Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,073 A→T 6.7% noncoding (85/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,0730AT 6.7% 361.1 / 12.4 138noncoding (85/1542 nt)rrsG16S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base A (61/66);  new base T (4/5);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

Only 100 of 152 total aligned reads displayed.

GGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGT  >  NC_000913/2730924‑2731221
                                                                                                                                                     |                                                                                                                                                    
ggCACATCCGATGGCAAGAGGCCCGAAGGTCCGCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca                                                                                                                                                      <  2:809879/150‑1 (MQ=255)
ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca                                                                                                                                                      <  1:1910225/150‑1 (MQ=39)
ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca                                                                                                                                                      >  1:1698651/1‑150 (MQ=39)
  cacaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg                                                                                                                                                    <  1:227488/150‑1 (MQ=39)
   acaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGGGACATTATGCGGTATTAGCTACAGTTTCCAGTAGGTTTCCCCCTCCATCCGGCGGCTTCCCAGACATTACTCCCCCGTCAGCCCCTCGTCAGCGAAGCCGCTAgc                                                                                                                                                   >  2:388042/1‑150 (MQ=11)
     aTCCTATGGCAAGAGGCCCGAAGGTCGCCCTGTTTGGTCTTGCGACATTATGCGGTATTAGCGACCGTTTCCAGTAGTTATACCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCTCTCGTCAGCGAAGCAGCAAgctg                                                                                                                                                 <  1:302106/150‑1 (MQ=11)
      accGATGGCAAGAGGCCCGCATGGCCCCCTCTTTGGTCTTGCGACATTAAGCGGTTTTAGCTACCGTTTCCAGTAGTTATACCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctgc                                                                                                                                                <  1:558691/149‑1 (MQ=14)
       ccGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCAACCGTTTCCAGTAGTAATCCCCCTCCATCAGGCAGCTTCACAGACATTACTCACCAGTCCCCCACTCGTCAGCGAAGCAGCAAgctggt                                                                                                                                               >  1:1065229/1‑148 (MQ=11)
          aTGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTcc                                                                                                                                            <  1:1381380/150‑1 (MQ=39)
           tGGCCAGAGGCCCGAAGGTCCCCCTCTTTGGGCTTGCGTCATTATGCGGTTTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACTTTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCt                                                                                                                                           <  2:1006641/150‑1 (MQ=14)
           tGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGGCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                                                                           <  1:1557896/150‑3 (MQ=255)
            gggaaGAGGCCCGAAGGTCGCGCTCTTTGGTCTTGCGACATTATGCTGTTTTTGCTAACGTTTCCAGTAGTTATCCCCCTCCATCCGGCAGCTTCCCAGAAATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                                                                          <  2:1047363/147‑4 (MQ=14)
            ggaaaGAGGCCCGAAGGTCCCCCTCTTTGGTGTTGCGACTTTATGCGGTTTTAGCTTCCGTTTCCAGTAGTTATCCCCCTCCATCATGCAGCTTCCGAGACATTACTCACCCTTCAGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTg                                                                                                                                          <  1:425475/147‑1 (MQ=11)
            ggCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                                                                          <  2:1622073/150‑4 (MQ=255)
            ggCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                                                                          <  2:480263/150‑4 (MQ=255)
              cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGtt                                                                                                                                        >  2:512367/1‑150 (MQ=39)
              cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGtt                                                                                                                                        <  2:950335/150‑1 (MQ=39)
              cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCACTCCATCAGGCAGCTTCCCAGCCATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGATGGTTCCGGtt                                                                                                                                        >  1:447227/1‑150 (MQ=11)
                 gagGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTAcc                                                                                                                                     >  2:769900/1‑150 (MQ=39)
                   ggCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt                                                                                                                                   >  1:97482/1‑150 (MQ=39)
                   ggCACGAAGGTCCACCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCATCTTCCCATTCATTACTCAACCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt                                                                                                                                   <  1:1239717/150‑1 (MQ=14)
                     cccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc                                                                                                                                 <  1:710395/150‑1 (MQ=39)
                     cccGAAGGTCCCCCTCTTTGGTCTTGCGACATTAAGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCACTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCCGCGAAGCAGCAAGCTGCTGCCGGTTAcgggtc                                                                                                                                 >  2:1036585/1‑145 (MQ=14)
                      ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                                                                                >  1:564828/1‑150 (MQ=39)
                      ccGAAGGGCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                                                                                <  2:138764/150‑1 (MQ=255)
                        gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                                                                              >  2:1218963/1‑150 (MQ=39)
                        gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                                                                              >  1:905048/1‑150 (MQ=39)
                           ggTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTg                                                                                                                           <  2:1341362/150‑1 (MQ=39)
                             tCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa                                                                                                                         <  2:567793/150‑1 (MQ=39)
                             tCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa                                                                                                                         <  1:800411/150‑1 (MQ=39)
                              cccccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCTTCAGCGAAGCAGCAAGCTGCTTCCGGTTACCGTTCGACTTGCAt                                                                                                                        >  1:1127539/1‑150 (MQ=255)
                                 ccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCGGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtgtg                                                                                                                     <  1:1880109/150‑1 (MQ=255)
                                 ccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTGACCGTTCGACTTGCAtgtg                                                                                                                     >  1:1363713/1‑150 (MQ=255)
                                  ctctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGGTTCCTGTTACCGTTCGACTTGCATgtgt                                                                                                                    >  1:1967773/1‑150 (MQ=37)
                                   gcattGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                                                                   <  2:162780/147‑1 (MQ=255)
                                    ctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTa                                                                                                                  <  2:1110152/150‑1 (MQ=39)
                                       tGGTCTTGCGACATTATGCGGTTTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                                                                               <  1:1212799/150‑1 (MQ=255)
                                       tGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                                                                               <  2:578635/150‑1 (MQ=39)
                                        ggTCTTGCGACATTATGCGGTATTAGCTTCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGGCAGCGGCTTCCTGTTACCGTTCGACGTGCATGTGTTAGGcc                                                                                                              >  1:174931/1‑150 (MQ=14)
                                              gCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccgc                                                                                                        >  2:1948942/1‑150 (MQ=39)
                                               cGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCGAGCTGCTTACTGTTACCGTTCGACTTGCAGGTGTTAGGCCTgccgcc                                                                                                       >  1:306218/1‑150 (MQ=255)
                                               cGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccgcc                                                                                                       >  1:1204764/1‑150 (MQ=39)
                                                       tGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCCGCGAAGCAGCAAGCTGCTTCCTGTTTCCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                                                                               >  2:499684/1‑150 (MQ=255)
                                                       tGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                                                                               >  1:643567/1‑150 (MQ=37)
                                                        gCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCaa                                                                                              >  2:1343459/1‑150 (MQ=37)
                                                        gCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCaa                                                                                              <  1:1845849/150‑1 (MQ=37)
                                                         cGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAAt                                                                                             <  2:1764974/150‑1 (MQ=37)
                                                          ggTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc                                                                                            <  1:1649689/150‑1 (MQ=37)
                                                             aTTAGCTACCGTTTCCAGTAGTTATCCCCCGCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCAGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCATGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGa                                                                                         >  1:1377/1‑150 (MQ=255)
                                                                 gCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGCGCCa                                                                                     >  2:410833/1‑150 (MQ=37)
                                                                  cTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCCCCCGTCCGCCCCTCGTCAGCGACGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCATTCTGAGCCAt                                                                                    >  2:121355/1‑150 (MQ=255)
                                                                       gTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCACAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTAGCATGACTTAGGCCAGCCGCCAGCGTTCAATCTGAGCCATGATCa                                                                               >  1:940946/1‑150 (MQ=11)
                                                                          tCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGGTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCCATCTGCGCCATGATCAAAc                                                                            >  2:154790/1‑150 (MQ=255)
                                                                           ccAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACACGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGGTAGACGTGCATGTGTTAGGCCGGCCGCCCGCGTTCAATCTGAGCCATGATCAAAca                                                                           >  2:1501054/1‑149 (MQ=11)
                                                                              gtagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACATGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCtt                                                                        <  1:284039/150‑1 (MQ=37)
                                                                                  ttATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCTCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                                                                    >  1:481703/1‑150 (MQ=37)
                                                                                    aTCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAttt                                                                  >  1:737073/1‑150 (MQ=37)
                                                                                      cccccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCCGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaa                                                                >  2:702204/1‑150 (MQ=37)
                                                                                         cctccctcAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg                                                             <  1:1466847/150‑1 (MQ=37)
                                                                                         ccTCCATCAGGCAGCTGCCCAGACATTACTCACCCGTCCGCCACGCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg                                                             <  1:1459914/150‑1 (MQ=255)
                                                                                           tCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGtt                                                           >  2:1484371/1‑150 (MQ=37)
                                                                                            ccATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTAAAAAGttt                                                          <  2:1169142/150‑1 (MQ=37)
                                                                                                cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg                                                      >  2:77171/1‑150 (MQ=37)
                                                                                                    cAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCa                                                  <  2:1134299/150‑1 (MQ=37)
                                                                                                           ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAAtt                                           >  2:1555603/1‑150 (MQ=34)
                                                                                                           ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGATCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAAtt                                           <  1:805416/150‑1 (MQ=21)
                                                                                                             aGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTaa                                         >  2:802522/1‑150 (MQ=34)
                                                                                                               aCATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAAc                                       <  2:1283415/150‑1 (MQ=34)
                                                                                                                cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCCATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGCATTAAAcg                                      >  1:1007121/1‑149 (MQ=21)
                                                                                                                cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGAAAGCTGGTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCAGCCAGCGTTCAATATGAGCCATGATCAAACTCTTGAAATTAAAAGTTTGATGCTCAAAGAATTAAACt                                      >  2:91529/1‑150 (MQ=11)
                                                                                                                     cTCACCCGTCCGCCACTCGTCAGCGAAGCAGAAAGCTGCTTCCTGTTACAGTTCGACTTGCATGTGTTATGACTGCCGCCAGCGTTCAATCTGAGCCATGATGAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTa                                 >  2:147772/1‑150 (MQ=11)
                                                                                                                       cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                               <  1:1325206/150‑1 (MQ=34)
                                                                                                                       cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                               >  1:874347/1‑150 (MQ=34)
                                                                                                                       cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                               <  1:1764289/150‑1 (MQ=34)
                                                                                                                        aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCTGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                              <  1:437983/150‑1 (MQ=21)
                                                                                                                        aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                              <  2:1501379/150‑1 (MQ=34)
                                                                                                                        aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGGTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                              <  2:1214731/150‑1 (MQ=21)
                                                                                                                         cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGa                             >  1:1855352/1‑150 (MQ=34)
                                                                                                                          ccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGaa                            >  2:228641/1‑150 (MQ=34)
                                                                                                                            gTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAAtt                          >  2:1331971/1‑150 (MQ=34)
                                                                                                                            gTCCGCCACTCGTCAGAGAAGCAGCATGCTGCTTCCTGTTACCGTTCGACTTGCATGTGATAGGCCTGCGGCCAGCTTTCAATATGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAAtt                          <  2:119006/150‑1 (MQ=11)
                                                                                                                                   aCTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGtt                   <  2:1377/150‑1 (MQ=34)
                                                                                                                                      cGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCAACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCAc                >  2:841250/1‑150 (MQ=21)
                                                                                                                                        tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCActc              >  2:529963/1‑150 (MQ=34)
                                                                                                                                        tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCActc              >  1:873889/1‑150 (MQ=34)
                                                                                                                                         cAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCActct             >  2:655712/1‑150 (MQ=34)
                                                                                                                                          caggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCtt            >  2:207456/4‑150 (MQ=255)
                                                                                                                                           gCGAATCAGCAAGCTGCTTCCTGTTACCGTTCGACTAGCATGTGTTAGGACTGCCGCCAGCGTTCAATCTGAGCCATGAGCAAACTCTTCAATTTAAAAGTGTGATGCTCAAAGAATTATACTTCGTAATGAATTACGTGTTCACTCTTg           <  2:1241311/150‑1 (MQ=11)
                                                                                                                                           gCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTg           >  1:1716834/1‑150 (MQ=34)
                                                                                                                                             gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTgag         <  2:1452056/150‑1 (MQ=34)
                                                                                                                                             gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTgag         <  1:77171/150‑1 (MQ=34)
                                                                                                                                                gcagcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACt      >  2:254522/1‑150 (MQ=32)
                                                                                                                                                  agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTg    >  2:1432032/1‑150 (MQ=32)
                                                                                                                                                  agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCATCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTg    <  2:253235/150‑1 (MQ=17)
                                                                                                                                                  agcaAGCTGCTTCCTGATACCGTTCGACTAGCATGTGTTAGGCATGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTACCTAAAGAATAACGTGTTCACTCTTGAGACTTg    >  2:1977878/1‑150 (MQ=11)
                                                                                                                                                   gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg   <  2:564828/150‑1 (MQ=32)
                                                                                                                                                   gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg   <  2:481703/150‑1 (MQ=32)
                                                                                                                                                   gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg   <  1:2006942/150‑1 (MQ=32)
                                                                                                                                                   gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg   >  1:1888515/1‑150 (MQ=32)
                                                                                                                                                    caAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGt  <  2:1133918/150‑1 (MQ=32)
                                                                                                                                                     |                                                                                                                                                    
GGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGT  >  NC_000913/2730924‑2731221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: