BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,747,299	A→T	5.4%	I57F (ATT→TTT)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,299	0	A	T	5.4%	323.7 / 2.8	105	I57F (ATT→TTT)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (60/39); new base T (3/3); total (63/42)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTC  >  NC_000913/1747150‑1747448

tgGCTGCCGGGCCGACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAata                                                                                                                                                       <  1:1547891/150‑1 (MQ=255)

  ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGAGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATAtt                                                                                                                                                     >  1:228334/1‑150 (MQ=255)

   gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTg                                                                                                                                                    <  1:1223236/150‑1 (MQ=255)

   gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGAGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTg                                                                                                                                                    >  2:1992496/1‑150 (MQ=255)

    tGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGc                                                                                                                                                   <  1:1480726/150‑1 (MQ=255)

     gCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGcc                                                                                                                                                  <  1:1690927/150‑1 (MQ=255)

      ccGTGCCTACGGGGAGCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTGCTATCTTCGAGCAGGCTGACGAGGCCGGTGGCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCa                                                                                                                                                 <  1:1542349/150‑1 (MQ=255)

      ccGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGccc                                                                                                                                                 >  1:2045454/1‑149 (MQ=255)

        gtgacTACGGTGATCTACACCTTATTCTAGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGt                                                                                                                                               <  2:1832679/146‑1 (MQ=255)

          gCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAATATTTGCCAGCAt                                                                                                                                             <  1:436467/150‑1 (MQ=255)

          gCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAATATTTGCCAGCAt                                                                                                                                             <  2:700766/150‑1 (MQ=255)

            cTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTg                                                                                                                                           <  2:842704/150‑1 (MQ=255)

             tACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAAGAGGCCGGTGTCGGGATGACATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGa                                                                                                                                          <  2:1002169/150‑1 (MQ=255)

              aCGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAATATTTGCCAGCAtcatt                                                                                                                                         <  1:2049674/150‑5 (MQ=255)

               cGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGaaa                                                                                                                                        >  2:282696/1‑150 (MQ=255)

                     tCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATAccgc                                                                                                                                  >  2:697220/1‑150 (MQ=255)

                       tACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATAccgccg                                                                                                                                >  1:815917/1‑150 (MQ=255)

                       tACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATAccgccg                                                                                                                                >  1:1551555/1‑150 (MQ=255)

                         cacCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGAt                                                                                                                              >  2:966759/1‑150 (MQ=255)

                            cTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCTAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTa                                                                                                                           <  2:824781/150‑1 (MQ=255)

                             ttATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTAt                                                                                                                          <  2:1797828/150‑1 (MQ=255)

                              tATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTAtt                                                                                                                         >  2:1568870/1‑150 (MQ=255)

                              tATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTAtt                                                                                                                         >  1:181019/1‑150 (MQ=255)

                                   tcGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACg                                                                                                                    <  1:1981101/150‑1 (MQ=255)

                                   tcGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACg                                                                                                                    <  2:357241/150‑1 (MQ=255)

                                    cGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGGGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGt                                                                                                                   <  2:1306640/150‑1 (MQ=255)

                                     gCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGTGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTa                                                                                                                  <  1:1352965/150‑1 (MQ=255)

                                     gCTTCTACAGAAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGTAAACTCAAAAATGATGCTGGCAAATTTTGCCAGTATTGAAATACCGCCGATTTATTGTAAGTa                                                                                                                  >  2:463775/1‑150 (MQ=255)

                                       ttCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTAtc                                                                                                                <  1:1257898/150‑1 (MQ=255)

                                          tACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCg                                                                                                             >  2:733465/1‑150 (MQ=255)

                                            cAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGaa                                                                                                           >  2:79449/1‑150 (MQ=255)

                                            cAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGaa                                                                                                           >  1:1590012/1‑150 (MQ=255)

                                             aGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAAt                                                                                                          >  2:1831104/1‑150 (MQ=255)

                                              gCAACAAACTCCACTTTATATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCAGACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGGATTGAAATACCGCCGATTTATTGTACGTATCTCTAATg                                                                                                         >  1:1011963/1‑150 (MQ=255)

                                               caacaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTC‑GGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGc                                                                                                       >  2:519021/1‑150 (MQ=255)

                                                 acaaACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCt                                                                                                      >  1:652678/1‑150 (MQ=255)

                                                  caaACTCCACTTTCTATTTCTATCATCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTa                                                                                                     <  1:1527491/150‑1 (MQ=255)

                                                       tCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACaaaa                                                                                                >  1:778454/1‑150 (MQ=255)

                                                       tCCACTTTATATTTCTATCTTCGAGCAGGCTGACGAGGCTGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACaaaa                                                                                                <  2:1333724/150‑1 (MQ=255)

                                                        ccACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAg                                                                                               >  1:840576/1‑150 (MQ=255)

                                                        ccACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGAAGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAg                                                                                               <  1:346185/150‑1 (MQ=255)

                                                          aCTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCa                                                                                             >  1:1517988/1‑150 (MQ=255)

                                                            tttctatttctaTCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaag                                                                                           <  1:474906/150‑1 (MQ=255)

                                                            tttctatttctaTCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGAGGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACAGCGGATGTATTGTACGTATGTCGAATGGCTACAAAAGCagg                                                                                           >  2:1309723/1‑148 (MQ=255)

                                                             ttctatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCaaga                                                                                          <  1:1169471/150‑1 (MQ=255)

                                                                tatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGATAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTTTCTCGAATGGCTACAAAAGCaacaagc                                                                                       >  1:1109748/1‑149 (MQ=255)

                                                                tatttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAagaaga                                                                                       >  1:1759643/1‑150 (MQ=255)

                                                                 atttctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGAc                                                                                      <  1:900408/150‑1 (MQ=255)

                                                                     ctatCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGcc                                                                                  >  2:1486472/1‑150 (MQ=255)

                                                                       atCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCAt                                                                                >  2:1634782/1‑150 (MQ=255)

                                                                        tCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCAtc                                                                               >  1:1677280/1‑150 (MQ=255)

                                                                         cTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCAtct                                                                              >  2:238382/1‑150 (MQ=255)

                                                                          ttCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCAtctc                                                                             >  2:644413/1‑150 (MQ=255)

                                                                           tCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTcc                                                                            >  2:1253433/1‑150 (MQ=255)

                                                                              aGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGc                                                                         >  2:861113/1‑150 (MQ=255)

                                                                                  ggCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTa                                                                     >  2:1102255/1‑150 (MQ=255)

                                                                                  ggCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTAGTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTa                                                                     >  1:1419853/1‑150 (MQ=255)

                                                                                    cTGCCGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCTGTATTGAAATACCGCCGATTTATTGTACGTATCTCCAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGGTATg                                                                   >  2:1444667/1‑150 (MQ=255)

                                                                                    cTGACGAGTCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATg                                                                   <  2:900185/150‑1 (MQ=255)

                                                                                    cTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATg                                                                   <  1:282696/150‑1 (MQ=255)

                                                                                      gACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGc                                                                 >  1:125797/1‑150 (MQ=255)

                                                                                       aCGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCg                                                                <  1:824758/150‑1 (MQ=255)

                                                                                        cGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGt                                                               >  1:627699/1‑150 (MQ=255)

                                                                                          aGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATAATGCCAGTATTGAAATACCGCCGATTTATTGTACGTAGCTCGACTGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTa                                                             <  1:1842124/150‑1 (MQ=255)

                                                                                           ggCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTaa                                                            >  1:1863864/1‑150 (MQ=255)

                                                                                             ccGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAATACAGCCATCTCCAGCGTTATGGCGTTaaaa                                                          >  2:1227252/1‑150 (MQ=255)

                                                                                             ccGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTaaaa                                                          >  2:587566/1‑150 (MQ=255)

                                                                                             ccGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTaaaa                                                          >  2:579655/1‑150 (MQ=255)

                                                                                              cGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTaaaaa                                                         <  1:938270/150‑1 (MQ=255)

                                                                                                gtgtCGTGATGCCATACAGTGATGAGTAAAACTCAAAAATGATGCTTGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACATCCATCTCCAGCGTTATGGCGTTAAAAAAg                                                       <  1:364594/150‑1 (MQ=255)

                                                                                                    cGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAAc                                                   <  1:719465/150‑1 (MQ=255)

                                                                                                    cGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAAc                                                   >  1:788863/1‑150 (MQ=255)

                                                                                                    cGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAAc                                                   >  1:561630/1‑150 (MQ=255)

                                                                                                     gggATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAAcc                                                  >  2:723404/1‑150 (MQ=255)

                                                                                                         tGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTGTTGGCGTTAAAAAAGAAACCTTGc                                              >  2:121053/1‑150 (MQ=255)

                                                                                                          gCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCa                                             >  1:1475354/1‑150 (MQ=255)

                                                                                                           ccATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCAc                                            <  1:605831/150‑1 (MQ=255)

                                                                                                           ccATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCAc                                            >  2:690527/1‑150 (MQ=255)

                                                                                                            cATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACAGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACg                                           >  2:122916/1‑150 (MQ=255)

                                                                                                                ccgTGATGAGGAAAACTCAAAAAAGAAGCAGGCAAATATTGCCAGTATTGAAATCCCGCCGAGATATTGTACGTCTCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTAATGGCGTTAAAAAAGAAACCTTGCACGATCg                                       <  2:1228345/148‑1 (MQ=255)

                                                                                                                    gatgaGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAg                                   >  2:120523/1‑150 (MQ=255)

                                                                                                                       gaGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGttt                                <  1:1402494/150‑1 (MQ=255)

                                                                                                                         ggAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGttttt                              >  1:1239529/1‑150 (MQ=255)

                                                                                                                               cTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACcgcg                        >  2:1767818/1‑150 (MQ=255)

                                                                                                                                tCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGAACGATCGTCAGTTTTTACCGCGa                       >  1:268456/1‑150 (MQ=255)

                                                                                                                                 cAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGaa                      >  1:762119/1‑150 (MQ=255)

                                                                                                                                  aaaaaTGATGCTGTCAAATATTGCAAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGATACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAAt                     <  1:987156/150‑1 (MQ=255)

                                                                                                                                       caatacTGGCAATATTTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGAACGTCAGTTTTTACCGCGAATTctgc                >  2:476544/6‑150 (MQ=255)

                                                                                                                                        ttagCTCGCATATATTGCCAGTATTTAAATACAGACGATTTATTGTACGTATCTCGAATGGATACAAAAGCAAGAATACAGCAATCTCAAGCGTTATGGCGTTAAAAAAGAAACATTGCACGATCGTCAGTTTTTACCGCGAATTctgct               <  2:381781/147‑1 (MQ=255)

                                                                                                                                        tatgCTGGCAAATATTGCCAGTATTGAATTACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTctgct               <  1:1011502/149‑1 (MQ=255)

                                                                                                                                         atacTGGCAATATTTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTctgctg              >  2:2013619/4‑150 (MQ=255)

                                                                                                                                            cTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGc           <  1:1214556/150‑1 (MQ=255)

                                                                                                                                             tGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCg          >  1:1675018/1‑150 (MQ=255)

                                                                                                                                              ggCAAATATTGCCAGTATTGAAATACAGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGAGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGa         >  1:1210855/1‑150 (MQ=255)

                                                                                                                                               gCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGaa        <  2:1930440/150‑1 (MQ=255)

                                                                                                                                                cAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGCCAGCCATCTCCCGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAat       >  1:1502851/1‑150 (MQ=255)

                                                                                                                                                cAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAat       <  1:1477541/150‑1 (MQ=255)

                                                                                                                                                aaaaTATTGCCTGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAat       <  1:707178/149‑1 (MQ=255)

                                                                                                                                                  aaTATTGCCAGTATTGCAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAATGCAAGAAGACAGCCATATCCAGCGTTATGGCGTAAAAAAAGAAACATTGCACGATCGTCAGTATTTCACGCGAATTCTGCTGGGCGAagat     >  1:119177/1‑150 (MQ=255)

                                                                                                                                                     atTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCATCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTc  <  1:1419905/150‑1 (MQ=255)

TGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCCAGTATTGAAATACCGCCGATTTATTGTACGTATCTCGAATGGCTACAAAAGCAAGAAGACAGCCATCTCCAGCGTTATGGCGTTAAAAAAGAAACCTTGCACGATCGTCAGTTTTTACCGCGAATTCTGCTGGGCGAATATTTC  >  NC_000913/1747150‑1747448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`