCCAGAAGAACAGCTTGCTCCGCGCAAAGCTGCCGt > 1:1502335/1‑150 (MQ=14) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCAGTAGACGCCGCTATTGCCCGAGCCAAAGCCCGCAAACGCGAACAGCAACAGGGAAAGGCGGAGCCAGAAGATCAGTTTGATACGAGCAAAGcgtcagg > 2:1873980/1‑144 (MQ=14) aCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCCGCAAACGCGAACAGCAACAGGCTAATGCGGTGCCAGAAGAACAGGTTGCTCCGCGCAACGCTGCAGTCgc > 1:700751/1‑149 (MQ=14) ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc > 1:794144/1‑89 (MQ=255) ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc < 2:794143/89‑1 (MQ=255) ggCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCTCAAACGGGAACAGCAACAGGATAATCCGGAGCAAGAAGAAAAGGTTGATCCGCGAAAAGCTGCAGtagccgc > 2:1008035/1‑144 (MQ=14) aaaTGCGGAAGCAGCACACCAGGCCGATCCGCGCAAACCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAATCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 2:1817469/149‑1 (MQ=11) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg > 1:1145999/1‑150 (MQ=255) | TATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG > NC_000913/1708700‑1708992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |