Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,270,605 G→A 5.5% intergenic (+60/‑121) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,270,6050GA 5.5% 453.8 / 10.2 146intergenic (+60/‑121)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (70/68);  new base A (6/2);  total (76/70)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

Only 100 of 148 total aligned reads displayed.

CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAC  >  NC_000913/2270457‑2270753
                                                                                                                                                    |                                                                                                                                                    
ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg                                                                                                                                                     <  1:1613196/150‑1 (MQ=255)
ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg                                                                                                                                                     >  2:1995522/1‑150 (MQ=255)
 cagcagTGGTGTTATAATTTCCAGCATTCATGAACCTTACTGGAAGAAGCGTTAAAACGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGAAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGc                                                                                                                                                    <  2:591234/150‑1 (MQ=255)
  agcagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCa                                                                                                                                                   <  1:45901/150‑1 (MQ=255)
   gcagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAg                                                                                                                                                  <  1:868379/150‑1 (MQ=255)
      gtggtgGTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTGGGCGATCCTGACGAGTTAACTGAAAGCACGGCTTAGGCAGTGc                                                                                                                                               >  1:393330/1‑150 (MQ=255)
          tgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttt                                                                                                                                           >  2:938451/1‑150 (MQ=255)
          tgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttt                                                                                                                                           <  2:570269/150‑1 (MQ=255)
           gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCATTGCttttt                                                                                                                                          >  1:994186/1‑150 (MQ=255)
           gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                          >  1:700025/1‑150 (MQ=255)
           gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCttttt                                                                                                                                          <  2:1424871/150‑1 (MQ=255)
           gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttca                                                                                                                                          <  1:16608/150‑3 (MQ=255)
            ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTTTGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttcag                                                                                                                                         <  2:1416452/150‑4 (MQ=255)
             tattatTTCCAGCATGAATGAACCGTACTGGAAGACGCGGTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                        <  1:1043284/150‑1 (MQ=255)
             tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTATTAAACCGTTTGGATTCAATCCCTTGGCTATCCTGACGAGTTAACTGATAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                        <  1:503685/150‑1 (MQ=255)
             tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGTATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                        <  2:1061160/150‑1 (MQ=255)
              attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGt                                                                                                                                       >  1:1382096/1‑150 (MQ=255)
               ttattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtt                                                                                                                                      <  1:456635/150‑1 (MQ=255)
                 attTCCAGCATGAATGAACCGTACGGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtttt                                                                                                                                    >  1:1007791/1‑150 (MQ=255)
                  tttCCAGCATGAATGAACCGTACTGGAAGAATCGTTACATCGAAGCACGCCGGGTTCTCAGCAGCAGCTAATAAACCGTTTGGTTGCAATCCCTTGGCTTTCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttc                                                                                                                                   <  1:1942859/150‑1 (MQ=255)
                   ttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttca                                                                                                                                  <  1:1687110/150‑1 (MQ=255)
                    tCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcat                                                                                                                                 <  2:88331/150‑1 (MQ=255)
                     ccAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTGAGGCAGTGCTTTGTTGTTttcatt                                                                                                                                >  1:210718/1‑150 (MQ=255)
                       aGCATGAAGGACCCGTACTGGACGAAGCGTGACAACGAAGCACCCCGGGCTCTCAGCCGCAGCTAATAAACCGTGTGGATGCAATCCCTAGGCTATCCTGACGAGTTCACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcattca                                                                                                                              <  2:1425667/150‑1 (MQ=255)
                        gCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTtcattcat                                                                                                                             >  1:1554648/1‑150 (MQ=255)
                         cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                            <  1:1213336/150‑1 (MQ=255)
                         cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                            <  1:1729299/150‑1 (MQ=255)
                         cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCGTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc                                                                                                                            >  2:343677/1‑150 (MQ=255)
                          atgaatgaACCGTCCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTATGGATGCAATCCCAAGGCTATCCTGACGAGTTTCCTGAAAGCACTGCTGAGGCAGTGCGTGTTTGTTGTCATtcatca                                                                                                                           >  1:317581/1‑150 (MQ=255)
                               tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaa                                                                                                                      >  1:631255/1‑150 (MQ=255)
                                      tACTGCAAGAAGCGGTTCAACGAACCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTTTCCTGACGAGTTAACTGAAAGCTCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAatgatg                                                                                                               <  2:1667288/150‑1 (MQ=255)
                                          ggcagaagCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                           <  2:806099/147‑1 (MQ=255)
                                          ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCCGCTTATAAGCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCGTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                           >  2:1195187/1‑150 (MQ=255)
                                          ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                           >  2:1117280/1‑150 (MQ=255)
                                          ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTc                                                                                                           >  1:819893/1‑150 (MQ=255)
                                            aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                         <  1:137339/150‑1 (MQ=255)
                                            aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                         <  1:1564660/150‑1 (MQ=255)
                                            aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATACTGACGAGTTAAATGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                         >  1:1527224/1‑150 (MQ=255)
                                             agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGGTTTTTTGTTTTCATTATTCAGAGAAATTGATGTTTCagc                                                                                                        >  2:1897014/1‑150 (MQ=255)
                                             agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCTTCAGAGAAAATGATGTTTCcgg                                                                                                        >  2:103753/1‑149 (MQ=255)
                                              gaagCGTTACACCGAAGCACGCCGGTTTCTCAGCCGCAGCTAAAAAACCGTTTGGATGCAAGCCCTTGGCTATCCTGACGCGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTTTTTCATTCATCAGAGAAAATGATGTTTCcgcg                                                                                                       <  2:1341733/150‑1 (MQ=255)
                                                  cGTTACCACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAACGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATTATGTTTCCGCGTCtt                                                                                                   <  2:1247332/150‑1 (MQ=255)
                                                  cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                                                                   <  1:1814125/150‑1 (MQ=255)
                                                  cGTTACAACGAAGCACGCCGGGTTCTCAGCAGCAGCTAATAAAACGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt                                                                                                   <  2:515768/150‑1 (MQ=255)
                                                   gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTg                                                                                                  >  1:505291/1‑150 (MQ=255)
                                                        aaCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCa                                                                                             >  2:536763/1‑150 (MQ=255)
                                                         aCGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAg                                                                                            <  1:1258480/150‑1 (MQ=255)
                                                                aCGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAg                                                                                     <  1:536763/150‑1 (MQ=255)
                                                                 cGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGt                                                                                    >  1:85026/1‑150 (MQ=255)
                                                                   ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGATTAGGCAGTGCTTTTTTGTTTACATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATACAGGGTATAGTcc                                                                                  >  2:1016870/1‑150 (MQ=255)
                                                                     gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGATATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg                                                                                <  1:1345370/150‑1 (MQ=255)
                                                                          ctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt                                                                           >  2:1074070/1‑150 (MQ=255)
                                                                           tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTg                                                                          <  1:679100/150‑1 (MQ=255)
                                                                            cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGt                                                                         <  2:1032252/150‑1 (MQ=255)
                                                                              gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTTCTTTTTTGTTTTCATTCATCAGAGAAATTGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                                                                       <  1:1035422/150‑1 (MQ=255)
                                                                              gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTa                                                                       >  2:185624/1‑150 (MQ=255)
                                                                                cGCTGCTAATAAACCGGTTGGATGCAATCCCTAGGCAATCCTGACGAGTTAACTGACAGGACTGCTTAGGCAGTGCTTTTTTGTTTTCATCCATCAGAGAACATGAGGTTTCCGCGTCTTGATCCCGGCTATAGTCCGGGCATTGTTATc                                                                     <  2:295690/150‑1 (MQ=255)
                                                                                               gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCAAGGCTATAGTCCGGTCATTGTTATCTTTTTAATGTTTTct                                                      >  1:387759/1‑149 (MQ=255)
                                                                                                  tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAAAGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATTTTGTCGTaa                                                   >  2:114356/1‑150 (MQ=255)
                                                                                                    gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCGTTTAAATGTTGTCGTAAtt                                                 >  2:809267/1‑150 (MQ=255)
                                                                                                      tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATGAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTc                                               <  2:1505478/150‑1 (MQ=255)
                                                                                                       gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa                                              <  1:1991429/150‑1 (MQ=255)
                                                                                                        cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                             >  2:600313/1‑150 (MQ=255)
                                                                                                        cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                             >  2:1907467/1‑150 (MQ=255)
                                                                                                        cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                             >  1:1320783/1‑150 (MQ=255)
                                                                                                          aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCTCGTCTTGATCCAGGATATAGTCCGGTCATTGTTATCTTTTAAATGTTTTCGTAATTTCAGGa                                           >  1:1611078/1‑150 (MQ=255)
                                                                                                           tCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATAGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa                                          >  1:1253792/1‑150 (MQ=255)
                                                                                                             ccTTGGCTATCCTTACGAGATAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTATCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                        <  1:338366/150‑1 (MQ=255)
                                                                                                             ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                        <  2:1210641/150‑1 (MQ=255)
                                                                                                                 ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc                                    >  2:1301402/1‑150 (MQ=255)
                                                                                                                 ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc                                    <  2:1382095/150‑1 (MQ=255)
                                                                                                                  gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg                                   <  1:1221055/150‑1 (MQ=255)
                                                                                                                       ccTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCCGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATAAACGGAAta                              >  2:1533734/1‑149 (MQ=255)
                                                                                                                       ccTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATc                              >  2:905960/1‑150 (MQ=255)
                                                                                                                        cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                             >  2:1370395/1‑150 (MQ=255)
                                                                                                                          gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCAGCGTCTTGGTCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATg                           >  2:1470808/1‑150 (MQ=255)
                                                                                                                            cGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                         >  1:1124703/1‑150 (MQ=255)
                                                                                                                             gAGTTTACTGTAAGCTCTGCTTAGTCTGTGTTTTTTTTTTTTCATTCATCTGAGAAAATCATGTTTCAGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATTTTc                        <  2:1245436/150‑1 (MQ=255)
                                                                                                                              aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                       <  2:85026/150‑1 (MQ=255)
                                                                                                                              aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                       <  2:1413592/150‑1 (MQ=255)
                                                                                                                               gTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                      >  2:454917/1‑150 (MQ=255)
                                                                                                                                ttAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATa                     <  2:700024/150‑1 (MQ=255)
                                                                                                                                aaacaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATa                     >  2:572103/8‑150 (MQ=255)
                                                                                                                                 tAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                    >  1:1844877/1‑150 (MQ=255)
                                                                                                                                  aaCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcg                   >  1:267574/1‑150 (MQ=255)
                                                                                                                                    cTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGTCTATAGTCCGGTCATTGTGATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgcc                 >  2:1793342/1‑149 (MQ=255)
                                                                                                                                     aaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                >  2:1610103/3‑150 (MQ=255)
                                                                                                                                       aaaGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGGCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgcgc              <  1:2015278/150‑3 (MQ=255)
                                                                                                                                        aaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCGTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAGCATGTTCATACGCGCTcc             >  2:304166/1‑150 (MQ=255)
                                                                                                                                         aGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATACAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAAGCGCTcgc            >  1:148381/1‑148 (MQ=255)
                                                                                                                                           cACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATATTATAAATGTTGTCGTAATTTCAGTAAATTAACGGAATCATGTTCATACGAGCTCCCaa          >  1:1068967/1‑150 (MQ=255)
                                                                                                                                            aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTACCAAt         >  1:1776209/1‑150 (MQ=255)
                                                                                                                                               gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt      >  2:1743392/1‑150 (MQ=255)
                                                                                                                                                gttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGATATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg     <  2:685460/149‑1 (MQ=255)
                                                                                                                                                cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGATATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCTATTTTg     <  1:1427701/150‑1 (MQ=255)
                                                                                                                                                 ttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAGATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTgg    <  1:1444466/150‑1 (MQ=255)
                                                                                                                                                   aGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc  <  2:1798662/150‑1 (MQ=255)
                                                                                                                                                   aGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc  <  2:1554647/150‑1 (MQ=255)
                                                                                                                                                   aGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc  <  1:1995523/150‑1 (MQ=255)
                                                                                                                                                   aGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc  <  1:27800/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAC  >  NC_000913/2270457‑2270753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: