Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 184,253 C→A 8.9% intergenic (‑158/+4) yaeH ← / ← yaeI UPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913184,2530CA 8.9% 199.4 / 3.9 78intergenic (‑158/+4)yaeH/yaeIUPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base C (39/31);  new base A (2/6);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAG  >  NC_000913/184104‑184402
                                                                                                                                                     |                                                                                                                                                     
cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAATATCAAAAAATTAACTGATTTATTCTGGTGATAAAATTCAc                                                                                                                                                       >  1:117410/1‑150 (MQ=255)
cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAc                                                                                                                                                       >  1:1660761/1‑150 (MQ=255)
cTCAAAATGAGTCACTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTCATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAc                                                                                                                                                       <  2:153176/150‑1 (MQ=255)
   aaaaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGAt                                                                                                                                                    <  2:642799/150‑1 (MQ=255)
    aaaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGAta                                                                                                                                                   >  1:874294/1‑149 (MQ=255)
     aaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCt                                                                                                                                                  <  1:770222/150‑1 (MQ=255)
     aaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCt                                                                                                                                                  <  1:1743443/150‑1 (MQ=255)
     aaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGACCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGTCGTAAAAATCAAAAAATTACCTGCTTTATTATGGTGATAAAATTCACGATCt                                                                                                                                                  >  2:37222/1‑150 (MQ=255)
          gTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTCCTTTATTCTGGTGATAAAATTCACGATCTACAcc                                                                                                                                             >  1:1637421/1‑150 (MQ=255)
              gTAAATTGTGCTTATATTAGCATTTGGCCTCGCCCGCCCGGCTCGACTCTGATCGCAGAAAGCCTTTTTCTTCCAGCCTTCTATCATCGCGTAAAAATCAAAAAATAACCTGCTTTATTCTGCTGATAAAATTCAAGATCTACACCACtt                                                                                                                                         >  2:300743/1‑150 (MQ=255)
                 aaTTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGAGCTCGCAGAACGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTCTTTTGTTTAAATTCTAGATCTACACCAGTTCCa                                                                                                                                      <  2:826894/150‑1 (MQ=255)
                  aTTGTGCTTATTATAGCATTTGGACTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCATTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAg                                                                                                                                     <  1:192033/150‑1 (MQ=255)
                      tgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGTTGATAAAATTCACGATCTACACCAGTTACAGCATc                                                                                                                                 >  2:1712694/1‑150 (MQ=255)
                       gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCATTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCg                                                                                                                                <  1:284390/150‑1 (MQ=255)
                          tcatttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCAATCATTGAGTAAAAATCAAAAAATTTTTTTTTTTATTGATTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTa                                                                                                                             <  2:189103/147‑1 (MQ=255)
                            ttttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGCACGCCTTTTTCGTCCAGACTTCTATCATGTCGTAATAATCAAAAAATTACCTGCTTTATTCTGGGGATAAAATTCACGATCGACACCAGTTCCAGCATCGTTAca                                                                                                                           >  2:333412/1‑149 (MQ=255)
                             tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTACCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACtt                                                                                                                          <  1:726884/150‑1 (MQ=255)
                               tAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTcc                                                                                                                        >  2:63962/1‑150 (MQ=255)
                                     ttGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                                  <  1:1545643/150‑1 (MQ=255)
                                      tggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGCTCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                                 >  1:260270/1‑150 (MQ=255)
                                      tggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCATCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                                 <  1:612334/150‑1 (MQ=255)
                                        gcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGTGCGGCAg                                                                                                               >  1:1867509/1‑150 (MQ=255)
                                           tggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                                            <  1:763149/150‑1 (MQ=255)
                                              cccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTAATTCCGGGCGGCAGTTCAGa                                                                                                         >  1:1288586/1‑150 (MQ=255)
                                               ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                                        >  2:764290/1‑150 (MQ=255)
                                               ccgcccgGCTCGACTGTGATCGCAGAAAGCCGTTTTCATCCAGACTTCTCTCATGGCGTAAAAAGCAAAAAATTACCTGCTTTATTCTGCTGATAAAATTCACGAGCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                                        <  1:323546/150‑1 (MQ=255)
                                                cgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCATCCAGACTTCTATCATGGCGAAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATACACGATCTACACCAGTTCCAGCATCGATACTTCCGAGCAGCAGTTCAGAcc                                                                                                       >  2:1557836/1‑149 (MQ=255)
                                                  cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAATATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                                     <  1:1654263/150‑1 (MQ=255)
                                                      gCTCGCCTGTGATCGCAGAAAGCCTGTTTCTTCCAGACTTCTATCATGGCGTAAAACTCAAAAAATTACCTGCTTTATTCTGGTGATAAAAATCACGATCTACACCAGTTCCAGCAGCGTTACTTCCGGGCGGCAGTTCAGACGCAAAcc                                                                                                 <  1:1859607/150‑1 (MQ=255)
                                                       cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                                >  2:1953885/1‑150 (MQ=255)
                                                          gACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATTGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATAc                                                                                             <  1:1170057/150‑1 (MQ=255)
                                                            cTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGCTCTACACCAGTTCCAGCATCGTTACTACCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                           >  2:395146/1‑150 (MQ=255)
                                                              gtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGTTGATAAAATTCACGATCTAAACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATAAAAAc                                                                                         >  1:1466383/1‑150 (MQ=255)
                                                              gtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAAc                                                                                         >  2:450869/1‑150 (MQ=255)
                                                                gATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTg                                                                                       >  2:1317047/1‑150 (MQ=255)
                                                                gATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATAAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTg                                                                                       >  1:731859/1‑150 (MQ=255)
                                                                 aTCGCAGAAATCCTTTTGCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACGGc                                                                                      <  2:583534/150‑1 (MQ=255)
                                                                 aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTCTTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGc                                                                                      <  1:900761/150‑1 (MQ=255)
                                                                   cGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                                    >  2:900403/1‑150 (MQ=255)
                                                                     cAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTAACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCACTTCAGAAGCAAAACATACAAACTGCacac                                                                                  >  2:568029/1‑150 (MQ=255)
                                                                      aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacg                                                                                 <  1:820128/150‑1 (MQ=255)
                                                                       taaaGCCTTTTTCTTCCAGACTTCTATGATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACTAACTGCccacgc                                                                                <  1:784151/149‑1 (MQ=255)
                                                                        aaaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                               <  1:1462279/150‑1 (MQ=255)
                                                                        aaaGCCTTTTTCTTCCAGACTTCTATAATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                               <  1:338620/150‑1 (MQ=255)
                                                                          aGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                             <  1:1862235/150‑1 (MQ=255)
                                                                              tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGt                                                                         <  1:764291/150‑1 (MQ=255)
                                                                               ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGtt                                                                        >  1:1353470/1‑150 (MQ=255)
                                                                                tttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGGTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtg                                                                       <  1:1427696/150‑1 (MQ=255)
                                                                                   cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTgtgt                                                                    >  2:438102/1‑150 (MQ=255)
                                                                                    ttcCAGACTTCTATCATGGCGTAAAAATCAAATAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                                   <  2:1457873/150‑1 (MQ=255)
                                                                                    ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCATACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                                   <  1:1492496/150‑1 (MQ=255)
                                                                                    ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                                   <  1:550809/150‑1 (MQ=255)
                                                                                      ccAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGa                                                                 >  2:1415882/1‑150 (MQ=255)
                                                                                         gACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTATATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTACAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGAAGAtgt                                                              >  1:1056029/1‑150 (MQ=255)
                                                                                          aCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgtg                                                             <  1:789044/150‑1 (MQ=255)
                                                                                                  cATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCAGCGTTACTTCCGGGCGGCAGTTCAGACTCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGc                                                     >  1:283619/1‑150 (MQ=255)
                                                                                                    tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCa                                                   <  1:604134/150‑1 (MQ=255)
                                                                                                       cGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTAGTGAAGATGTCTCTTTCGCCAAAg                                                >  2:1306020/1‑150 (MQ=255)
                                                                                                           aaaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTACAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGAAGATGTGTCTTTCGCCAAAGGCAt                                            >  2:1270821/1‑150 (MQ=255)
                                                                                                                cAAAAAATTATTTTTTTTATTGTGTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAAt                                       <  2:1660760/150‑1 (MQ=255)
                                                                                                                cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAAt                                       >  1:1722635/1‑150 (MQ=255)
                                                                                                                cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAAGATCTACACCAGTTCCAGCATCGTTACTTCAGGGCGGAAGTTCAGACGCAAAAAATACAAACTGCCCACTCCACGGGTTGTGAAGATGTGTCTTTCGCCAAAGGCATTTAAt                                       >  1:259441/1‑150 (MQ=255)
                                                                                                                  aaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATATACACCAGTTCCAGCATCGTTACTTCCGTGCGGCAGTTCAGACGAAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATcc                                     >  1:1837818/1‑150 (MQ=255)
                                                                                                                       ttACCTGCTTTATTCAGGTGAGAAAATTCACGATCTACACCATTTCCAGCTTCTTTACTTCCGGGCGGCAGTTCAGAGGCAAACCATACAAACTGCCGACCCCACGGGTTGGGTAGATGGGTCTTTCGCCAAAGGGATTTAATCCGGCGa                                <  1:300743/150‑1 (MQ=255)
                                                                                                                           tttatttATTCTTTTGTAAAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTa                            <  2:1723517/146‑1 (MQ=255)
                                                                                                                            tGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCCCCGGTTGTGTAGATGTGTCTTTCGCCAAAGGCAATTATTCGGCCGACGTaa                           >  2:306834/1‑150 (MQ=255)
                                                                                                                              cTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCTGCAGTTAAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg                         >  1:1455844/1‑150 (MQ=255)
                                                                                                                                ttATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGtt                       >  2:419739/1‑150 (MQ=255)
                                                                                                                                   ttCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTAt                    >  2:2040194/1‑150 (MQ=255)
                                                                                                                                     cTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCt                  <  1:134244/150‑1 (MQ=255)
                                                                                                                                     cTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTACATCTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAAGTTTAACt                  >  2:1247625/1‑150 (MQ=255)
                                                                                                                                       ggTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGGAGTTCAGACGCAAACCATACAACCTGCCCACGCCCCGGGGTGTGTAGATGTGTCTTTCGCCAAAGGCAGTTAATCCGGCGACGTAACGTGTATCTTc                >  1:342219/1‑150 (MQ=255)
                                                                                                                                       ggTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCACACCATACAAACTGCCCACGCCCCGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCtta                >  1:951025/1‑149 (MQ=255)
                                                                                                                                       ggTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTc                <  1:665873/150‑1 (MQ=255)
                                                                                                                                       ggTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTc                >  1:768937/1‑150 (MQ=255)
                                                                                                                                         tGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTACAGACGCAAACCATACAAACAGCCCACGCCACGGGTTGTGTAGATGTCTCTTACACCAAACGCATTAAAACACTCCACGTAACGTTTATCTACgc              >  2:201577/1‑149 (MQ=255)
                                                                                                                                          gATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCTCGGGGTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCGGGCGACGAAACGTTTAtatacgca             >  1:1330907/1‑143 (MQ=255)
                                                                                                                                          gATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACATCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAc             >  1:1094437/1‑150 (MQ=255)
                                                                                                                                              aaaTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAggg         <  2:1970633/150‑1 (MQ=255)
                                                                                                                                                    aCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCaaaa   <  1:1317048/150‑1 (MQ=255)
                                                                                                                                                    aCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCaaaa   <  1:900404/150‑1 (MQ=255)
                                                                                                                                                     cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAg  <  1:757765/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACAGGGGCAAAAG  >  NC_000913/184104‑184402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: