Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,202 G→C 9.3% intergenic (‑20/+26) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2020GC 9.3% 315.7 / 18.2 105intergenic (‑20/+26)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base G (50/44);  new base C (5/5);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 110 total aligned reads displayed.

CATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCC  >  NC_000913/2555053‑2555351
                                                                                                                                                     |                                                                                                                                                     
cATGTTCATAGACATCCGCTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGGTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGtgttg                                                                                                                                                       <  1:1241588/150‑1 (MQ=255)
  tGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTa                                                                                                                                                     >  1:162638/1‑150 (MQ=255)
  tGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTa                                                                                                                                                     <  2:162638/150‑1 (MQ=255)
   gTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGTGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                    <  1:1859573/150‑1 (MQ=255)
   gTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTACGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATCGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGtagtag                                                                                                                                                    >  2:517090/1‑150 (MQ=255)
    ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                   <  1:482249/150‑1 (MQ=255)
    ttCATAGACATCCGTTGGCTGTCGTTGATGAACATTATCCAGTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                   <  1:1020570/150‑1 (MQ=255)
     tCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                                  >  1:920024/1‑150 (MQ=255)
      cATAGACATCCGTTGTCTGTCGTTGAAGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCTGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACCTGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                 >  1:204218/1‑150 (MQ=255)
          gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACAccc                                                                                                                                             <  1:1232002/150‑1 (MQ=255)
           aCATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                           <  2:639427/150‑1 (MQ=255)
            cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                           <  2:1700758/150‑1 (MQ=255)
            cATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCgg                                                                                                                                           >  1:254424/1‑150 (MQ=255)
             aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                          <  1:1530521/150‑1 (MQ=255)
              tCCGTTGGCTGTCGTTGATGAACTTTATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTTGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                         <  2:509587/150‑1 (MQ=255)
               ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                        >  1:1624634/1‑150 (MQ=255)
                ctttGTCTGTCGGTGATGAACATTATCCACTTCGCCCTTCGGCGTGAGCTTCAGGCTTTCGGCTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                       <  1:471338/148‑1 (MQ=255)
                cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                       >  1:565019/1‑150 (MQ=255)
                 gTTGTCTGTCGTTGTTGATCATTATACACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGTTTCATGATAAAGATGGTGCAAATTTGCTGTACGTGTCTTTTTCATGATGTTAATGCCGGGGGTCCTACAACACCCGGCatta                                                                                                                                      <  2:1156542/150‑4 (MQ=255)
                   tgtctgtcGTTGAGGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                    <  1:4830/150‑1 (MQ=255)
                    gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                   <  2:49956/150‑1 (MQ=255)
                    gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                   >  2:112915/1‑150 (MQ=255)
                       tgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACa                                                                                                                                >  1:693337/1‑150 (MQ=255)
                          cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                             >  1:1384849/1‑150 (MQ=255)
                              gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                         >  2:1744424/1‑150 (MQ=255)
                              gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                         >  1:1524221/1‑150 (MQ=255)
                              gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                         >  2:817471/1‑150 (MQ=255)
                                tgaACATTATCCACTTCGACCTTCGGCGTGCGCTTCGGGTTTTGGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGCCACCCGGCACCTCCGACAGGTTAATgg                                                                                                                       >  2:590555/1‑150 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGTCACCTCCGCCACGTTAATGGGGc                                                                                                                    >  2:124944/1‑150 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                    <  2:491309/150‑1 (MQ=255)
                                   aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGAACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                    >  2:37248/1‑150 (MQ=255)
                                     aTTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCtt                                                                                                                  >  1:213620/1‑150 (MQ=255)
                                      ttATCCTCTTCGTCCTTCGCCGTTAGCTTGAGGTTTTCGGGTAAGGGTTCATGTTAAAGTTTGTGAAAATGGGCTGTACGGGTATTTTTAATGAGGTTAATGCCGGGTGGTGTCGGACACCCGGCACCTCCTACAGGTTAATGGGGCGTg                                                                                                                 <  2:1912072/150‑1 (MQ=255)
                                      ttATCCACTTCGTCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                 <  1:1093527/150‑1 (MQ=255)
                                      ttATCCACTTCGACCTTCGGCGGGAGCTGCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                 <  2:1386037/150‑1 (MQ=255)
                                       tATCCACTTCGTACTTCGTCGTTAGCTTCTGGTTTTCGTGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                <  2:1056897/150‑1 (MQ=255)
                                       tATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                <  2:1113373/150‑1 (MQ=255)
                                         tCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGATTgaga                                                                                                              >  1:402621/1‑150 (MQ=255)
                                         tCCACTTCGACCTTCGGCGTGAGCTTAAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATTCCGGGTGTTGTAGGACACACGGCACCTCCGACAGGTTAATTGGGCTTgaga                                                                                                              >  1:1720931/1‑150 (MQ=255)
                                          ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                             >  2:1996901/1‑150 (MQ=255)
                                            aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                           >  2:939978/1‑150 (MQ=255)
                                             cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                          >  1:486222/1‑150 (MQ=255)
                                                        ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                               >  1:1641011/1‑150 (MQ=255)
                                                        ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                               >  2:260070/1‑150 (MQ=255)
                                                               gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTAc                                                                                        <  2:150995/150‑1 (MQ=255)
                                                               gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTAc                                                                                        >  2:125571/1‑150 (MQ=255)
                                                                gttatggTTTTCGGTTAATGGTTAATTATAAATATGTTACAAATTGGCTGTTCGGGGTTTTTTCTTGTTTTTATTCCGGGTTGTTGTCTGCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACTATAACGACTACTGCGTTTACg                                                                                       <  2:696691/145‑1 (MQ=255)
                                                                cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                       >  1:17528/1‑150 (MQ=255)
                                                                  tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                     <  1:893787/150‑1 (MQ=255)
                                                                  tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                     <  1:1606708/150‑1 (MQ=255)
                                                                  tCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                     <  2:15879/150‑1 (MQ=255)
                                                                     ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACg                                                                                  >  1:1165899/1‑150 (MQ=255)
                                                                        tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                               >  1:1372590/1‑150 (MQ=255)
                                                                             ggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGTTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTACGTAACGTCCCGGCa                                                                          >  1:1027871/1‑150 (MQ=255)
                                                                              gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAg                                                                         <  1:1096511/150‑1 (MQ=255)
                                                                               tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTAGGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                        <  2:815053/150‑1 (MQ=255)
                                                                                    gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                                  >  1:1636279/1‑150 (MQ=255)
                                                                                         tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTATACGTAACGTCACGGCAGAAAAGAGCTGt                                                              >  1:493686/1‑150 (MQ=255)
                                                                                           aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                            <  1:605217/150‑1 (MQ=255)
                                                                                           aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                            >  1:540145/1‑150 (MQ=255)
                                                                                            tAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCg                                                           <  1:694824/150‑1 (MQ=255)
                                                                                              aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                         >  2:20331/1‑150 (MQ=255)
                                                                                               aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                        >  1:1085407/1‑150 (MQ=255)
                                                                                               aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                        <  2:273738/150‑1 (MQ=255)
                                                                                                   ggTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGGGGGTGGTAGCAAACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                    <  1:939979/150‑1 (MQ=255)
                                                                                                    gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAtt                                                   >  1:1748926/1‑150 (MQ=255)
                                                                                                        aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                               >  2:473357/1‑150 (MQ=255)
                                                                                                        aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                               <  2:936738/150‑1 (MQ=255)
                                                                                                          aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                             <  2:1641010/150‑1 (MQ=255)
                                                                                                          aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                             >  2:428889/1‑150 (MQ=255)
                                                                                                           ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                            <  1:1143189/150‑1 (MQ=255)
                                                                                                              gCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCtttt                                         <  1:817472/150‑1 (MQ=255)
                                                                                                               cTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttttt                                        <  1:260070/150‑1 (MQ=255)
                                                                                                                    cGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                                   >  2:101395/1‑150 (MQ=255)
                                                                                                                      ggTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACTTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                 <  2:1768463/150‑1 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTTTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                               <  2:1784170/150‑1 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                               >  2:698505/1‑150 (MQ=255)
                                                                                                                        tCTTTTTCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGCGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGATCGAGCGCATTTCTGCCTTTTTCCCGCGgcc                               >  2:1806225/1‑150 (MQ=255)
                                                                                                                            tttCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGGTAATGGGGCCTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                           <  1:336026/150‑1 (MQ=255)
                                                                                                                             ttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                          >  1:813593/1‑150 (MQ=255)
                                                                                                                                atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                       >  1:861627/1‑150 (MQ=255)
                                                                                                                                   atgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTgg                    >  2:365476/1‑150 (MQ=255)
                                                                                                                                    tgTAAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                   <  1:720755/150‑1 (MQ=255)
                                                                                                                                     gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                  <  2:803203/150‑1 (MQ=255)
                                                                                                                                     gTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                  <  2:254424/150‑1 (MQ=255)
                                                                                                                                         gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGCTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              >  2:1978455/2‑150 (MQ=255)
                                                                                                                                         gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACAACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCGGTTCGAGCGCATTTCTGGATTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              >  2:1356189/2‑150 (MQ=255)
                                                                                                                                         aTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGAAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              >  1:2042512/1‑150 (MQ=255)
                                                                                                                                         aTGCCGGGGGTTGTAGGACACCGGGCACCTCCGACCGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTTCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              <  2:978909/150‑1 (MQ=255)
                                                                                                                                          tGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGGCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCa             >  2:2148/1‑150 (MQ=255)
                                                                                                                                           gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGCAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCCGCCAAAGTGGGCAGCCag            >  1:387042/1‑149 (MQ=255)
                                                                                                                                           gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc            >  2:1817097/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           <  2:1445912/150‑1 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           >  2:660817/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTACAGCGGCCAGCCAAAGTGGGCAGCCACt           >  1:1173933/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTACCGTCCCGGCTGAAAAGAGCTGTTCGAGCGCATTTCTGGCTGTTTCAAGCGGCCAGCCAAAGTGGGCAGCCACt           >  1:546524/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           >  2:447472/1‑150 (MQ=255)
                                                                                                                                             cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGCCACtt          >  1:807272/1‑150 (MQ=255)
                                                                                                                                                 ggttgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTcc      <  2:2038915/149‑1 (MQ=255)
                                                                                                                                                     gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc  >  2:315349/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATGTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCC  >  NC_000913/2555053‑2555351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: