Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,816,590 C→A 5.4% L182L (CTG→CTT)  chbG ← chito‑oligosaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,816,5900CA 5.4% 344.4 / 2.8 114L182L (CTG→CTT) chbGchito‑oligosaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base C (54/52);  new base A (2/6);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTG  >  NC_000913/1816442‑1816739
                                                                                                                                                    |                                                                                                                                                     
   acgCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCg                                                                                                                                                   <  1:665801/150‑1 (MQ=255)
      cTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACCGCGAcg                                                                                                                                                >  1:918427/1‑149 (MQ=255)
      cTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACt                                                                                                                                                >  1:1695632/1‑150 (MQ=255)
       tGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTc                                                                                                                                               >  2:1430940/1‑150 (MQ=255)
         aTGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCAc                                                                                                                                             <  2:1626057/150‑1 (MQ=255)
          tGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCCGCGTCATCCAGCACTTGCAGGAACAGCGACTCACt                                                                                                                                            <  1:1815612/150‑1 (MQ=255)
           gTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTg                                                                                                                                           <  2:1197492/150‑1 (MQ=255)
            tCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGa                                                                                                                                          >  1:895499/1‑150 (MQ=255)
             cAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGAt                                                                                                                                         >  2:637455/1‑150 (MQ=255)
             cAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGAt                                                                                                                                         >  2:1119602/1‑150 (MQ=255)
             cAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGAAGGAACAGCGACTCACTGAt                                                                                                                                         >  2:1698533/1‑150 (MQ=255)
              aaCACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGAtc                                                                                                                                        <  2:673787/150‑1 (MQ=255)
              aaCACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGAtc                                                                                                                                        >  2:19739/1‑150 (MQ=255)
              aaCACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGAtc                                                                                                                                        >  1:1445504/1‑150 (MQ=255)
                     ccAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCAc                                                                                                                                 >  2:1794710/1‑150 (MQ=255)
                     ccAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCAc                                                                                                                                 >  1:609372/1‑150 (MQ=255)
                      cAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCAcc                                                                                                                                <  2:1498707/150‑1 (MQ=255)
                         cTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTa                                                                                                                             >  2:51081/1‑150 (MQ=255)
                           ccGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGa                                                                                                                           >  1:1119415/1‑150 (MQ=255)
                            cGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGCCACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGaa                                                                                                                          >  2:1275070/1‑150 (MQ=255)
                            cGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATTTATTTTTTTAATGATAAGGGTATCACTGATATCTTCACCGTAGaa                                                                                                                          <  2:642147/150‑1 (MQ=255)
                            cGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGaa                                                                                                                          >  2:1938352/1‑150 (MQ=255)
                              ttAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATg                                                                                                                        <  2:729767/150‑1 (MQ=255)
                                aaGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTCATCTCTTCACCGTAGAATGCa                                                                                                                      >  2:27155/1‑150 (MQ=255)
                                  gCGTGGGAAACAGTAAGCACTCTGACGGATAGTTTTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGACCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCAct                                                                                                                    <  2:391583/150‑1 (MQ=255)
                                    gTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCActgc                                                                                                                  >  2:1833997/1‑150 (MQ=255)
                                    gTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCActgc                                                                                                                  >  1:1111023/1‑150 (MQ=255)
                                       ggAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGa                                                                                                               >  2:1847648/1‑150 (MQ=255)
                                       ggAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGa                                                                                                               >  2:264808/1‑150 (MQ=255)
                                         aaaCAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAAt                                                                                                             >  1:1355813/1‑150 (MQ=255)
                                          aaCAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATc                                                                                                            >  1:670448/1‑150 (MQ=255)
                                             aGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCt                                                                                                         <  1:1947458/150‑1 (MQ=255)
                                              gTAAGCACTCTGACGGATAGTATTATCGATAATCGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTg                                                                                                        <  1:152692/150‑1 (MQ=255)
                                               tAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTgg                                                                                                       <  2:629948/150‑1 (MQ=255)
                                                aaGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTGACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTggg                                                                                                      <  2:1107023/150‑1 (MQ=255)
                                                 aGCACTCTGACGGATAGTATTATCGATAAACCCCGGATGACACATCACCGCCAGCGCACGATCACCCCGATGTCCTTCGTCATTTTTTTTTTTAATGATAAGGGTATCACTGAGATCTTCACCGTAGAATGCACTGCTGAATCCCTGGGt                                                                                                     <  2:1397513/150‑1 (MQ=255)
                                                  gCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTa                                                                                                    >  1:1174848/1‑150 (MQ=255)
                                                   cACTCTGCCGTATAGTATTATCGATAAACTCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACGGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAg                                                                                                   <  1:1484945/150‑1 (MQ=255)
                                                     ctctGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTg                                                                                                 <  2:1620637/150‑1 (MQ=255)
                                                         gACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCa                                                                                             >  1:1007935/1‑150 (MQ=255)
                                                         gACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCa                                                                                             <  1:886509/150‑1 (MQ=255)
                                                           cGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAgg                                                                                           <  1:971720/150‑1 (MQ=255)
                                                           cGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAgg                                                                                           >  1:541350/1‑150 (MQ=255)
                                                                 gTATTATCGATAAACGCCGGATGACACCTCACCTCCAGCGAACGCTCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGAcc                                                                                     <  1:508496/150‑1 (MQ=255)
                                                                  tattatCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGACAAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAAGCCGTGGGTAGTGCGCAGGTTGACCg                                                                                    >  1:841198/1‑150 (MQ=255)
                                                                       tCGATAAACGCCGGATGACACATCACCTACAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGAACAGCTGAATCCCTCGGTACTGCGGAGGTTGACCGGCaaa                                                                               >  1:1092186/1‑150 (MQ=255)
                                                                        cGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCTTCATCCATCATTTTCAGGAAAAGCGAATCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAAt                                                                              <  2:1487339/150‑1 (MQ=255)
                                                                        cGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCatat                                                                              >  1:1330156/1‑150 (MQ=255)
                                                                        cGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAAt                                                                              <  2:1468138/150‑1 (MQ=255)
                                                                         gATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATc                                                                             >  1:522644/1‑150 (MQ=255)
                                                                         gATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATc                                                                             <  1:500515/150‑1 (MQ=255)
                                                                         gATAAACGCCGGATGACACATCACATCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCACTTCACCGTAGACTGCACTGCTGAAGCCCTGGGTACTGCGCAGGTTGACCgcaataac                                                                             >  1:402515/1‑143 (MQ=255)
                                                                            aaaCGCCGGATGAAACATCACCTCCAGCGCACGATCACCCCGATGGCCTGAGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTCAATACATGGGTAGTGCGCAGGTTGACCGGCAAATCaaa                                                                          >  1:1408167/1‑150 (MQ=255)
                                                                               cGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGc                                                                       >  1:634543/1‑150 (MQ=255)
                                                                                   ggATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCTACTCACTGATCTCTTCACCGTAGAATTCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATc                                                                   <  1:357125/150‑1 (MQ=255)
                                                                                   ggATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATc                                                                   <  1:226003/150‑1 (MQ=255)
                                                                                   ggATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATc                                                                   <  1:686422/150‑1 (MQ=255)
                                                                                      tGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGa                                                                <  2:1339441/150‑1 (MQ=255)
                                                                                        acacATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACg                                                              >  2:1257128/1‑150 (MQ=255)
                                                                                              cACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATccgc                                                        <  2:1007934/150‑1 (MQ=255)
                                                                                                ccTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGCCTCACTGATCTCTTCACCGTAGACTGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATccgccc                                                      >  2:1582796/1‑150 (MQ=255)
                                                                                                ccTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCATGTTTTTTAATGAAAAGGGTATCACTGATATCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCTAATCAAACGCCATCTGACGATccgccc                                                      <  2:1513676/150‑1 (MQ=255)
                                                                                                     aGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaac                                                 <  2:911117/150‑1 (MQ=255)
                                                                                                      gCGAACGATCACCCCGATTGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacg                                                <  2:1256584/150‑1 (MQ=255)
                                                                                                      gCGAACGATCACCCCGATGGCCTGCGTCATTTTTTTTTTTAATGATAAGGGTATCACTGAGATCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacg                                                <  2:937250/150‑1 (MQ=255)
                                                                                                      gCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacg                                                >  2:1781346/1‑150 (MQ=255)
                                                                                                       cGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacgc                                               <  1:645986/150‑1 (MQ=255)
                                                                                                       cGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacgc                                               <  1:1430941/150‑1 (MQ=255)
                                                                                                       cGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCcgcaacgc                                               <  1:1794711/150‑1 (MQ=255)
                                                                                                           cGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCGCTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATg                                           <  2:1013176/150‑1 (MQ=255)
                                                                                                           cGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATg                                           <  2:769776/150‑1 (MQ=255)
                                                                                                           cGATAACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGAATCACTGATATCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATg                                           <  2:1412216/150‑1 (MQ=255)
                                                                                                            gATCACCCCGATGGCCTGCGTCATCCAGCAGTTGCAGGAACAGCGACTCACTGATCTCTTCCCCGTAGATTGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAACTCAAACGCCATGTGTCGATCCGCCCGCCACGCCATGc                                          >  2:244157/1‑150 (MQ=255)
                                                                                                            gATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGc                                          >  1:883134/1‑150 (MQ=255)
                                                                                                            gATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGc                                          <  1:51081/150‑1 (MQ=255)
                                                                                                            gATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAAAGc                                          >  2:78608/1‑150 (MQ=255)
                                                                                                             aTCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGcc                                         >  2:1433208/1‑150 (MQ=255)
                                                                                                               cACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCt                                       <  2:1895333/150‑1 (MQ=255)
                                                                                                                 ccccGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGc                                     >  1:513893/1‑150 (MQ=255)
                                                                                                                 ccccGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGc                                     >  2:113587/1‑150 (MQ=255)
                                                                                                                 acccGATGTCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTTAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGc                                     <  1:1324563/149‑1 (MQ=255)
                                                                                                                   ccGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGctc                                   <  1:19739/150‑1 (MQ=255)
                                                                                                                        ggCCTGCGGCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCg                              <  1:1446184/150‑1 (MQ=255)
                                                                                                                        ggCATGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCTTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCg                              <  1:1257129/150‑1 (MQ=255)
                                                                                                                          ccTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCa                            <  1:1006874/150‑1 (MQ=255)
                                                                                                                             gCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAAc                         <  2:1445503/150‑1 (MQ=255)
                                                                                                                                 cATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTgg                     <  1:45020/150‑1 (MQ=255)
                                                                                                                                  aTCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAATCCTGGc                    <  2:1639286/150‑1 (MQ=255)
                                                                                                                                  aTCCAGCACATGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGAGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGc                    <  2:1586687/150‑1 (MQ=255)
                                                                                                                                      aGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGcaaca                >  1:384104/1‑150 (MQ=255)
                                                                                                                                          cTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCg            <  2:1695631/150‑1 (MQ=255)
                                                                                                                                           ttGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCgg           <  2:883133/150‑1 (MQ=255)
                                                                                                                                            tGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCggg          >  2:766977/1‑150 (MQ=255)
                                                                                                                                            tGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAAAGCCCTGCTCTGCCGCAAACCTGGCAACAATCggg          >  2:1122459/1‑150 (MQ=255)
                                                                                                                                            tGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGAACCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCggg          >  2:289303/1‑150 (MQ=255)
                                                                                                                                            tGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCAGACGATCCGCCCGCAACGCAATGCCCAGCTCTGCCGCAAACCTGGCAACAATCggg          >  1:1547983/1‑150 (MQ=255)
                                                                                                                                             gCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGa         <  1:357109/150‑1 (MQ=255)
                                                                                                                                              cAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGCTCCGCCCGCACCGCAAAGCCCTGCTCTGCCGCAACCCTGGCAACAATCGGGaa        >  1:868710/1‑150 (MQ=255)
                                                                                                                                               aGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTAGACCGGCAAATCATACGCCATCTGACGATCCGCCCGCAACTCAATGCCCTTCTCTGCCGAAAACCTGGCAAGAATCGGGaaa       <  2:631797/150‑1 (MQ=255)
                                                                                                                                                    cAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCCACGCAATGCCCTGCTCTGCCGCAACCCTGGCCACAATCGGGAAAATCTg  >  2:2001037/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGACGCTGATGTCAACACATCCAGCTCCGTTAAGCGTGGGAAACAGTAAGCACTCTGACGGATAGTATTATCGATAAACGCCGGATGACACATCACCTCCAGCGAACGATCACCCCGATGGCCTGCGTCATCCAGCACTTGCAGGAACAGCGACTCACTGATCTCTTCACCGTAGAATGCACTGCTGAATCCCTGGGTAGTGCGCAGGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTG  >  NC_000913/1816442‑1816739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑