Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,357,653 A→G 7.0% L250L (CTA→CTG)  yfaD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,357,6530AG 7.0% 268.5 / 5.0 100L250L (CTA→CTG) yfaDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base A (40/51);  new base G (3/4);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATT  >  NC_000913/2357505‑2357801
                                                                                                                                                    |                                                                                                                                                    
gagGCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACAACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTAc                                                                                                                                                     >  1:1383961/1‑150 (MQ=255)
  ggCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACgg                                                                                                                                                   >  1:664312/1‑150 (MQ=255)
  ggCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACgg                                                                                                                                                   >  2:1351312/1‑150 (MQ=255)
  ggCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTATCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACgg                                                                                                                                                   >  1:1216512/1‑150 (MQ=255)
   gCTTCACTAATGACATCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGc                                                                                                                                                  <  1:789077/150‑1 (MQ=255)
   gCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGc                                                                                                                                                  >  1:642680/1‑150 (MQ=255)
    cTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCa                                                                                                                                                 <  1:78505/150‑1 (MQ=255)
         cTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAg                                                                                                                                            >  2:451252/1‑150 (MQ=255)
         cTAATGAAAGCCAGCTACAAACTATGTTTAATTCTCTGCTGCAATGCGGCGATACCTCCCGTTTCACCGGTTTTATTGAGGAGATTGCGGAACGTTCACCACTACAAAAGGAGAGTTTTATGACTATTGCTGAACGGCTACGGCAGGAAg                                                                                                                                            <  1:1923278/150‑1 (MQ=38)
           aaTGTCAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAggg                                                                                                                                          >  2:500103/1‑150 (MQ=255)
           aaTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAggg                                                                                                                                          <  1:933027/150‑1 (MQ=255)
             tGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCa                                                                                                                                        <  2:1600517/150‑1 (MQ=255)
             tGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCa                                                                                                                                        <  2:28063/150‑1 (MQ=255)
               aCAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTACCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATc                                                                                                                                      >  2:973817/1‑150 (MQ=255)
                cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCa                                                                                                                                     >  1:958597/1‑150 (MQ=255)
                cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCa                                                                                                                                     <  1:1747629/150‑1 (MQ=255)
                cagccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGACATCa                                                                                                                                     >  1:154267/1‑150 (MQ=255)
                  gccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCaaa                                                                                                                                   >  1:759928/1‑150 (MQ=255)
                  gccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCACCCGTTTAAAACGTTTTATTGAGGAGATTGCCGAACGTTACCCACTGCAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCaaa                                                                                                                                   >  2:310581/1‑150 (MQ=38)
                     agcTACAAACACTGTTTAATTATCTGCTGCACTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtg                                                                                                                                <  1:590561/150‑1 (MQ=255)
                     agcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtg                                                                                                                                <  1:623702/150‑1 (MQ=255)
                     agcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGATTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtg                                                                                                                                <  1:664178/150‑1 (MQ=255)
                        tACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGATATTGCCGAACGTTCACCACTACAATAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggct                                                                                                                             >  1:385901/1‑150 (MQ=255)
                        tACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTACACCCGTGTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCAGCAAATtggct                                                                                                                             <  2:316465/150‑1 (MQ=255)
                          cAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGGCATCAAAAtggctgg                                                                                                                           >  2:329898/1‑150 (MQ=255)
                          cAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctgg                                                                                                                           >  1:1272881/1‑150 (MQ=255)
                          cAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTACACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctgg                                                                                                                           >  2:1259037/1‑150 (MQ=255)
                           aaaCACTGTTTAATTATCTGCTGCAATGCGGCGATAACTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctggc                                                                                                                          <  2:1157806/150‑1 (MQ=255)
                            aaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCa                                                                                                                         >  1:1933879/1‑150 (MQ=255)
                            aaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCa                                                                                                                         <  1:1612837/150‑1 (MQ=255)
                            aaCACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTATATTGAGGAGATTGACGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGCCTACGGCAGGAAGCGCATCAAATTGCCTGGCa                                                                                                                         >  2:599911/1‑150 (MQ=38)
                             agacTGTTTAATTATCTGCTGCAATGAGGCGATAATTCGCGTTTGACACGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAg                                                                                                                        <  2:154267/148‑1 (MQ=25)
                             acacTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAg                                                                                                                        <  2:766363/150‑1 (MQ=255)
                                 tGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCCCCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAg                                                                                                                    >  2:1394054/1‑150 (MQ=255)
                                  gTTTAATTATCTGCTGCTATGAGGCGATACCTCCCGTTTCACCAGTGTTATTTAGGAGATTGCCCAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAgg                                                                                                                   <  2:642680/150‑1 (MQ=21)
                                  gTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCAGCAAATTGGCTGGCAGGAAgg                                                                                                                   <  1:1374601/150‑1 (MQ=255)
                                    ttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAATGAGAGATTAATGACTATTGCTGAACGGGTACGGCAGGAAGGGCATCATATTGGCTGGCAGGAAGGTa                                                                                                                 <  2:855467/150‑1 (MQ=255)
                                      aaTTATCTGCTCGATTGCGGCGATAGCTCCCGTTTCACCAGTTTTATTGAGGATTTTGCCGAACGTTCACCACTATAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaa                                                                                                               <  1:329898/150‑3 (MQ=21)
                                         tttCTGCTTCAATGCGGCGTTACCTCCCGTTTCACCGGTTTTATTGAGGAGATTGCCGTACGTTCACCACTACTAAAGGAGATATTAATGACTAATGCTGAACGGCTACGGCTGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt                                                                                                            <  1:1380058/148‑1 (MQ=25)
                                         tATCTGCTGCAATCCGGCGATACCTCCCGTGCGACCCGCCTACTTGAGGAGCCTGCCCAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatta                                                                                                            <  2:1416231/150‑6 (MQ=17)
                                          aTCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAATGATAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAtt                                                                                                           >  2:1385638/1‑149 (MQ=255)
                                           tCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGa                                                                                                          <  2:1585799/150‑1 (MQ=255)
                                           tCTGCTGCAATGCGGCGATACCTCCAGTTTCACCCGTTTTATTGAGGAGATTGCCGATCGTTCACCACTACAAAAGGAGAGATATATGACTATTGCTGAACGGCTACGGCATGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGa                                                                                                          <  2:603122/150‑1 (MQ=37)
                                            ctgctgCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGaa                                                                                                         >  1:1305223/1‑150 (MQ=255)
                                            ctgctgCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAACGTAATCATGaa                                                                                                         >  2:161862/1‑150 (MQ=255)
                                             tgctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAAc                                                                                                        >  1:2033879/1‑150 (MQ=255)
                                               ctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAggac                                                                                                                                         <  2:450898/115‑3 (MQ=255)
                                               ctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAggac                                                                                                                                         >  1:450898/1‑113 (MQ=255)
                                                 gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGa                                                                                                                                              >  1:824116/1‑108 (MQ=255)
                                                 gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGa                                                                                                                                              <  2:824115/108‑1 (MQ=255)
                                                 gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTTGCAGGAATGTATGCATGAACAAGc                                                                                                    >  2:609342/1‑150 (MQ=255)
                                                 gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGc                                                                                                    >  2:684129/1‑150 (MQ=255)
                                                   aaTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCa                                                                                                  >  2:1883594/1‑150 (MQ=255)
                                                     tGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACAACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCAtt                                                                                                >  2:1697300/1‑150 (MQ=255)
                                                           gATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAAtt                                                                                          <  2:1743756/150‑1 (MQ=255)
                                                           gATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGCCTACGGCAGGAAGGCCAACAAATTGGCTGGCAGGAAGGTAAAAATCAACAATACATAaaaaaa                                                                                          >  1:1740793/1‑148 (MQ=21)
                                                                 tCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTg                                                                                    <  2:1173287/150‑1 (MQ=255)
                                                                 tCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTg                                                                                    <  1:1564981/150‑1 (MQ=255)
                                                                 tCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGATGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTg                                                                                    >  1:1861078/1‑150 (MQ=255)
                                                                    cGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTgcgc                                                                                 <  2:1380208/150‑1 (MQ=255)
                                                                           cccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTgg                                                                          <  2:442232/150‑1 (MQ=255)
                                                                                  tATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAggg                                                                   <  2:1790231/150‑1 (MQ=255)
                                                                                   aTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGGATGAACATTCGATTAAAATTGCTTTGATCATGCTTGAGCAggtc                                                                  >  2:1845530/1‑148 (MQ=25)
                                                                                    ttGAGGAGATTGCCGAACGTTCACCACTACAAACGGAGCGCTTAATGACTATTGCTGAACGGCTACGGCAGGAATGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCt                                                                 <  2:81285/150‑1 (MQ=255)
                                                                                    ttGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGATTGAAGATTAGATTTAAATAGCTATGATCATGCAGTAGCAGGTCt                                                                 >  2:73845/1‑150 (MQ=18)
                                                                                       aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTTCTTTGCGCATGCTGGAGCAGGGCTTTg                                                              <  1:1327736/150‑1 (MQ=255)
                                                                                       aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTg                                                              <  1:1351804/150‑1 (MQ=255)
                                                                                       aggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTg                                                              <  1:681232/150‑1 (MQ=255)
                                                                                        ggagATTGCCGAACGTTCACCACTACAAAAGGAGAGATTACTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGa                                                             <  2:1277393/150‑1 (MQ=255)
                                                                                           gaTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGAATGAACAAGCCATTAAAATTGCTTTGCACATGCTGGAGCAGGGCTTTGATCg                                                          >  1:1057469/1‑150 (MQ=255)
                                                                                            aTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGt                                                         <  1:1017605/150‑1 (MQ=255)
                                                                                                ccGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAATCCATTAAAATTGCTTTGCGCATGCTGAAGCAGGGCTTTGAACGTgaca                                                     >  2:1169521/1‑148 (MQ=255)
                                                                                                    aCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATtgt                                                 <  2:1740855/150‑1 (MQ=255)
                                                                                                     cGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAATCCATTAAAAATGCTTTTCTCATGCTGGAGCAGTGCTTTGAACGTGACATtgtg                                                >  2:1475537/1‑150 (MQ=25)
                                                                                                      gTTCACCACTACAAAATGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGc                                               <  2:1752754/150‑1 (MQ=255)
                                                                                                       ttCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGACATTGTGCt                                              >  1:1913670/1‑150 (MQ=255)
                                                                                                        tCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTg                                             <  1:1351313/150‑1 (MQ=255)
                                                                                                        tCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTAATAATGAACAAGCAATTAAAATAGCATTTATAATTCAGGAGCAGGTCATTGAACGTGACAATGAGCtc                                             >  2:774516/1‑149 (MQ=17)
                                                                                                             acTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGAc                                        <  2:462080/150‑1 (MQ=255)
                                                                                                              cTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAATTGCTTTGAGCATGCTGGAGCAGGGCTTTGAACGTGACAATGTGCTGGCgccc                                       >  2:46845/1‑147 (MQ=25)
                                                                                                              cTACAAAAGGACAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCTGCAATTTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAATTTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACa                                       <  2:1738639/150‑1 (MQ=37)
                                                                                                               tACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGAGCATCAAATTGGCAGTCAGGAAGCTATTCAAGATCAAGCCATTAAAATAGCTTTGCGCATGCTGGAACAGGGCTTTGAACGTGAGATTGTGCTGGCGACaa                                      >  1:1538894/1‑150 (MQ=25)
                                                                                                                      ggAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                  >  1:225369/1‑67 (MQ=255)
                                                                                                                       gatagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTTCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTc                              <  1:599911/147‑1 (MQ=255)
                                                                                                                         gagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACTTGAGATTGTGCTTGCGACAACCCACCTCAc                            >  1:1845692/1‑150 (MQ=255)
                                                                                                                         gagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCAc                            <  1:301220/150‑1 (MQ=255)
                                                                                                                          agcttAATGACTATTGCTGACCGGCTACGGCAGGAACGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACt                           <  1:997711/147‑1 (MQ=255)
                                                                                                                          agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAACTTGCTTTGCGCATGCTGTAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACt                           <  1:973818/150‑1 (MQ=255)
                                                                                                                            aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAAACCAACTCACTGa                         <  1:1259038/150‑1 (MQ=255)
                                                                                                                                 tGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTg                    <  2:102333/150‑1 (MQ=255)
                                                                                                                                  gACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGa                   <  1:1863727/150‑1 (MQ=255)
                                                                                                                                  gACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGa                   <  1:1864464/150‑1 (MQ=255)
                                                                                                                                  cacaaTTGCTGAACGGCTACGGCAGGACGGGCACCAATTTGGCTGGCAGGAAGGTATGCAAGAACAAGCCATAAAATTTGCTTTGCGCATGCGGGAGCAGGGCTTTGAACGTGAGATAGTGCTGGCGACAACCCAACACACTGATCCTGa                   <  2:406113/146‑1 (MQ=255)
                                                                                                                                     tATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGatat                <  1:1556675/150‑1 (MQ=255)
                                                                                                                                         tcTGATCGGCTACGGCAGGAAGGGCTTCTAATTGGCTGGCTGGAAGGTATGCTTGAACCAGCCATTTAAATTGCTTTGCGCATTCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCCCTGATGCTGATATTCCg            <  1:310581/149‑1 (MQ=255)
                                                                                                                                           tGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGaa          <  2:1387795/150‑1 (MQ=255)
                                                                                                                                            gAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGACCAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAAc         <  2:219953/150‑1 (MQ=255)
                                                                                                                                                 gCTACGGCATGAAGGGCATCAAATTGGCTGGGAGGAAGGTCTGCATGAACAAGCCATTATAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCa    <  2:1383960/150‑1 (MQ=255)
                                                                                                                                                  cTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAAAGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCAt   <  1:1385639/150‑1 (MQ=255)
                                                                                                                                                   tACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCAtt  >  2:97995/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGGCTTCACTAATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATT  >  NC_000913/2357505‑2357801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑