Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,206 G→A 8.8% intergenic (‑24/+22) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2060GA 8.8% 295.9 / 13.5 105intergenic (‑24/+22)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base G (52/43);  new base A (5/4);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 109 total aligned reads displayed.

TTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555057‑2555354
                                                                                                                                                     |                                                                                                                                                    
ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                      <  1:482249/150‑1 (MQ=255)
ttCATAGACATCCGTTGGCTGTCGTTGATGAACATTATCCAGTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                      <  1:1020570/150‑1 (MQ=255)
 tCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                                     >  1:920024/1‑150 (MQ=255)
  cATAGACATCCGTTGTCTGTCGTTGAAGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCTGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACCTGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                    >  1:204218/1‑150 (MQ=255)
      gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACAccc                                                                                                                                                <  1:1232002/150‑1 (MQ=255)
       aCATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                              <  2:639427/150‑1 (MQ=255)
        cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                              <  2:1700758/150‑1 (MQ=255)
        cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                              <  1:1740114/150‑1 (MQ=255)
        cATCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCgg                                                                                                                                              >  1:254424/1‑150 (MQ=255)
         aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                             <  1:1530521/150‑1 (MQ=255)
          tCCGTTGGCTGTCGTTGATGAACTTTATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTTGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                            <  2:509587/150‑1 (MQ=255)
           ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                           >  1:1624634/1‑150 (MQ=255)
            ctttGTCTGTCGGTGATGAACATTATCCACTTCGCCCTTCGGCGTGAGCTTCAGGCTTTCGGCTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                          <  1:471338/148‑1 (MQ=255)
            cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                          >  1:565019/1‑150 (MQ=255)
             gTTGTCTGTCGTTGTTGATCATTATACACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGTTTCATGATAAAGATGGTGCAAATTTGCTGTACGTGTCTTTTTCATGATGTTAATGCCGGGGGTCCTACAACACCCGGCatta                                                                                                                                         <  2:1156542/150‑4 (MQ=255)
               tgtctgtcGTTGAGGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                       <  1:4830/150‑1 (MQ=255)
                gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      >  2:112915/1‑150 (MQ=255)
                gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      <  2:49956/150‑1 (MQ=255)
                gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCGGTCCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      <  1:390487/150‑1 (MQ=255)
                   tgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACa                                                                                                                                   >  1:693337/1‑150 (MQ=255)
                      cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                                >  1:1384849/1‑150 (MQ=255)
                       gTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGtt                                                                                                                               <  1:1336783/150‑1 (MQ=255)
                          gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                            >  2:1744424/1‑150 (MQ=255)
                          gatgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                            >  2:817471/1‑150 (MQ=255)
                               aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGTGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGTCACCTCCGCCACGTTAATGGGGc                                                                                                                       >  2:124944/1‑150 (MQ=255)
                               aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                       <  2:491309/150‑1 (MQ=255)
                               aCATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGAACACCCGGCACCTCCGACAGGTTAATGGGGc                                                                                                                       >  2:37248/1‑150 (MQ=255)
                                 aTTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCtt                                                                                                                     >  1:213620/1‑150 (MQ=255)
                                  ttATCCTCTTCGTCCTTCGCCGTTAGCTTGAGGTTTTCGGGTAAGGGTTCATGTTAAAGTTTGTGAAAATGGGCTGTACGGGTATTTTTAATGAGGTTAATGCCGGGTGGTGTCGGACACCCGGCACCTCCTACAGGTTAATGGGGCGTg                                                                                                                    <  2:1912072/150‑1 (MQ=255)
                                  ttATCCACTTCGTCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  1:1093527/150‑1 (MQ=255)
                                  ttATCCACTTCGACCTTCGGCGGGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  2:609999/150‑1 (MQ=255)
                                  ttATCCACTTCGACCTTCGGCGGGAGCTGCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  2:1386037/150‑1 (MQ=255)
                                   tATCCACTTCGTACTTCGTCGTTAGCTTCTGGTTTTCGTGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                   <  2:1056897/150‑1 (MQ=255)
                                   tATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                   <  2:1113373/150‑1 (MQ=255)
                                     tCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGATTgaga                                                                                                                 >  1:402621/1‑150 (MQ=255)
                                     tCCACTTCGACCTTCGGCGTGAGCTTAAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATTCCGGGTGTTGTAGGACACACGGCACCTCCGACAGGTTAATTGGGCTTgaga                                                                                                                 >  1:1720931/1‑150 (MQ=255)
                                      ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                                >  2:1996901/1‑150 (MQ=255)
                                        aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                              >  2:939978/1‑150 (MQ=255)
                                         cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                             >  1:486222/1‑150 (MQ=255)
                                            cGACCTTCGGCGTTAGGTTCAGGTTTTCGGGTAAGGGGTCATGATAACGATGGTGCAATTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAAc                                                                                                          <  1:992822/150‑1 (MQ=255)
                                                    ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                                  >  1:1641011/1‑150 (MQ=255)
                                                    ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                                  >  2:260070/1‑150 (MQ=255)
                                                        tGAGCTTCTGTTTTTCGGTTAAGGGTTCTTCAAAACGATGGTGCAAATTGGCTGTACGGGTCTTTTACATCTTGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGtt                                                                                              <  2:1505088/150‑1 (MQ=255)
                                                           gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTAc                                                                                           <  2:150995/150‑1 (MQ=255)
                                                           gCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTAc                                                                                           >  2:125571/1‑150 (MQ=255)
                                                            gttatggTTTTCGGTTAATGGTTAATTATAAATATGTTACAAATTGGCTGTTCGGGGTTTTTTCTTGTTTTTATTCCGGGTTGTTGTCTGCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACTATAACGACTACTGCGTTTACg                                                                                          <  2:696691/145‑1 (MQ=255)
                                                            cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                          >  1:17528/1‑150 (MQ=255)
                                                            cTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATCGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGTTGTTGTAGGACACACGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAACTACAAAAGCGTTTACg                                                                                          >  1:640737/1‑150 (MQ=255)
                                                              tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                        <  1:893787/150‑1 (MQ=255)
                                                              tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                        <  1:1606708/150‑1 (MQ=255)
                                                              tCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                        <  2:15879/150‑1 (MQ=255)
                                                                 ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACg                                                                                     >  1:1165899/1‑150 (MQ=255)
                                                                    tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                                  >  1:1372590/1‑150 (MQ=255)
                                                                         ggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGTTTAATGGGGCTTGAGACGATAACGACTACTGAGTTTACGTAACGTCCCGGCa                                                                             >  1:1027871/1‑150 (MQ=255)
                                                                          gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAg                                                                            <  1:1096511/150‑1 (MQ=255)
                                                                           tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTAGGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCGGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                           <  2:815053/150‑1 (MQ=255)
                                                                                gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                                     >  1:1636279/1‑150 (MQ=255)
                                                                                       aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                               >  1:540145/1‑150 (MQ=255)
                                                                                       aTAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTc                                                               <  1:605217/150‑1 (MQ=255)
                                                                                        tAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCg                                                              <  1:694824/150‑1 (MQ=255)
                                                                                          aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                            >  2:20331/1‑150 (MQ=255)
                                                                                           aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                           >  1:1085407/1‑150 (MQ=255)
                                                                                           aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                           <  2:273738/150‑1 (MQ=255)
                                                                                               ggTGCAAATTGGCTGTCCGGGTCTTTTTCATGATGTTAATGCCGGGGGTGGTAGCAAACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                       <  1:939979/150‑1 (MQ=255)
                                                                                                gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAtt                                                      >  1:1748926/1‑150 (MQ=255)
                                                                                                    aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                  >  2:473357/1‑150 (MQ=255)
                                                                                                    aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                  <  2:936738/150‑1 (MQ=255)
                                                                                                      aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGTGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                                <  2:1641010/150‑1 (MQ=255)
                                                                                                      aTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                                >  2:428889/1‑150 (MQ=255)
                                                                                                       ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                               <  1:1143189/150‑1 (MQ=255)
                                                                                                          gCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCtttt                                            <  1:817472/150‑1 (MQ=255)
                                                                                                           cTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttttt                                           <  1:260070/150‑1 (MQ=255)
                                                                                                                cGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGCCTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                                      >  2:101395/1‑150 (MQ=255)
                                                                                                                  ggTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACTTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                    <  2:1768463/150‑1 (MQ=255)
                                                                                                                    tCTTTTTCATGATGTTAATGCCGGGTGTTTTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                                  <  2:1784170/150‑1 (MQ=255)
                                                                                                                    tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                                  >  2:698505/1‑150 (MQ=255)
                                                                                                                    gcTTTTTCATGAAGTTATTGCCGGGTGTTGGAGGACAGCCGGCTACTCCGTCAGGTTAATGGGGCTTGAGTCGATAACGACTACTGCGTTTACGTTACGTCCCGGCAGTATAGAGCTGTTCGAGCGGATTTCTGGCTTTTTCGAGCGgcc                                  <  2:357100/149‑1 (MQ=255)
                                                                                                                        tttCATGATGTTAATGCCGGGTGTTCTAGGACACCCGGCACCTCCGACAGGGTAATGGGGCCTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                              <  1:336026/150‑1 (MQ=255)
                                                                                                                         ttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                             >  1:813593/1‑150 (MQ=255)
                                                                                                                               atgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTgg                       >  2:365476/1‑150 (MQ=255)
                                                                                                                                tgTAAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                      <  1:720755/150‑1 (MQ=255)
                                                                                                                                 gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                     <  2:803203/150‑1 (MQ=255)
                                                                                                                                 gTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGc                     <  2:254424/150‑1 (MQ=255)
                                                                                                                                     gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGCTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 >  2:1978455/2‑150 (MQ=255)
                                                                                                                                     aTGCCGGGGGTTGTAGGACACCGGGCACCTCCGACCGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTTCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 <  2:978909/150‑1 (MQ=255)
                                                                                                                                      tGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGGCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCa                >  2:2148/1‑150 (MQ=255)
                                                                                                                                       gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGCAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCCGCCAAAGTGGGCAGCCag               >  1:387042/1‑149 (MQ=255)
                                                                                                                                       gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc               >  2:1817097/1‑150 (MQ=255)
                                                                                                                                        ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              >  2:660817/1‑150 (MQ=255)
                                                                                                                                        ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              <  2:1445912/150‑1 (MQ=255)
                                                                                                                                        ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTACAGCGGCCAGCCAAAGTGGGCAGCCACt              >  1:1173933/1‑150 (MQ=255)
                                                                                                                                        ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTACCGTCCCGGCTGAAAAGAGCTGTTCGAGCGCATTTCTGGCTGTTTCAAGCGGCCAGCCAAAGTGGGCAGCCACt              >  1:546524/1‑150 (MQ=255)
                                                                                                                                        ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              >  2:447472/1‑150 (MQ=255)
                                                                                                                                         cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGCCACtt             >  1:807272/1‑150 (MQ=255)
                                                                                                                                             ggttgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTcc         <  2:2038915/149‑1 (MQ=255)
                                                                                                                                               tggtAGGACACCCGGCACCTCCGACTGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTg       <  2:1868018/148‑1 (MQ=255)
                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg    >  1:1681586/1‑150 (MQ=255)
                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGt   >  2:1730490/1‑150 (MQ=255)
                                                                                                                                                    ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAATGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  <  2:402621/150‑1 (MQ=255)
                                                                                                                                                    ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAATAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCTAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  <  2:565019/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555057‑2555354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: