Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,896,468 T→G 5.0% V100G (GTG→GGG)  nudL → putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,896,4680TG 5.0% 336.1 / 5.7 111V100G (GTG→GGG) nudLputative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (53/51);  new base G (2/4);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 120 total aligned reads displayed.

GCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAT  >  NC_000913/1896319‑1896616
                                                                                                                                                     |                                                                                                                                                    
gCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGt                                                                                                                                                      >  2:910042/1‑150 (MQ=255)
 cGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtg                                                                                                                                                     <  1:1687416/150‑1 (MQ=255)
 cGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtg                                                                                                                                                     <  1:532937/150‑1 (MQ=255)
 cGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtg                                                                                                                                                     >  1:80934/1‑150 (MQ=255)
 cGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTGGAAGTTATCGGCGtg                                                                                                                                                     >  1:1252548/1‑150 (MQ=255)
  gTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtgc                                                                                                                                                    <  2:569112/150‑1 (MQ=255)
   ttCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtgct                                                                                                                                                   >  2:498282/1‑150 (MQ=255)
   ttCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtgct                                                                                                                                                   >  1:624559/1‑150 (MQ=255)
     cGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtgctgc                                                                                                                                                 >  2:1792067/1‑150 (MQ=255)
      gATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTgcc                                                                                                                                                >  2:932820/1‑150 (MQ=255)
          cATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGTGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                                                                                                            >  1:1834363/1‑150 (MQ=255)
          cATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                                                                                                            >  2:1196419/1‑150 (MQ=255)
           aTCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCg                                                                                                                                           <  1:1137370/150‑1 (MQ=255)
             cTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                         <  1:1893846/150‑1 (MQ=255)
             cTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGGCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                                                                                                                         <  1:1795002/150‑1 (MQ=255)
              tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTTCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCg                                                                                                                                        <  1:1935923/150‑1 (MQ=255)
              tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCCGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCg                                                                                                                                        >  1:1119133/1‑150 (MQ=255)
              tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCg                                                                                                                                        <  2:1854945/150‑1 (MQ=255)
              tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCg                                                                                                                                        <  2:1713898/150‑1 (MQ=255)
                cGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGaa                                                                                                                                      >  2:199344/1‑149 (MQ=255)
                cGTAAACACGCTGGACAAGTGGCATTCCATGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTTTACCGCATTCCGCCGTGGAAGTTATCGGCGTGCTGCCGCCAGTCgtt                                                                                                                                      >  2:338192/1‑148 (MQ=255)
                  tAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAg                                                                                                                                    <  1:1038724/150‑1 (MQ=255)
                    aaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCg                                                                                                                                  >  1:1288698/1‑150 (MQ=255)
                    aaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCg                                                                                                                                  <  1:726000/150‑1 (MQ=255)
                          cTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTg                                                                                                                            <  1:1791101/150‑1 (MQ=255)
                           tGGACAAGTGGCATTCCCTGGAGTTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCCCTgg                                                                                                                           >  2:701100/1‑150 (MQ=255)
                               cAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAc                                                                                                                       >  2:1409872/1‑150 (MQ=255)
                               cAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAc                                                                                                                       >  2:1675528/1‑150 (MQ=255)
                               cAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAc                                                                                                                       <  1:1068309/150‑1 (MQ=255)
                               cAAGTGGCATTCCCTGGAGGTGCAGGCGAGGACCCGGACGCATCAGCGAGCGCCGCCGCGCTGCGCGCAGCTGAAGCAGAGGGCGCTATACCGCCTGCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAc                                                                                                                       <  1:291352/150‑1 (MQ=255)
                                 aGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCa                                                                                                                     >  2:1251870/1‑150 (MQ=255)
                                  gTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAg                                                                                                                    <  1:509931/150‑1 (MQ=255)
                                  gTGGCATTCCCTGGAGGTGCAGTCGAGGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAg                                                                                                                    <  1:867341/150‑1 (MQ=255)
                                      cATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCAGCCGTTGAAGTTATCGGCGTGCTGGCGCCCGTCGATAGCGTCACTGGCGAGCAGGTag                                                                                                                >  2:2048621/1‑149 (MQ=255)
                                          cccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAcccc                                                                                                            <  1:2043540/150‑1 (MQ=255)
                                            cTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAg                                                                                                          >  2:1081722/1‑150 (MQ=255)
                                             tGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGGTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGt                                                                                                         <  1:396056/150‑1 (MQ=255)
                                              ggAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCCCTGGCTACCAGGTACCCCCAGTg                                                                                                        >  2:401613/1‑150 (MQ=255)
                                               gAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTgg                                                                                                       <  1:1711531/150‑1 (MQ=255)
                                                    gCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGc                                                                                                  <  2:1142813/150‑1 (MQ=255)
                                                      aGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTACCCCCAGTGGTCGGCAt                                                                                                >  2:946825/1‑150 (MQ=255)
                                                         cGATGACACGGACGCATCAGCTATCGACTCCGCGCTGCGAGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGAGGTCGGCATTAt                                                                                             >  1:681270/1‑150 (MQ=255)
                                                          gATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACAGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCTTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGCTCGGCATTATc                                                                                            >  2:1015513/1‑150 (MQ=255)
                                                                ccGGACGCATCAGCTCTCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGGCGCTATACCGCCTTCCGCCGTGGAAGTTAACGGCGTGCAGCCGCCCGTCGATAGCCTCACTGGCTACCAGGTAACCCCAGTGGGCGGCATAATcccgccc                                                                                      <  2:330882/149‑1 (MQ=255)
                                                                acGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATcccgccc                                                                                      <  2:928410/150‑1 (MQ=255)
                                                                  ggACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCCGTGGTCGGCATTATCCCGCCCGa                                                                                    >  2:1534790/1‑150 (MQ=255)
                                                                  ggACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGa                                                                                    <  1:910043/150‑1 (MQ=255)
                                                                   caCGCATCAGCTATCGCCGCCGCGCAGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGAt                                                                                   >  2:1106445/2‑150 (MQ=255)
                                                                       cATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGc                                                                               >  1:610116/1‑150 (MQ=255)
                                                                         ccAGCCATCGCCGCCGCGCGGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTACCGCCGTAGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCg                                                                             <  1:338192/149‑1 (MQ=255)
                                                                           aGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTa                                                                           >  2:474194/1‑150 (MQ=255)
                                                                           aGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTa                                                                           <  2:1514829/150‑1 (MQ=255)
                                                                            gCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAGCCCCAGTGGTCGGCAGTATCCCGCCCGATCTGCCGTAt                                                                          >  1:1801356/1‑150 (MQ=255)
                                                                               aTCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATcgc                                                                       >  1:1255790/1‑150 (MQ=255)
                                                                                 cgccgccgtGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCgcgc                                                                     >  2:1644826/1‑150 (MQ=255)
                                                                                  gccgccgcGCTGCGCGAAGCTGAAGAAGAGGTCGCTCTACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGcc                                                                    <  1:326201/150‑1 (MQ=255)
                                                                                         cgcTGCGCGAAGCTGAAGAAGAGGTCGCTATACCTCCTTCCGCCGTTGAAGTTATCGGAGTTCTGCCGCCCGTCGATAGCGTCACTGGCTACCATGTAAACCCATTGGTCGGCATTATCCCGTCCGATCTGCCGTATCGCGCCAGTGAAg                                                             <  2:277017/150‑1 (MQ=255)
                                                                                         cgcTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAg                                                             >  1:1764700/1‑150 (MQ=255)
                                                                                         cgcTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAg                                                             <  2:755636/150‑1 (MQ=255)
                                                                                          gcTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGa                                                            >  2:606059/1‑150 (MQ=255)
                                                                                          gcTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGa                                                            >  2:1436229/1‑150 (MQ=255)
                                                                                             gcgcGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGCAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTATCCCCAGTGGTCGGCATTATCCCGCCCTATCTGCCGTCTCGGGCCAGGGAAGATgg                                                         >  1:321340/1‑149 (MQ=255)
                                                                                              cgcgAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGaa                                                        >  1:17411/1‑150 (MQ=255)
                                                                                               gcgAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAg                                                       >  1:1079331/1‑150 (MQ=255)
                                                                                               gcgAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAg                                                       >  1:688530/1‑150 (MQ=255)
                                                                                                   aGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGtctc                                                   <  2:1993207/150‑1 (MQ=255)
                                                                                                   aGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGtctc                                                   <  2:1985501/150‑1 (MQ=255)
                                                                                                     cTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcgg                                                 <  2:624559/150‑1 (MQ=255)
                                                                                                     cTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGGATCGCGCCAGTGAAGAGGAAGTCTcgg                                                 >  2:207144/1‑150 (MQ=255)
                                                                                                     cTGAAGAAGAGGTCGCTATACAGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCACGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcgg                                                 <  1:60310/150‑1 (MQ=255)
                                                                                                      tGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcggc                                                >  2:266801/1‑150 (MQ=255)
                                                                                                      tGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGAGCAGCCGCCCGTCGATAGCGTCACTGGCTACCAGGAAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGTTGAAGTCTcggc                                                >  1:223555/1‑150 (MQ=255)
                                                                                                      ggaagaaGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcggc                                                <  1:2013211/149‑1 (MQ=255)
                                                                                                          gaagaGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGgtg                                            <  1:1115472/150‑1 (MQ=255)
                                                                                                          gaagaGGGCGCTATACCGCCTAGCGCCGTTGAAGTTTTCTGCGTGCTTCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGGCGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGgtg                                            <  2:196109/150‑1 (MQ=255)
                                                                                                           aagaGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGgtgt                                           <  1:1490552/150‑1 (MQ=255)
                                                                                                            agagGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGtt                                          >  1:1122436/1‑150 (MQ=255)
                                                                                                               ggTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGa                                       >  1:2022206/1‑150 (MQ=255)
                                                                                                                  cGCTATACCTCCTTCCGCCGTTGAAGATATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAAt                                    <  2:1046791/150‑1 (MQ=255)
                                                                                                                  cGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGGAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAAt                                    <  1:1294978/150‑1 (MQ=255)
                                                                                                                      ataCCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCg                                <  1:116513/150‑1 (MQ=255)
                                                                                                                      ataCCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCg                                >  1:1643531/1‑150 (MQ=255)
                                                                                                                       taCCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGCAATGCCGc                               >  1:1519446/1‑150 (MQ=255)
                                                                                                                        aCCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGGATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCt                              >  2:1600910/1‑150 (MQ=255)
                                                                                                                         ccGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTTCCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGGGAAGCTGAAGTCTCGGCGGTGTTTGAAATGCCGCTc                             >  1:133865/1‑150 (MQ=255)
                                                                                                                         ccGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTc                             >  1:1410037/1‑150 (MQ=255)
                                                                                                                          cGCCTGCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCg                            <  1:103458/150‑1 (MQ=255)
                                                                                                                              ttCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCa                        >  1:1956054/1‑150 (MQ=255)
                                                                                                                              ttCCGCCGTTGAAGGTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCa                        <  1:1572017/150‑1 (MQ=255)
                                                                                                                               tCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAGCCGAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAATTGCCGCTCGCCCAg                       <  1:1717859/150‑1 (MQ=255)
                                                                                                                                ccgccgTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTTCCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAgg                      >  1:1961667/1‑150 (MQ=255)
                                                                                                                                  gccgTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCa                    <  2:60015/150‑1 (MQ=255)
                                                                                                                                           gctATCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTg           >  2:262181/3‑150 (MQ=255)
                                                                                                                                           gTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCGCGCCCAGGCATTACATCTg           <  1:1081723/150‑1 (MQ=255)
                                                                                                                                             tATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTggg         <  1:1675529/150‑1 (MQ=255)
                                                                                                                                             tATCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcggcg                                               >  1:1217547/1‑112 (MQ=255)
                                                                                                                                             tATCGACGGGCGGCAGCACGCCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTcggcg                                               <  2:1217546/112‑1 (MQ=255)
                                                                                                                                               tCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTc       <  2:1957394/150‑1 (MQ=255)
                                                                                                                                                 ggCGGGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGt     <  1:227647/150‑1 (MQ=255)
                                                                                                                                                    gTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAt  >  1:715320/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTATCCCGCCCGATCTGCCGTATCGCGCCAGTGAAGATGAAGTCTCGGCGGTGTTTGAAATGCCGCTCGCCCAGGCATTACATCTGGGTCGTTAT  >  NC_000913/1896319‑1896616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: