Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,252,814 C→A 5.7% intergenic (+26/+81) yeiI → / ← nupX putative kinase/nucleoside permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,252,8140CA 5.7% 372.9 / 9.5 124intergenic (+26/+81)yeiI/nupXputative kinase/nucleoside permease
Reads supporting (aligned to +/- strand):  ref base C (50/67);  new base A (5/2);  total (55/69)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01

Only 100 of 132 total aligned reads displayed.

TACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCC  >  NC_000913/2252666‑2252961
                                                                                                                                                    |                                                                                                                                                   
tACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAcg                                                                                                                                                    >  2:411475/1‑150 (MQ=255)
tACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAcg                                                                                                                                                    >  1:1657847/1‑150 (MQ=255)
  cAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACCACCCTACCCTGTCTGCCGATAACGCGCGTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAcgcg                                                                                                                                                  >  1:2041243/1‑150 (MQ=255)
  cAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACACCCCTCCCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAcgcg                                                                                                                                                  >  1:690030/1‑150 (MQ=255)
  cAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCGACCCTGTCTGCCGATAAAGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTTCCGGATGCGACGCTTAAcgcg                                                                                                                                                  >  2:799764/1‑150 (MQ=255)
   aGTTTCCGCGACAGCGCCAGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTTGTGCCAATTGGGTAACAATGTTGCCGGATGCGACGCTTAACGCGt                                                                                                                                                 <  1:1245428/150‑1 (MQ=255)
    gTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTc                                                                                                                                                <  2:465241/150‑1 (MQ=255)
        ccGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCGTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTAt                                                                                                                                            >  1:813233/1‑150 (MQ=255)
        ccGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCAGATAACGCGCTTTCATTAGAGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTAt                                                                                                                                            >  1:1575306/1‑150 (MQ=255)
          gcgACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATcc                                                                                                                                          <  2:817091/150‑1 (MQ=255)
             aCAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGc                                                                                                                                       <  2:696123/150‑1 (MQ=255)
              cAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGcc                                                                                                                                      <  1:644827/150‑1 (MQ=255)
              cAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATAAGACGCGTTAAGCGTCGCATCCGGca                                                                                                                                      <  1:854594/150‑2 (MQ=255)
              cAGCGCCCGGTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGTTAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGcc                                                                                                                                      <  2:1396976/150‑1 (MQ=255)
                gcgcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTa                                                                                                                                    <  2:539648/150‑1 (MQ=255)
                 cgcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTAc                                                                                                                                   <  1:1340132/150‑1 (MQ=255)
                 cgcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTAc                                                                                                                                   >  1:857640/1‑150 (MQ=255)
                  gcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACa                                                                                                                                  <  1:1922539/150‑1 (MQ=255)
                  gcCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACa                                                                                                                                  >  2:1031318/1‑150 (MQ=255)
                    ccGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACaaa                                                                                                                                >  2:1047670/1‑150 (MQ=255)
                         ttGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAgg                                                                                                                           <  1:1925085/150‑1 (MQ=255)
                          tGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGc                                                                                                                          >  2:1801684/1‑150 (MQ=255)
                           gCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCa                                                                                                                         >  2:1158401/1‑150 (MQ=255)
                              gTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAAtt                                                                                                                      <  2:1967626/150‑1 (MQ=255)
                              gTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAAtt                                                                                                                      <  2:731404/150‑1 (MQ=255)
                                aGCCTGCGCGGCAATTTCGCTCTCGATCGGCTGCTTAAACAACCCTACCCTGTCTGCCGTAATCGCGCTTTCAATAGTGCCAATGGTGTAACAATGTTGCGGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTc                                                                                                                    <  2:141754/150‑1 (MQ=255)
                                  ccTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAg                                                                                                                  <  1:677457/150‑1 (MQ=255)
                                  ccTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAg                                                                                                                  >  2:2037376/1‑150 (MQ=255)
                                  ccTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAg                                                                                                                  >  2:1132055/1‑150 (MQ=255)
                                    tGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCa                                                                                                                <  1:561071/150‑1 (MQ=255)
                                      cgcgGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATATGTGCCAATGGGGTCACAATGTTGCCGGATGCGACGCTGAACGCGTCTTATCCGGCCGACAAAACAGGCAATTTCAGCAAg                                                                                                              >  1:393785/1‑150 (MQ=255)
                                           cAATATCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTCTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAAt                                                                                                         <  1:559375/150‑1 (MQ=255)
                                             aTTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGt                                                                                                       <  2:857639/150‑1 (MQ=255)
                                                tCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAgg                                                                                                    <  2:1053279/150‑1 (MQ=255)
                                                 cgcgcgCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGc                                                                                                   >  2:1953673/1‑150 (MQ=255)
                                                 cgcgcgCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGc                                                                                                   >  2:1202996/1‑150 (MQ=255)
                                                        cAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATaa                                                                                            <  1:1728349/150‑1 (MQ=255)
                                                         aGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAg                                                                                           >  2:279380/1‑150 (MQ=255)
                                                                gCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTgcgc                                                                                    <  2:1428086/150‑1 (MQ=255)
                                                                 cTTAAACAACCCTACCCTGTCTGCCGATACCGCGCTTTCATTAGTGCCAATGGTGTACCAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCa                                                                                   >  2:394636/1‑150 (MQ=255)
                                                                 cTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCa                                                                                   >  2:2024989/1‑150 (MQ=255)
                                                                   tAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGACAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGACTACATATCAGGCAATTTCCGCCAGAAAAAGTAGGCCTGATAATCTTGAGTATc                                                                                 >  2:299442/1‑150 (MQ=255)
                                                                   tAAACAACCCTACCCTGTCTGCCCATAACGCGAATTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCATGATAAGCTTGCGCATc                                                                                 >  1:31800/1‑150 (MQ=255)
                                                                       caacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGc                                                                             <  1:1800824/150‑1 (MQ=255)
                                                                       caacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGc                                                                             <  2:256716/150‑1 (MQ=255)
                                                                       caacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGc                                                                             <  2:1867503/150‑1 (MQ=255)
                                                                       caacCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGAGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGc                                                                             <  1:1982942/150‑1 (MQ=255)
                                                                         accctaccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCaa                                                                           <  1:770131/150‑1 (MQ=255)
                                                                           cctaccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATg                                                                         >  2:47214/1‑150 (MQ=255)
                                                                            ctaccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTTTCCGGCCTACAAATCAGGCAATTTCAGAAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGa                                                                        >  1:1789094/1‑150 (MQ=255)
                                                                            ctaaactGTCTGACGAAAACCCTCTTTCATTATTTCCAATGGAGTAAGAATGTTGCCGGATGAGAAGCTTAACTCGTCTTATCCGGGCTACAAATCAGGCAATTGCAGCAAGTAAATGCAGGCCTGATAAGCTTGCGCATCACGCAATGa                                                                        <  1:338914/145‑1 (MQ=255)
                                                                               ccctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGacac                                                                     <  1:277397/150‑1 (MQ=255)
                                                                                cctGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACt                                                                    >  1:400450/1‑150 (MQ=255)
                                                                                   gTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCaa                                                                 >  2:1069269/1‑150 (MQ=255)
                                                                                    tCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAg                                                                <  1:2033775/150‑1 (MQ=255)
                                                                                    tCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAg                                                                >  2:446555/1‑150 (MQ=255)
                                                                                    tCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAg                                                                >  2:453915/1‑150 (MQ=255)
                                                                                     cTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGc                                                               <  1:486982/150‑1 (MQ=255)
                                                                                      tGCAGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCt                                                              <  1:739991/150‑1 (MQ=255)
                                                                                       gCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTa                                                             >  2:644781/1‑150 (MQ=255)
                                                                                       gCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTa                                                             <  2:754697/150‑1 (MQ=255)
                                                                                       gCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTa                                                             <  1:1046085/150‑1 (MQ=255)
                                                                                        ccGATAACGCGCTTTCATTTGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTaa                                                            >  1:895327/1‑150 (MQ=255)
                                                                                          gATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAAc                                                          <  1:239489/150‑1 (MQ=255)
                                                                                           aTAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCTATGACACTCAAGCTAAAcc                                                         <  2:71255/150‑1 (MQ=255)
                                                                                           aTAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAAcc                                                         <  1:95544/150‑1 (MQ=255)
                                                                                           aTAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAAcc                                                         <  1:392962/150‑1 (MQ=255)
                                                                                              aCGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAAt                                                      >  1:239718/1‑150 (MQ=255)
                                                                                              aCGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAAt                                                      >  1:298384/1‑150 (MQ=255)
                                                                                                gcgcTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATaa                                                    <  1:1417096/150‑1 (MQ=255)
                                                                                                  gcTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAg                                                  <  1:1890375/150‑1 (MQ=255)
                                                                                                       cATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAAccc                                             >  2:207490/1‑150 (MQ=255)
                                                                                                          tAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGc                                          <  1:411475/150‑1 (MQ=255)
                                                                                                            gtgtCATTGGTGTAACAAAGTTGACGGATGAGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCaa                                        <  1:339245/150‑1 (MQ=255)
                                                                                                              gCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATCCGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCACTAAAGACCCCGCCAATg                                      >  1:1227133/1‑150 (MQ=255)
                                                                                                              gCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATg                                      >  2:1690590/1‑150 (MQ=255)
                                                                                                                  aTGGTGTAACAATGTTGCCGGATGCGACGCTTAACTCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCg                                  <  1:1349730/150‑1 (MQ=255)
                                                                                                                   tGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCTATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGAAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGc                                 >  1:1968664/1‑150 (MQ=255)
                                                                                                                     gtgtAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCAc                               >  2:1704124/1‑150 (MQ=255)
                                                                                                                      tgtAACTATGTTGCAGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGCCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAATGAACCCGGCAATGGTCGCACt                              <  2:756189/150‑1 (MQ=255)
                                                                                                                      tgtAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACt                              <  2:2041242/150‑1 (MQ=255)
                                                                                                                       gtAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACtc                             <  1:423143/150‑1 (MQ=255)
                                                                                                                          aCAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCACGCTAAACCAATAAAGAACCCGGCAATGGTCGCACtcatc                          >  1:541260/1‑150 (MQ=255)
                                                                                                                              tGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGt                      <  2:111412/150‑1 (MQ=255)
                                                                                                                               gTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGCCACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGtt                     >  2:1263745/1‑150 (MQ=255)
                                                                                                                                aggCCGGATAAGACGCGTTAAGCGTCGCATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTg                    >  1:6571/3‑150 (MQ=255)
                                                                                                                                 ggCCGGATAAGACGCGTTAAGCGTCGCATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTgg                   >  1:2034065/2‑150 (MQ=255)
                                                                                                                                  gCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGa                  >  2:1977628/1‑150 (MQ=255)
                                                                                                                                   ccGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCCGGCAATGACACTCAAGCTACACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGaa                 >  2:1793406/1‑150 (MQ=255)
                                                                                                                                    cGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGACCCCGGCAATGGTCGCACTCATCAAGTTGGaaa                >  1:1970736/1‑150 (MQ=255)
                                                                                                                                    cGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGaaa                <  1:1031319/150‑1 (MQ=255)
                                                                                                                                     ggataagACGCGTTAAGCGTCGCATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCCCTCATCAAGTTGGAAAg               >  1:1209232/7‑150 (MQ=255)
                                                                                                                                        tGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGt            <  2:84875/150‑1 (MQ=255)
                                                                                                                                          cGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcg          >  2:1192208/1‑150 (MQ=255)
                                                                                                                                              gCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgc      <  1:1801685/150‑1 (MQ=255)
                                                                                                                                              gCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgc      <  2:1008568/150‑1 (MQ=255)
                                                                                                                                               cTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgcc     <  2:680571/150‑1 (MQ=255)
                                                                                                                                                ttAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAACTGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgccg    <  1:1307194/150‑1 (MQ=255)
                                                                                                                                                ttAACGCGTCTTATCCGGCCTACAAATCACGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCGTGCGCATCAGGCAATGAGACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTcgccgccg    <  1:1265194/150‑1 (MQ=255)
                                                                                                                                                  aaCGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCgccgccgcc  <  2:1694917/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGCAATTTCAGCAAGTAAATGTAGGCCTGATAAGCTTGCGCATCAGGCAATGACACTCAAGCTAAACCAATAAAGAACCCGGCAATGGTCGCACTCATCAAGTTGGAAAGCGTCGCCGCCGCC  >  NC_000913/2252666‑2252961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: