Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,205 G→C 7.5% intergenic (‑23/+23) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2050GC 7.5% 311.7 / 14.3 105intergenic (‑23/+23)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base G (53/43);  new base C (4/4);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

Only 100 of 111 total aligned reads displayed.

GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555056‑2555354
                                                                                                                                                     |                                                                                                                                                     
gTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGTGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                       <  1:1859573/150‑1 (MQ=255)
gTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTACGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATCGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGtagtag                                                                                                                                                       >  2:517090/1‑150 (MQ=255)
 ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                      <  1:482249/150‑1 (MQ=255)
 ttCATAGACATCCGTTGGCTGTCGTTGATGAACATTATCCAGTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                      <  1:1020570/150‑1 (MQ=255)
  tCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                                     >  1:920024/1‑150 (MQ=255)
   cATAGACATCCGTTGTCTGTCGTTGAAGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCTGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACCTGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                    >  1:204218/1‑150 (MQ=255)
       gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACAccc                                                                                                                                                <  1:1232002/150‑1 (MQ=255)
         cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                              <  2:1700758/150‑1 (MQ=255)
         cATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCgg                                                                                                                                              >  1:254424/1‑150 (MQ=255)
          aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                             <  1:1530521/150‑1 (MQ=255)
           tCCGTTGGCTGTCGTTGATGAACTTTATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTTGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                            <  2:509587/150‑1 (MQ=255)
            ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                           >  1:1624634/1‑150 (MQ=255)
             ctttGTCTGTCGGTGATGAACATTATCCACTTCGCCCTTCGGCGTGAGCTTCAGGCTTTCGGCTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                          <  1:471338/148‑1 (MQ=255)
              gTTGTCTGTCGTTGTTGATCATTATACACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGTTTCATGATAAAGATGGTGCAAATTTGCTGTACGTGTCTTTTTCATGATGTTAATGCCGGGGGTCCTACAACACCCGGCatta                                                                                                                                         <  2:1156542/150‑4 (MQ=255)
                tgtctgtcGTTGAGGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                       <  1:4830/150‑1 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      >  2:112915/1‑150 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      <  2:49956/150‑1 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCGGTCCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      <  1:390487/150‑1 (MQ=255)
                    tgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACa                                                                                                                                   >  1:693337/1‑150 (MQ=255)
                       cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                                >  1:1384849/1‑150 (MQ=255)
                        gTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGtt                                                                                                                               <  1:1336783/150‑1 (MQ=255)
                           gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                            >  2:817471/1‑150 (MQ=255)
                           gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                            >  1:1524221/1‑150 (MQ=255)
                             tgaACATTATCCACTTCGACCTTCGGCGTGCGCTTCGGGTTTTGGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGCCACCCGGCACCTCCGACAGGTTAATgg                                                                                                                          >  2:590555/1‑150 (MQ=255)
                                aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGTCACCTCCGCCACGTTAATGGGGc                                                                                                                       >  2:124944/1‑150 (MQ=255)
                                aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                       <  2:491309/150‑1 (MQ=255)
                                aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGAACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                       >  2:37248/1‑150 (MQ=255)
                                  aTTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCtt                                                                                                                     >  1:213620/1‑150 (MQ=255)
                                   ttATCCTCTTCGTCCTTCGCCGTTAGCTTGAGGTTTTCGGGTAAGGGTTCATGTTAAAGTTTGTGAAAATGGGCTGTACGGGTATTTTTAATGAGGTTAATGCCGGGTGGTGTCGGACACCCGGCACCTCCTACAGGTTAATGGGGCGTg                                                                                                                    <  2:1912072/150‑1 (MQ=255)
                                   ttATCCACTTCGTCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  1:1093527/150‑1 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  2:609999/150‑1 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGGGAGCTGCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  2:1386037/150‑1 (MQ=255)
                                    tATCCACTTCGTACTTCGTCGTTAGCTTCTGGTTTTCGTGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                   <  2:1056897/150‑1 (MQ=255)
                                    tATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                   <  2:1113373/150‑1 (MQ=255)
                                      tCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGATTgaga                                                                                                                 >  1:402621/1‑150 (MQ=255)
                                      tCCACTTCGACCTTCGGCGTGAGCTTAAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATTCCGGGTGTTGTAGGACACACGGCACCTCCGACAGGTTAATTGGGCTTgaga                                                                                                                 >  1:1720931/1‑150 (MQ=255)
                                       ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                                >  2:1996901/1‑150 (MQ=255)
                                         aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                              >  2:939978/1‑150 (MQ=255)
                                          cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                             >  1:486222/1‑150 (MQ=255)
                                             cGACCTTCGGCGTTAGGTTCAGGTTTTCGGGTAAGGGGTCATGATAACGATGGTGCAATTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAAc                                                                                                          <  1:992822/150‑1 (MQ=255)
                                                     ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                                  >  2:260070/1‑150 (MQ=255)
                                                     ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                                  >  1:1641011/1‑150 (MQ=255)
                                                         tGAGCTTCTGTTTTTCGGTTAAGGGTTCTTCAAAACGATGGTGCAAATTGGCTGTACGGGTCTTTTACATCTTGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGtt                                                                                              <  2:1505088/150‑1 (MQ=255)
                                                            gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTAc                                                                                           <  2:150995/150‑1 (MQ=255)
                                                             gttatggTTTTCGGTTAATGGTTAATTATAAATATGTTACAAATTGGCTGTTCGGGGTTTTTTCTTGTTTTTATTCCGGGTTGTTGTCTGCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACTATAACGACTACTGCGTTTACg                                                                                          <  2:696691/145‑1 (MQ=255)
                                                             cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                          >  1:17528/1‑150 (MQ=255)
                                                             cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATCGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGTTGTTGTAGGACACACGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAACTACAAAAGCGTTTACg                                                                                          >  1:640737/1‑150 (MQ=255)
                                                               tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                        <  1:1606708/150‑1 (MQ=255)
                                                               tCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                        <  2:15879/150‑1 (MQ=255)
                                                                  ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACg                                                                                     >  1:1165899/1‑150 (MQ=255)
                                                                          ggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGTTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTACGTAACGTCCCGGCa                                                                             >  1:1027871/1‑150 (MQ=255)
                                                                           gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAg                                                                            <  1:1096511/150‑1 (MQ=255)
                                                                            tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTAGGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                           <  2:815053/150‑1 (MQ=255)
                                                                                 gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                                     >  1:1636279/1‑150 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTATACGTAACGTCACGGCAGAAAAGAGCTGt                                                                 >  1:493686/1‑150 (MQ=255)
                                                                                        aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                               >  1:540145/1‑150 (MQ=255)
                                                                                        aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                               <  1:605217/150‑1 (MQ=255)
                                                                                         tAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCg                                                              <  1:694824/150‑1 (MQ=255)
                                                                                           aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                            >  2:20331/1‑150 (MQ=255)
                                                                                            aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                           >  1:1085407/1‑150 (MQ=255)
                                                                                            aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                           <  2:273738/150‑1 (MQ=255)
                                                                                                ggTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGGGGGTGGTAGCAAACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                       <  1:939979/150‑1 (MQ=255)
                                                                                                 gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAtt                                                      >  1:1748926/1‑150 (MQ=255)
                                                                                                     aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                  >  2:473357/1‑150 (MQ=255)
                                                                                                     aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                  <  2:936738/150‑1 (MQ=255)
                                                                                                       aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                                <  2:1641010/150‑1 (MQ=255)
                                                                                                        ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                               <  1:1143189/150‑1 (MQ=255)
                                                                                                           gCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCtttt                                            <  1:817472/150‑1 (MQ=255)
                                                                                                            cTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttttt                                           <  1:260070/150‑1 (MQ=255)
                                                                                                                 cGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                                      >  2:101395/1‑150 (MQ=255)
                                                                                                                   ggTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACTTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                    <  2:1768463/150‑1 (MQ=255)
                                                                                                                     tCTTTTTCATGATGTTAATGCCGGGTGTTTTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                                  <  2:1784170/150‑1 (MQ=255)
                                                                                                                     tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                                  >  2:698505/1‑150 (MQ=255)
                                                                                                                     tCTTTTTCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGCGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGATCGAGCGCATTTCTGCCTTTTTCCCGCGgcc                                  >  2:1806225/1‑150 (MQ=255)
                                                                                                                     gcTTTTTCATGAAGTTATTGCCGGGTGTTGGAGGACAGCCGGCTACTCCGTCAGGTTAATGGGGCTTGAGTCGATAACGACTACTGCGTTTACGTTACGTCCCGGCAGTATAGAGCTGTTCGAGCGGATTTCTGGCTTTTTCGAGCGgcc                                  <  2:357100/149‑1 (MQ=255)
                                                                                                                         tttCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGGTAATGGGGCCTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                              <  1:336026/150‑1 (MQ=255)
                                                                                                                          ttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                             >  1:813593/1‑150 (MQ=255)
                                                                                                                             atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                          >  1:861627/1‑150 (MQ=255)
                                                                                                                                atgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTgg                       >  2:365476/1‑150 (MQ=255)
                                                                                                                                 tgTAAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                      <  1:720755/150‑1 (MQ=255)
                                                                                                                                  gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                     <  2:803203/150‑1 (MQ=255)
                                                                                                                                  gTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                     <  2:254424/150‑1 (MQ=255)
                                                                                                                                      gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGCTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 >  2:1978455/2‑150 (MQ=255)
                                                                                                                                      gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACAACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCGGTTCGAGCGCATTTCTGGATTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 >  2:1356189/2‑150 (MQ=255)
                                                                                                                                      aTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGAAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 >  1:2042512/1‑150 (MQ=255)
                                                                                                                                      aTGCCGGGGGTTGTAGGACACCGGGCACCTCCGACCGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTTCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 <  2:978909/150‑1 (MQ=255)
                                                                                                                                       tGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGGCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCa                >  2:2148/1‑150 (MQ=255)
                                                                                                                                        gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc               >  2:1817097/1‑150 (MQ=255)
                                                                                                                                         ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              <  2:1445912/150‑1 (MQ=255)
                                                                                                                                         ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              >  2:660817/1‑150 (MQ=255)
                                                                                                                                         ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTACAGCGGCCAGCCAAAGTGGGCAGCCACt              >  1:1173933/1‑150 (MQ=255)
                                                                                                                                         ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTACCGTCCCGGCTGAAAAGAGCTGTTCGAGCGCATTTCTGGCTGTTTCAAGCGGCCAGCCAAAGTGGGCAGCCACt              >  1:546524/1‑150 (MQ=255)
                                                                                                                                         ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              >  2:447472/1‑150 (MQ=255)
                                                                                                                                          cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGCCACtt             >  1:807272/1‑150 (MQ=255)
                                                                                                                                                tggtAGGACACCCGGCACCTCCGACTGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTg       <  2:1868018/148‑1 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc     >  2:315349/1‑150 (MQ=255)
                                                                                                                                                   tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg    >  1:1681586/1‑150 (MQ=255)
                                                                                                                                                    aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGt   >  2:1730490/1‑150 (MQ=255)
                                                                                                                                                     ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  <  2:402621/150‑1 (MQ=255)
                                                                                                                                                     ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAATAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCTAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  <  2:565019/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555056‑2555354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: