Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,707,320 A→T 6.7% intergenic (‑196/+2) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,707,3200AT 6.7% 339.4 / 10.8 119intergenic (‑196/+2)lepA/rseCback‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base A (50/61);  new base T (2/6);  total (52/67)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 120 total aligned reads displayed.

AGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGA  >  NC_000913/2707172‑2707467
                                                                                                                                                    |                                                                                                                                                   
aGTCGCTTTTCTGAGCTTAACGTCTGTATAAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGTTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaaa                                                                                                                                                    <  2:607166/150‑1 (MQ=255)
  tCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                                                                  >  2:1176884/1‑150 (MQ=255)
  tCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                                                                  <  1:1745589/150‑1 (MQ=255)
     cTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACt                                                                                                                                               <  2:1579434/150‑1 (MQ=255)
       gTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTgg                                                                                                                                             >  1:809856/1‑150 (MQ=255)
         tCTGAGCTTAACGTCTGTATTAATAGAACCGCCGCATTCTACACTACAACATTTAGGCGAGGAAATGTTCATACCGTATGTATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCt                                                                                                                                           <  1:807487/150‑1 (MQ=255)
         tCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCt                                                                                                                                           <  2:452603/150‑1 (MQ=255)
           tGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                                                                         <  2:209748/150‑3 (MQ=255)
           tGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcg                                                                                                                                         <  1:1574585/150‑1 (MQ=255)
           ggAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                                                                         <  1:1991011/149‑3 (MQ=255)
            gAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttg                                                                                                                                        <  1:1814737/150‑4 (MQ=255)
              tgttAACGTCTGTATAAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATAACGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGt                                                                                                                                      <  1:991401/148‑1 (MQ=255)
              gCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgtt                                                                                                                                      <  2:1839648/150‑6 (MQ=255)
               cTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACTACATTGAGTCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                                                                     <  2:534607/150‑1 (MQ=255)
               cTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                                                                     <  2:938557/150‑1 (MQ=255)
                  aaCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTGATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTc                                                                                                                                  <  2:362218/150‑1 (MQ=255)
                   aCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                                                                 >  1:1740552/1‑150 (MQ=255)
                   aCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGCCTCGCGTCTTcc                                                                                                                                 >  2:1570879/1‑150 (MQ=255)
                    cGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCg                                                                                                                                >  2:667123/1‑150 (MQ=255)
                       cTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAg                                                                                                                             <  2:561834/150‑1 (MQ=255)
                        tgaaTTAATAGAAACGCCGCATTCTAGACTACAACAATCAGGCGAGTAAATGTTAATACATTAAGGATTGTTGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                                                                            <  1:101230/147‑1 (MQ=255)
                        tGTATTAATAGAAACGCCGCATTCTACACTACAACATTGATGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                                                                            <  1:1343174/150‑1 (MQ=255)
                         gTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGAt                                                                                                                           >  1:604604/1‑150 (MQ=255)
                         gTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGAt                                                                                                                           >  2:930326/1‑150 (MQ=255)
                          tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATg                                                                                                                          <  1:1438672/150‑1 (MQ=255)
                          tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCAGAAGAtt                                                                                                                          >  2:1689591/1‑149 (MQ=255)
                           aTTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGt                                                                                                                         <  2:587352/150‑1 (MQ=255)
                              aaTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTc                                                                                                                      <  2:1636322/150‑1 (MQ=255)
                              aaTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTc                                                                                                                      <  1:1445020/150‑1 (MQ=255)
                               aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCa                                                                                                                     <  1:1135890/150‑1 (MQ=255)
                                tAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTACGAAGATGATTCaa                                                                                                                    >  1:718197/1‑150 (MQ=255)
                                 aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTAATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGAAAAATGCAACAAAGCCAGTGAAATCAATGTCTCGCGTCTTCCGAAGATGTTTCaaa                                                                                                                   >  1:1240488/1‑150 (MQ=255)
                                    aaCGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCCCCGGCTCGCGTCTTCCGAAGATGTTTCAAATCg                                                                                                                >  2:1585240/1‑150 (MQ=255)
                                        ccgcATTCTACACTACAACATTGAGGCGAGGTAATGTTCATACCGTATGGATTGTGGTATCTGGAACCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCACGCGTCTTCCGAAGATGTTGGAAATCGcaca                                                                                                            >  2:1712378/1‑150 (MQ=255)
                                          gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAg                                                                                                          <  1:826010/150‑1 (MQ=255)
                                            aTTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGc                                                                                                        <  1:250588/150‑1 (MQ=255)
                                             ttCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                                                                       <  1:1849536/150‑1 (MQ=255)
                                             ttCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGAATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                                                                       <  2:141704/150‑1 (MQ=255)
                                              tCTACACTACAACATTGAGGGGAGGAAATGTTCATACCGTATGGATTGTGGTAGCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCTCTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACATGGCCa                                                                                                      <  2:313839/150‑1 (MQ=255)
                                              tCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCa                                                                                                      <  1:634363/150‑1 (MQ=255)
                                                  cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcg                                                                                                  >  1:1048059/1‑150 (MQ=255)
                                                    ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAtgcggc                                                                                                >  1:403504/1‑150 (MQ=255)
                                                    ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcggc                                                                                                >  2:1711367/1‑150 (MQ=255)
                                                    ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcggc                                                                                                <  1:1939060/150‑1 (MQ=255)
                                                      acaacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCaa                                                                                              >  1:48197/1‑150 (MQ=255)
                                                            ttGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                                                                        <  1:723416/150‑1 (MQ=255)
                                                              gAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGc                                                                                      >  1:1026123/1‑150 (MQ=255)
                                                               aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCt                                                                                     >  2:636228/1‑150 (MQ=255)
                                                                 gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTa                                                                                   >  2:841403/1‑150 (MQ=255)
                                                                  cGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATTCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTaa                                                                                  >  1:1228795/1‑150 (MQ=255)
                                                                         aTGTTCATACCGTATTGATTGGGGTATCTGGTATCGTCCTCGCTTTTGTTATTCAAAATGAAACAAAGCCAGTGAAATGACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATc                                                                           <  2:409802/150‑1 (MQ=255)
                                                                              cATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTg                                                                      >  1:1874103/1‑150 (MQ=255)
                                                                              aaTACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTg                                                                      <  1:53940/149‑1 (MQ=255)
                                                                               aTACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGc                                                                     <  1:1528822/150‑1 (MQ=255)
                                                                                 aCCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCa                                                                   <  2:189311/150‑1 (MQ=255)
                                                                                    gTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTc                                                                <  1:1186508/150‑1 (MQ=255)
                                                                                       tGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc                                                             >  2:1832376/1‑150 (MQ=255)
                                                                                        ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc                                                            <  2:1740551/150‑1 (MQ=255)
                                                                                        ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc                                                            >  2:842063/1‑150 (MQ=255)
                                                                                        ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTAAGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc                                                            <  2:1083761/150‑1 (MQ=255)
                                                                                        ggATTGTGGTATCTGGAAACGGCCTCGCATGTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGcc                                                            <  2:122017/150‑1 (MQ=255)
                                                                                         gATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCCCGCTTAAGATGATCGGTTGCCATTATGccc                                                           >  2:1090529/1‑150 (MQ=255)
                                                                                         gATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGccc                                                           <  2:824234/150‑1 (MQ=255)
                                                                                          aTTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg                                                          <  1:1299451/150‑1 (MQ=255)
                                                                                             gtgGTATCTGGAAACGTCCTCGCATGTGTTATGCAAAATGCAACAAAGCCAGGGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCTGGCGGCAATGCCACGCTTAAGATGATCGGGTGCAATTCTGCCCGGgc                                                       >  2:345017/1‑150 (MQ=255)
                                                                                               ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATAGCACAGGGCCCGGCGGCAATGCCCCGCGGAAGCTGATCGGTTGCCCTTCTGCCCggggcgc                                                    >  2:359454/1‑146 (MQ=255)
                                                                                                   ccTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCaaa                                                 >  2:1475150/2‑150 (MQ=255)
                                                                                                    cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAAc                                                <  2:1139085/150‑1 (MQ=255)
                                                                                                      ggAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACtt                                              >  1:1210039/1‑150 (MQ=255)
                                                                                                        aaaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAc                                            >  2:1920525/1‑150 (MQ=255)
                                                                                                          aCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgc                                          <  1:565124/150‑1 (MQ=255)
                                                                                                           cGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGACGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgcg                                         <  1:318145/150‑1 (MQ=255)
                                                                                                           cGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgcg                                         <  1:1554609/150‑1 (MQ=255)
                                                                                                           cGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTAcgcg                                         <  2:1452439/150‑1 (MQ=255)
                                                                                                               cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTa                                     <  1:1689592/150‑1 (MQ=255)
                                                                                                                tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAg                                    >  2:1779085/1‑150 (MQ=255)
                                                                                                                   cATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcg                                 >  1:1531181/1‑150 (MQ=255)
                                                                                                                       tGTTATGCAAAATGCTACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcgcgcg                             <  1:834521/150‑1 (MQ=255)
                                                                                                                       tGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCcgcgcg                             >  2:480800/1‑150 (MQ=255)
                                                                                                                         ttATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCa                           >  1:654874/1‑150 (MQ=255)
                                                                                                                          tATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCaa                          >  1:1475385/1‑150 (MQ=255)
                                                                                                                           aTGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAAt                         <  2:48197/150‑1 (MQ=255)
                                                                                                                            tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATc                        >  2:370712/1‑150 (MQ=255)
                                                                                                                            tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGGTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATc                        <  1:488997/150‑1 (MQ=255)
                                                                                                                             gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCa                       >  2:1796607/1‑150 (MQ=255)
                                                                                                                              cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAg                      >  2:678865/1‑150 (MQ=255)
                                                                                                                               aaaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAgg                     >  2:1419696/1‑150 (MQ=255)
                                                                                                                                  aTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAAc                  >  1:1042612/1‑150 (MQ=255)
                                                                                                                                   tGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAAcc                 <  1:1377252/150‑1 (MQ=255)
                                                                                                                                     caacaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCg               >  1:1856889/1‑150 (MQ=255)
                                                                                                                                       acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGcc             <  2:233598/150‑1 (MQ=255)
                                                                                                                                       acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGcc             <  2:226487/150‑1 (MQ=255)
                                                                                                                                         cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCaa           >  1:1892141/3‑150 (MQ=255)
                                                                                                                                           aGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATa         <  1:714427/150‑1 (MQ=255)
                                                                                                                                           aGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTGGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGACCCCGCCAAtc         <  1:889690/150‑2 (MQ=255)
                                                                                                                                             ccAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCTCGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATacc       <  2:1252915/150‑1 (MQ=255)
                                                                                                                                              cAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCAGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATacca      <  2:1048058/150‑1 (MQ=255)
                                                                                                                                               aGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATaccac     >  1:1861247/1‑150 (MQ=255)
                                                                                                                                               aGTGAAATCCCTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATaccac     <  1:172508/150‑1 (MQ=255)
                                                                                                                                                gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATaccacc    >  1:1045972/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTCGCTGTTCTGAGCTTAACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAAACTTACGCGAGTAGCCGCGCGCAATCAGGAACCCGCCAATACCACCGA  >  NC_000913/2707172‑2707467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: