Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,145 A→T 7.1% intergenic (+9/‑66) yejM → / → proL essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,1450AT 7.1% 324.6 / 10.4 119intergenic (+9/‑66)yejM/proLessential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (48/62);  new base T (5/4);  total (53/66)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

Only 100 of 122 total aligned reads displayed.

CCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAA  >  NC_000913/2285996‑2286289
                                                                                                                                                     |                                                                                                                                                
  gAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa                                                                                                                                                <  2:58101/150‑1 (MQ=255)
          cGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAg                                                                                                                                        <  1:833273/150‑1 (MQ=255)
          cGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg                                                                                                                                        <  2:238846/150‑1 (MQ=255)
           gCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAGc                                                                                                                                       <  1:744749/150‑1 (MQ=255)
            cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAACCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCg                                                                                                                                      >  2:36784/1‑150 (MQ=255)
            cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAGCg                                                                                                                                      <  1:398385/150‑1 (MQ=255)
            cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGAGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCg                                                                                                                                      >  1:1812596/1‑150 (MQ=255)
                tACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAat                                                                                                                                  <  2:1220728/150‑1 (MQ=255)
                 aCTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata                                                                                                                                 >  2:1877258/1‑150 (MQ=255)
                 aCTGAACAAAAACGGTAAATACCGCACTTACAACTTACGGGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata                                                                                                                                 <  1:1829643/150‑1 (MQ=255)
                    gAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAATTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCt                                                                                                                              >  1:1016553/1‑150 (MQ=255)
                    gAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCt                                                                                                                              >  1:489659/1‑150 (MQ=255)
                      aCAATAACGGTAAATACCGCACTTACAACTTTCGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTa                                                                                                                            >  2:1595182/1‑150 (MQ=255)
                      aCAATAACGGTAAATACCGCACTTACAACTTACGTGTTGTAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTTTTGCTAGTTCTGACAGACGTGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCttt                                                                                                                            <  1:958348/150‑2 (MQ=255)
                       cAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTaa                                                                                                                           >  1:753824/1‑149 (MQ=255)
                       cAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTAc                                                                                                                           >  1:1275001/1‑150 (MQ=255)
                        aaTAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGCGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACt                                                                                                                          <  2:1746776/150‑1 (MQ=255)
                          tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:1013460/150‑1 (MQ=255)
                          tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:484551/150‑1 (MQ=255)
                          tAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTg                                                                                                                        <  1:911358/150‑1 (MQ=255)
                           aaCGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCAAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                       <  1:1015212/150‑1 (MQ=255)
                           aaCGCTAAATACCGCACATACAACTTACGTGGTGAAAGAGTGAACGAGGAAAAACCACACATAAGTTTGTTATTGCAAGTGCTGACAGACGAGACGCGTTTTATCGCTAACTGATTAATTATAAATCAGGTAGCGAAATATCTTACTTGc                                                                                                                       <  2:302756/150‑1 (MQ=255)
                                 aaaTACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCgg                                                                                                                 <  2:512010/150‑1 (MQ=255)
                                    tACCGCACATACAACTTACGTGGTGAAAGAGTGAAAGATGAAAATCCTCAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCTTTTTATCGCTAACTGAATAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgtgt                                                                                                              <  1:1803478/150‑1 (MQ=255)
                                     aCCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                             <  2:1679080/150‑1 (MQ=255)
                                     aCCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                             >  1:1088652/1‑150 (MQ=255)
                                       cGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACACTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATAACATACTTGAAAACTGTGTGGa                                                                                                           >  2:975379/1‑150 (MQ=255)
                                             tACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACgg                                                                                                     >  2:1271252/1‑150 (MQ=255)
                                              aCAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt                                                                                                    >  2:1077352/1‑150 (MQ=255)
                                               cAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTTCAATCGGTGTGGAAAACGgta                                                                                                   >  2:1055544/1‑150 (MQ=255)
                                                aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTCAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                  <  2:480397/150‑1 (MQ=255)
                                                        tggtgAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAATTCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagca                                                                                          >  1:1419092/1‑150 (MQ=255)
                                                          gtgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcagc                                                                                        <  2:1398385/150‑1 (MQ=255)
                                                           tgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc                                                                                       <  2:696872/150‑1 (MQ=255)
                                                              aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGATAACTGTAGTATTAGCAGCCACg                                                                                    >  2:1864911/1‑150 (MQ=255)
                                                              aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    <  1:591023/150‑1 (MQ=255)
                                                              aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    <  2:168829/150‑1 (MQ=255)
                                                              aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    <  2:568847/150‑1 (MQ=255)
                                                              aagagcgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCTAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                    >  1:959452/1‑150 (MQ=255)
                                                               agagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa                                                                                   <  1:1337047/150‑1 (MQ=255)
                                                               agagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa                                                                                   >  2:1390061/1‑150 (MQ=255)
                                                                  gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                >  2:1210452/1‑150 (MQ=255)
                                                                  gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                <  2:1251863/150‑1 (MQ=255)
                                                                  gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                >  1:938463/1‑150 (MQ=255)
                                                                  gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                                >  2:409314/1‑150 (MQ=255)
                                                                   tgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCg                                                                               <  2:1020961/150‑1 (MQ=255)
                                                                    gaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAAAGGTAGTATTAGCATCCACGATTCgg                                                                              >  1:268975/1‑150 (MQ=255)
                                                                     aaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGc                                                                             <  1:1023221/150‑1 (MQ=255)
                                                                      aagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGAGAAATATCTTACTTGCAATCGGAGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCa                                                                            >  1:2050241/1‑150 (MQ=255)
                                                                       agaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGTGAAGCGTTTTATCGCTAACTGATTAATTATAAATAAGTTAGCGATATATCTTACTTGCAATCGGTTTGGAAAACGGTAGTATTAGCAGCAACGAGTCGGCAc                                                                           >  1:277235/1‑150 (MQ=255)
                                                                        gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATTATAAATCAGTTAGCGAAATATCTTACATGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACg                                                                          <  2:361060/150‑1 (MQ=255)
                                                                        gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACg                                                                          <  1:1742779/150‑1 (MQ=255)
                                                                          tGAAAAACCACAGTTAAGTTTGTTATTGCTAGTGCTGACAGACTAGAAGCGTTTTATCGCTAACTGATTAATTATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTa                                                                        <  1:1548330/150‑1 (MQ=255)
                                                                            aaaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTGACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgc                                                                      >  1:80589/1‑150 (MQ=255)
                                                                             aaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgcg                                                                     >  1:534922/1‑150 (MQ=255)
                                                                              aaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgcgc                                                                    <  1:560872/150‑1 (MQ=255)
                                                                               aaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCa                                                                   >  1:1270022/1‑150 (MQ=255)
                                                                               aaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATATTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCTGCACGTAGCGCa                                                                   >  2:55535/1‑150 (MQ=255)
                                                                                    caGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCTCTAACTGATAAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTg                                                              <  2:141966/150‑1 (MQ=255)
                                                                                    caGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTg                                                              <  2:691004/150‑1 (MQ=255)
                                                                                     aGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAAATATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTgg                                                             <  1:343467/150‑1 (MQ=255)
                                                                                      gctAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTGTTATCGCTAACTGATTACTAATAAATCAGGTAGCGAAATATCTTACTAGCAATCGGGGGGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGt                                                            <  1:166323/148‑1 (MQ=255)
                                                                                             ttGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCAc                                                     <  1:206755/150‑1 (MQ=255)
                                                                                               gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCg                                                   <  1:684899/150‑1 (MQ=255)
                                                                                               gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCg                                                   <  2:636361/150‑1 (MQ=255)
                                                                                                ttATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTTGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGt                                                  >  2:448512/1‑150 (MQ=255)
                                                                                                    tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCTCACCGTCATg                                              >  1:58818/1‑150 (MQ=255)
                                                                                                      cAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATggg                                            >  1:811734/1‑150 (MQ=255)
                                                                                                       aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgggg                                           <  2:1280163/150‑1 (MQ=255)
                                                                                                       aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgggg                                           <  1:1474530/150‑1 (MQ=255)
                                                                                                            cTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCg                                      >  2:420638/1‑150 (MQ=255)
                                                                                                              gACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggg                                    >  2:1108676/1‑150 (MQ=255)
                                                                                                                cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggggg                                  >  2:1749619/1‑150 (MQ=255)
                                                                                                                  gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCCGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTc                                >  1:992955/1‑150 (MQ=255)
                                                                                                                  gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTc                                >  1:1759378/1‑150 (MQ=255)
                                                                                                                   aCGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCg                               >  1:1004730/1‑150 (MQ=255)
                                                                                                                       gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAgg                           >  1:212524/1‑150 (MQ=255)
                                                                                                                       gaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAgg                           <  2:943769/150‑1 (MQ=255)
                                                                                                                        aaGCTTTTTATCGCTAACTTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGt                          <  2:918596/150‑1 (MQ=255)
                                                                                                                        aaGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGt                          <  1:1797232/150‑1 (MQ=255)
                                                                                                                         cgCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGtt                         <  2:1190368/149‑1 (MQ=255)
                                                                                                                         aGCGTTGTATCGCTAACTGATTAAGTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAACACGGGAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGtt                         <  1:306540/150‑1 (MQ=255)
                                                                                                                          gCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTc                        <  2:1088651/150‑1 (MQ=255)
                                                                                                                           cGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCa                       <  1:1760081/150‑1 (MQ=255)
                                                                                                                              tttATCGCTTAATTATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAt                    <  2:434645/150‑1 (MQ=255)
                                                                                                                              tttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAAAATCTTACTTGCAATCGGGGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAt                    <  2:427139/150‑1 (MQ=255)
                                                                                                                                 aTCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCct                 <  2:1275000/150‑1 (MQ=255)
                                                                                                                                  tCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCctc                <  2:1391444/150‑1 (MQ=255)
                                                                                                                                    gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  2:154660/1‑150 (MQ=255)
                                                                                                                                    gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  1:1444672/1‑150 (MQ=255)
                                                                                                                                    gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc              >  1:2040164/1‑150 (MQ=255)
                                                                                                                                    gCTAACTGATTTATAATTAAGCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGAATCCTGGTCGCGCACCGTAATGGGGTGTCGGGGGTCGGAGGTGCAAATcgagtc              >  1:1305941/1‑145 (MQ=255)
                                                                                                                                      tAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGt            >  2:985670/1‑150 (MQ=255)
                                                                                                                                       aaCTGATTAATTATACATCAGTTAGCGAAATATCTTACTTGCAATCGGTGGGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTg           <  1:1934141/150‑1 (MQ=255)
                                                                                                                                          tGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCg        >  1:915249/1‑150 (MQ=255)
                                                                                                                                           gATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGa       >  1:1605886/1‑150 (MQ=255)
                                                                                                                                            attaataaTAAATCAGTTAGCGTAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAc      <  2:754336/150‑1 (MQ=255)
                                                                                                                                             ttaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAcc     >  2:703478/1‑150 (MQ=255)
                                                                                                                                              taattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCAGGGGTCGGAGGGTCAAATCCTCTCGTGCCGACCa    <  2:1448558/150‑1 (MQ=255)
                                                                                                                                                attaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGGCGACCaaa  <  1:2028804/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAA  >  NC_000913/2285996‑2286289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: