Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,830,060 G→C 5.0% R236G (CGC→GGC)  astA ← arginine succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,0600GC 5.0% 378.5 / 10.4 119R236G (CGC→GGC) astAarginine succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (61/52);  new base C (4/2);  total (65/54)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

Only 100 of 126 total aligned reads displayed.

GCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCC  >  NC_000913/1829911‑1830208
                                                                                                                                                     |                                                                                                                                                    
gCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgcgcg                                                                                                                                                      >  1:2001946/1‑150 (MQ=255)
gCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgcgcg                                                                                                                                                      >  1:452114/1‑150 (MQ=255)
 cccTGCGCAGGCTGCCCTTCTGCCACTTCCATCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTTAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCgg                                                                                                                                                     <  2:730738/150‑1 (MQ=255)
  ccTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCggg                                                                                                                                                    >  1:1278519/1‑150 (MQ=255)
   cTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCTAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                                                                                                   <  2:584812/150‑1 (MQ=255)
      cgcAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAgg                                                                                                                                                <  1:1543902/150‑1 (MQ=255)
          ggCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGCCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCCGGcgcg                                                                                                                                            >  1:1558711/1‑150 (MQ=255)
           gCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCgg                                                                                                                                           <  1:1297358/150‑1 (MQ=255)
            cTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGt                                                                                                                                          >  1:551830/1‑150 (MQ=255)
            cTGCCCTTCTGCCACTTCAACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTTCGGTAGCGAAAAGCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGt                                                                                                                                          >  1:656475/1‑150 (MQ=255)
             tGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCCCGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAACCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCgggt                                                                                                                                         >  2:49175/1‑148 (MQ=255)
               cccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTg                                                                                                                                       <  2:1467833/150‑1 (MQ=255)
               cccTTCTGCCACTTCCACCAGCCGACTTATACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCTATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgct                                                                                                                                       <  2:1287928/150‑4 (MQ=255)
                ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGc                                                                                                                                      >  2:223874/1‑150 (MQ=255)
                 cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgctgg                                                                                                                                     <  2:1950679/150‑6 (MQ=255)
                  ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                                    <  1:1772875/150‑1 (MQ=255)
                     tGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCCCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                                                                                                 >  2:943425/1‑150 (MQ=255)
                      gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                                >  1:369959/1‑150 (MQ=255)
                      gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGGCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                                <  2:35057/150‑1 (MQ=255)
                        cACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAc                                                                                                                              >  1:861502/1‑150 (MQ=255)
                        cACTTCCACAAGCCGACTTATACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCATCAAAGATGTCGATATAGTTACGGAAGCGAAATCCTGCTTACTCCAGCACCGCGCGGGCAGGCGCGGATTGCGGATGTAc                                                                                                                              >  2:1109368/1‑150 (MQ=255)
                         aCTTCCACCAGCCGACTTTTCCGTATGGCGCGCACGCGGTCGATGTCACACTCAAGCGGCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                             <  1:1256677/150‑1 (MQ=255)
                         aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                             <  1:907583/150‑1 (MQ=255)
                         aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                             <  1:668936/150‑1 (MQ=255)
                          cTTCCACCAGCCGACTTTTACGGATGGCGCGCACTCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCt                                                                                                                            <  2:923251/150‑1 (MQ=255)
                           ttCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTg                                                                                                                           <  2:2042073/150‑1 (MQ=255)
                              caccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                                                                                                        <  2:475304/150‑1 (MQ=255)
                              caccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                                                                                                        <  1:1806190/150‑1 (MQ=255)
                              caccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                                                                                                        >  2:596918/1‑150 (MQ=255)
                               accaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGAACCTGACCg                                                                                                                       <  2:1278518/150‑1 (MQ=255)
                                ccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTAGCGGATGTCCCTGACCgc                                                                                                                      >  2:891476/1‑149 (MQ=255)
                                   gCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGa                                                                                                                   <  2:540441/150‑1 (MQ=255)
                                   gCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGa                                                                                                                   <  1:149543/150‑1 (MQ=255)
                                    ccGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGAc                                                                                                                  <  2:519695/150‑1 (MQ=255)
                                      gACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGt                                                                                                                <  2:146952/150‑1 (MQ=255)
                                           ttACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTgg                                                                                                           >  2:1984554/1‑150 (MQ=255)
                                           ttACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTgg                                                                                                           <  1:377438/150‑1 (MQ=255)
                                               ggATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCGGCGTCATAGAAGGCGATATAGTTACGGTAGCGCAAACCTTCTTTCTCCAGCACCGCGCGGGCCGGCGCGGTTTGCGGATGTACCTGACCGCTGACGTCCGGGGcgt                                                                                                       >  1:75914/1‑148 (MQ=255)
                                                 aTGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTcc                                                                                                     <  1:1927137/150‑1 (MQ=255)
                                                   ggCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg                                                                                                   <  2:1524337/150‑1 (MQ=255)
                                                     cgcgcACGCGGTCGATGTCACACTCAAGCGGCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTggg                                                                                                 <  2:1558623/150‑1 (MQ=255)
                                                       cgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAt                                                                                               >  2:1193740/1‑150 (MQ=255)
                                                         cACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATaa                                                                                             >  1:1049575/1‑150 (MQ=255)
                                                         cACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaa                                                                                             >  1:1440350/1‑150 (MQ=255)
                                                           ctcgGTCGATGTCACACTAAAGCGTCGGCCCACCGTCAAAGATGTAGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaa                                                                                           <  2:1441846/148‑1 (MQ=255)
                                                                gcGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg                                                                                      <  2:452114/149‑1 (MQ=255)
                                                                 cGATGTCACACTCACGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                     <  1:616037/150‑1 (MQ=255)
                                                                 cGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGCTGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                     <  2:442415/150‑1 (MQ=255)
                                                                 cGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                     <  1:1226265/150‑1 (MQ=255)
                                                                     gTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGtata                                                                                 >  1:1048576/1‑150 (MQ=255)
                                                                      tCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAg                                                                                >  2:586524/1‑150 (MQ=255)
                                                                         cacacaAGCGTCGGCCCACCGGCAAAGAGGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                                                                             <  2:166263/150‑1 (MQ=255)
                                                                         cacTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                                                                             >  1:292459/1‑150 (MQ=255)
                                                                         cacTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                                                                             >  1:1895761/1‑150 (MQ=255)
                                                                         cacTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATc                                                                             >  1:1686620/1‑150 (MQ=255)
                                                                          acTCAAGCGTCGGCCCACAGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCg                                                                            >  1:350519/1‑150 (MQ=255)
                                                                           cTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCgg                                                                           >  1:1688474/1‑150 (MQ=255)
                                                                             cAAGCGTCGGCCCACCGTCAAAGATGTCGATATACTTACGGAAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTACCTGGGATAAAAAGAGTGTATAGATCGGAt                                                                         >  1:301435/1‑150 (MQ=255)
                                                                               aGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCGTCCTGGGATAAAAAGTGGGTATAGATCGGATGt                                                                       >  1:591461/1‑150 (MQ=255)
                                                                                   tCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATTAAAAGTGGGTATAGATCGGATGTTTCg                                                                   >  1:1314557/1‑150 (MQ=255)
                                                                                    cGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCgg                                                                  <  1:175200/150‑1 (MQ=255)
                                                                                     ggCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGc                                                                 >  1:1877774/1‑150 (MQ=255)
                                                                                     ggCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGc                                                                 <  2:557252/150‑1 (MQ=255)
                                                                                      gCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCa                                                                <  1:579122/150‑1 (MQ=255)
                                                                                      gCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCACGCGCGGTTTGCGGATGTACCTGAGCGATGACGTCCTGGGCTTACGGGGAGAAAAAGTGGGTATAGATGGGATGTTTCGGCa                                                                >  2:1800445/1‑150 (MQ=255)
                                                                                       cccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCAt                                                               >  1:220292/1‑150 (MQ=255)
                                                                                        ccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATc                                                              <  1:223874/150‑1 (MQ=255)
                                                                                         cACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCa                                                             >  1:1267872/1‑150 (MQ=255)
                                                                                           ccGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAGCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGATAAAGAACGATGGTTACGGCAACAGt                                                           >  2:227877/1‑150 (MQ=255)
                                                                                            cGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGAATAGATCGGATGTTTCGGCATCAGtt                                                          >  1:712233/1‑150 (MQ=255)
                                                                                               cAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGCTCGGATGTTTCGGCATCAGTTCTg                                                       >  2:32649/1‑150 (MQ=255)
                                                                                               cAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTg                                                       >  2:700727/1‑150 (MQ=255)
                                                                                                  aGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCaa                                                    >  1:1029687/1‑150 (MQ=255)
                                                                                                   gATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAAt                                                   >  1:1338503/1‑150 (MQ=255)
                                                                                                    aTGTCGATATAGTTACGGTAGGGAAAACCTTCATTCTCCAGCACCGCGCGGGCTGGCGCGGTTTGCGGATGTTCCTGTCCGATGACGTCCTGGGCTTCCTGGGTTAATTTGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCattt                                                  <  2:1773645/150‑4 (MQ=255)
                                                                                                     tGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGCCCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaa                                                 >  2:400367/1‑150 (MQ=255)
                                                                                                        cGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATg                                              >  1:604754/1‑150 (MQ=255)
                                                                                                          atatAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGcc                                            <  1:1104792/150‑1 (MQ=255)
                                                                                                                  aCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGccc                                    <  2:369959/150‑1 (MQ=255)
                                                                                                                  aCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGGGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGccc                                    <  1:806676/150‑1 (MQ=255)
                                                                                                                    ggTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCgg                                  >  1:1462475/1‑150 (MQ=255)
                                                                                                                       aGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGc                               <  2:1048575/150‑1 (MQ=255)
                                                                                                                         cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCTATAAATGCCTTTTGCCCGGTGCCg                             >  2:1484375/1‑150 (MQ=255)
                                                                                                                         cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCg                             <  2:820001/150‑1 (MQ=255)
                                                                                                                          gAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACAGAAGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCAACAGTTCTGCAATAAATGCCTTTTGCCCGGTGccac                            >  1:354353/1‑148 (MQ=255)
                                                                                                                          gAAAACCTTCTTTCTCCAGCACCACGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGc                            >  1:1795265/1‑150 (MQ=255)
                                                                                                                               ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                       >  2:385583/1‑150 (MQ=255)
                                                                                                                                    tttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGCTGTACCTCACCGATGACGTCCTGGGCGTCCTGGGATACAAAGTGGGCATAGATCGCAAGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATc                  <  2:301970/150‑1 (MQ=255)
                                                                                                                                    tttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATc                  >  2:1113296/1‑150 (MQ=255)
                                                                                                                                    tttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATc                  <  2:651585/150‑1 (MQ=255)
                                                                                                                                       ctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAACTGCCTTTTGCCCGGTGCCGCAGAGAAAATCgtc               >  1:300574/1‑148 (MQ=255)
                                                                                                                                        tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg              >  1:977396/1‑150 (MQ=255)
                                                                                                                                        tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg              <  1:1491884/150‑1 (MQ=255)
                                                                                                                                        ccgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg              >  2:1030815/7‑150 (MQ=255)
                                                                                                                                         ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGCTCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc             >  1:258080/1‑150 (MQ=255)
                                                                                                                                          caacACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGcgcg            <  2:1297090/147‑1 (MQ=255)
                                                                                                                                          cAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGcgcg            <  1:963989/150‑1 (MQ=255)
                                                                                                                                           aGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGATGCCGCAGAGAAAATCGGCGCgg           >  1:1043728/1‑150 (MQ=255)
                                                                                                                                                  cgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAAt    <  2:2001945/150‑1 (MQ=255)
                                                                                                                                                   gcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATc   <  1:1984555/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCC  >  NC_000913/1829911‑1830208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: