Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,201 T→C 7.6% intergenic (‑19/+27) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2010TC 7.6% 291.4 / 12.3 105intergenic (‑19/+27)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base T (51/45);  new base C (4/4);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 108 total aligned reads displayed.

CATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCC  >  NC_000913/2555053‑2555347
                                                                                                                                                    |                                                                                                                                                  
cATGTTCATAGACATCCGCTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGGTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGtgttg                                                                                                                                                   <  1:1241588/150‑1 (MQ=255)
  tGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTa                                                                                                                                                 <  2:162638/150‑1 (MQ=255)
  tGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTa                                                                                                                                                 >  1:162638/1‑150 (MQ=255)
   gTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGTGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                <  1:1859573/150‑1 (MQ=255)
    ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                               <  1:482249/150‑1 (MQ=255)
    ttCATAGACATCCGTTGGCTGTCGTTGATGAACATTATCCAGTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                               <  1:1020570/150‑1 (MQ=255)
     tCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                              >  1:920024/1‑150 (MQ=255)
      cATAGACATCCGTTGTCTGTCGTTGAAGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCTGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACCTGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                             >  1:204218/1‑150 (MQ=255)
          gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACAccc                                                                                                                                         <  1:1232002/150‑1 (MQ=255)
           aCATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                       <  2:639427/150‑1 (MQ=255)
            cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                       <  1:1740114/150‑1 (MQ=255)
            cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                       <  2:1700758/150‑1 (MQ=255)
            cATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCgg                                                                                                                                       >  1:254424/1‑150 (MQ=255)
             aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                      <  1:1530521/150‑1 (MQ=255)
              tCCGTTGGCTGTCGTTGATGAACTTTATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTTGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                     <  2:509587/150‑1 (MQ=255)
               ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                    >  1:1624634/1‑150 (MQ=255)
                ctttGTCTGTCGGTGATGAACATTATCCACTTCGCCCTTCGGCGTGAGCTTCAGGCTTTCGGCTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                   <  1:471338/148‑1 (MQ=255)
                cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                   >  1:565019/1‑150 (MQ=255)
                 gTTGTCTGTCGTTGTTGATCATTATACACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGTTTCATGATAAAGATGGTGCAAATTTGCTGTACGTGTCTTTTTCATGATGTTAATGCCGGGGGTCCTACAACACCCGGCatta                                                                                                                                  <  2:1156542/150‑4 (MQ=255)
                   tgtctgtcGTTGAGGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                <  1:4830/150‑1 (MQ=255)
                    gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                               >  2:112915/1‑150 (MQ=255)
                    gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                               <  2:49956/150‑1 (MQ=255)
                    gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCGGTCCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                               <  1:390487/150‑1 (MQ=255)
                       tgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACa                                                                                                                            >  1:693337/1‑150 (MQ=255)
                          cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                         >  1:1384849/1‑150 (MQ=255)
                           gTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGtt                                                                                                                        <  1:1336783/150‑1 (MQ=255)
                              gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                     >  2:1744424/1‑150 (MQ=255)
                              gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                     >  2:817471/1‑150 (MQ=255)
                                tgaACATTATCCACTTCGACCTTCGGCGTGCGCTTCGGGTTTTGGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGCCACCCGGCACCTCCGACAGGTTAATgg                                                                                                                   >  2:590555/1‑150 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGTCACCTCCGCCACGTTAATGGGGc                                                                                                                >  2:124944/1‑150 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                <  2:491309/150‑1 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGAACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                >  2:37248/1‑150 (MQ=255)
                                     aTTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCtt                                                                                                              >  1:213620/1‑150 (MQ=255)
                                      ttATCCTCTTCGTCCTTCGCCGTTAGCTTGAGGTTTTCGGGTAAGGGTTCATGTTAAAGTTTGTGAAAATGGGCTGTACGGGTATTTTTAATGAGGTTAATGCCGGGTGGTGTCGGACACCCGGCACCTCCTACAGGTTAATGGGGCGTg                                                                                                             <  2:1912072/150‑1 (MQ=255)
                                      ttATCCACTTCGTCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                             <  1:1093527/150‑1 (MQ=255)
                                      ttATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                             <  2:609999/150‑1 (MQ=255)
                                      ttATCCACTTCGACCTTCGGCGGGAGCTGCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                             <  2:1386037/150‑1 (MQ=255)
                                       tATCCACTTCGTACTTCGTCGTTAGCTTCTGGTTTTCGTGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                            <  2:1056897/150‑1 (MQ=255)
                                       tATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                            <  2:1113373/150‑1 (MQ=255)
                                         tCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGATTgaga                                                                                                          >  1:402621/1‑150 (MQ=255)
                                         tCCACTTCGACCTTCGGCGTGAGCTTAAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATTCCGGGTGTTGTAGGACACACGGCACCTCCGACAGGTTAATTGGGCTTgaga                                                                                                          >  1:1720931/1‑150 (MQ=255)
                                          ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                         >  2:1996901/1‑150 (MQ=255)
                                            aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                       >  2:939978/1‑150 (MQ=255)
                                             cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                      >  1:486222/1‑150 (MQ=255)
                                                cGACCTTCGGCGTTAGGTTCAGGTTTTCGGGTAAGGGGTCATGATAACGATGGTGCAATTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAAc                                                                                                   <  1:992822/150‑1 (MQ=255)
                                                        ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                           >  1:1641011/1‑150 (MQ=255)
                                                        ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                           >  2:260070/1‑150 (MQ=255)
                                                            tGAGCTTCTGTTTTTCGGTTAAGGGTTCTTCAAAACGATGGTGCAAATTGGCTGTACGGGTCTTTTACATCTTGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGtt                                                                                       <  2:1505088/150‑1 (MQ=255)
                                                               gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTAc                                                                                    <  2:150995/150‑1 (MQ=255)
                                                               gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTAc                                                                                    >  2:125571/1‑150 (MQ=255)
                                                                gttatggTTTTCGGTTAATGGTTAATTATAAATATGTTACAAATTGGCTGTTCGGGGTTTTTTCTTGTTTTTATTCCGGGTTGTTGTCTGCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACTATAACGACTACTGCGTTTACg                                                                                   <  2:696691/145‑1 (MQ=255)
                                                                cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                   >  1:17528/1‑150 (MQ=255)
                                                                cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATCGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGTTGTTGTAGGACACACGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAACTACAAAAGCGTTTACg                                                                                   >  1:640737/1‑150 (MQ=255)
                                                                  tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                 <  1:1606708/150‑1 (MQ=255)
                                                                  tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                 <  1:893787/150‑1 (MQ=255)
                                                                     ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACg                                                                              >  1:1165899/1‑150 (MQ=255)
                                                                        tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                           >  1:1372590/1‑150 (MQ=255)
                                                                             ggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGTTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTACGTAACGTCCCGGCa                                                                      >  1:1027871/1‑150 (MQ=255)
                                                                              gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAg                                                                     <  1:1096511/150‑1 (MQ=255)
                                                                               tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTAGGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                    <  2:815053/150‑1 (MQ=255)
                                                                                    gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                              >  1:1636279/1‑150 (MQ=255)
                                                                                         tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTATACGTAACGTCACGGCAGAAAAGAGCTGt                                                          >  1:493686/1‑150 (MQ=255)
                                                                                           aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                        >  1:540145/1‑150 (MQ=255)
                                                                                           aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                        <  1:605217/150‑1 (MQ=255)
                                                                                            tAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCg                                                       <  1:694824/150‑1 (MQ=255)
                                                                                              aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                     >  2:20331/1‑150 (MQ=255)
                                                                                               aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                    >  1:1085407/1‑150 (MQ=255)
                                                                                               aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                    <  2:273738/150‑1 (MQ=255)
                                                                                                    gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAtt                                               >  1:1748926/1‑150 (MQ=255)
                                                                                                        aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                           >  2:473357/1‑150 (MQ=255)
                                                                                                        aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                           <  2:936738/150‑1 (MQ=255)
                                                                                                          aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                         <  2:1641010/150‑1 (MQ=255)
                                                                                                          aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                         >  2:428889/1‑150 (MQ=255)
                                                                                                           ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                        <  1:1143189/150‑1 (MQ=255)
                                                                                                              gCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCtttt                                     <  1:817472/150‑1 (MQ=255)
                                                                                                               cTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttttt                                    <  1:260070/150‑1 (MQ=255)
                                                                                                                    cGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                               >  2:101395/1‑150 (MQ=255)
                                                                                                                      ggTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACTTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                             <  2:1768463/150‑1 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTTTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                           <  2:1784170/150‑1 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                           >  2:698505/1‑150 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGCGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGATCGAGCGCATTTCTGCCTTTTTCCCGCGgcc                           >  2:1806225/1‑150 (MQ=255)
                                                                                                                        gcTTTTTCATGAAGTTATTGCCGGGTGTTGGAGGACAGCCGGCTACTCCGTCAGGTTAATGGGGCTTGAGTCGATAACGACTACTGCGTTTACGTTACGTCCCGGCAGTATAGAGCTGTTCGAGCGGATTTCTGGCTTTTTCGAGCGgcc                           <  2:357100/149‑1 (MQ=255)
                                                                                                                            tttCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGGTAATGGGGCCTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                       <  1:336026/150‑1 (MQ=255)
                                                                                                                             ttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                      >  1:813593/1‑150 (MQ=255)
                                                                                                                                atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                   >  1:861627/1‑150 (MQ=255)
                                                                                                                                   atgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTgg                >  2:365476/1‑150 (MQ=255)
                                                                                                                                    tgTAAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg               <  1:720755/150‑1 (MQ=255)
                                                                                                                                     gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc              <  2:803203/150‑1 (MQ=255)
                                                                                                                                     gTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc              <  2:254424/150‑1 (MQ=255)
                                                                                                                                         gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGCTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc          >  2:1978455/2‑150 (MQ=255)
                                                                                                                                         gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACAACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCGGTTCGAGCGCATTTCTGGATTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc          >  2:1356189/2‑150 (MQ=255)
                                                                                                                                         aTGCCGGGGGTTGTAGGACACCGGGCACCTCCGACCGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTTCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc          <  2:978909/150‑1 (MQ=255)
                                                                                                                                          tGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGGCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCa         >  2:2148/1‑150 (MQ=255)
                                                                                                                                           gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGCAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCCGCCAAAGTGGGCAGCCag        >  1:387042/1‑149 (MQ=255)
                                                                                                                                           gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc        >  2:1817097/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt       >  2:660817/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt       <  2:1445912/150‑1 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTACAGCGGCCAGCCAAAGTGGGCAGCCACt       >  1:1173933/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt       >  2:447472/1‑150 (MQ=255)
                                                                                                                                             cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGCCACtt      >  1:807272/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCC  >  NC_000913/2555053‑2555347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: