Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,270,602 T→C 5.6% intergenic (+57/‑124) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,270,6020TC 5.6% 435.7 / 11.4 143intergenic (+57/‑124)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (73/61);  new base C (6/2);  total (80/63)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 149 total aligned reads displayed.

CCACCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGG  >  NC_000913/2270454‑2270751
                                                                                                                                                    |                                                                                                                                                     
ccaccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCtt                                                                                                                                                      <  2:100690/150‑1 (MQ=255)
ccaccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCtt                                                                                                                                                      >  2:1444465/1‑150 (MQ=255)
 caccaGCAGTGGTGTTTTTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTAAAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTAGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTa                                                                                                                                                     >  2:1035421/1‑150 (MQ=255)
 caccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTa                                                                                                                                                     >  2:1918954/1‑150 (MQ=255)
 caccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCACTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTa                                                                                                                                                     >  2:1494450/1‑150 (MQ=255)
   ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg                                                                                                                                                   >  2:1995522/1‑150 (MQ=255)
   ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg                                                                                                                                                   <  1:1613196/150‑1 (MQ=255)
    cagcagTGGTGTTATAATTTCCAGCATTCATGAACCTTACTGGAAGAAGCGTTAAAACGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGAAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGc                                                                                                                                                  <  2:591234/150‑1 (MQ=255)
     agcagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCa                                                                                                                                                 <  1:45901/150‑1 (MQ=255)
         gtggtgGTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTGGGCGATCCTGACGAGTTAACTGAAAGCACGGCTTAGGCAGTGc                                                                                                                                             >  1:393330/1‑150 (MQ=255)
             tgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttt                                                                                                                                         >  2:938451/1‑150 (MQ=255)
             tgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttt                                                                                                                                         <  2:570269/150‑1 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGACGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                        >  1:65489/1‑150 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCATTGCttttt                                                                                                                                        >  1:994186/1‑150 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                        <  2:1424871/150‑1 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                        >  1:700025/1‑150 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                        >  2:1818883/1‑150 (MQ=255)
              gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttca                                                                                                                                        <  1:16608/150‑3 (MQ=255)
                tattatTTCCAGCATGAATGAACCGTACTGGAAGACGCGGTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                      <  1:1043284/150‑1 (MQ=255)
                tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGTATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                      <  2:1061160/150‑1 (MQ=255)
                 attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAATCCGTTTGGATGCAATCCCTTGGCTATCCTGCCGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGt                                                                                                                                     >  2:1217463/1‑150 (MQ=255)
                 attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGt                                                                                                                                     >  2:1422392/1‑150 (MQ=255)
                 attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGt                                                                                                                                     >  1:1382096/1‑150 (MQ=255)
                    attTCCAGCATGAATGAACCGTACGGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtttt                                                                                                                                  >  1:1007791/1‑150 (MQ=255)
                      ttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttca                                                                                                                                <  1:1687110/150‑1 (MQ=255)
                       tCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcat                                                                                                                               <  2:88331/150‑1 (MQ=255)
                       tCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTGGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcat                                                                                                                               <  1:281864/150‑1 (MQ=255)
                        ccAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTGAGGCAGTGCTTTGTTGTTttcatt                                                                                                                              >  1:210718/1‑150 (MQ=255)
                           gCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTtcattcat                                                                                                                           >  1:1554648/1‑150 (MQ=255)
                            cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                          <  1:1213336/150‑1 (MQ=255)
                            cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                          <  1:1729299/150‑1 (MQ=255)
                            cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCGTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                          >  2:343677/1‑150 (MQ=255)
                                 atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAgaga                                                                                                                     >  2:1410958/1‑150 (MQ=255)
                                  tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaa                                                                                                                    >  1:859408/1‑150 (MQ=255)
                                  tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaa                                                                                                                    >  1:631255/1‑150 (MQ=255)
                                         tACTGCAAGAAGCGGTTCAACGAACCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTTTCCTGACGAGTTAACTGAAAGCTCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgatg                                                                                                             <  2:1667288/150‑1 (MQ=255)
                                             ggcagaagCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                         <  2:806099/147‑1 (MQ=255)
                                             ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCCGCTTATAAGCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCGTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                         >  2:1195187/1‑150 (MQ=255)
                                             ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                         >  2:1117280/1‑150 (MQ=255)
                                               aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                       <  1:1564660/150‑1 (MQ=255)
                                               aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                       <  1:137339/150‑1 (MQ=255)
                                               aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATACTGACGAGTTAAATGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                       >  1:1527224/1‑150 (MQ=255)
                                                agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGGTTTTTTGTTTTCATTATTCAGAGAAATTGATGTTTCagc                                                                                                      >  2:1897014/1‑150 (MQ=255)
                                                agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgc                                                                                                      >  2:1904719/1‑150 (MQ=255)
                                                    gCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCt                                                                                                  <  1:409189/150‑1 (MQ=255)
                                                     cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                                                                 <  1:1814125/150‑1 (MQ=255)
                                                     cGTTACAACGAAGCACGCCGGGTTCTCAGCAGCAGCTAATAAAACGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                                                                 <  2:515768/150‑1 (MQ=255)
                                                      gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTg                                                                                                >  1:505291/1‑150 (MQ=255)
                                                           aaCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCa                                                                                           >  2:536763/1‑150 (MQ=255)
                                                            aCGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAg                                                                                          <  1:1258480/150‑1 (MQ=255)
                                                             cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAgg                                                                                         <  1:489747/150‑1 (MQ=255)
                                                                    cGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGt                                                                                  >  1:85026/1‑150 (MQ=255)
                                                                      ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGATTAGGCAGTGCTTTTTTGTTTACATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATACAGGGTATAGTcc                                                                                >  2:1016870/1‑150 (MQ=255)
                                                                        gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGATATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg                                                                              <  1:1345370/150‑1 (MQ=255)
                                                                               cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGt                                                                       <  2:1032252/150‑1 (MQ=255)
                                                                                 gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTTCTTTTTTGTTTTCATTCATCAGAGAAATTGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                                                                     <  1:1035422/150‑1 (MQ=255)
                                                                                   cGCTGCTAATAAACCGGTTGGATGCAATCCCTAGGCAATCCTGACGAGTTAACTGACAGGACTGCTTAGGCAGTGCTTTTTTGTTTTCATCCATCAGAGAACATGAGGTTTCCGCGTCTTGATCCCGGCTATAGTCCGGGCATTGTTATc                                                                   <  2:295690/150‑1 (MQ=255)
                                                                                        cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTa                                                              >  1:367803/1‑150 (MQ=255)
                                                                                         taataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGATAATGATGTTTCCGCGTCTTGATCCAGGCTATATTCCGGTCATTGTTATCTTTTaa                                                             <  2:1150087/150‑1 (MQ=255)
                                                                                               aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttg                                                       >  2:1469387/1‑150 (MQ=255)
                                                                                                       gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCGTTTAAATGTTGTCGTAAtt                                               >  2:809267/1‑150 (MQ=255)
                                                                                                        aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt                                              <  2:1043877/150‑1 (MQ=255)
                                                                                                         tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATGAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTc                                             <  2:1505478/150‑1 (MQ=255)
                                                                                                          gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa                                            <  1:1991429/150‑1 (MQ=255)
                                                                                                           cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGATAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                           <  1:756686/150‑1 (MQ=255)
                                                                                                           cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                           >  2:600313/1‑150 (MQ=255)
                                                                                                           cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                           >  1:1320783/1‑150 (MQ=255)
                                                                                                           cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                           >  2:169898/1‑150 (MQ=255)
                                                                                                            aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg                                          <  1:523871/150‑1 (MQ=255)
                                                                                                            aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg                                          >  2:1573624/1‑150 (MQ=255)
                                                                                                             aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCTCGTCTTGATCCAGGATATAGTCCGGTCATTGTTATCTTTTAAATGTTTTCGTAATTTCAGGa                                         >  1:1611078/1‑150 (MQ=255)
                                                                                                              tCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATAGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa                                        >  1:1253792/1‑150 (MQ=255)
                                                                                                                ccTTGGCTATCCTTACGAGATAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTATCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                      <  1:338366/150‑1 (MQ=255)
                                                                                                                    ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc                                  >  2:1301402/1‑150 (MQ=255)
                                                                                                                     gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg                                 <  1:1221055/150‑1 (MQ=255)
                                                                                                                          ccTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCCGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATAAACGGAAta                            >  2:1533734/1‑149 (MQ=255)
                                                                                                                          ccTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATc                            >  2:905960/1‑150 (MQ=255)
                                                                                                                           cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                           >  2:1370395/1‑150 (MQ=255)
                                                                                                                           cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                           >  2:1363473/1‑150 (MQ=255)
                                                                                                                            tGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTTTCGTAATTTCAGGAAATTAACGGATTCAt                          >  1:1961335/1‑150 (MQ=255)
                                                                                                                               cGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                       >  1:1124703/1‑150 (MQ=255)
                                                                                                                                gAGTTTACTGTAAGCTCTGCTTAGTCTGTGTTTTTTTTTTTTCATTCATCTGAGAAAATCATGTTTCAGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATTTTc                      <  2:1245436/150‑1 (MQ=255)
                                                                                                                                 aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                     <  2:1413592/150‑1 (MQ=255)
                                                                                                                                 aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                     <  2:85026/150‑1 (MQ=255)
                                                                                                                                  gTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                    >  2:454917/1‑150 (MQ=255)
                                                                                                                                   ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATa                   <  2:700024/150‑1 (MQ=255)
                                                                                                                                   aaacaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATa                   >  2:572103/8‑150 (MQ=255)
                                                                                                                                    tAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                  >  1:1844877/1‑150 (MQ=255)
                                                                                                                                     aaCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcg                 >  1:267574/1‑150 (MQ=255)
                                                                                                                                      caaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc                >  2:1081360/5‑150 (MQ=255)
                                                                                                                                       cTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGTCTATAGTCCGGTCATTGTGATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgcc               >  2:1793342/1‑149 (MQ=255)
                                                                                                                                          aaaGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGGCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgcgc            <  1:2015278/150‑3 (MQ=255)
                                                                                                                                           aaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCGTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAGCATGTTCATACGCGCTcc           >  2:304166/1‑150 (MQ=255)
                                                                                                                                            aGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATACAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAAGCGCTcgc          >  1:148381/1‑148 (MQ=255)
                                                                                                                                              cACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATATTATAAATGTTGTCGTAATTTCAGTAAATTAACGGAATCATGTTCATACGAGCTCCCaa        >  1:1068967/1‑150 (MQ=255)
                                                                                                                                               aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTACCAAt       >  1:1776209/1‑150 (MQ=255)
                                                                                                                                                  gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCTGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCACAAtttt    >  2:1275403/1‑150 (MQ=255)
                                                                                                                                                   gttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGATATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg   <  2:685460/149‑1 (MQ=255)
                                                                                                                                                   cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGATATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCTATTTTg   <  1:1427701/150‑1 (MQ=255)
                                                                                                                                                    ttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAGATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTgg  <  1:1444466/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCACCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGG  >  NC_000913/2270454‑2270751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: