Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,142 T→A 7.3% intergenic (+6/‑69) yejM → / → proL essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,1420TA 7.3% 306.2 / 10.9 116intergenic (+6/‑69)yejM/proLessential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (48/59);  new base A (5/4);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 132 total aligned reads displayed.

ACCCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAA  >  NC_000913/2285993‑2286289
                                                                                                                                                     |                                                                                                                                                   
accCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGattaatt                                                                                                                                                     <  2:774160/150‑1 (MQ=255)
accCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGattaatt                                                                                                                                                     >  1:1712459/1‑150 (MQ=255)
 ccccGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGattaatta                                                                                                                                                    >  1:434645/1‑150 (MQ=255)
  cccGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATtat                                                                                                                                                   >  1:1315724/1‑150 (MQ=255)
  cccGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATtat                                                                                                                                                   <  2:1998578/150‑1 (MQ=255)
  cccGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAAATGATTAATtat                                                                                                                                                   >  1:918597/1‑150 (MQ=255)
   ccGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATtata                                                                                                                                                  >  1:697081/1‑150 (MQ=255)
     gAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa                                                                                                                                                <  2:58101/150‑1 (MQ=255)
        aaaGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCa                                                                                                                                             <  2:840329/150‑1 (MQ=255)
             cGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAg                                                                                                                                        <  1:833273/150‑1 (MQ=255)
             cGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg                                                                                                                                        <  2:238846/150‑1 (MQ=255)
              gCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAGc                                                                                                                                       <  1:744749/150‑1 (MQ=255)
               cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAACCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCg                                                                                                                                      >  2:36784/1‑150 (MQ=255)
               cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAGCg                                                                                                                                      <  1:398385/150‑1 (MQ=255)
                tGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGa                                                                                                                                     <  2:582045/150‑1 (MQ=255)
                  gTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGaaa                                                                                                                                   <  1:1886490/150‑1 (MQ=255)
                    aCTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata                                                                                                                                 >  2:1877258/1‑150 (MQ=255)
                    aCTGAACAAAAACGGTAAATACCGCACTTACAACTTACGGGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata                                                                                                                                 <  1:1829643/150‑1 (MQ=255)
                       gAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAATTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCt                                                                                                                              >  1:1016553/1‑150 (MQ=255)
                       gAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCt                                                                                                                              >  1:489659/1‑150 (MQ=255)
                         aCAATAACGGTAAATACCGCACTTACAACTTTCGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTa                                                                                                                            >  2:1595182/1‑150 (MQ=255)
                         aCAATAACGGTAAATACCGCACTTACAACTTACGTGTTGTAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTTTTGCTAGTTCTGACAGACGTGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCttt                                                                                                                            <  1:958348/150‑2 (MQ=255)
                         aCAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTAATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTa                                                                                                                            <  1:1064243/150‑1 (MQ=255)
                           aaTAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGCGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACt                                                                                                                          <  2:1746776/150‑1 (MQ=255)
                             tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:911358/150‑1 (MQ=255)
                             tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:484551/150‑1 (MQ=255)
                             tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:1013460/150‑1 (MQ=255)
                              aaCGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCAAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                       <  1:1015212/150‑1 (MQ=255)
                              aaCGCTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAATATTAAAAACCACAGTTAAGTTTGTTATTGCAAGTCCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                       <  1:329639/150‑1 (MQ=255)
                                    aaaTACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCgg                                                                                                                 <  2:512010/150‑1 (MQ=255)
                                       tACCGCACATACAACTTACGTGGTGAAAGAGTGAAAGATGAAAATCCTCAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCTTTTTATCGCTAACTGAATAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgtgt                                                                                                              <  1:1803478/150‑1 (MQ=255)
                                        aCCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                             <  2:1679080/150‑1 (MQ=255)
                                        aCCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                             >  1:1088652/1‑150 (MQ=255)
                                          cGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACACTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATAACATACTTGAAAACTGTGTGGa                                                                                                           >  2:975379/1‑150 (MQ=255)
                                              cTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAAc                                                                                                       >  1:177565/1‑150 (MQ=255)
                                                tACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACgg                                                                                                     >  2:1271252/1‑150 (MQ=255)
                                                 aCAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt                                                                                                    >  2:1077352/1‑150 (MQ=255)
                                                 aCAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt                                                                                                    <  2:1363846/150‑1 (MQ=255)
                                                  cAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTTCAATCGGTGTGGAAAACGgta                                                                                                   >  2:1055544/1‑150 (MQ=255)
                                                   aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTCAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                  <  2:480397/150‑1 (MQ=255)
                                                           tggtgAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAATTCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagca                                                                                          >  1:1419092/1‑150 (MQ=255)
                                                             gtgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcagc                                                                                        <  2:1398385/150‑1 (MQ=255)
                                                              ttaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc                                                                                       <  2:483467/148‑1 (MQ=255)
                                                              tgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc                                                                                       <  2:696872/150‑1 (MQ=255)
                                                                 aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    <  2:568847/150‑1 (MQ=255)
                                                                 aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    >  1:1561307/1‑150 (MQ=255)
                                                                 aagagcgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCTAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    >  1:959452/1‑150 (MQ=255)
                                                                  agagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa                                                                                   <  1:1337047/150‑1 (MQ=255)
                                                                  agagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa                                                                                   >  2:1390061/1‑150 (MQ=255)
                                                                     gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                <  2:1251863/150‑1 (MQ=255)
                                                                     gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                >  2:409314/1‑150 (MQ=255)
                                                                     gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                >  2:1210452/1‑150 (MQ=255)
                                                                      tgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCg                                                                               <  1:539097/150‑1 (MQ=255)
                                                                       gaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAAAGGTAGTATTAGCATCCACGATTCgg                                                                              >  1:268975/1‑150 (MQ=255)
                                                                         aagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGAGAAATATCTTACTTGCAATCGGAGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCa                                                                            >  1:2050241/1‑150 (MQ=255)
                                                                          agaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGTGAAGCGTTTTATCGCTAACTGATTAATTATAAATAAGTTAGCGATATATCTTACTTGCAATCGGTTTGGAAAACGGTAGTATTAGCAGCAACGAGTCGGCAc                                                                           >  1:277235/1‑150 (MQ=255)
                                                                           gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATTATAAATCAGTTAGCGAAATATCTTACATGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACg                                                                          <  2:361060/150‑1 (MQ=255)
                                                                           gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACg                                                                          <  1:1742779/150‑1 (MQ=255)
                                                                             tGAAAAACCACAGTTAAGTTTGTTATTGCTAGTGCTGACAGACTAGAAGCGTTTTATCGCTAACTGATTAATTATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTa                                                                        <  1:1548330/150‑1 (MQ=255)
                                                                               aaaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTGACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgc                                                                      >  1:80589/1‑150 (MQ=255)
                                                                                 aaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgcgc                                                                    <  1:560872/150‑1 (MQ=255)
                                                                                  aaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCa                                                                   >  1:1270022/1‑150 (MQ=255)
                                                                                       caGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCTCTAACTGATAAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTg                                                              <  2:141966/150‑1 (MQ=255)
                                                                                       caGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTg                                                              <  2:691004/150‑1 (MQ=255)
                                                                                        aGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAAATATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTgg                                                             <  1:343467/150‑1 (MQ=255)
                                                                                                ttGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCAc                                                     <  1:206755/150‑1 (MQ=255)
                                                                                                  gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCg                                                   <  2:636361/150‑1 (MQ=255)
                                                                                                  gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCg                                                   <  1:684899/150‑1 (MQ=255)
                                                                                                       tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCTCACCGTCATg                                              >  1:58818/1‑150 (MQ=255)
                                                                                                         cAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATggg                                            >  1:811734/1‑150 (MQ=255)
                                                                                                          aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgggg                                           <  2:1280163/150‑1 (MQ=255)
                                                                                                          aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgggg                                           <  1:1474530/150‑1 (MQ=255)
                                                                                                               cTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCg                                      >  2:420638/1‑150 (MQ=255)
                                                                                                                 gACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggg                                    >  2:1108676/1‑150 (MQ=255)
                                                                                                                   cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggggg                                  >  2:1749619/1‑150 (MQ=255)
                                                                                                                     gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCCGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTc                                >  1:992955/1‑150 (MQ=255)
                                                                                                                     gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTc                                >  1:1759378/1‑150 (MQ=255)
                                                                                                                      aCGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCg                               >  1:1004730/1‑150 (MQ=255)
                                                                                                                        gagaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGa                             >  2:1087174/1‑150 (MQ=255)
                                                                                                                         cgaAGCGGTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTATTTGCAATCGGTGTGGAAACCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAg                            <  2:1237979/149‑1 (MQ=255)
                                                                                                                          gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAgg                           >  1:212524/1‑150 (MQ=255)
                                                                                                                          gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAgg                           <  2:943769/150‑1 (MQ=255)
                                                                                                                           aaGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGt                          <  1:1797232/150‑1 (MQ=255)
                                                                                                                            cgCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGtt                         <  2:1190368/149‑1 (MQ=255)
                                                                                                                                 tttATCGCTTAATTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAt                    <  2:434645/150‑1 (MQ=255)
                                                                                                                                 tttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAAAATCTTACTTGCAATCGGGGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAt                    <  2:427139/150‑1 (MQ=255)
                                                                                                                                   tttCGCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATcc                  >  2:26976/3‑150 (MQ=255)
                                                                                                                                       gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  1:1444672/1‑150 (MQ=255)
                                                                                                                                       gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  1:2040164/1‑150 (MQ=255)
                                                                                                                                       gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  2:154660/1‑150 (MQ=255)
                                                                                                                                       gCTAACTGATTTATAATTAAGCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGAATCCTGGTCGCGCACCGTAATGGGGTGTCGGGGGTCGGAGGTGCAAATcgagtc              >  1:1305941/1‑145 (MQ=255)
                                                                                                                                         tAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGt            >  2:985670/1‑150 (MQ=255)
                                                                                                                                          aaCTGATTAATTATACATCAGTTAGCGAAATATCTTACTTGCAATCGGTGGGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTg           <  1:1934141/150‑1 (MQ=255)
                                                                                                                                            cTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGcc         >  1:958562/1‑150 (MQ=255)
                                                                                                                                             tGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCg        >  1:915249/1‑150 (MQ=255)
                                                                                                                                              gATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGa       >  1:1605886/1‑150 (MQ=255)
                                                                                                                                               attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAc      <  2:1708928/150‑1 (MQ=255)
                                                                                                                                               attaataaTAAATCAGTTAGCGTAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAc      <  2:754336/150‑1 (MQ=255)
                                                                                                                                                 taattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCAGGGGTCGGAGGGTCAAATCCTCTCGTGCCGACCa    <  2:1448558/150‑1 (MQ=255)
                                                                                                                                                   attaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGGCGACCaaa  <  1:2028804/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACCCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAA  >  NC_000913/2285993‑2286289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: