Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 5.2% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 5.2% 344.9 / 6.3 116intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (52/56);  new base A (2/4);  total (54/62)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 118 total aligned reads displayed.

TATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864611‑1864907
                                                                                                                                                   |                                                                                                                                                       
tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                       <  1:1786352/150‑1 (MQ=255)
tatGCCGGATGATGGCTACAAAACATTTGATTGTGTAGAAACGGCTTACGCTTCAGAAAAGCAAAAAGTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGCGTg                                                                                                                                                       >  1:1556068/1‑150 (MQ=255)
 ttGCCGGATGATGGCTACAAAACATTTGGTTGTGTAGAAACGGCTGACGCTTCAGACACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGATGTGTGCCGGGTGc                                                                                                                                                      <  1:69708/149‑1 (MQ=255)
 atGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACTTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGc                                                                                                                                                      <  2:139570/150‑1 (MQ=255)
 atGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGc                                                                                                                                                      <  2:1820683/150‑1 (MQ=255)
   gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTGCGCTTCAGAAACGCAAAAAGTGACCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                    >  1:45450/1‑150 (MQ=255)
   gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTGCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                    >  1:1483304/1‑150 (MQ=255)
   gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                    >  2:33185/1‑150 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  <  2:1638633/150‑1 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  <  2:1321361/150‑1 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  >  2:1226909/1‑150 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  <  2:1836408/150‑1 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  <  1:1497837/150‑1 (MQ=255)
     cGGATGATGGCTACAAAACATTTGTTTGAGAAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                  <  1:1028478/150‑1 (MQ=255)
         tgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAta                                                                                                                                              >  1:1735393/1‑149 (MQ=255)
           atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                            >  2:534463/1‑150 (MQ=255)
            tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                           <  2:1113142/150‑1 (MQ=255)
            tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                           <  1:505649/150‑1 (MQ=255)
              gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATACGGca                                                                                                                                        >  1:1503447/1‑150 (MQ=255)
               cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                       <  1:1403703/150‑1 (MQ=255)
                tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacc                                                                                                                                      >  2:53061/1‑149 (MQ=255)
                 aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATACATCCGGcacac                                                                                                                                    <  2:1355610/150‑1 (MQ=255)
                 aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                    <  1:550108/150‑1 (MQ=255)
                 aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTAGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacac                                                                                                                                    <  2:1553946/150‑1 (MQ=255)
                    aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTTACCAAAGATAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTTAATGTTGGGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 <  1:865859/150‑1 (MQ=255)
                       cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAta                                                                                                                              >  1:82/1‑149 (MQ=255)
                        aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGATCAATCCATTCGAGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCACGGTTCAATCCATCCGGCACACAACATca                                                                                                                             >  1:130692/1‑150 (MQ=255)
                           tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                          >  1:1172381/1‑150 (MQ=255)
                           tgtatgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATACGCGTTGCGAAACGCTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAttaacg                                                                                                                          <  1:341806/150‑6 (MQ=38)
                            gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                         <  1:1213815/150‑1 (MQ=255)
                            gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGAGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCTATGCATCCGGCACACAACATCACAcc                                                                                                                         <  2:322886/150‑1 (MQ=255)
                               tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                      >  2:1212581/1‑150 (MQ=255)
                                 tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc                                                                                                                    >  2:711291/1‑150 (MQ=255)
                                   tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATACa                                                                                                                  >  1:1938599/1‑150 (MQ=255)
                                   tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCAGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCa                                                                                                                  >  2:224509/1‑150 (MQ=255)
                                     gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                >  2:1408440/1‑150 (MQ=255)
                                       aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAgcgc                                                                                                              >  1:1090724/1‑150 (MQ=255)
                                       aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATACAgcgc                                                                                                              >  2:1688220/1‑150 (MQ=255)
                                               aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCGATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGGTTTTc                                                                                                      <  2:854321/150‑1 (MQ=255)
                                                cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAAAGCATCCCGCACACAACATCACACCATATCCACCGCAGTTTTTcc                                                                                                     >  1:1944383/1‑150 (MQ=255)
                                                 gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                    >  2:1050825/1‑150 (MQ=255)
                                                  cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   >  2:29393/1‑150 (MQ=255)
                                                  cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   <  2:1814357/150‑1 (MQ=255)
                                                  cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAAACGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCtt                                                                                                   >  1:1930348/1‑150 (MQ=255)
                                                     cAGAAGCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                >  2:1432404/1‑150 (MQ=255)
                                                     cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                >  2:1925857/1‑150 (MQ=255)
                                                       gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              >  2:1392083/1‑150 (MQ=255)
                                                          aCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGcc                                                                                           <  1:891864/150‑1 (MQ=255)
                                                           cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                          <  2:411148/150‑1 (MQ=255)
                                                            gCAAAAAGTGACCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                         <  2:1668521/150‑1 (MQ=255)
                                                             cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTTCAAAACATACGGCACACCACATAACACCATATCCAGCGGAGTGTTTCCTTTTGGTGACgct                                                                                        >  2:1525400/1‑148 (MQ=255)
                                                             cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCCATGCATACGGCACACCACATCACCCCATTTCCCGCGGAGGTTTTGCTGTTGGTGCCggg                                                                                        >  2:1981743/1‑149 (MQ=255)
                                                             cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                        <  1:596015/150‑1 (MQ=255)
                                                                   gTGACCATAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATTTTGTGTGCCGGTTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                  <  1:1315087/150‑1 (MQ=255)
                                                                     gACCAAAGCGACACCTGAACATCTGTCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                <  2:1260427/150‑1 (MQ=255)
                                                                      aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                               <  2:1210886/150‑1 (MQ=255)
                                                                       ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTAACGTTAATGTTGTGTGCCGGGTGCCAGGCAACCCGCCCCCCCCAACCCGCCAATTCCCGCGGAGTTTTAGCGTTTGGTGCCGGATTTGCCGTAt                                                                              >  1:1007307/1‑150 (MQ=255)
                                                                       ccAAAGAGAAACATGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTTCAATGAATCAGGGACAACAACTCACACCATATGACTAGCAGTTTTTGCTTTTGGTGACCGATATGCCatat                                                                              >  1:698447/1‑150 (MQ=255)
                                                                        cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                             >  2:1611486/1‑150 (MQ=255)
                                                                         aaaGACAAACCTCCACATCTGGCGCAATCCATTCGCGTGGCTAAACTTAAATTACCTTTAATGTGGTGTGCCGGGGGAATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            <  2:1525084/150‑1 (MQ=255)
                                                                          aaGAGAAACCTGCACATCTTGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                           <  1:66472/150‑1 (MQ=255)
                                                                           agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                          <  2:1127654/150‑1 (MQ=255)
                                                                            ctcaaaCCGCCACAGCTGCCGCAAACCATTCGGGTTGCTAAAGGTAAATTTACGTAAAGGTTGTTGGCGGGGGCCAATGAACCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                         <  1:1722620/147‑1 (MQ=255)
                                                                                          tCTGGCGCAATCCATTCGCTTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                           <  2:211305/150‑1 (MQ=255)
                                                                                          tCTGGCGCAATCCATTCGCGTGGCGAAAGGTTATTTTACGTTAATGTTGGGTGCGGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                           <  2:1944382/150‑1 (MQ=255)
                                                                                           cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACCCCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATTCCTTATCCAGCATAGCTAATTACGCt                                                          >  1:2007640/1‑150 (MQ=255)
                                                                                            tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                         <  1:548059/150‑1 (MQ=255)
                                                                                            tGGCGCAATCCATGCGCGTTGCTAAACGTTAATTTACGTTAATGTTGGGGGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                         <  2:1305281/150‑1 (MQ=255)
                                                                                             ggCGCAATCCATACGCCTTGCGAAACGTTAATTTACGTTAATGTTGTGAGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTATCCTATTGGTGCCGGATATGCCTTATCCACCATAGCTAAATCAGCTga                                                        >  1:335839/1‑150 (MQ=255)
                                                                                               cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                      >  2:1160887/1‑150 (MQ=255)
                                                                                                 cAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                    <  2:1446043/150‑1 (MQ=255)
                                                                                                     ccATTCGCGTGGCGAAAGGTTAATTTACGTTAATGTTGTGGGCCGGGTGCATTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                <  2:1867782/150‑1 (MQ=255)
                                                                                                       aTTCGCGTTGCGGAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                              <  2:501332/150‑1 (MQ=255)
                                                                                                       aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCCGGTGCAAAGCAAACGGCACACAACATAACACCATATCCAGCGCAGTTTTTCCTTTTTGTGCCGGATATGCCTTATCCACCATAGCAAATTACGCAGAAGAAAGttac                                              >  2:1698999/1‑148 (MQ=255)
                                                                                                         tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACAAAACACCAAATACAGCGCAGGTTTTACTTTTGGTGCCGGATATTCCTTTTCAACCATAGCGTATTACGCTTAAGAAATTTAGAc                                            >  2:1571833/1‑150 (MQ=255)
                                                                                                          cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                           >  2:235922/1‑150 (MQ=255)
                                                                                                             gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                        <  1:534463/150‑1 (MQ=255)
                                                                                                             gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                        <  2:359433/150‑1 (MQ=255)
                                                                                                                gctaaaCTTTATTTTATTTAATTGTTGTGGGCGGGGTCAATTGAACCGGCAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                     <  1:1688221/147‑1 (MQ=255)
                                                                                                                gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                     <  1:1171739/150‑1 (MQ=255)
                                                                                                                gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCACACAACATAACACCATATCCAGCGGAGTTTTTACTTTTGGTGCCGGATATGGCTTATCCAGCATAGCTAATTCCGCTGTAGAAAGTTCGACCACAAGc                                     >  2:410431/1‑150 (MQ=255)
                                                                                                                 cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGAAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                    >  2:1848011/1‑150 (MQ=255)
                                                                                                                  gAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                   <  1:1415995/150‑1 (MQ=255)
                                                                                                                      cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                               >  2:1103558/1‑150 (MQ=255)
                                                                                                                      cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTAGACCTCAAGCACAGcc                               >  1:1656070/1‑150 (MQ=255)
                                                                                                                      cGTTAATTAACGTAAATGTTGTGTGCCGGGTTCAATGCATCCGGAACACAACATCACCCCATCTCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                               <  2:335724/150‑1 (MQ=255)
                                                                                                                       gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                              >  1:35433/1‑150 (MQ=255)
                                                                                                                            tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                         <  1:372826/150‑1 (MQ=255)
                                                                                                                              tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                       <  1:1362412/150‑1 (MQ=255)
                                                                                                                                cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                     <  1:1226910/150‑1 (MQ=255)
                                                                                                                                 gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                    <  2:977771/150‑1 (MQ=255)
                                                                                                                                  ttATTGTTGTGTGCCGGGTGAAATGCATCGGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                   <  2:935750/150‑1 (MQ=255)
                                                                                                                                  ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                   <  2:1210274/150‑1 (MQ=255)
                                                                                                                                   tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATGCCGCTGAAGAAAGTTCGCCCTCACGCACAGCCGCATTTTGTTggc                  >  1:1337555/1‑149 (MQ=255)
                                                                                                                                       gttgtGTGCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGTTATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg              >  2:823736/1‑150 (MQ=255)
                                                                                                                                         tgtttgCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCATTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGATAGTTCGACCTCAAACACAGCCGCATTTTGTTGGACATggg            <  1:1869216/150‑1 (MQ=255)
                                                                                                                                              gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       >  2:480646/1‑150 (MQ=255)
                                                                                                                                              gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       >  1:1639277/1‑150 (MQ=255)
                                                                                                                                                   gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTATTTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  >  1:372165/1‑150 (MQ=255)
                                                                                                                                                   gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  >  1:964693/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
TATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864611‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: