Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 6.7% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 6.7% 286.9 / 8.2 110intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (48/49);  new base C (2/5);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 113 total aligned reads displayed.

TGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864620‑1864917
                                                                                                                                                    |                                                                                                                                                       
tgatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAta                                                                                                                                                        >  1:1735393/1‑149 (MQ=255)
   tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  1:505649/150‑1 (MQ=255)
   tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                     <  2:1113142/150‑1 (MQ=255)
     gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATACGGca                                                                                                                                                  >  1:1503447/1‑150 (MQ=255)
      cTACAAAACATTTGTTTGTTTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGGAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                                 <  1:2492/150‑1 (MQ=255)
      cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                                 <  1:1403703/150‑1 (MQ=255)
      cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                                 <  1:1840126/150‑1 (MQ=255)
       tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacc                                                                                                                                                >  2:53061/1‑149 (MQ=255)
        aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATACATCCGGcacac                                                                                                                                              <  2:1355610/150‑1 (MQ=255)
        aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                              <  1:550108/150‑1 (MQ=255)
        aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTAGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacac                                                                                                                                              <  2:1553946/150‑1 (MQ=255)
           aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTTACCAAAGATAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTTAATGTTGGGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                           <  1:865859/150‑1 (MQ=255)
            aaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACTCAAAAACTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaaca                                                                                                                                          >  2:361577/1‑150 (MQ=255)
              cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAta                                                                                                                                        >  1:82/1‑149 (MQ=255)
               aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCACATCTGGATCAATCCATTCGAGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCACGGTTCAATCCATCCGGCACACAACATca                                                                                                                                       >  1:130692/1‑150 (MQ=255)
                  tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                                    >  1:1172381/1‑150 (MQ=255)
                  tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                                    >  2:650966/1‑150 (MQ=255)
                  tgtatgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATACGCGTTGCGAAACGCTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAttaacg                                                                                                                                    <  1:341806/150‑6 (MQ=38)
                   gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAcc                                                                                                                                   <  1:1213815/150‑1 (MQ=255)
                   gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGAGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCTATGCATCCGGCACACAACATCACAcc                                                                                                                                   <  2:322886/150‑1 (MQ=255)
                        tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc                                                                                                                              >  2:711291/1‑150 (MQ=255)
                          tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATCACACCATATACa                                                                                                                            >  1:1938599/1‑150 (MQ=255)
                          tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCAGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCa                                                                                                                            >  2:224509/1‑150 (MQ=255)
                            gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                          >  2:1415994/1‑150 (MQ=255)
                            gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                          >  2:1408440/1‑150 (MQ=255)
                              aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAgcgc                                                                                                                        >  1:1090724/1‑150 (MQ=255)
                                      aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCGATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGGTTTTc                                                                                                                <  2:854321/150‑1 (MQ=255)
                                       cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                               <  2:1910962/150‑1 (MQ=255)
                                       cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTcc                                                                                                               >  1:475667/1‑150 (MQ=255)
                                       cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAAAGCATCCCGCACACAACATCACACCATATCCACCGCAGTTTTTcc                                                                                                               >  1:1944383/1‑150 (MQ=255)
                                        gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                              >  2:1050825/1‑150 (MQ=255)
                                         cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                             >  2:29393/1‑150 (MQ=255)
                                         cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                             <  2:1814357/150‑1 (MQ=255)
                                            cataaaCCCAAAAAGTCACCAAAGAGAAACCTGCACATCGGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTTAATGTTGTGGGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          <  2:1297874/147‑1 (MQ=255)
                                            cAGAAGCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          >  2:1432404/1‑150 (MQ=255)
                                            cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                          >  2:1925857/1‑150 (MQ=255)
                                              gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                        >  2:1392083/1‑150 (MQ=255)
                                               aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                       <  1:1215546/150‑1 (MQ=255)
                                                 aCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGcc                                                                                                     <  1:891864/150‑1 (MQ=255)
                                                  cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                    <  2:411148/150‑1 (MQ=255)
                                                   gCAAAAAGTGACCAATGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                                   <  2:1668521/150‑1 (MQ=255)
                                                    cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTTCAAAACATACGGCACACCACATAACACCATATCCAGCGGAGTGTTTCCTTTTGGTGACgct                                                                                                  >  2:1525400/1‑148 (MQ=255)
                                                    cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCCATGCATACGGCACACCACATCACCCCATTTCCCGCGGAGGTTTTGCTGTTGGTGCCggg                                                                                                  >  2:1981743/1‑149 (MQ=255)
                                                    cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGa                                                                                                  <  1:596015/150‑1 (MQ=255)
                                                      aaaaGTGACCAAAGAGAAACCTGAACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACAACACCCCAAATCCAGCGACGTTTTTAATTTTGGTTCCGGata                                                                                                >  2:1780142/1‑150 (MQ=255)
                                                          gTGACCATAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATTTTGTGTGCCGGTTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                            <  1:1315087/150‑1 (MQ=255)
                                                            gACCAAAGCGACACCTGAACATCTGTCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                          <  2:1260427/150‑1 (MQ=255)
                                                             aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                         <  2:1210886/150‑1 (MQ=255)
                                                              ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTAACGTTAATGTTGTGTGCCGGGTGCCAGGCAACCCGCCCCCCCCAACCCGCCAATTCCCGCGGAGTTTTAGCGTTTGGTGCCGGATTTGCCGTAt                                                                                        >  1:1007307/1‑150 (MQ=255)
                                                              ccAAAGAGAAACATGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTTCAATGAATCAGGGACAACAACTCACACCATATGACTAGCAGTTTTTGCTTTTGGTGACCGATATGCCatat                                                                                        >  1:698447/1‑150 (MQ=255)
                                                               cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                       >  2:1611486/1‑150 (MQ=255)
                                                                aaaGACAAACCTCCACATCTGGCGCAATCCATTCGCGTGGCTAAACTTAAATTACCTTTAATGTGGTGTGCCGGGGGAATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                                      <  2:1525084/150‑1 (MQ=255)
                                                                 aaGAGAAACCTGCACATCTTGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                     <  1:66472/150‑1 (MQ=255)
                                                                  agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                                    <  2:1127654/150‑1 (MQ=255)
                                                                   ctcaaaCCGCCACAGCTGCCGCAAACCATTCGGGTTGCTAAAGGTAAATTTACGTAAAGGTTGTTGGCGGGGGCCAATGAACCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                                   <  1:1722620/147‑1 (MQ=255)
                                                                                 tCTGGCGCAATCCATTCGCTTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                                     <  2:211305/150‑1 (MQ=255)
                                                                                  cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACCCCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATTCCTTATCCAGCATAGCTAATTACGCt                                                                    >  1:2007640/1‑150 (MQ=255)
                                                                                   tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                                   <  1:548059/150‑1 (MQ=255)
                                                                                   tGGCGCAATCCATGCGCGTTGCTAAACGTTAATTTACGTTAATGTTGGGGGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                                   <  2:1305281/150‑1 (MQ=255)
                                                                                    ggCGCAATCCATACGCCTTGCGAAACGTTAATTTACGTTAATGTTGTGAGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTATCCTATTGGTGCCGGATATGCCTTATCCACCATAGCTAAATCAGCTga                                                                  >  1:335839/1‑150 (MQ=255)
                                                                                      cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                                >  2:1160887/1‑150 (MQ=255)
                                                                                            ccATTCGCGTGGCGAAAGGTTAATTTACGTTAATGTTGTGGGCCGGGTGCATTGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                          <  2:1867782/150‑1 (MQ=255)
                                                                                              aTTCGCGTTGCGGAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                        <  2:501332/150‑1 (MQ=255)
                                                                                              aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCCGGTGCAAAGCAAACGGCACACAACATAACACCATATCCAGCGCAGTTTTTCCTTTTTGTGCCGGATATGCCTTATCCACCATAGCAAATTACGCAGAAGAAAGttac                                                        >  2:1698999/1‑148 (MQ=255)
                                                                                                tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACAAAACACCAAATACAGCGCAGGTTTTACTTTTGGTGCCGGATATTCCTTTTCAACCATAGCGTATTACGCTTAAGAAATTTAGAc                                                      >  2:1571833/1‑150 (MQ=255)
                                                                                                 cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                                     >  2:235922/1‑150 (MQ=255)
                                                                                                    gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                  <  1:534463/150‑1 (MQ=255)
                                                                                                       gctaaaCTTTATTTTATTTAATTGTTGTGGGCGGGGTCAATTGAACCGGCAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                               <  1:1688221/147‑1 (MQ=255)
                                                                                                       gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                               <  1:1171739/150‑1 (MQ=255)
                                                                                                       gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCACACAACATAACACCATATCCAGCGGAGTTTTTACTTTTGGTGCCGGATATGGCTTATCCAGCATAGCTAATTCCGCTGTAGAAAGTTCGACCACAAGc                                               >  2:410431/1‑150 (MQ=255)
                                                                                                        cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGAAGTTTTTCATTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                              >  2:1848011/1‑150 (MQ=255)
                                                                                                         gAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                             <  1:1415995/150‑1 (MQ=255)
                                                                                                         gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                             <  1:1408441/150‑1 (MQ=255)
                                                                                                             cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTAGACCTCAAGCACAGcc                                         >  1:1656070/1‑150 (MQ=255)
                                                                                                             cGTTAATTAACGTAAATGTTGTGTGCCGGGTTCAATGCATCCGGAACACAACATCACCCCATCTCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                         <  2:335724/150‑1 (MQ=255)
                                                                                                              gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                        >  1:35433/1‑150 (MQ=255)
                                                                                                                   tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                                   <  1:372826/150‑1 (MQ=255)
                                                                                                                     tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                                 <  1:1362412/150‑1 (MQ=255)
                                                                                                                        gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                              <  2:977771/150‑1 (MQ=255)
                                                                                                                         ttATTGTTGTGTGCCGGGTGAAATGCATCGGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                             <  2:935750/150‑1 (MQ=255)
                                                                                                                         ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                             <  1:939777/150‑1 (MQ=255)
                                                                                                                         ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                             <  2:1210274/150‑1 (MQ=255)
                                                                                                                          tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATGCCGCTGAAGAAAGTTCGCCCTCACGCACAGCCGCATTTTGTTggc                            >  1:1337555/1‑149 (MQ=255)
                                                                                                                            ttgttgtGTTCCGGTGGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACa                          <  2:1567809/149‑1 (MQ=255)
                                                                                                                             tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                         >  2:1160751/1‑150 (MQ=255)
                                                                                                                              gttgtGTGCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGTTATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATg                        >  2:823736/1‑150 (MQ=255)
                                                                                                                                tgtttgCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCATTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGATAGTTCGACCTCAAACACAGCCGCATTTTGTTGGACATggg                      <  1:1869216/150‑1 (MQ=255)
                                                                                                                                     gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc                 >  2:480646/1‑150 (MQ=255)
                                                                                                                                     gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc                 >  1:1639277/1‑150 (MQ=255)
                                                                                                                                          gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc            >  1:964693/1‑150 (MQ=255)
                                                                                                                                            gCAATGCATCCGGCACACAACATCACACCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg          >  1:1860121/1‑150 (MQ=255)
                                                                                                                                             cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc         >  2:708266/1‑150 (MQ=255)
                                                                                                                                             cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGACGCATTTTGTTGGACATGGGCAATCGTGGCCGc         >  1:1091585/1‑150 (MQ=255)
                                                                                                                                                tGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCttt      >  2:293573/1‑150 (MQ=255)
                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg    <  2:389371/150‑1 (MQ=255)
                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAGCGTGGCCGCTTTTg    <  2:45450/150‑1 (MQ=255)
                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  1:51735/150‑1 (MQ=255)
                                                                                                                                                  aaTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg    <  2:1591123/149‑1 (MQ=255)
                                                                                                                                                   aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   <  2:1172380/150‑1 (MQ=255)
                                                                                                                                                    tCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTAATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGa  <  1:1053913/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864620‑1864917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: