Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,731,074 A→T 5.5% noncoding (84/1542 nt) rrsG ← 16S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,731,0740AT 5.5% 383.9 / 9.5 149noncoding (84/1542 nt)rrsG16S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base A (68/72);  new base T (3/6);  total (71/78)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

Only 100 of 150 total aligned reads displayed.

CACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTAT  >  NC_000913/2730926‑2731223
                                                                                                                                                    |                                                                                                                                                     
cacaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg                                                                                                                                                      <  1:227488/150‑1 (MQ=39)
 acaTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGGGACATTATGCGGTATTAGCTACAGTTTCCAGTAGGTTTCCCCCTCCATCCGGCGGCTTCCCAGACATTACTCCCCCGTCAGCCCCTCGTCAGCGAAGCCGCTAgc                                                                                                                                                     >  2:388042/1‑150 (MQ=11)
   aTCCTATGGCAAGAGGCCCGAAGGTCGCCCTGTTTGGTCTTGCGACATTATGCGGTATTAGCGACCGTTTCCAGTAGTTATACCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCTCTCGTCAGCGAAGCAGCAAgctg                                                                                                                                                   <  1:302106/150‑1 (MQ=11)
    accGATGGCAAGAGGCCCGCATGGCCCCCTCTTTGGTCTTGCGACATTAAGCGGTTTTAGCTACCGTTTCCAGTAGTTATACCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctgc                                                                                                                                                  <  1:558691/149‑1 (MQ=14)
     ccGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCAACCGTTTCCAGTAGTAATCCCCCTCCATCAGGCAGCTTCACAGACATTACTCACCAGTCCCCCACTCGTCAGCGAAGCAGCAAgctggt                                                                                                                                                 >  1:1065229/1‑148 (MQ=11)
        aTGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTcc                                                                                                                                              <  1:1381380/150‑1 (MQ=39)
         tGGCCAGAGGCCCGAAGGTCCCCCTCTTTGGGCTTGCGTCATTATGCGGTTTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACTTTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCt                                                                                                                                             <  2:1006641/150‑1 (MQ=14)
         tGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGGCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgc                                                                                                                                             <  1:1557896/150‑3 (MQ=255)
          gggaaGAGGCCCGAAGGTCGCGCTCTTTGGTCTTGCGACATTATGCTGTTTTTGCTAACGTTTCCAGTAGTTATCCCCCTCCATCCGGCAGCTTCCCAGAAATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                                                                            <  2:1047363/147‑4 (MQ=14)
          ggaaaGAGGCCCGAAGGTCCCCCTCTTTGGTGTTGCGACTTTATGCGGTTTTAGCTTCCGTTTCCAGTAGTTATCCCCCTCCATCATGCAGCTTCCGAGACATTACTCACCCTTCAGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTg                                                                                                                                            <  1:425475/147‑1 (MQ=11)
          ggCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCTTGCTGCTTcgct                                                                                                                                            <  2:1622073/150‑4 (MQ=255)
            cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGtt                                                                                                                                          <  2:950335/150‑1 (MQ=39)
            cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGtt                                                                                                                                          >  2:512367/1‑150 (MQ=39)
            cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCACTCCATCAGGCAGCTTCCCAGCCATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGATGGTTCCGGtt                                                                                                                                          >  1:447227/1‑150 (MQ=11)
             aaGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTa                                                                                                                                         <  1:1907221/150‑1 (MQ=39)
                 ggCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt                                                                                                                                     >  1:97482/1‑150 (MQ=39)
                 ggCACGAAGGTCCACCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCATCTTCCCATTCATTACTCAACCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGt                                                                                                                                     <  1:1239717/150‑1 (MQ=14)
                   cccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc                                                                                                                                   <  1:710395/150‑1 (MQ=39)
                   cccGAAGGTCCCCCTCTTTGGTCTTGCGACATTAAGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCACTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCCGCGAAGCAGCAAGCTGCTGCCGGTTAcgggtc                                                                                                                                   >  2:1036585/1‑145 (MQ=14)
                    ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                                                                                  >  1:564828/1‑150 (MQ=39)
                    ccGAAGGGCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg                                                                                                                                  <  2:138764/150‑1 (MQ=255)
                      gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                                                                                <  1:1858301/150‑1 (MQ=39)
                      gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc                                                                                                                                >  1:905048/1‑150 (MQ=39)
                         ggTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCTCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTg                                                                                                                             >  2:1114331/1‑150 (MQ=255)
                         ggTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTg                                                                                                                             <  2:1341362/150‑1 (MQ=39)
                           tCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACTCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa                                                                                                                           >  2:791583/1‑150 (MQ=255)
                           tCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCa                                                                                                                           <  1:800411/150‑1 (MQ=39)
                            cccccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCTTCAGCGAAGCAGCAAGCTGCTTCCGGTTACCGTTCGACTTGCAt                                                                                                                          >  1:1127539/1‑150 (MQ=255)
                            cccccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAt                                                                                                                          <  2:240999/150‑1 (MQ=39)
                             ccccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                                                                         >  1:1984231/1‑150 (MQ=39)
                             ccccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCAtg                                                                                                                         <  2:522625/150‑1 (MQ=39)
                               ccTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTGACCGTTCGACTTGCAtgtg                                                                                                                       >  1:1363713/1‑150 (MQ=255)
                                 gcattGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGtt                                                                                                                     <  2:162780/147‑1 (MQ=255)
                                  ctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTa                                                                                                                    <  2:1110152/150‑1 (MQ=39)
                                    ttGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAgg                                                                                                                  <  1:1767170/150‑1 (MQ=39)
                                     tGGTCTTGCGACATTATGCGGTTTTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                                                                                 <  1:1212799/150‑1 (MQ=255)
                                     tGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGc                                                                                                                 <  2:578635/150‑1 (MQ=39)
                                             cGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCGAGCTGCTTACTGTTACCGTTCGACTTGCAGGTGTTAGGCCTgccgcc                                                                                                         >  1:306218/1‑150 (MQ=255)
                                             cGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccgcc                                                                                                         >  1:1204764/1‑150 (MQ=39)
                                             cGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTgccgcc                                                                                                         <  2:56736/150‑1 (MQ=39)
                                                 aTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCg                                                                                                     <  2:1155394/150‑1 (MQ=38)
                                                     tGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCa                                                                                                 >  1:643567/1‑150 (MQ=37)
                                                      gCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCaa                                                                                                >  2:1343459/1‑150 (MQ=37)
                                                       cGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAAt                                                                                               <  2:1764974/150‑1 (MQ=37)
                                                        ggTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATc                                                                                              <  1:1649689/150‑1 (MQ=37)
                                                           aTTAGCTACCGTTTCCAGTAGTTATCCCCCGCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCAGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCATGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGa                                                                                           >  1:1377/1‑150 (MQ=255)
                                                             tAGCTACCGTTTCCAGTAGTTATCCCCTTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGc                                                                                         <  2:484672/150‑1 (MQ=37)
                                                                  aCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGa                                                                                    >  2:839569/1‑150 (MQ=37)
                                                                    cGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATc                                                                                  <  2:58859/150‑1 (MQ=37)
                                                                     gTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCACAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTAGCATGACTTAGGCCAGCCGCCAGCGTTCAATCTGAGCCATGATCa                                                                                 >  1:940946/1‑150 (MQ=11)
                                                                        tCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGGTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCCATCTGCGCCATGATCAAAc                                                                              >  2:154790/1‑150 (MQ=255)
                                                                         ccAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACACGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGGTAGACGTGCATGTGTTAGGCCGGCCGCCCGCGTTCAATCTGAGCCATGATCAAAca                                                                             >  2:1501054/1‑149 (MQ=11)
                                                                            gtagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACATGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCtt                                                                          <  1:284039/150‑1 (MQ=37)
                                                                              agtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCa                                                                        >  2:707812/1‑150 (MQ=37)
                                                                                ttATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCTCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAt                                                                      >  1:481703/1‑150 (MQ=37)
                                                                                 tATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATTATCAAACTCTTCAAtt                                                                     <  2:121501/150‑1 (MQ=37)
                                                                                  aTCCCCCTCCATCCCGCAGCTTCCCACACAATACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAttt                                                                    <  2:411901/150‑1 (MQ=255)
                                                                                  aTCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAAttt                                                                    >  1:737073/1‑150 (MQ=37)
                                                                                    cccccTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCCGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTaa                                                                  >  2:702204/1‑150 (MQ=37)
                                                                                       cctccctcAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg                                                               <  1:1466847/150‑1 (MQ=37)
                                                                                       ccTCCATCAGGCAGCTGCCCAGACATTACTCACCCGTCCGCCACGCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAg                                                               <  1:1459914/150‑1 (MQ=255)
                                                                                         tCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGtt                                                             >  2:1484371/1‑150 (MQ=37)
                                                                                              cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg                                                        <  2:1204763/150‑1 (MQ=37)
                                                                                              cAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATg                                                        >  2:77171/1‑150 (MQ=37)
                                                                                                  cAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCa                                                    <  2:1134299/150‑1 (MQ=37)
                                                                                                         ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAAtt                                             >  2:1555603/1‑150 (MQ=34)
                                                                                                         ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGATCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAAtt                                             <  1:805416/150‑1 (MQ=21)
                                                                                                             aCATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAAc                                         <  2:1283415/150‑1 (MQ=34)
                                                                                                              cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCCATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGCATTAAAcg                                        >  1:1007121/1‑149 (MQ=21)
                                                                                                                   cTCACCCGTCCGCCACTCGTCAGCGAAGCAGAAAGCTGCTTCCTGTTACAGTTCGACTTGCATGTGTTATGACTGCCGCCAGCGTTCAATCTGAGCCATGATGAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTa                                   >  2:147772/1‑150 (MQ=11)
                                                                                                                     cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                                 <  1:1325206/150‑1 (MQ=34)
                                                                                                                     cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                                 <  1:1764289/150‑1 (MQ=34)
                                                                                                                     cACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAAt                                 >  1:874347/1‑150 (MQ=34)
                                                                                                                      aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCTGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                                <  1:437983/150‑1 (MQ=21)
                                                                                                                      aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                                <  2:1501379/150‑1 (MQ=34)
                                                                                                                      aCCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGGTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATg                                <  2:1214731/150‑1 (MQ=21)
                                                                                                                       cccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGa                               >  1:1855352/1‑150 (MQ=34)
                                                                                                                        ccGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGaa                              >  2:228641/1‑150 (MQ=34)
                                                                                                                          gTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAAtt                            >  2:1331971/1‑150 (MQ=34)
                                                                                                                          gTCCGCCACTCGTCAGAGAAGCAGCATGCTGCTTCCTGTTACCGTTCGACTTGCATGTGATAGGCCTGCGGCCAGCTTTCAATATGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAAtt                            <  2:119006/150‑1 (MQ=11)
                                                                                                                                 aCTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGtt                     <  2:1377/150‑1 (MQ=34)
                                                                                                                                 aCTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGtt                     <  2:1984230/150‑1 (MQ=34)
                                                                                                                                    cGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCAACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCAc                  >  2:841250/1‑150 (MQ=21)
                                                                                                                                      tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCActc                >  1:873889/1‑150 (MQ=34)
                                                                                                                                       cAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCActct               <  2:306218/150‑1 (MQ=34)
                                                                                                                                         gCGAATCAGCAAGCTGCTTCCTGTTACCGTTCGACTAGCATGTGTTAGGACTGCCGCCAGCGTTCAATCTGAGCCATGAGCAAACTCTTCAATTTAAAAGTGTGATGCTCAAAGAATTATACTTCGTAATGAATTACGTGTTCACTCTTg             <  2:1241311/150‑1 (MQ=11)
                                                                                                                                         gCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTg             >  1:1716834/1‑150 (MQ=34)
                                                                                                                                          ggAAGCAGCTTGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTga            >  1:1727494/2‑150 (MQ=21)
                                                                                                                                           gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTgag           <  2:1452056/150‑1 (MQ=34)
                                                                                                                                           gAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTgag           <  1:77171/150‑1 (MQ=34)
                                                                                                                                            aaGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTgaga          >  2:1876226/1‑150 (MQ=34)
                                                                                                                                              gcagcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACt        >  2:254522/1‑150 (MQ=32)
                                                                                                                                                agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTg      >  2:1432032/1‑150 (MQ=32)
                                                                                                                                                agcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTg      <  2:922461/150‑1 (MQ=32)
                                                                                                                                                agcaAGCTGCTTCCTGATACCGTTCGACTAGCATGTGTTAGGCATGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTACCTAAAGAATAACGTGTTCACTCTTGAGACTTg      >  2:1977878/1‑150 (MQ=11)
                                                                                                                                                 gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg     <  2:481703/150‑1 (MQ=32)
                                                                                                                                                 gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg     <  2:564828/150‑1 (MQ=32)
                                                                                                                                                 gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg     <  1:2006942/150‑1 (MQ=32)
                                                                                                                                                 gcaAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTgg     >  1:1888515/1‑150 (MQ=32)
                                                                                                                                                    aGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTAt  >  2:1261960/1‑150 (MQ=32)
                                                                                                                                                    |                                                                                                                                                     
CACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCAATTTAAAAGTTTGATGCTCAAAGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTAT  >  NC_000913/2730926‑2731223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: