Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,417,344 A→T 5.0% E88D (GAA→GAT yfcC → putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,417,3440AT 5.0% 329.2 / 6.7 120E88D (GAA→GATyfcCputative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base A (59/51);  new base T (3/3);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 124 total aligned reads displayed.

GGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCCTGGC  >  NC_000913/2417195‑2417493
                                                                                                                                                     |                                                                                                                                                     
gggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGaa                                                                                                                                                       <  1:440039/150‑1 (MQ=255)
gggATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGaa                                                                                                                                                       >  1:361324/1‑150 (MQ=255)
  gATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACg                                                                                                                                                     >  2:14122/1‑150 (MQ=255)
   aTGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGc                                                                                                                                                    >  1:1818450/1‑150 (MQ=255)
      tttGACAGTCAGGAAGTGCAGTATCAGGTTGATGTTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCg                                                                                                                                                 <  2:1848016/150‑1 (MQ=255)
      tttGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCg                                                                                                                                                 <  2:1299207/150‑1 (MQ=255)
       ttGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCgg                                                                                                                                                <  1:141278/150‑1 (MQ=255)
        tGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCggg                                                                                                                                               >  1:960393/1‑150 (MQ=255)
          aCAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgt                                                                                                                                             <  1:1640739/150‑5 (MQ=255)
           cAGTCAGGAAGTGCTGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACTGGCGTTCATCGCCCgtcgtg                                                                                                                                            <  2:347864/150‑6 (MQ=255)
           cAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGTTCATCGCCCgtcgtg                                                                                                                                            <  2:382733/150‑6 (MQ=255)
            aGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATATCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCtg                                                                                                                                           <  1:55987/150‑1 (MQ=255)
               cAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGACCGCCCGGGCCtgatg                                                                                                                                        >  1:1505239/1‑150 (MQ=255)
                aGGAAGTGCAGTATCTGGTTGTTTGTTAAAAAAAAACAGTCTAAGTCGTAGATCTACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGTCGGGCGATGAACGCCCGGGCCtgatga                                                                                                                                       <  2:1837919/150‑1 (MQ=255)
                  gcaGTGCAGTATCAGGTTGATGCTCAAACAAAAACACGCAAAGTCGTTGATCCACACTCATTTCGCATTCTGACAAACGCAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAAc                                                                                                                                     <  1:912231/148‑1 (MQ=255)
                         aGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGt                                                                                                                              >  1:1160892/1‑150 (MQ=255)
                              cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGCCGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                         >  2:1304097/1‑150 (MQ=255)
                              cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                         >  1:1753012/1‑150 (MQ=255)
                              cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                         >  2:1819500/1‑150 (MQ=255)
                              cAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGaa                                                                                                                         >  1:214096/1‑150 (MQ=255)
                              cAGGTTGATGGTCAAACAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                        <  1:1461261/150‑1 (MQ=255)
                               cggTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                        <  2:1028179/149‑1 (MQ=255)
                               aGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAg                                                                                                                        <  1:501520/150‑1 (MQ=255)
                                gattGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAgg                                                                                                                       >  2:1205298/3‑150 (MQ=255)
                                  ttGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGAt                                                                                                                     <  1:1775321/150‑1 (MQ=255)
                                   tGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGAtt                                                                                                                    <  1:293170/150‑1 (MQ=255)
                                   tGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGAtt                                                                                                                    >  2:1860849/1‑150 (MQ=255)
                                      tGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAc                                                                                                                 >  1:520874/1‑150 (MQ=255)
                                       ggTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTCAGTATCACAGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGCCCTGATGAACATCCCGTTTGAAGGATTAAcc                                                                                                                >  2:436541/1‑150 (MQ=255)
                                        gTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCt                                                                                                               >  2:1566357/1‑150 (MQ=255)
                                        gTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCt                                                                                                               >  2:1723057/1‑150 (MQ=255)
                                          caaacaaaAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACAGCGTACAGCAGTTCACGACGGGCGATGAACGCCCAGCCCTGCTGACCTTCCCGTTTGAAGGATTAACCTCa                                                                                                             >  1:333062/1‑150 (MQ=255)
                                            aacaaaAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAgg                                                                                                           >  2:600902/1‑150 (MQ=255)
                                              caaaAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCAGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGAt                                                                                                         >  2:577349/1‑150 (MQ=255)
                                               aaaaaaACGCAAAGACGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATc                                                                                                        <  2:664816/150‑1 (MQ=255)
                                               aaaaaCACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATc                                                                                                        <  2:1403084/150‑1 (MQ=255)
                                                aaaaCACGCATAGTCTTAGATCAACACTCATTTCGCATTCTGACTAACGAATCAGGCGAACCTGAGTTTCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGTAGGATTAACCTCAGGATCg                                                                                                       <  2:746957/150‑1 (MQ=255)
                                                  aaCACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACCGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGaa                                                                                                     >  1:82516/1‑150 (MQ=255)
                                                    cacGCAAAGTCGTAGATCCACACTCATTTCGGATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAAt                                                                                                   <  2:1350272/150‑1 (MQ=255)
                                                     acGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATa                                                                                                  <  1:1237307/150‑1 (MQ=255)
                                                        cAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTATCGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACgg                                                                                               <  1:1473036/150‑1 (MQ=255)
                                                         aaaGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACggg                                                                                              <  1:1053731/150‑1 (MQ=255)
                                                              cGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGc                                                                                         >  1:1682342/1‑150 (MQ=255)
                                                              cGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGAACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCACAGGATCGAAATACGGGACAGc                                                                                         >  2:386813/1‑150 (MQ=255)
                                                                 aGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGACCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGAGCGAAATACGGGACAGCCGt                                                                                      >  2:1227730/1‑150 (MQ=255)
                                                                      cacaCTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGca                                                                                 >  2:880955/1‑150 (MQ=255)
                                                                      cacaCTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGca                                                                                 <  2:772236/150‑1 (MQ=255)
                                                                       acacTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcat                                                                                >  2:1970659/1‑150 (MQ=255)
                                                                        cacTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCCCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTAGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatc                                                                               >  1:1522621/1‑150 (MQ=255)
                                                                        cacTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTAAAGCTGTTCACGACGGGCTATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcagc                                                                               >  2:1381691/1‑148 (MQ=255)
                                                                           tCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcatc                                                                            <  2:1753011/150‑1 (MQ=255)
                                                                           tCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcatc                                                                            <  2:214096/150‑1 (MQ=255)
                                                                            cATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcatca                                                                           >  2:203256/1‑150 (MQ=255)
                                                                             aTTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcatcat                                                                          >  1:1158684/1‑150 (MQ=255)
                                                                             aTTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGcatcatcat                                                                          >  2:1814790/1‑150 (MQ=255)
                                                                                  gctttCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTa                                                                     <  2:1068332/147‑1 (MQ=255)
                                                                                    aTTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATg                                                                   >  1:903982/1‑150 (MQ=255)
                                                                                       cTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTg                                                                >  1:340695/1‑150 (MQ=255)
                                                                                        tGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTgg                                                               >  1:246216/1‑150 (MQ=255)
                                                                                          aCTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTg                                                             <  2:875236/150‑1 (MQ=255)
                                                                                           cTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                            >  1:1917639/1‑150 (MQ=255)
                                                                                           atAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGa                                                            <  1:1506624/149‑1 (MQ=255)
                                                                                            tAACGAAGCAGGCGACCCTGAGTATCAACGCGTACAGCTGTTCACGACGGGCGATGACCGCCCGGGACTGATGAACTTGCCGTTTGAAGGATTTACCTCCGGATCGAAATACGGGACAGCCGTAGGCCTCATCAGGTTTATGCTGGTGAt                                                           >  2:1284040/1‑150 (MQ=255)
                                                                                              aCGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTg                                                         >  2:1536028/1‑150 (MQ=255)
                                                                                               cGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTgg                                                        <  1:331986/150‑1 (MQ=255)
                                                                                                         caaCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGttt                                              <  1:1964663/149‑1 (MQ=255)
                                                                                                          aaCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTg                                             <  1:577349/150‑1 (MQ=255)
                                                                                                          aaCCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTg                                             <  1:14122/150‑1 (MQ=255)
                                                                                                            ccTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGGGATTGGCGGCGCGTTTGGc                                           <  2:1124435/150‑1 (MQ=255)
                                                                                                             cTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCa                                          <  2:1556164/150‑1 (MQ=255)
                                                                                                              tGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAt                                         >  2:1541884/1‑150 (MQ=255)
                                                                                                               gAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCAtt                                        >  2:754722/1‑150 (MQ=255)
                                                                                                                    tCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGAt                                   >  1:1320479/1‑150 (MQ=255)
                                                                                                                    tCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGAt                                   >  2:1648018/1‑150 (MQ=255)
                                                                                                                    tCACCGCGTACAGCTGTTCACGACGGCCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAATGATTAACCTCCGGATCCAAATACGGGACAGCCGTTGGCATCATCATGATTATGCTGGTGATTGGCGGCGCGTTTGGCagattgat                                   >  2:92895/1‑143 (MQ=255)
                                                                                                                     cACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATg                                  <  2:1224902/150‑1 (MQ=255)
                                                                                                                      aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg                                     <  2:44250/146‑1 (MQ=255)
                                                                                                                      aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGc                                 >  2:407028/1‑150 (MQ=255)
                                                                                                                      aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGc                                 >  1:265155/1‑150 (MQ=255)
                                                                                                                      aCCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGAAGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAAAACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATtgtg                                     >  1:44250/1‑146 (MQ=255)
                                                                                                                       ccGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGTCATTGTGATGCg                                >  2:48225/1‑150 (MQ=255)
                                                                                                                              cAGCTGTTCACGACGGGCGATGACCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGACATACGGGCCAGCCGGTGGCATCATCATGATTATGCTGGTTATTGGCGGCGCGATTGGCATTGTGATGCGTACAGGa                         >  1:406307/1‑150 (MQ=255)
                                                                                                                              cAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGa                         >  1:1979440/1‑150 (MQ=255)
                                                                                                                               aGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGaa                        <  1:1593433/150‑1 (MQ=255)
                                                                                                                                gCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAAc                       >  2:878825/1‑150 (MQ=255)
                                                                                                                                   gTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAt                    >  1:1870835/1‑150 (MQ=255)
                                                                                                                                    ttCACGCCGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGGGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCAtt                   <  2:1039069/150‑1 (MQ=255)
                                                                                                                                     tCACGACGGGCGATGAACGCCCGGGACTGATGAACTTCCCTTTTGAATGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTg                  <  2:333062/150‑1 (MQ=255)
                                                                                                                                      cACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGa                 <  1:1757354/150‑1 (MQ=255)
                                                                                                                                      cACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGa                 >  2:654077/1‑150 (MQ=255)
                                                                                                                                       ggcccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGAt                >  1:114474/5‑150 (MQ=255)
                                                                                                                                        cgacgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATa               <  2:1407147/150‑1 (MQ=255)
                                                                                                                                         cccgGGCGTTCATCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATaa              >  2:442320/3‑150 (MQ=255)
                                                                                                                                          acgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAAc             <  1:1359505/150‑1 (MQ=255)
                                                                                                                                           cgGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACg            >  2:1915637/1‑150 (MQ=255)
                                                                                                                                             ggCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACATCCGTTGGCATCATCATGTTTATGCTGGTTATTGGCGGCGCGGTTTGCATTGTTATGCGTACAGGAACCATTGATAACGGt          >  1:269895/1‑150 (MQ=255)
                                                                                                                                                 aTGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATcc      <  1:754723/150‑1 (MQ=255)
                                                                                                                                                  tGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCCGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCCt     >  2:1343659/1‑150 (MQ=255)
                                                                                                                                                    aaCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAAGCTGAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGTACCATTGTTAACGGTATCCTgg   <  1:436541/150‑1 (MQ=255)
                                                                                                                                                     aCGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGCCAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGGATTGTGATGCGTCCAGGAACCATTGATAACGGTATCCTGgc  >  2:286693/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGATGTTTGACAGTCAGGAAGTGCAGTATCAGGTTGATGGTCAAACAAAAACACGCAAAGTCGTAGATCCACACTCATTTCGCATTCTGACTAACGAAGCAGGCGAACCTGAGTATCACCGCGTACAGCTGTTCACGACGGGCGATGAACGCCCGGGCCTGATGAACTTCCCGTTTGAAGGATTAACCTCAGGATCGAAATACGGGACAGCCGTTGGCATCATCATGTTTATGCTGGTGATTGGCGGCGCGTTTGGCATTGTGATGCGTACAGGAACCATTGATAACGGTATCCTGGC  >  NC_000913/2417195‑2417493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: