Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 5.6% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 5.6% 311.7 / 3.3 112intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (52/52);  new base T (2/6);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864614‑1864910
                                                                                                                                                     |                                                                                                                                                     
gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTGCGCTTCAGAAACGCAAAAAGTGACCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                       >  1:45450/1‑150 (MQ=255)
gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTGCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                       >  1:1483304/1‑150 (MQ=255)
gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                       >  2:33185/1‑150 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                     <  2:1638633/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                     <  2:1321361/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                     <  2:284087/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                     <  1:1497837/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGAGAAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                     <  1:1028478/150‑1 (MQ=255)
      tgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAta                                                                                                                                                 >  1:1735393/1‑149 (MQ=255)
        atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                               >  2:534463/1‑150 (MQ=255)
         tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                              <  1:505649/150‑1 (MQ=255)
         tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                              <  2:1113142/150‑1 (MQ=255)
           gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATACGGca                                                                                                                                           >  1:1503447/1‑150 (MQ=255)
            cTACAAAACATTTGTTTGTTTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGGAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                          <  1:2492/150‑1 (MQ=255)
            cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                          <  1:1403703/150‑1 (MQ=255)
            cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                          <  1:1840126/150‑1 (MQ=255)
             tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacc                                                                                                                                         >  2:53061/1‑149 (MQ=255)
              aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATACATCCGGcacac                                                                                                                                       <  2:1355610/150‑1 (MQ=255)
              aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                       <  1:550108/150‑1 (MQ=255)
              aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTAGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacac                                                                                                                                       <  2:1553946/150‑1 (MQ=255)
                 aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTTACCAAAGATAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTTAATGTTGGGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                    <  1:865859/150‑1 (MQ=255)
                  aaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACTCAAAAACTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaaca                                                                                                                                   >  2:361577/1‑150 (MQ=255)
                    cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAta                                                                                                                                 >  1:82/1‑149 (MQ=255)
                     aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGATCAATCCATTCGAGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCACGGTTCAATCCATCCGGCACACAACATca                                                                                                                                >  1:130692/1‑150 (MQ=255)
                        tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                             >  1:1172381/1‑150 (MQ=255)
                        tgtatgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATACGCGTTGCGAAACGCTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAttaacg                                                                                                                             <  1:341806/150‑6 (MQ=38)
                         gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                            <  1:1213815/150‑1 (MQ=255)
                         gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGAGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCTATGCATCCGGCACACAACATCACAcc                                                                                                                            <  2:322886/150‑1 (MQ=255)
                            tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                         >  2:1212581/1‑150 (MQ=255)
                              tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc                                                                                                                       >  2:711291/1‑150 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATACa                                                                                                                     >  1:1938599/1‑150 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCAGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCa                                                                                                                     >  2:224509/1‑150 (MQ=255)
                                  gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                   >  2:1415994/1‑150 (MQ=255)
                                    aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAgcgc                                                                                                                 >  1:1090724/1‑150 (MQ=255)
                                    aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATACAgcgc                                                                                                                 >  2:1688220/1‑150 (MQ=255)
                                            aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCGATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGGTTTTc                                                                                                         <  2:854321/150‑1 (MQ=255)
                                             cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                        <  2:1910962/150‑1 (MQ=255)
                                             cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAAAGCATCCCGCACACAACATCACACCATATCCACCGCAGTTTTTcc                                                                                                        >  1:1944383/1‑150 (MQ=255)
                                              gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                       >  2:1050825/1‑150 (MQ=255)
                                               cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                      <  2:1814357/150‑1 (MQ=255)
                                               cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                      >  2:29393/1‑150 (MQ=255)
                                               cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAAACGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCtt                                                                                                      >  1:1930348/1‑150 (MQ=255)
                                                  cataaaCCCAAAAAGTCACCAAAGAGAAACCTGCACATCGGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTTAATGTTGTGGGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   <  2:1297874/147‑1 (MQ=255)
                                                  cAGAAGCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   >  2:1432404/1‑150 (MQ=255)
                                                  cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   >  2:1925857/1‑150 (MQ=255)
                                                    gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                 >  2:1392083/1‑150 (MQ=255)
                                                       aCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGcc                                                                                              <  1:891864/150‑1 (MQ=255)
                                                        cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                             <  2:411148/150‑1 (MQ=255)
                                                         gCAAAAAGTGACCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                            <  2:1668521/150‑1 (MQ=255)
                                                          cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTTCAAAACATACGGCACACCACATAACACCATATCCAGCGGAGTGTTTCCTTTTGGTGACgct                                                                                           >  2:1525400/1‑148 (MQ=255)
                                                          cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCCATGCATACGGCACACCACATCACCCCATTTCCCGCGGAGGTTTTGCTGTTGGTGCCggg                                                                                           >  2:1981743/1‑149 (MQ=255)
                                                          cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                           <  1:596015/150‑1 (MQ=255)
                                                            aaaaGTGACCAAAGAGAAACCTGAACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACAACACCCCAAATCCAGCGACGTTTTTAATTTTGGTTCCGGata                                                                                         >  2:1780142/1‑150 (MQ=255)
                                                                gTGACCATAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATTTTGTGTGCCGGTTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                     <  1:1315087/150‑1 (MQ=255)
                                                                  gACCAAAGCGACACCTGAACATCTGTCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                   <  2:1260427/150‑1 (MQ=255)
                                                                   aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                  <  2:1210886/150‑1 (MQ=255)
                                                                    ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTAACGTTAATGTTGTGTGCCGGGTGCCAGGCAACCCGCCCCCCCCAACCCGCCAATTCCCGCGGAGTTTTAGCGTTTGGTGCCGGATTTGCCGTAt                                                                                 >  1:1007307/1‑150 (MQ=255)
                                                                    ccAAAGAGAAACATGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTTCAATGAATCAGGGACAACAACTCACACCATATGACTAGCAGTTTTTGCTTTTGGTGACCGATATGCCatat                                                                                 >  1:698447/1‑150 (MQ=255)
                                                                     cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                >  2:1611486/1‑150 (MQ=255)
                                                                      aaaGACAAACCTCCACATCTGGCGCAATCCATTCGCGTGGCTAAACTTAAATTACCTTTAATGTGGTGTGCCGGGGGAATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                               <  2:1525084/150‑1 (MQ=255)
                                                                        agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                             <  2:1127654/150‑1 (MQ=255)
                                                                               ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                      <  1:650966/150‑1 (MQ=255)
                                                                                       tCTGGCGCAATCCATTCGCGTGGCGAAAGGTTATTTTACGTTAATGTTGGGTGCGGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                              <  2:1944382/150‑1 (MQ=255)
                                                                                         tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                            <  1:548059/150‑1 (MQ=255)
                                                                                         tGGCGCAATCCATGCGCGTTGCTAAACGTTAATTTACGTTAATGTTGGGGGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                            <  2:1305281/150‑1 (MQ=255)
                                                                                          ggCGCAATCCATACGCCTTGCGAAACGTTAATTTACGTTAATGTTGTGAGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTATCCTATTGGTGCCGGATATGCCTTATCCACCATAGCTAAATCAGCTga                                                           >  1:335839/1‑150 (MQ=255)
                                                                                            cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                         >  2:1160887/1‑150 (MQ=255)
                                                                                              cAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                       <  2:1446043/150‑1 (MQ=255)
                                                                                                  ccATTCGCGTGGCGAAAGGTTAATTTACGTTAATGTTGTGGGCCGGGTGCATTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                   <  2:1867782/150‑1 (MQ=255)
                                                                                                    aTTCGCGTTGCGGAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                 <  2:501332/150‑1 (MQ=255)
                                                                                                    aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCCGGTGCAAAGCAAACGGCACACAACATAACACCATATCCAGCGCAGTTTTTCCTTTTTGTGCCGGATATGCCTTATCCACCATAGCAAATTACGCAGAAGAAAGttac                                                 >  2:1698999/1‑148 (MQ=255)
                                                                                                       cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                              >  2:235922/1‑150 (MQ=255)
                                                                                                          gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                           <  1:534463/150‑1 (MQ=255)
                                                                                                             gctaaaCTTTATTTTATTTAATTGTTGTGGGCGGGGTCAATTGAACCGGCAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                        <  1:1688221/147‑1 (MQ=255)
                                                                                                             gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                        <  1:1171739/150‑1 (MQ=255)
                                                                                                             gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCACACAACATAACACCATATCCAGCGGAGTTTTTACTTTTGGTGCCGGATATGGCTTATCCAGCATAGCTAATTCCGCTGTAGAAAGTTCGACCACAAGc                                        >  2:410431/1‑150 (MQ=255)
                                                                                                              cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGAAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                       >  2:1848011/1‑150 (MQ=255)
                                                                                                               gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                      <  1:1408441/150‑1 (MQ=255)
                                                                                                                   cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTAGACCTCAAGCACAGcc                                  >  1:1656070/1‑150 (MQ=255)
                                                                                                                   cGTTAATTAACGTAAATGTTGTGTGCCGGGTTCAATGCATCCGGAACACAACATCACCCCATCTCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                  <  2:335724/150‑1 (MQ=255)
                                                                                                                    gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                 >  1:35433/1‑150 (MQ=255)
                                                                                                                         tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                            <  1:372826/150‑1 (MQ=255)
                                                                                                                           tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                          <  1:1362412/150‑1 (MQ=255)
                                                                                                                             cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                        <  1:1226910/150‑1 (MQ=255)
                                                                                                                              gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                       <  2:977771/150‑1 (MQ=255)
                                                                                                                               ttATTGTTGTGTGCCGGGTGAAATGCATCGGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                      <  2:935750/150‑1 (MQ=255)
                                                                                                                               ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                      <  2:1210274/150‑1 (MQ=255)
                                                                                                                               ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                      <  1:939777/150‑1 (MQ=255)
                                                                                                                                tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATGCCGCTGAAGAAAGTTCGCCCTCACGCACAGCCGCATTTTGTTggc                     >  1:1337555/1‑149 (MQ=255)
                                                                                                                                  ttgttgtGTTCCGGTGGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACa                   <  2:1567809/149‑1 (MQ=255)
                                                                                                                                   tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                  >  2:1160751/1‑150 (MQ=255)
                                                                                                                                    gttgtGTGCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGTTATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg                 >  2:823736/1‑150 (MQ=255)
                                                                                                                                      tgtttgCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCATTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGATAGTTCGACCTCAAACACAGCCGCATTTTGTTGGACATggg               <  1:1869216/150‑1 (MQ=255)
                                                                                                                                           gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc          >  2:480646/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc          >  1:1639277/1‑150 (MQ=255)
                                                                                                                                                gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTATTTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc     >  1:372165/1‑150 (MQ=255)
                                                                                                                                                gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc     >  1:964693/1‑150 (MQ=255)
                                                                                                                                                  gCAATGCATCCGGCACACAACATCACACCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg   >  1:1860121/1‑150 (MQ=255)
                                                                                                                                                   cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  2:708266/1‑150 (MQ=255)
                                                                                                                                                   cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGACGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  1:1091585/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864614‑1864910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: