Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,687 C→A 5.0% L22M (CTG→ATG)  yohJ → UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,6870CA 5.0% 344.5 / 4.3 117L22M (CTG→ATG) yohJUPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (42/68);  new base A (4/3);  total (46/71)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

AAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATAT  >  NC_000913/2230538‑2230835
                                                                                                                                                     |                                                                                                                                                    
aaaTAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTAtgcctgcc                                                                                                                                                      <  2:1526969/150‑1 (MQ=255)
aaaTAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTAtgcctgcc                                                                                                                                                      >  2:894397/1‑150 (MQ=255)
  attaGCGCTGATTTCATAATGTGAGCGGTAGCACGTTTTACGACTTAATTGTATGATGAATCCATCTGATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTTTCTGGCAATATTTCCGCGCTTTCGTCCTGATATATGcctgcctg                                                                                                                                                    <  2:595010/147‑1 (MQ=255)
    aaGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTa                                                                                                                                                  <  2:1494509/150‑1 (MQ=255)
       cgcTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGc                                                                                                                                               <  2:401076/150‑1 (MQ=255)
        gcTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCa                                                                                                                                              <  1:965341/150‑1 (MQ=255)
          tGATTTCTTAATGTGCTCGGTAGCACGTTTTACGAATTAATTTTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTAACTGCCAAAATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAgg                                                                                                                                            <  2:537933/150‑1 (MQ=255)
          tGATTTCTTAATGTGATCGGTAGCTCGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAgg                                                                                                                                            <  2:1303816/150‑1 (MQ=255)
          tGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAgg                                                                                                                                            <  2:1888326/150‑1 (MQ=255)
          tGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAgg                                                                                                                                            <  2:848100/150‑1 (MQ=255)
          tGAATTCTTAAAGTGATCGCTACCACGTTTTACGAATTAAATGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAgg                                                                                                                                            <  2:1736082/150‑1 (MQ=255)
           gATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGc                                                                                                                                           >  1:713186/1‑150 (MQ=255)
             tttCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGTGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCAt                                                                                                                                         <  2:1714583/150‑1 (MQ=255)
             tttCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATACATCTAATCTGGGGTGTTGATTATGAGTAAGAAAATGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATTTATGCAGTCAt                                                                                                                                         >  1:1716098/1‑150 (MQ=255)
               tCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCAttt                                                                                                                                       >  2:1662766/1‑150 (MQ=255)
                cTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCAtttt                                                                                                                                      >  2:595920/1‑150 (MQ=255)
                cTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATGTATGCAGGCAtttt                                                                                                                                      >  1:1591451/1‑150 (MQ=255)
                 ttAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTc                                                                                                                                     >  2:1104610/1‑150 (MQ=255)
                  tAATTTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                                    <  1:152109/150‑1 (MQ=255)
                  tAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                                    <  2:728052/150‑1 (MQ=255)
                  tAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatc                                                                                                                                    <  2:1138894/150‑6 (MQ=255)
                  tAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatc                                                                                                                                    <  2:1138989/150‑6 (MQ=255)
                   aaTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCAt                                                                                                                                   <  1:595657/150‑1 (MQ=255)
                     tgtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTg                                                                                                                                 <  1:670160/150‑1 (MQ=255)
                      ttgATAGGTTGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                                <  2:1791565/149‑1 (MQ=255)
                      gtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                                <  2:1836301/150‑1 (MQ=255)
                      gtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                                >  2:1427068/1‑150 (MQ=255)
                      gtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTAGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                                >  1:1022699/1‑150 (MQ=255)
                          tCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                            >  2:1432093/1‑150 (MQ=255)
                             gTAGCACGTTTTAAGAATTAATTGTATTATGAATCAATCTCATCTGGGGTTTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGAATTTTCATTGCTTCCCTg                                                                                                                         <  1:904884/150‑1 (MQ=255)
                               aGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCt                                                                                                                       >  1:1607716/1‑150 (MQ=255)
                                gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTa                                                                                                                      >  1:848976/1‑150 (MQ=255)
                                gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATATATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTa                                                                                                                      <  2:1707609/150‑1 (MQ=255)
                                gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGAAACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTa                                                                                                                      >  2:1304978/1‑150 (MQ=255)
                                 cACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTAc                                                                                                                     <  2:880159/150‑1 (MQ=255)
                                  aCGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTAcc                                                                                                                    <  1:1702334/150‑1 (MQ=255)
                                  aCGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTAcc                                                                                                                    <  1:1453702/150‑1 (MQ=255)
                                    gTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCgg                                                                                                                  <  2:1603307/150‑1 (MQ=255)
                                        tACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                              >  1:1160123/1‑150 (MQ=255)
                                         aCGAATTAATTGTATGATGAATCCATCTTATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAcc                                                                                                             <  1:905766/150‑1 (MQ=255)
                                             attaattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTc                                                                                                         <  1:1308439/150‑1 (MQ=255)
                                                aattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGCCACTGAACATTATCTGGCAATATTTACGCGGTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCgg                                                                                                      >  2:28545/1‑150 (MQ=255)
                                                aattGTATGATGAATCCATCTCATCTGGGGTGTTGAGTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCgg                                                                                                      >  2:519772/1‑150 (MQ=255)
                                                  ttGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgc                                                                                                    <  1:1807036/150‑1 (MQ=255)
                                                    gTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgcag                                                                                                  >  1:1166095/1‑150 (MQ=255)
                                                    gTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTACTACCGGTAACCATTCCGGgcag                                                                                                  <  1:1953994/150‑1 (MQ=255)
                                                      atgatgAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgcagca                                                                                                <  1:1986490/150‑1 (MQ=255)
                                                       tgatgaATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTCCTTCCCTGCTACCGGTAACCATTCCGGGCAGcat                                                                                               >  1:1624779/1‑150 (MQ=255)
                                                            aaTCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCg                                                                                          <  1:1323275/150‑1 (MQ=255)
                                                             aTCCATCTCATGTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGGATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTTACCATTCCGGGCAGCATCATCgg                                                                                         <  1:1410262/150‑1 (MQ=255)
                                                             aTCCATCTCATCTGGTGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCgg                                                                                         <  1:1530569/150‑1 (MQ=255)
                                                                catctcatctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGAt                                                                                      <  1:937015/150‑1 (MQ=255)
                                                                 atctcatctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATg                                                                                     >  2:1808554/1‑150 (MQ=255)
                                                                  tctcatctGGGGTGTTGAATATGAGTAAGCCACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTCATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATGCCGGGCAGCATCATCCGGTTGc                                                                                    >  2:1897579/1‑150 (MQ=255)
                                                                     catctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATGTATGCAGGCATTTTCATTGCTTACCTGCTACAGGTAACCATTCAGGGCAGCATAATCGGGATGCTGa                                                                                 >  1:1206399/1‑150 (MQ=255)
                                                                      atctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGAt                                                                                >  2:70668/1‑150 (MQ=255)
                                                                       tctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATc                                                                               <  2:1590320/150‑1 (MQ=255)
                                                                        ctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTCTGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATcc                                                                              <  1:1190300/150‑1 (MQ=255)
                                                                         tGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCt                                                                             <  1:1624757/150‑1 (MQ=255)
                                                                            ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                          <  1:1734201/150‑1 (MQ=255)
                                                                            ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAACATTCCGGGCAGCATAATCGGTATGCTGATCCTGtt                                                                          >  1:827737/1‑150 (MQ=255)
                                                                            ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCATGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                          <  1:424123/150‑1 (MQ=255)
                                                                            ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTAGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAACATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                          >  1:132487/1‑150 (MQ=255)
                                                                                 tGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTcc                                                                     <  2:1250015/150‑1 (MQ=255)
                                                                                 tGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTcc                                                                     <  1:1435927/150‑1 (MQ=255)
                                                                                 tGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTcc                                                                     <  2:507623/150‑1 (MQ=255)
                                                                                 tGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTcc                                                                     <  2:541630/150‑1 (MQ=255)
                                                                                 tGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTcc                                                                     <  2:778417/150‑1 (MQ=255)
                                                                                   aTTATGAGTAAGACACTGAACATTTTCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCATGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctg                                                                   >  1:372573/1‑150 (MQ=255)
                                                                                   aTTATGAGTAAGACACTGAACATTATCTTGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctg                                                                   <  2:943244/150‑1 (MQ=255)
                                                                                    ttATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctgc                                                                  <  2:1587783/150‑1 (MQ=255)
                                                                                    ttATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctgc                                                                  <  1:1460608/150‑1 (MQ=255)
                                                                                    ttATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctgc                                                                  <  2:282627/150‑1 (MQ=255)
                                                                                       tGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTgg                                                               >  1:1098425/1‑150 (MQ=255)
                                                                                          gTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCGTGTTTGTCCTGCTGGCCt                                                            >  1:125031/1‑150 (MQ=255)
                                                                                              gACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCa                                                        >  1:454429/1‑150 (MQ=255)
                                                                                                 acTGAACATTCACTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCGGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAt                                                     <  1:1257277/150‑1 (MQ=255)
                                                                                                  cTGAACCTTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAtt                                                    <  2:1559083/150‑1 (MQ=255)
                                                                                                  cTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTTTATGCAGGAATTTTCATTGCTTCCCTGCTAACGGTAACCATTCCGGGCAGCATCATCGGGATTCTGATCCTGTTTTTCATGCTGGCCTTGCAAAtt                                                    >  2:1204490/1‑150 (MQ=255)
                                                                                                     aaCATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCTGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAttctt                                                 <  1:1400736/150‑1 (MQ=255)
                                                                                                        aTTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCg                                              <  2:810007/150‑1 (MQ=255)
                                                                                                         ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                             <  1:1662767/150‑1 (MQ=255)
                                                                                                         ttAGCTGGCTATATTTACGCGCTTTCGTCCTGATATATTCCTGGCTGTATGGAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCATATTCTTcctg                                             <  1:311396/150‑3 (MQ=255)
                                                                                                         taaTCTGGCAATAGTTCCGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                             <  2:93883/148‑1 (MQ=255)
                                                                                                             gtGGCAATCTTTACGCGCTTTCGGCCTGATTTATGCCTGCCTGAATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCaaa                                         <  2:1022452/149‑1 (MQ=255)
                                                                                                             cTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCaaa                                         >  2:1662537/1‑150 (MQ=255)
                                                                                                                   atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGt                                   <  2:694150/150‑1 (MQ=255)
                                                                                                                        tACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATc                              <  2:2012964/150‑1 (MQ=255)
                                                                                                                                   gTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTAc                   >  2:1918773/1‑150 (MQ=255)
                                                                                                                                      cTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTa                >  2:108842/1‑150 (MQ=255)
                                                                                                                                       tGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTAc               <  2:713185/150‑1 (MQ=255)
                                                                                                                                         aTTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTg             >  1:719668/1‑150 (MQ=255)
                                                                                                                                         aTTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAACATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTg             <  2:1022698/150‑1 (MQ=255)
                                                                                                                                          tttATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGa            >  1:542003/1‑150 (MQ=255)
                                                                                                                                          tttATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTAAGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGa            >  1:655070/1‑150 (MQ=255)
                                                                                                                                             aTGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGGTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTc         >  2:412430/1‑150 (MQ=255)
                                                                                                                                              tgcctgccTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCg        <  1:1548746/150‑1 (MQ=255)
                                                                                                                                                cctgcctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCt      >  1:1816792/1‑150 (MQ=255)
                                                                                                                                                   gccttTATGCAGGCATTTTCATTGCATCCCTGCTACAGGTAACCAATCCGGGCAGCATCAGCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCtata   <  2:326570/150‑1 (MQ=255)
                                                                                                                                                    cctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTatat  <  2:1166094/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATAAGCGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATAT  >  NC_000913/2230538‑2230835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: