Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,533,415 T→A 5.0% intergenic (+35/‑349) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,533,4150TA 5.0% 352.4 / 4.6 114intergenic (+35/‑349)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base T (51/56);  new base A (2/4);  total (54/60)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 117 total aligned reads displayed.

AGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGA  >  NC_000913/2533266‑2533562
                                                                                                                                                     |                                                                                                                                                   
 gaTGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAGCACCtttt                                                                                                                                                   >  1:1978882/1‑150 (MQ=255)
  aTGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGGGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCtttt                                                                                                                                                   <  2:1886502/150‑1 (MQ=255)
    gAAAGCTTTACCAACAAGAATATTGTTGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCTATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAATAGCACCTTTTTa                                                                                                                                                 <  1:290282/150‑1 (MQ=255)
    gAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTa                                                                                                                                                 >  2:1637408/1‑150 (MQ=255)
     aaaGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAg                                                                                                                                                >  2:1374636/1‑150 (MQ=255)
      aaGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAgg                                                                                                                                               <  1:1838533/150‑1 (MQ=255)
          tttACCAAGTAGATTATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCt                                                                                                                                           <  2:1229990/150‑1 (MQ=255)
            tACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCttt                                                                                                                                         >  2:625912/1‑150 (MQ=255)
              ccAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCATAAAAAAGCACCTTTTTAGGTGCttttt                                                                                                                                       <  2:1045419/150‑1 (MQ=255)
                aacaaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtg                                                                                                                                     <  2:266739/150‑1 (MQ=255)
                 acaaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTAAAAAGGTGCttttttac                                                                                                                                    <  2:1583474/150‑3 (MQ=255)
                  caaGATTATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACGTTTTTAGGTGCTTTTTtgtg                                                                                                                                   <  2:261962/150‑1 (MQ=255)
                  caaGAATATTGTGGTTATTCTACCATCCTCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtgtg                                                                                                                                   <  1:1463390/150‑1 (MQ=255)
                  aaataaTATTGTTGGTATTCTACCATCATGGGGTGAGCGTTATTTAAGCACCGCTTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTAAAAAGGTGCttttttacg                                                                                                                                   <  2:1859604/146‑4 (MQ=255)
                   aaGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTAATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTgg                                                                                                                                  <  1:256269/150‑1 (MQ=255)
                     gAATATTGTGGTTATTCTACCATCATCGGGGGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGcc                                                                                                                                <  1:1906821/150‑1 (MQ=255)
                           tgtgGTTATTATACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTATAGGTGCTTTTTTGTGGCCTGCTTc                                                                                                                          >  1:1272388/1‑150 (MQ=255)
                            gtgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCa                                                                                                                         <  2:1171207/150‑1 (MQ=255)
                              ggTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCaaa                                                                                                                       <  2:541458/150‑1 (MQ=255)
                                ttATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACt                                                                                                                     <  1:244111/150‑1 (MQ=255)
                                   ttCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTc                                                                                                                  >  1:501921/1‑150 (MQ=255)
                                    tCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCg                                                                                                                 <  2:507928/150‑1 (MQ=255)
                                     cTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCAAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                                                                                >  2:1541221/1‑150 (MQ=255)
                                      tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                                                                               >  2:1467449/1‑150 (MQ=255)
                                      tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                                                                               >  2:1822009/1‑150 (MQ=255)
                                            catcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCGGATCTCTTCAGTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCTTAAAAAAGCACCTTTTTAGGAGCTTTTTTGTGTCATGCTTCAAACGTTCGCCcctcct                                                                                                         >  2:1280863/1‑150 (MQ=255)
                                             atcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTg                                                                                                        >  1:1622301/1‑150 (MQ=255)
                                                 ggTGAGCGGTAGTTAAGCACCGCATTGTTTGCCCATCTCATCCCTGAGAAAGCATTGCAACAGTAAAGCCCGCTGGGTAAAAATGCGTATAACAGCCCCTGATTAGGTGCTTTTATGTGGCCTGCTTCACACTTTCTCCCCTCCTGGCAt                                                                                                    >  1:91866/1‑150 (MQ=255)
                                                  gTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                                                                                   <  2:444278/150‑1 (MQ=255)
                                                  gTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                                                                                   <  2:1902784/150‑1 (MQ=255)
                                                  gTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                  <  2:1978881/150‑1 (MQ=255)
                                                   tGAGCGTTATTTAATCACCGCATTGTTTGCCGATCTCTTCAGTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTTAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTg                                                                                                  <  1:744109/150‑1 (MQ=255)
                                                        gTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCa                                                                                             <  1:1995823/150‑1 (MQ=255)
                                                         ttATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                                                                            >  1:1626921/1‑150 (MQ=255)
                                                         ttATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                                                                            <  2:1452420/150‑1 (MQ=255)
                                                           ttttAAGCACCGCATTTTTTGCCGATCTCTTCACTGAGATAGAATTGCTACAGTAATGCCAGCTTGTTAAAAATTCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                          <  1:646114/149‑1 (MQ=255)
                                                           aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAAAGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                                                                                          >  1:979538/1‑150 (MQ=255)
                                                            tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                                                                                         <  2:1423013/150‑1 (MQ=255)
                                                            tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                                                                                         >  1:1625865/1‑150 (MQ=255)
                                                            tttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCATGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                                                                                         >  2:648982/1‑150 (MQ=255)
                                                             ttAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTg                                                                                        <  1:740136/150‑1 (MQ=255)
                                                               aaGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCGAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGTCATTGATTCAGCCTGTc                                                                                      <  1:151155/150‑1 (MQ=255)
                                                               aaGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCCCCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTc                                                                                      >  2:2019374/1‑150 (MQ=255)
                                                                aGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCg                                                                                     <  1:1518594/150‑1 (MQ=255)
                                                                aGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTATTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCg                                                                                     >  2:1094461/1‑150 (MQ=255)
                                                                    ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCATACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                                                                                 >  1:1347314/1‑150 (MQ=255)
                                                                    ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                                                                                 >  1:1435447/1‑150 (MQ=255)
                                                                     cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                                                                                >  2:284783/1‑150 (MQ=255)
                                                                      gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTg                                                                               >  1:41372/1‑150 (MQ=255)
                                                                        aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGt                                                                             <  2:243563/150‑1 (MQ=255)
                                                                          tGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAt                                                                           >  1:1162061/1‑150 (MQ=255)
                                                                           gTTTGCCGTTCTCTTCACTGAGAAAGAATTGCAACCGTAATGCCAGCTTGTTAATAATGCGTATAAAAGCACCTTTTTAGGTGCTTTTTTGTTGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGTTCTGGTAtt                                                                          <  1:1131755/150‑1 (MQ=255)
                                                                           gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGTCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                          <  1:1722430/150‑1 (MQ=255)
                                                                           gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                          <  2:1486326/150‑1 (MQ=255)
                                                                           gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                          <  1:1265747/150‑1 (MQ=255)
                                                                           gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCATGCTTCAAACTTTCGCCCCTCATGGCATTGATTCAGCCTGTCGGAACTGGTAtt                                                                          >  2:1563110/1‑150 (MQ=255)
                                                                               gCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGACTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTatc                                                                      >  2:756531/1‑148 (MQ=255)
                                                                                ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAcc                                                                     <  1:1635489/150‑1 (MQ=255)
                                                                                 cGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCa                                                                    >  1:1317082/1‑150 (MQ=255)
                                                                                  gATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg                                                                   >  1:847525/1‑150 (MQ=255)
                                                                                  gATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg                                                                   <  1:1438295/150‑1 (MQ=255)
                                                                                      tctTCACTGAGAAAGAATTGCAATAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTa                                                               >  2:1742180/1‑150 (MQ=255)
                                                                                      tctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTa                                                               <  1:524561/150‑1 (MQ=255)
                                                                                      tctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTa                                                               <  2:66790/150‑1 (MQ=255)
                                                                                         tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAatt                                                            <  2:979537/150‑1 (MQ=255)
                                                                                            cTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAattatt                                                         <  2:899177/150‑1 (MQ=255)
                                                                                              gagaAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAtttt                                                       <  2:993746/150‑1 (MQ=255)
                                                                                              gagaAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTAtttt                                                       >  1:1855286/1‑150 (MQ=255)
                                                                                                gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                     >  1:1109131/1‑150 (MQ=255)
                                                                                                gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGa                                                     >  1:431667/1‑150 (MQ=255)
                                                                                                    gaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCACTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATgcg                                                 >  2:1377376/1‑150 (MQ=255)
                                                                                                     atttGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCCCCTTTGTACGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAGCAGACTAATTATTTTGATgcgc                                                >  2:43062/3‑150 (MQ=255)
                                                                                                         gCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                            <  2:966168/150‑1 (MQ=255)
                                                                                                         gCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                            >  1:1534796/1‑150 (MQ=255)
                                                                                                         gCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGaaa                                            >  1:1873522/1‑150 (MQ=255)
                                                                                                          cAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAAt                                           >  1:1289348/1‑150 (MQ=255)
                                                                                                                tAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCATGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCg                                     >  1:1655016/1‑150 (MQ=255)
                                                                                                                  aTGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCGTTTTAGGTGCTTTTTTGTGGCCTGCTTCATACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGtt                                   <  2:109917/150‑1 (MQ=255)
                                                                                                                   tGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTa                                  >  1:900962/1‑150 (MQ=255)
                                                                                                                   tGCCAGCTTGTTAAAAATGCGTAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTATTTATTTTGATGCGCGAAATTAATCGTTAc                                 >  2:1237079/1‑150 (MQ=255)
                                                                                                                        gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGa                             <  1:1066366/150‑1 (MQ=255)
                                                                                                                        gCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGa                             <  1:1073481/150‑1 (MQ=255)
                                                                                                                              tAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                       <  2:1855285/150‑1 (MQ=255)
                                                                                                                              tAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGcc                       >  2:563557/1‑150 (MQ=255)
                                                                                                                               aaaaaTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCa                      >  1:1336617/1‑150 (MQ=255)
                                                                                                                                   aTGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGc                  <  2:1130775/150‑1 (MQ=255)
                                                                                                                                    tGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCt                 <  2:41372/150‑1 (MQ=255)
                                                                                                                                     gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTg                >  1:1705630/1‑150 (MQ=255)
                                                                                                                                     gCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTg                <  1:1965479/150‑1 (MQ=255)
                                                                                                                                        tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt             >  2:420492/1‑150 (MQ=255)
                                                                                                                                        tAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATATTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAt             >  2:995292/1‑150 (MQ=255)
                                                                                                                                         aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATc            >  2:1642341/1‑150 (MQ=255)
                                                                                                                                         aaaaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATc            >  2:684134/1‑150 (MQ=255)
                                                                                                                                         aaaaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCACTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAAta            >  1:1668703/1‑149 (MQ=255)
                                                                                                                                           aaaaGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCTAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGa          >  2:1620516/1‑150 (MQ=255)
                                                                                                                                               gCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTTATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGAtttt      >  2:1077839/1‑150 (MQ=255)
                                                                                                                                                 aCCTTTTTAGGTGCTTTTTTGTGGCCTGCTGCAAACTTTCGCCCCTCCGGGCATTGATTCAGACTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTAt    <  2:1321709/150‑1 (MQ=255)
                                                                                                                                                  ccTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg   >  1:423050/1‑150 (MQ=255)
                                                                                                                                                  ccTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg   >  1:1351814/1‑150 (MQ=255)
                                                                                                                                                  ccTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGAATAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATg   >  1:187918/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAATTATTTTGATGCGCGAAATTAATCGTTACAGGAAAAGCCAAAGCTGAATCGATTTTATGA  >  NC_000913/2533266‑2533562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: