Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,367 G→A 5.2% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3670GA 5.2% 362.7 / 5.4 115intergenic (+28/‑488)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (56/52);  new base A (4/2);  total (60/55)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 125 total aligned reads displayed.

TAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTA  >  NC_000913/2653218‑2653514
                                                                                                                                                     |                                                                                                                                                   
tAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAccgccg                                                                                                                                                     >  1:807030/1‑150 (MQ=255)
      cAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                                               <  2:1769812/150‑1 (MQ=255)
       aGCCTTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTgg                                                                                                                                              <  2:1369131/150‑1 (MQ=255)
       aGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTgg                                                                                                                                              >  1:880122/1‑150 (MQ=255)
        gCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGc                                                                                                                                             >  2:1869622/1‑150 (MQ=255)
         ccGGGGTTTAGTTAGCACTCGCGACGCTGGATGTCCCACACGTGCAACTGTCCGACGGCACATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGTTTGAGGCAGTGTAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCg                                                                                                                                            <  2:1098945/150‑1 (MQ=255)
          cGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCgg                                                                                                                                           >  1:1011310/1‑150 (MQ=255)
              gTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttt                                                                                                                                       <  2:719715/150‑1 (MQ=255)
               ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGttttt                                                                                                                                      <  1:585523/150‑1 (MQ=255)
               ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTAACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGttttt                                                                                                                                      <  2:1711165/150‑1 (MQ=255)
                    ttcgCACTCGCGACGCTGGATGTGCCAAACGGGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTg                                                                                                                                 <  2:404265/147‑1 (MQ=255)
                         aCTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGt                                                                                                                            <  2:229507/150‑1 (MQ=255)
                          cTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTc                                                                                                                           >  2:828881/1‑150 (MQ=255)
                          cTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTc                                                                                                                           >  2:1742203/1‑150 (MQ=255)
                            cgcgACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTg                                                                                                                         <  2:757412/150‑1 (MQ=255)
                            cgcgACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTg                                                                                                                         >  1:873938/1‑150 (MQ=255)
                                aCGCTGGATGGGCCAAACGTGAAACGGTACGACGGCGCAGGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTc                                                                                                                     <  2:596736/150‑1 (MQ=255)
                                     ggATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGc                                                                                                                <  2:223131/150‑1 (MQ=255)
                                     ggATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGc                                                                                                                >  1:1777503/1‑150 (MQ=255)
                                        tgtgCCAAACGTGAAACTGTACGAGGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCtt                                                                                                             <  1:1037833/150‑1 (MQ=255)
                                          tgCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGTTTCGCGGCCTTTc                                                                                                           >  1:226485/1‑150 (MQ=255)
                                          tgCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                           >  1:916838/1‑150 (MQ=255)
                                           gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTcc                                                                                                          <  2:1792615/150‑1 (MQ=255)
                                           gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTGTTATTGCTAGTCTGGTTCGCGGCCTTTcc                                                                                                          >  2:1531856/1‑150 (MQ=255)
                                             cAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                                                                        >  1:1065178/1‑150 (MQ=255)
                                               aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagca                                                                                                      <  1:1696238/150‑1 (MQ=255)
                                                 cGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAgg                                                                                                    >  2:368514/1‑150 (MQ=255)
                                                 cGTGAAACAGTACGACGGCGCATGGAGTTAACGGCGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCATAAAAAAACCGCCGAATTTGTCGGTTTTTTATTGCTAGTCTGGTTCTCGGCCTTTCCAGCAgg                                                                                                    <  2:1761170/150‑1 (MQ=255)
                                                   tGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCCGCAGGtt                                                                                                  >  2:1470721/1‑150 (MQ=255)
                                                   tGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                  >  1:55446/1‑150 (MQ=255)
                                                   tGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGATTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAACAgggt                                                                                                  >  2:1125297/1‑148 (MQ=255)
                                                    gAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTg                                                                                                 >  2:1459138/1‑150 (MQ=255)
                                                     aaaCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGa                                                                                                >  2:2003791/1‑150 (MQ=255)
                                                      aaCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGAc                                                                                               <  2:535820/150‑1 (MQ=255)
                                                      aaCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGAGAAATAAGTATTTTACAGTCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCCCGGCCTTTCCAGCAGGTAGAc                                                                                               >  1:1614624/1‑150 (MQ=255)
                                                       aCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACt                                                                                              <  2:1403742/150‑1 (MQ=255)
                                                       aCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACt                                                                                              <  2:596601/150‑1 (MQ=255)
                                                           taagacgTCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgtg                                                                                          <  1:384361/147‑1 (MQ=255)
                                                             cgacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGGCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTGCAGCAGGTTGACTTGTCtt                                                                                        >  2:1153934/1‑150 (MQ=255)
                                                              gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                                                                       <  2:1864409/150‑1 (MQ=255)
                                                              gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                                                                       >  2:615349/1‑150 (MQ=255)
                                                              gacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCAGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                                                                       <  2:730672/150‑1 (MQ=255)
                                                                cgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                                                                     <  1:265664/150‑1 (MQ=255)
                                                                  gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATg                                                                                   <  2:419948/150‑1 (MQ=255)
                                                                  gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATg                                                                                   >  2:353065/1‑150 (MQ=255)
                                                                   cgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATGAGTATTTTACAGGCAATAAAAAACCGCAGATTTTGGCGGTTTTTTATGGCTAGTCTGGTTCGCGGCGTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                  >  2:1921280/1‑150 (MQ=255)
                                                                   cgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGa                                                                                  >  2:1893031/1‑150 (MQ=255)
                                                                     cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGc                                                                                >  2:877892/1‑150 (MQ=255)
                                                                          tgTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGc                                                                           <  2:1342078/149‑1 (MQ=255)
                                                                           gTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                                                                          <  1:638615/150‑1 (MQ=255)
                                                                            gtcctGGGGCGAGAGGGCATATTTACCGGTTGCGCCAGTGAAATAAGTATTTTACAGGAAATAAAAAACCTCCTAATTTGGCGGTTTTTTATTGATAGTCTGGTTCTCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                                         <  2:1577658/146‑1 (MQ=255)
                                                                             gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                        >  1:237970/1‑150 (MQ=255)
                                                                             gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                        >  1:1059537/1‑150 (MQ=255)
                                                                             gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                        <  1:743922/150‑1 (MQ=255)
                                                                                  gggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCtt                                                                   >  2:629727/1‑150 (MQ=255)
                                                                                   ggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTc                                                                  >  1:1390825/1‑150 (MQ=255)
                                                                                            gCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCCTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaaa                                                         >  1:1204518/1‑150 (MQ=255)
                                                                                               gATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACaa                                                      >  1:736881/1‑150 (MQ=255)
                                                                                               gATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACaa                                                      >  1:62007/1‑150 (MQ=255)
                                                                                                 tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATa                                                    >  1:1372653/1‑150 (MQ=255)
                                                                                                      cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACGATACTCAc                                               >  2:1146800/1‑150 (MQ=255)
                                                                                                      cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                               <  1:481648/150‑1 (MQ=255)
                                                                                                               cAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActc                                      >  2:1382194/1‑150 (MQ=255)
                                                                                                               cAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActc                                      <  2:1455748/150‑1 (MQ=255)
                                                                                                                aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActct                                     <  1:1859804/150‑1 (MQ=255)
                                                                                                                 gTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActctc                                    >  2:1491009/1‑150 (MQ=255)
                                                                                                                     aaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCATCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                <  1:1488000/150‑1 (MQ=255)
                                                                                                                       tAATTATTTTACAGGAAATAACAACCCGCCGAAGTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCTGCCTTTCCAGCAGGTTGACTTGTGTTAGATGAGCAACGCAGGTTCTTCACATCAAAACAATACTCACCAGTAACTCTCTTTTTg                              <  2:185011/150‑1 (MQ=255)
                                                                                                                       tAAGTATTTTACAGGCAATAACAAACCGCCGACTTTGGCGGTTTTTTATTGCTAGTCTGGGTCGCGGCCTTTCCCGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTg                              <  1:1328552/150‑1 (MQ=255)
                                                                                                                       tAAGTATTTTACAGGCAATAAAATACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTg                              <  2:1605608/150‑1 (MQ=255)
                                                                                                                         aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTTCATGAGCAACGCAGGTGCTTCACAGCAATACAATACTCACCAGTAACTCTCTTTTTGtt                            >  2:974279/1‑149 (MQ=255)
                                                                                                                         aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTc                            >  2:1178032/1‑150 (MQ=255)
                                                                                                                         aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTc                            <  2:194328/150‑1 (MQ=255)
                                                                                                                                 aCAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCaaaa                    <  1:629727/150‑1 (MQ=255)
                                                                                                                                  cAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCACCGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAag                   >  1:1190617/1‑150 (MQ=255)
                                                                                                                                    ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCGAGCAAAagag                 <  2:1011309/150‑1 (MQ=255)
                                                                                                                                    ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagag                 >  2:1989511/1‑150 (MQ=255)
                                                                                                                                    ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagag                 <  1:1869623/150‑1 (MQ=255)
                                                                                                                                     gCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagaga                <  1:9009/150‑1 (MQ=255)
                                                                                                                                      cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagagag               >  2:548247/1‑150 (MQ=255)
                                                                                                                                      cAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGACTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAagagag               <  1:262138/150‑1 (MQ=255)
                                                                                                                                       aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGGAAAACAATACTCAGCACTAACTCTCTTTTTGTCAAGCAAAAGAGAGt              <  2:916837/150‑1 (MQ=255)
                                                                                                                                       aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt              >  2:1502564/1‑150 (MQ=255)
                                                                                                                                       aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt              >  1:1070943/1‑150 (MQ=255)
                                                                                                                                        aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTa             <  1:1742204/150‑1 (MQ=255)
                                                                                                                                        aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTa             >  1:708608/1‑150 (MQ=255)
                                                                                                                                        aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTa             >  1:1208054/1‑150 (MQ=255)
                                                                                                                                        aTAAAAAACCGCCAAATTAGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCATAAGAGAGTa             >  2:192246/1‑150 (MQ=255)
                                                                                                                                         tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCAGGTTCGCGGCCTTTCCATCAGCTTGAATTTTGTTAAAAGAGAAAAGAAGGTTATTAACAGCAAAACAATACTAACATTTAAATCTAATTTTGTCATGGAAAGGAGAGTat            >  2:1357157/1‑149 (MQ=255)
                                                                                                                                           aaaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatt          >  2:772117/1‑150 (MQ=255)
                                                                                                                                            aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAatta         >  2:648891/1‑150 (MQ=255)
                                                                                                                                              aaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGGGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAattatt       <  2:1857142/150‑1 (MQ=255)
                                                                                                                                                 cgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtt    <  2:644300/150‑1 (MQ=255)
                                                                                                                                                 cgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtt    <  2:1441082/150‑1 (MQ=255)
                                                                                                                                                  gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttt   >  2:1514196/1‑150 (MQ=255)
                                                                                                                                                  gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttt   >  1:519194/1‑150 (MQ=255)
                                                                                                                                                  gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttt   <  2:1060330/150‑1 (MQ=255)
                                                                                                                                                  gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGAAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttt   <  2:1614623/150‑1 (MQ=255)
                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttta  >  1:1320341/1‑150 (MQ=255)
                                                                                                                                                   ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGatta  >  1:1777250/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTA  >  NC_000913/2653218‑2653514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: